Receptor-like cytoplasmic kinases(RLCKs),which belong to a large subgroup of receptor-like kinases in plants,play crucial roles in plant development and immunity.However,their functions and regulatory mechanisms in pl...Receptor-like cytoplasmic kinases(RLCKs),which belong to a large subgroup of receptor-like kinases in plants,play crucial roles in plant development and immunity.However,their functions and regulatory mechanisms in plants remain unclear.Here,we report functional characterization of OsRLCK118 from the OsRLCK34 subgroup in rice(Oryza sativa L.).Expression of OsRLCK118 could be induced by infections with Xanthomonas oryzae pv.oryzae(Xoo)strains PXO68 and PXO99.Silencing of OsRLCK118 altered plant height,flag-leaf angle and second-topleaf angle.Silencing of OsRLCK118 also resulted in increasing susceptibility to Xoo and Magnaporthe oryzae(M.oryzae)in rice plants.OsRLCK118 knock-out plants were more sensitive to bacterial blight whereas OsRLCK118 overexpressor plants exhibited increased disease resistance.Expression levels of pathogenesis-related genes of OsPAL1,OsNH1,OsICS1,OsPR1a,OsPR5 and OsPR10 were reduced in the rlck118 mutant compared to wild-type rice(Dongjin)and knock-out of OsRLCK118 compromised the production of reactive oxygen species.These results suggest that OsRLCK118 may modulate basal resistance to Xoo and M.oryzae,possibly through regulation of ROS burst and hormone mediated defense signaling pathway.展开更多
基金supported by the National Natural Science Foundation(31860497)Natural Science Foundation of Hainan Province(No.2019RC013)and Hainan Provincial Department of Education[Hnjg2019ZD-2].
文摘Receptor-like cytoplasmic kinases(RLCKs),which belong to a large subgroup of receptor-like kinases in plants,play crucial roles in plant development and immunity.However,their functions and regulatory mechanisms in plants remain unclear.Here,we report functional characterization of OsRLCK118 from the OsRLCK34 subgroup in rice(Oryza sativa L.).Expression of OsRLCK118 could be induced by infections with Xanthomonas oryzae pv.oryzae(Xoo)strains PXO68 and PXO99.Silencing of OsRLCK118 altered plant height,flag-leaf angle and second-topleaf angle.Silencing of OsRLCK118 also resulted in increasing susceptibility to Xoo and Magnaporthe oryzae(M.oryzae)in rice plants.OsRLCK118 knock-out plants were more sensitive to bacterial blight whereas OsRLCK118 overexpressor plants exhibited increased disease resistance.Expression levels of pathogenesis-related genes of OsPAL1,OsNH1,OsICS1,OsPR1a,OsPR5 and OsPR10 were reduced in the rlck118 mutant compared to wild-type rice(Dongjin)and knock-out of OsRLCK118 compromised the production of reactive oxygen species.These results suggest that OsRLCK118 may modulate basal resistance to Xoo and M.oryzae,possibly through regulation of ROS burst and hormone mediated defense signaling pathway.
