Larimichthys crocea is a marine fish species cultured in China.Short-term starvation is often applied to improve the quality of cultured L.crocea,and the expression of ghrelin in tissues of stomach,muscle,brain,intest...Larimichthys crocea is a marine fish species cultured in China.Short-term starvation is often applied to improve the quality of cultured L.crocea,and the expression of ghrelin in tissues of stomach,muscle,brain,intestines,liver,and kidney,involved in starvation response,under starvation conditions were studied to understand the effect of starvation on the expression of ghrelin in L.crocea juveniles.The ghrelin expression was tissue-specific,and expression was significantly higher in the stomach compared to other tissues(P<0.01).Additionally,ghrelin expression in different tissues changed along with prolongation of fasting.In the stomach,ghrelin expression levels increased gradually at the beginning of the fast,and then declined after eight days of fasting.Gene expression in the brain and intestines increased at the beginning of the fast,and then decreased with longer fasting time.Interestingly,ghrelin expression declined at the beginning of the fast,then increased with longer fasting in the kidneys and muscles.These results suggest that ghrelin is involved in starvation response in L.crocea juveniles.This study provids insights into ghrelin function and an important reference for the development of reasonable feeding strategies for L.crocea juveniles.展开更多
Multichromophoric boron dipyrromethene （BODIPY） dyes have been efficiently synthesized from a one-pot condensation of acyl chloride with 2,4-dimethylpyrrole and have been converted to energy transfer cassettes throu...Multichromophoric boron dipyrromethene （BODIPY） dyes have been efficiently synthesized from a one-pot condensation of acyl chloride with 2,4-dimethylpyrrole and have been converted to energy transfer cassettes through a one-pot Knoevenagel condensation. Various BODIPYs containing 2-ketopyrrole unit were also isolated as the side products and one of their BF2 complex was also isolated from these condensation reactions. The fluores- cence quantum yields of these phenylene bridged dimers and trimer are much lower than those of their correspond- ing monomers. A significant decrease of the fluorescence quantum yield was observed in most polar solvents for these dimers and trimer, which may be due to a possible symmetry breaking resulted intramolecular charge transfer （ICT） in the excited state. Efficient energy transfer between the donor and acceptor was observed in these energy transfer cassettes, which could be useful as large pseudo-Stokes shift fluorescent dyes with potential applications in diverse fields.展开更多
基金The Key Research and Development Project of Zhejiang Province under contract Nos 2016C02055-7,2017C02013。
文摘Larimichthys crocea is a marine fish species cultured in China.Short-term starvation is often applied to improve the quality of cultured L.crocea,and the expression of ghrelin in tissues of stomach,muscle,brain,intestines,liver,and kidney,involved in starvation response,under starvation conditions were studied to understand the effect of starvation on the expression of ghrelin in L.crocea juveniles.The ghrelin expression was tissue-specific,and expression was significantly higher in the stomach compared to other tissues(P<0.01).Additionally,ghrelin expression in different tissues changed along with prolongation of fasting.In the stomach,ghrelin expression levels increased gradually at the beginning of the fast,and then declined after eight days of fasting.Gene expression in the brain and intestines increased at the beginning of the fast,and then decreased with longer fasting time.Interestingly,ghrelin expression declined at the beginning of the fast,then increased with longer fasting in the kidneys and muscles.These results suggest that ghrelin is involved in starvation response in L.crocea juveniles.This study provids insights into ghrelin function and an important reference for the development of reasonable feeding strategies for L.crocea juveniles.
基金This work is supported by the National Natural Science Foundation of China （Grant Nos. 21372011, 21402001 and 21472002） and Natural Science Foundation of Anhui Province （Grant No. 1508085J07）.
文摘Multichromophoric boron dipyrromethene （BODIPY） dyes have been efficiently synthesized from a one-pot condensation of acyl chloride with 2,4-dimethylpyrrole and have been converted to energy transfer cassettes through a one-pot Knoevenagel condensation. Various BODIPYs containing 2-ketopyrrole unit were also isolated as the side products and one of their BF2 complex was also isolated from these condensation reactions. The fluores- cence quantum yields of these phenylene bridged dimers and trimer are much lower than those of their correspond- ing monomers. A significant decrease of the fluorescence quantum yield was observed in most polar solvents for these dimers and trimer, which may be due to a possible symmetry breaking resulted intramolecular charge transfer （ICT） in the excited state. Efficient energy transfer between the donor and acceptor was observed in these energy transfer cassettes, which could be useful as large pseudo-Stokes shift fluorescent dyes with potential applications in diverse fields.
