Grain weight is one of the key components of wheat(Triticum aestivum L.)yield.Genetic manipulation of grain weight is an efficient approach for improving yield potential in breeding programs.A recombinant inbred line(...Grain weight is one of the key components of wheat(Triticum aestivum L.)yield.Genetic manipulation of grain weight is an efficient approach for improving yield potential in breeding programs.A recombinant inbred line(RIL)population derived from a cross between W7268 and Chuanyu 12(CY12)was employed to detect quantitative trait loci(QTLs)for thousand-grain weight(TGW),grain length(GL),grain width(GW),and the ratio of grain length to width(GLW)in six environments.Seven major QTLs,QGl.cib-2D,QGw.cib-2D,QGw.cib-3B,QGw.cib-4B.1,QGlw.cib-2D.1,QTgw.cib-2D.1 and QTgw.cib-3B.1,were consistently identified in at least four environments and the best linear unbiased estimation(BLUE)datasets,and they explained 2.61 to 34.85%of the phenotypic variance.Significant interactions were detected between the two major TGW QTLs and three major GW loci.In addition,QTgw.cib-3B.1 and QGw.cib-3B were co-located,and the improved TGW at this locus was contributed by GW.Unlike other loci,QTgw.cib-3B.1/QGw.cib-3B had no effect on grain number per spike(GNS).They were further validated in advanced lines using Kompetitive Allele Specific PCR(KASP)markers,and a comparison analysis indicated that QTgw.cib-3B.1/QGw.cib-3B is likely a novel locus.Six haplotypes were identified in the region of this QTL and their distribution frequencies varied between the landraces and cultivars.According to gene annotation,spatial expression patterns,ortholog analysis and sequence variation,the candidate gene of QTgw.cib-3B.1/QGw.cib-3B was predicted.Collectively,the major QTLs and KASP markers reported here provide valuable information for elucidating the genetic architecture of grain weight and for molecular marker-assisted breeding in grain yield improvement.展开更多
Grain size and weight are key components of wheat yield.Exploitation of major underlying quantitative trait loci(QTL)can improve yield potential in wheat breeding.A recombinant inbred line(RIL)population was construct...Grain size and weight are key components of wheat yield.Exploitation of major underlying quantitative trait loci(QTL)can improve yield potential in wheat breeding.A recombinant inbred line(RIL)population was constructed to detect QTL for thousand-grain weight(TGW),grain length(GL)and grain width(GW)across eight environments.Genomic regions associated with grain size and grain weight were identified on chromosomes 4A and 6A using bulked segregant exome sequencing(BSE-Seq)analysis.After constructing genetic maps,six major QTL detected in at least four individual environments and in best linear unbiased estimator(BLUE)datasets,explained 7.50%-23.45%of the phenotypic variation.Except for QGl.cib-4A,the other five QTL were co-located in two regions,namely QTgw/Gw.cib-4A and QTgw/Gw/Gl.cib-6A.Interactions of these QTL were analyzed.Unlike QTgw/Gw/Gl.cib-6A,QTgw/Gw.cib-4A and QGl.cib-4A had no effect on grain number per spike(GNS).The QTL were validated in a second cross using Kompetitive Allele Specific PCR(KASP)markers.Since QTgw/Gw.cib-4A was probably a novel locus,it and the KASP markers reported here can be used in wheat breeding.TraesCS4A03G0191200 was predicted to be potential candidate gene for QTgw/Gw.cib-4A based on the sequence differences,spatiotemporal expression patterns,gene annotation and haplotype analysis.Our findings will be useful for fine mapping and for marker-assisted selection in wheat grain yield improvement.展开更多
Advertisement calls play an important role in influencing female mate choice and are regarded as one of the key characters responsible for reproductive isolation and speciation events in frogs and toads. The black-spi...Advertisement calls play an important role in influencing female mate choice and are regarded as one of the key characters responsible for reproductive isolation and speciation events in frogs and toads. The black-spined toad, Bufo melanostictus, is one of the most common amphibians in Southeast Asia and is frequently observed in human habitations throughout its distributional range. The advertisement calls of the toad have not been well described. Here, we report the advertisement calls of this species from Lishui, Zhejiang, China during its breeding season. The advertisement calls of B. melanostictus are composed of single notes with a single harmonic frequency emitted sporadically. The call parameters recorded in this study were highly variable across recording times and with different body sizes. Dominant frequency was negatively correlated with body size. Comparing the calls of the B. melanostictus population from Lishui with the populations from five other localities(Bangkok, Thailand; Coorg, India; Burma, Myanmar; Bali, Indonesia; Halimun-Salak National Park, Indonesia), we found that call structure was similar among five different populations with no harmonics, while the Halimun-Salak National Park population contained two clear harmonics. Dominant frequency was similar between Bali(1.56 kHz) and Coorg(1.6 kHz) populations, while it was highly variable among other populations. Besides call duration, call interval and pulse/call rate may also be significantly different among the comparing populations. Pooling the available data, we observed some similarities and differences in call parameters among the six populations.展开更多
