High-pressure(HP)or ultrahigh-pressure(UHP)rutile-quartz veins that form at mantle depths due to fluid-rock interaction can be used to trace the properties and behavior of natural fluids in subduction zones.To explore...High-pressure(HP)or ultrahigh-pressure(UHP)rutile-quartz veins that form at mantle depths due to fluid-rock interaction can be used to trace the properties and behavior of natural fluids in subduction zones.To explore the fluid flow and the associated element mobility during deep subduction and exhumation of the continental crust,we investigated the major and trace elements of Ti-rich minerals.Additionally,U–Pb dating,trace element contents,and Lu–Hf isotopic composition of zircon grains in the UHP eclogite and associated rutile-quartz veins were examined in the North Qaidam UHP metamorphic belt,Yuka terrane.The zircon grains in the rutile-quartz veins have unzoned or weak oscillatory zonings,and show low Th/U ratios,steep chondrite-normalized patterns of heavy rare earth elements(HREEs),and insignificant negative Eu anomalies,indicating their growth in metamorphic fluids.These zircon grains formed in 4313 Ma,which is consistent with the 4322 Ma age of the host eclogite.As for the zircons in the rutile-quartz veins,they showed steep HREE patterns on one hand,and were different from the zircons present in the host eclogite on the other.This demonstrates that their formation might have been related to the breakdown of the early stage of garnet,which corresponds to the abundance of fluids during the early exhumation stage.The core-rim profile analyses of rutile recorded a two-stage rutile growth across a large rutile grain;the rutile core has higher Nb,Ta,W,and Zr contents and lower Nb/Ta ratios than the rim,indicating that the rutile domains grew in different metamorphic fluids from the core towards the rim.The significant enrichment of high field strength elements(HFSEs)in the rutile core suggests that the peak fluids have high solubility and transportation capacity of these HFSEs.Furthermore,variations in the Nb vs.Cr trends in rutile indicate a connection of rutile to mafic protolith.The zircon grains from both the rutile-quartz veins and the host eclogite have similar Hf isotopic compositions,indicating that the vein-forming fluids are internally derived from the host eclogite.These fluids accumulated in the subduction channel and were triggered by local dehydration of the deeply subducted eclogite during the early exhumation conditions.展开更多
High-silica(SiO_(2)>70 wt.%)granites(HSGs)are the main source of W,Sn,and rare metals.However,abundant HSGs,temporally,spatially,and genetically associated with Pb-Zn mineralization,in the Lhasa terrane(LT),provide...High-silica(SiO_(2)>70 wt.%)granites(HSGs)are the main source of W,Sn,and rare metals.However,abundant HSGs,temporally,spatially,and genetically associated with Pb-Zn mineralization,in the Lhasa terrane(LT),provided an ideal opportunity to study the key factors responsible for Pb-Zn enrichment,instead of W-Sn enrichment.Here we contribute to this topic through U-Pb dating of zircon and garnet,and whole-rock and Sr-Nd-Hf isotopic geochemistry of ore-related quartz porphyries in the Bangbule deposit and compared these results with published data from large and giant Pb-Zn and W deposits in the LT.The magmatism-alteration-mineralization event in the Bangbule deposit was recorded by robust zircon U-Pb ages of 77.3±0.9 Ma and hydrothermal garnet U-Pb ages of 75.7±4.8 Ma,which is 10-15 Ma earlier than the main Paleocene metallogenic event and the first record of late Cretaceous Pb-Zn polymetallic mineralization in the LT.The late Cretaceous-Paleocene magmatism and mineralization events are a response to the subduction of Neotethyan oceanic lithosphere,which occurred as a result of the collision of the Indian and Asian plates.These HSGs related to Pb-Zn mineralization,with high totalalkalis and low magnesian contents,are enriched in Ba,Th,and Rb,but depleted in Ti,Eu,Sr,and P.They belong to either the S-type,or I-type granites.The Sr-Nd-Hf isotopic compositions of the Pb-Zn mineralized granites demonstrate that they were generated by the partial melting of Proterozoic basement with or without mantle-derived melt input.This was consistent with the