We report a novel nanotechnology-based approach for the highly efficient catalytic oxidation of phenols and their removal from wastewater.We use a nanocomplex made of multi-walled carbon nanotubes(MWNTs)and magnetic n...We report a novel nanotechnology-based approach for the highly efficient catalytic oxidation of phenols and their removal from wastewater.We use a nanocomplex made of multi-walled carbon nanotubes(MWNTs)and magnetic nanoparticles(MNPs).This nanocomplex retains the magnetic properties of individual MNPs and can be effectively separated under an external magnetic fi eld.More importantly,the formation of the nanocomplex enhances the intrinsic peroxidase-like activity of the MNPs that can catalyze the reduction of hydrogen peroxide(H2O2).Significantly,in the presence of H2O2,this nanocomplex catalyzes the oxidation of phenols with high effi ciency,generating insoluble polyaromatic products that can be readily separated from water.展开更多
We recognize the stochastic collisions of dopamine contained phospholipid vesicle on carbon fiber nanoelectrode, extending the observation of discrete collision events on nanoelectrode to biologically relevant analyte...We recognize the stochastic collisions of dopamine contained phospholipid vesicle on carbon fiber nanoelectrode, extending the observation of discrete collision events on nanoelectrode to biologically relevant analytes. To decrease noise interference to the technique, the dimensions of nanoelectrode was systematically investigated and optimized. Scanning electron microscopy(SEM) further supported the comparable sizes of nanoelectrode and vesicles(~100 nm in diameter). Vesicles collision and rupture on the surface of nanoelectrode led to the dopamine release from vesicles, which could be electrochemically oxidized to dopamine-o-quinone and detected via voltammetry. The comparable size of the nanoelectrode with vesicles and fast voltammetry allowed differentiation of single collision events from the current magnitudes and peak widths in the electrochemical collision experiments, which shows the efficacy of the method to characterize vesicle samples. This work provides a foundation upon which quantitative sensor technology might be built for the detection of dopamine contained vesicles with high spatial and temporal resolution.展开更多
Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nuc...Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided.展开更多
Self-assembled DNA nanostructures have shown remarkable potential in the engineering of biosensing interfaces,which can improve the performance of various biosensors.In particular,by exploiting the structural rigidity...Self-assembled DNA nanostructures have shown remarkable potential in the engineering of biosensing interfaces,which can improve the performance of various biosensors.In particular,by exploiting the structural rigidity and programmability of the framework nucleic acids with high precision,molecular recognition on the electrochemical biosensing interface has been significantly enhanced,leading to the development of highly sensitive and specific biosensors for nucleic acids,small molecules,proteins,and cells.In this review,we summarize recent advances in DNA framework-engineered biosensing interfaces and the application of corresponding electrochemical biosensors.展开更多
Single entities detection reveals heterogeneity and random processes hidden in ensemble measurements.Obtaining accurate single-entity information is challenging.The electrochemical analysis is at high spatial resoluti...Single entities detection reveals heterogeneity and random processes hidden in ensemble measurements.Obtaining accurate single-entity information is challenging.The electrochemical analysis is at high spatial resolution and high temporal resolution to analyze single entities and measure the fast kinetics process.In this minireview,we will focus on the electrochemical strategies for single multiscale entities.展开更多
Cellular uptake of biomolecules is crucial for regulating cell function.However,powerful and biocompatible tools for dynamically manipulating the cell entry of single-stranded DNAs(ssDNAs)remain elusive.Herein,we cons...Cellular uptake of biomolecules is crucial for regulating cell function.However,powerful and biocompatible tools for dynamically manipulating the cell entry of single-stranded DNAs(ssDNAs)remain elusive.Herein,we constructed synthetic DNA circuits on the cell membrane to program the cell entry of ssDNAs,using toehold-mediated DNA strand displacement reactions.We found that the dimerization and trimerization of cholesterol-ssDNAs enhanced membrane-anchoring and cellular uptake of ssDNAs.Moreover,we demonstrated that de-dimerization and de-trimerization of cholesterol-ssDNAs could be accomplished by inputting recovery ssDNAs into the synthetic DNA circuits,which could simultaneously decrease the cellular uptake of ssDNAs.We speculate that operating the synthetic DNA circuits on the cell membrane will be a powerful strategy for regulating the cellular uptake of exogenous materials,which has important implications for bioimaging,drug delivery,and gene therapy.展开更多
The outbreak of infectious diseases often arouses public health emergencies of international concern.During the past several decades,we have witnessed the outbreaks of various epidemics and pandemics,such as AIDS,Ebol...The outbreak of infectious diseases often arouses public health emergencies of international concern.During the past several decades,we have witnessed the outbreaks of various epidemics and pandemics,such as AIDS,Ebola,SARS,dengue,Zika,bird flu,and particularly the recent coronavirus disease 2019(COVID-19)caused by SARSCoV-2.Bioassays have been generally regarded as the first defense for the prevention and control of infectious diseases.Given the representative course of these diseases,bioassays against certain biomarkers are suitable for distinct stages of infection in diverse scenarios(Figure 1).However,the deployed bioassays still possess inherent limitations.Here,we briefly summarize the pros and cons of state-of-the-art bioassays for infectious diseases caused by pathogenic viruses and provide an outlook on the advances in the context of public health emergencies.展开更多
基金the National Natural Science Foundation(20873175 and 20725516)the Shanghai Municipal Commission for Science and Technology(0752nm021)the Ministry of Science and Technology of China(2006CB933000,2007CB936000,and 2007AA06A406).
