Simulation and Experimental Study on Load-bearing Deformation Characteristics of 11R22.5 Vehicle Retreaded Tire

The finite element bearing deformation simulation was implemented on 11.00R22.5 retreaded tires by ANSYS software in the paper in order to further clarify the bearing deformation characteristics of retreaded tires and improve the performance of retreaded tires effectively.The characteristic laws of bearing radial deformation and bearing lateral deformation of retreaded tire and new tires of the same model under different working conditions were obtained through load deformation tests.The radial deformation calculation results,simulation results and measured results of retreaded tires were comparatively analyzed.The calculation formula of bearing radial deformation of retreaded tires was proposed based on the linear regression principle.The difference of bearing deformation characteristics and ground area characteristics of retreaded tires and new tires were comparatively analyzed.The results showed that the radial and lateral deformation of retreaded tires and new tires is increased with the increase of radial load when the tire pressure was constant,and the increase trend is approximately linear.The radial stiffness of retreaded tires is similar to that of new tires under certain tire pressure and low load.The radial stiffness of retreaded tires is larger than that of new tires,and the stiffness difference is increased with the increasing of load under constant tire pressure and high load.Rubber aging phenomenon in retreaded tire carcass have an impact on the bearing deformation characteristics of retreaded tires,thereby producing great impact on the remaining service life of retreaded tires.

Single-cell RNA-seq and chromatin accessibility profiling decipher the heterogeneity of mouseγδT cells

The distinct characteristics ofγδT cells determine their vital roles in the formation of local immune responses and contribute to tissue homeostasis.However,the heterogeneity ofγδT cells across tissues remains unclear.By combining transcriptional and chromatin analyses with a truly unbiased fashion,we constructed a single-cell transcriptome and chromatin accessibility landscape of mouseγδT cells in the lymph,spleen,and thymus.We also revealed the heterogeneity ofγδT1 andγδT17 cells across these tissues and inferred their potential regulatory mechanisms.In the thymus,we reconstructed the developmental trajectory and gained further insights into the signature genes from the mature stage,intermediate stage,and immature stage ofγδT cells on the basis of single-cell RNA sequencing and single-cell assay for transposase-accessible chromatin sequencing data.Notably,a novel Gzma^(+)γδT cell subset was identified with immature properties and only localized to the thymus.Finally,NR1 D1,a circadian transcription factor(TF),was validated as a key and negative regulator ofγδT17 cell differentiation by performing a combined analysis of TF motif enrichment,regulon enrichment,and Nr1 d1 knockout mice.In summary,our data represent a comprehensive mapping on the transcriptome and chromatin accessibility dynamics of mouseγδT cells,providing a valuable resource and reference for future studies onγδT cells.