postulated source of W enrichment in the LT.The Pb-Zn and W related granites have similar zircon-Ti-saturation temperatures,comparable low whole-rock Fe_(2)O_(3)/FeO ratios,and zircon oxygen fugacity.This indicated that the Pb-Zn-W enrichment in the high-silica magma system could be attributed to a relatively reduced magma.The Pb-Zn related HSGs,abundant quartz and feldspar phenocrysts,and weak fractionation of twin-elements in wholerock analysis,can be used to reconstruct a model of the magma reservoir.We postulate that these features could be reproduced by silica-rich crystal accumulation in a magma reservoir,with a loss of magmatic fluids.The magma associated with W mineralization exhibited a higher level of differentiation compared to the Pb-Zn related magma;however,different groups of zircon texture with varying rare earth elements and concomitance of rare earth elements tetrad effect and high fractionation of twin-elements in wholerock are formed by a magmatic-hydrothermal transition in highly evolved system.As the source and oxygen fugacities of the Pb-Zn and W related magmas are similar,the absence of a giant W-Sn deposit in the LT may indicate that parent magmas with a low degree of evolution and magmatic-hydrothermal transition are not conducive to their formation.This implies that the rocks that originated as highly evolved silicaterich parent magmas,with a high degree of magmatic-hydrothermal alteration,would need to be targeted for W-Sn mineral exploration in the LT.In summary,our results emphasize that variations in chemical differentiation and the evolution of high-silica magmatic-hydrothermal systems can lead to differences in Pb-Zn and W enrichment.This has implications for the evaluation of the mineral potential of high-silica granites and hence their attractiveness as targets for mineral exploration.展开更多
Objective:To explore the inhibitory effect of curcumin on colon cancer in vitro.Methods:Human colon cancer cell lines HT29 and SW620 were cultured in vitro,which were divided into test group and control group.Cells in...Objective:To explore the inhibitory effect of curcumin on colon cancer in vitro.Methods:Human colon cancer cell lines HT29 and SW620 were cultured in vitro,which were divided into test group and control group.Cells in the test groups were treated with curcumin at different concentrations,while the control groups were treated with dimethylsulfoxide(DMSO).Subsequently,methyl thiazolyl tetrazolium(MTT)assay,were carried out to estimate cellular proliferative activity.Flow cytometry using annexin-V/propidium iodide(PI)staining was performed to detect cell apoptosis.Formation of autophagosomes in the cytoplasm in colon cancer cells were detected by TEM(transmission electron microscopy analysis),and Western blot to measure the expressions of proteins related to apoptosis and autophagy.Results:MTT assay showed that curcumin inhibited the proliferative activity of both HT29 and SW620 cells,significantly different from the control group(P<0.001).The halfmaximal inhibitory concentration(IC50)of curcumin at 24 hours was 20μM for both HT29 and SW620 cells.Annexin-V FITC/PI double labeling showed that,After treatment with curcumin of 20μM for 24 hours,a significant increase was observed in apoptosis rate in both HT219 cells((10.00±0.60)%vs.(4.00±0.70)%,P<0.01;(10.50±0.40)%vs.(4.30±0.26)%,P<0.01)compared with the control cells.TEM analysis showed that curcumin increased the accumulation of autophagosomes in the cytoplasm in HT29 and SW620 cells.Western blot showed an increase in the expressions of apoptosis-related proteins including cleaved caspase-3 and cleaved poly ADP-ribose polymerase(PARP)but decrease of P62 protein in both HT29 and SW620 cells after 24-hour treatment with 20μM curcumin for 24 hours compared with the control groups.Conclusion:Curcumin can inhibit the proliferation,induce apoptosis and autophagy in human colon cancer cells.展开更多