文摘We report a novel nanotechnology-based approach for the highly efficient catalytic oxidation of phenols and their removal from wastewater.We use a nanocomplex made of multi-walled carbon nanotubes(MWNTs)and magnetic nanoparticles(MNPs).This nanocomplex retains the magnetic properties of individual MNPs and can be effectively separated under an external magnetic fi eld.More importantly,the formation of the nanocomplex enhances the intrinsic peroxidase-like activity of the MNPs that can catalyze the reduction of hydrogen peroxide(H2O2).Significantly,in the presence of H2O2,this nanocomplex catalyzes the oxidation of phenols with high effi ciency,generating insoluble polyaromatic products that can be readily separated from water.
基金supported by the National Natural Science Foundation of China(21422508,31470960)Chinese Academy of Sciencessupport by the Deanship of Scientific Research,College of Science Research Center at King Saud University
文摘We recognize the stochastic collisions of dopamine contained phospholipid vesicle on carbon fiber nanoelectrode, extending the observation of discrete collision events on nanoelectrode to biologically relevant analytes. To decrease noise interference to the technique, the dimensions of nanoelectrode was systematically investigated and optimized. Scanning electron microscopy(SEM) further supported the comparable sizes of nanoelectrode and vesicles(~100 nm in diameter). Vesicles collision and rupture on the surface of nanoelectrode led to the dopamine release from vesicles, which could be electrochemically oxidized to dopamine-o-quinone and detected via voltammetry. The comparable size of the nanoelectrode with vesicles and fast voltammetry allowed differentiation of single collision events from the current magnitudes and peak widths in the electrochemical collision experiments, which shows the efficacy of the method to characterize vesicle samples. This work provides a foundation upon which quantitative sensor technology might be built for the detection of dopamine contained vesicles with high spatial and temporal resolution.
文摘Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided.
基金supported by the National Natural Science Foundation of China(21804091,21804088,21904086)Shanghai Pujiang Program(19PJ1407300)。
文摘Self-assembled DNA nanostructures have shown remarkable potential in the engineering of biosensing interfaces,which can improve the performance of various biosensors.In particular,by exploiting the structural rigidity and programmability of the framework nucleic acids with high precision,molecular recognition on the electrochemical biosensing interface has been significantly enhanced,leading to the development of highly sensitive and specific biosensors for nucleic acids,small molecules,proteins,and cells.In this review,we summarize recent advances in DNA framework-engineered biosensing interfaces and the application of corresponding electrochemical biosensors.
基金the National Natural Science Foundation of China(21804091,21804089)Shanghai Pujiang Program(19PJ1407300).
文摘Single entities detection reveals heterogeneity and random processes hidden in ensemble measurements.Obtaining accurate single-entity information is challenging.The electrochemical analysis is at high spatial resolution and high temporal resolution to analyze single entities and measure the fast kinetics process.In this minireview,we will focus on the electrochemical strategies for single multiscale entities.
基金supported by the National Natural Science Foundation of China(Grants No.22025404,21904086).
文摘Cellular uptake of biomolecules is crucial for regulating cell function.However,powerful and biocompatible tools for dynamically manipulating the cell entry of single-stranded DNAs(ssDNAs)remain elusive.Herein,we constructed synthetic DNA circuits on the cell membrane to program the cell entry of ssDNAs,using toehold-mediated DNA strand displacement reactions.We found that the dimerization and trimerization of cholesterol-ssDNAs enhanced membrane-anchoring and cellular uptake of ssDNAs.Moreover,we demonstrated that de-dimerization and de-trimerization of cholesterol-ssDNAs could be accomplished by inputting recovery ssDNAs into the synthetic DNA circuits,which could simultaneously decrease the cellular uptake of ssDNAs.We speculate that operating the synthetic DNA circuits on the cell membrane will be a powerful strategy for regulating the cellular uptake of exogenous materials,which has important implications for bioimaging,drug delivery,and gene therapy.
基金supported by the National Natural Science Foundation of China(22074093)Science Foundation of the Shanghai Municipal Science and Technology Commission(21dz2210100)Shanghai Yangfan Program(21YF1459500).
文摘The outbreak of infectious diseases often arouses public health emergencies of international concern.During the past several decades,we have witnessed the outbreaks of various epidemics and pandemics,such as AIDS,Ebola,SARS,dengue,Zika,bird flu,and particularly the recent coronavirus disease 2019(COVID-19)caused by SARSCoV-2.Bioassays have been generally regarded as the first defense for the prevention and control of infectious diseases.Given the representative course of these diseases,bioassays against certain biomarkers are suitable for distinct stages of infection in diverse scenarios(Figure 1).However,the deployed bioassays still possess inherent limitations.Here,we briefly summarize the pros and cons of state-of-the-art bioassays for infectious diseases caused by pathogenic viruses and provide an outlook on the advances in the context of public health emergencies.