Tibet, which is rich in mineral resources, is a treasure trove for geological explorers. However, prospecting work has been slow, especially in the western part, due to the precipitous terrain, changeable climate and ...Tibet, which is rich in mineral resources, is a treasure trove for geological explorers. However, prospecting work has been slow, especially in the western part, due to the precipitous terrain, changeable climate and low access. Hence, modern advanced field analytical technology and effective data processing methods play significant roles in rapid and efficient exploration in Tibet. In this paper, spectrum-area fractal modeling and portable X-ray fluorescence analysis(pXRFA) were used to identify and verify geochemical anomalies associated with Ag-Pb-Zn mineralization based on a stream-sediment dataset of 39 elements in the Dajiacuo-Xurucuo region of western Tibet. First, staged factor analysis(SFA) was used to obtain the Ag-Pb-Zn-Cd geochemical assemblage. Second, the first-factor pattern obtained using SFA was dissociated by a spectrum-area(S-A) fractal model and a digital elevation model(DEM)-based geochemical model(DGM) was constructed. Finally, the sections of Ag, Cd, Pb, and Zn were obtained using pXRFA. The results show that Ag-Pb-Zn-Cd enrichment zones were mostly located around the contact belt of volcanic rocks and intrusions, or along SE-NW trending faults. Considering the variable terrain and catchment basin, the extension of long axes of Ag-Pb-Zn-Cd anomalies into higher elevation areas that are favorable for Ag-Pb-Zn mineralization should be investigated. Anomaly maps created with the aid of a DGM show promising potential for mineralization in the Dajiacuo-Xurucuo region, and abundant Ag-Pb-Zn mineralization was identified with the assistance of pXRFA in the source areas for the geochemical anomalies in the Dajiacuo. We conclude that SFA and the S-A fractal model constitute a valid tool to identify or verify geochemical anomalies in areas of low-density stream-sediment sampling. The pXRFA can accurately determine the source of geochemical anomalies and improve anomaly verification efficiency.展开更多
基金funded by the Fundamental Research Funds for National Universities, China University of Geosciences (Wuhan) and China Geological Survey (Grant Nos. 12120113032800, 21201011000150004, DD20190069)
文摘High-pressure(HP)or ultrahigh-pressure(UHP)rutile-quartz veins that form at mantle depths due to fluid-rock interaction can be used to trace the properties and behavior of natural fluids in subduction zones.To explore the fluid flow and the associated element mobility during deep subduction and exhumation of the continental crust,we investigated the major and trace elements of Ti-rich minerals.Additionally,U–Pb dating,trace element contents,and Lu–Hf isotopic composition of zircon grains in the UHP eclogite and associated rutile-quartz veins were examined in the North Qaidam UHP metamorphic belt,Yuka terrane.The zircon grains in the rutile-quartz veins have unzoned or weak oscillatory zonings,and show low Th/U ratios,steep chondrite-normalized patterns of heavy rare earth elements(HREEs),and insignificant negative Eu anomalies,indicating their growth in metamorphic fluids.These zircon grains formed in 4313 Ma,which is consistent with the 4322 Ma age of the host eclogite.As for the zircons in the rutile-quartz veins,they showed steep HREE patterns on one hand,and were different from the zircons present in the host eclogite on the other.This demonstrates that their formation might have been related to the breakdown of the early stage of garnet,which corresponds to the abundance of fluids during the early exhumation stage.The core-rim profile analyses of rutile recorded a two-stage rutile growth across a large rutile grain;the rutile core has higher Nb,Ta,W,and Zr contents and lower Nb/Ta ratios than the rim,indicating that the rutile domains grew in different metamorphic fluids from the core towards the rim.The significant enrichment of high field strength elements(HFSEs)in the rutile core suggests that the peak fluids have high solubility and transportation capacity of these HFSEs.Furthermore,variations in the Nb vs.Cr trends in rutile indicate a connection of rutile to mafic protolith.The zircon grains from both the rutile-quartz veins and the host eclogite have similar Hf isotopic compositions,indicating that the vein-forming fluids are internally derived from the host eclogite.These fluids accumulated in the subduction channel and were triggered by local dehydration of the deeply subducted eclogite during the early exhumation conditions.
基金Fundamental Research Funds for the National Foundation of China(42102058)open fund from the Key Laboratory of Deep-Earth Dynamics of Ministry of Natural Resource,Institute of Geology,Chinese Academy of Geological Sciences (No. J1901-16)Central Universities,and China University of Geosciences (Wuhan)(No.2019132)。
文摘High-silica(SiO_(2)>70 wt.%)granites(HSGs)are the main source of W,Sn,and rare metals.However,abundant HSGs,temporally,spatially,and genetically associated with Pb-Zn mineralization,in the Lhasa terrane(LT),provided an ideal opportunity to study the key factors responsible for Pb-Zn enrichment,instead of W-Sn enrichment.Here we contribute to this topic through U-Pb dating of zircon and garnet,and whole-rock and Sr-Nd-Hf isotopic geochemistry of ore-related quartz porphyries in the Bangbule deposit and compared these results with published data from large and giant Pb-Zn and W deposits in the LT.The magmatism-alteration-mineralization event in the Bangbule deposit was recorded by robust zircon U-Pb ages of 77.3±0.9 Ma and hydrothermal garnet U-Pb ages of 75.7±4.8 Ma,which is 10-15 Ma earlier than the main Paleocene metallogenic event and the first record of late Cretaceous Pb-Zn polymetallic mineralization in the LT.The late Cretaceous-Paleocene magmatism and mineralization events are a response to the subduction of Neotethyan oceanic lithosphere,which occurred as a result of the collision of the Indian and Asian plates.These HSGs related to Pb-Zn mineralization,with high totalalkalis and low magnesian contents,are enriched in Ba,Th,and Rb,but depleted in Ti,Eu,Sr,and P.They belong to either the S-type,or I-type granites.The Sr-Nd-Hf isotopic compositions of the Pb-Zn mineralized granites demonstrate that they were generated by the partial melting of Proterozoic basement with or without mantle-derived melt input.This was consistent with the postulated source of W enrichment in the LT.The Pb-Zn and W related granites have similar zircon-Ti-saturation temperatures,comparable low whole-rock Fe_(2)O_(3)/FeO ratios,and zircon oxygen fugacity.This indicated that the Pb-Zn-W enrichment in the high-silica magma system could be attributed to a relatively reduced magma.The Pb-Zn related HSGs,abundant quartz and feldspar phenocrysts,and weak fractionation of twin-elements in wholerock analysis,can be used to reconstruct a model of the magma reservoir.We postulate that these features could be reproduced by silica-rich crystal accumulation in a magma reservoir,with a loss of magmatic fluids.The magma associated with W mineralization exhibited a higher level of differentiation compared to the Pb-Zn related magma;however,different groups of zircon texture with varying rare earth elements and concomitance of rare earth elements tetrad effect and high fractionation of twin-elements in wholerock are formed by a magmatic-hydrothermal transition in highly evolved system.As the source and oxygen fugacities of the Pb-Zn and W related magmas are similar,the absence of a giant W-Sn deposit in the LT may indicate that parent magmas with a low degree of evolution and magmatic-hydrothermal transition are not conducive to their formation.This implies that the rocks that originated as highly evolved silicaterich parent magmas,with a high degree of magmatic-hydrothermal alteration,would need to be targeted for W-Sn mineral exploration in the LT.In summary,our results emphasize that variations in chemical differentiation and the evolution of high-silica magmatic-hydrothermal systems can lead to differences in Pb-Zn and W enrichment.This has implications for the evaluation of the mineral potential of high-silica granites and hence their attractiveness as targets for mineral exploration.
基金Youth Science and Technology Project of Hebei Provincial Health Department,Approval number:20181535.
文摘Objective:To explore the inhibitory effect of curcumin on colon cancer in vitro.Methods:Human colon cancer cell lines HT29 and SW620 were cultured in vitro,which were divided into test group and control group.Cells in the test groups were treated with curcumin at different concentrations,while the control groups were treated with dimethylsulfoxide(DMSO).Subsequently,methyl thiazolyl tetrazolium(MTT)assay,were carried out to estimate cellular proliferative activity.Flow cytometry using annexin-V/propidium iodide(PI)staining was performed to detect cell apoptosis.Formation of autophagosomes in the cytoplasm in colon cancer cells were detected by TEM(transmission electron microscopy analysis),and Western blot to measure the expressions of proteins related to apoptosis and autophagy.Results:MTT assay showed that curcumin inhibited the proliferative activity of both HT29 and SW620 cells,significantly different from the control group(P<0.001).The halfmaximal inhibitory concentration(IC50)of curcumin at 24 hours was 20μM for both HT29 and SW620 cells.Annexin-V FITC/PI double labeling showed that,After treatment with curcumin of 20μM for 24 hours,a significant increase was observed in apoptosis rate in both HT219 cells((10.00±0.60)%vs.(4.00±0.70)%,P<0.01;(10.50±0.40)%vs.(4.30±0.26)%,P<0.01)compared with the control cells.TEM analysis showed that curcumin increased the accumulation of autophagosomes in the cytoplasm in HT29 and SW620 cells.Western blot showed an increase in the expressions of apoptosis-related proteins including cleaved caspase-3 and cleaved poly ADP-ribose polymerase(PARP)but decrease of P62 protein in both HT29 and SW620 cells after 24-hour treatment with 20μM curcumin for 24 hours compared with the control groups.Conclusion:Curcumin can inhibit the proliferation,induce apoptosis and autophagy in human colon cancer cells.
基金funded by the Fundamental Research Funds for the Central UniversitiesChina University of Geosciences (Wuhan)(No. 2019132)China Geological Survey (No. DD20190159-33)。
文摘Tibet, which is rich in mineral resources, is a treasure trove for geological explorers. However, prospecting work has been slow, especially in the western part, due to the precipitous terrain, changeable climate and low access. Hence, modern advanced field analytical technology and effective data processing methods play significant roles in rapid and efficient exploration in Tibet. In this paper, spectrum-area fractal modeling and portable X-ray fluorescence analysis(pXRFA) were used to identify and verify geochemical anomalies associated with Ag-Pb-Zn mineralization based on a stream-sediment dataset of 39 elements in the Dajiacuo-Xurucuo region of western Tibet. First, staged factor analysis(SFA) was used to obtain the Ag-Pb-Zn-Cd geochemical assemblage. Second, the first-factor pattern obtained using SFA was dissociated by a spectrum-area(S-A) fractal model and a digital elevation model(DEM)-based geochemical model(DGM) was constructed. Finally, the sections of Ag, Cd, Pb, and Zn were obtained using pXRFA. The results show that Ag-Pb-Zn-Cd enrichment zones were mostly located around the contact belt of volcanic rocks and intrusions, or along SE-NW trending faults. Considering the variable terrain and catchment basin, the extension of long axes of Ag-Pb-Zn-Cd anomalies into higher elevation areas that are favorable for Ag-Pb-Zn mineralization should be investigated. Anomaly maps created with the aid of a DGM show promising potential for mineralization in the Dajiacuo-Xurucuo region, and abundant Ag-Pb-Zn mineralization was identified with the assistance of pXRFA in the source areas for the geochemical anomalies in the Dajiacuo. We conclude that SFA and the S-A fractal model constitute a valid tool to identify or verify geochemical anomalies in areas of low-density stream-sediment sampling. The pXRFA can accurately determine the source of geochemical anomalies and improve anomaly verification efficiency.