A data identifier(DID)is an essential tag or label in all kinds of databases—particularly those related to integrated computational materials engineering(ICME),inheritable integrated intelligent manufacturing(I3M),an...A data identifier(DID)is an essential tag or label in all kinds of databases—particularly those related to integrated computational materials engineering(ICME),inheritable integrated intelligent manufacturing(I3M),and the Industrial Internet ofThings.With the guidance and quick acceleration of the developme nt of advanced materials,as envisioned by official documents worldwide,more investigations are required to construct relative numerical standards for material informatics.This work proposes a universal DID format consisting of a set of build chains,which aligns with the classical form of identifier in both international and national standards,such as ISO/IEC 29168-1:2000,GB/T 27766-2011,GA/T 543.2-2011,GM/T 0006-2012,GJB 7365-2011,SL 325-2014,SL 607-201&WS 363.2-2011,and QX/T 39-2005.Each build chain is made up of capital letters and numbers,with no symbols.Moreover,the total length of each build chain is not restricted,which follows the formation of the Universal Coded Character Set in the international standard of ISO/IEC 10646.Based on these rules,the proposed DID is flexible and convenient for extendi ng and sharing in and between various cloud-based platforms.Accordingly,classical two-dimensional(2D)codes,including the Hanxin Code,Lots Perception Matrix(LP)Code,Quick Response(Q.R)code,Grid Matrix(GM)code,and Data Matrix(DM)Code,can be constructed and precisely recognized and/or decoded by either smart phones or specific machines.By utilizing these 2D codes as the fingerprints of a set of data linked with cloud-based platforms,progress and updates in the composition-processing-structure-property-performance workflow process can be tracked spontaneously,paving a path to accelerate the discovery and manufacture of advanced materials and enhance research productivity,performance,and collaboration.展开更多
Alien species are one of the most serious threats to the decline and extinction of native amphibian populations. In this study, we examined the predation of invasive Western Mosquitofish Gambusia affinis on the eggs, ...Alien species are one of the most serious threats to the decline and extinction of native amphibian populations. In this study, we examined the predation of invasive Western Mosquitofish Gambusia affinis on the eggs, embryos, and tadpoles of Duttaphrynus melanostictus and Pelophylax nigromaculatus in south China. Our results suggested that the survival of eggs and embryos remaining in the egg capsules of P. nigromaculatus and D. melanostictus was significantly higher than those removed from the egg capsule at 12-h intervals within 72 h in the presence of G. affinis. The survival of P. nigromaculatus eggs and embryos without egg capsules was significantly lower than those of D. melanostictus without egg capsules. The survival of P. nigromaculatus eggs and embryos with egg capsules was significantly higher than those of D. melanostictus with egg capsules from 24 h to 72 h except for 12 h. The survival of D. melanostictus tadpoles was significantly higher than that of P. nigromaculatus tadpoles in the presence of G. affinis. The survival of Gosner stage 26 tadpoles of P. nigromaculatus was significantly higher than that of Gosner stage 30 tadpoles from 12 h to 60 h, but there were no significant differences at 72 h. In contrast, the survival of Gosner stage 26 tadpoles of D. melanostictus was significantly lower than that of Gosner stage 30 tadpoles within 72 h, recording every 12 h. The increasing temperature caused a significant increase in predation by G. affinis on P. nigromaculatus eggs and embryos. The outer jelly capsule surrounding anurans eggs might serve as a mechanical defense against predation by G. affinis due to its large diameter, relatively stationary state and unpalatability. The differences in the vulnerability of P. nigromaculatus and D. melanostictus embryos and tadpoles to G. affinis probably due to differences in the unpalatability, black skin and activity. Based on the magnitude of predation by G. affinis on the eggs, embryos and tadpoles of these two species and the combined impact of temperature, we might speculate that invasive G. affinis and global warming would have more detrimental impacts on population viability of P. nigromaculatus than D. melanostictus in China.展开更多
Kinship and density are believed to affect important ecological processes such as intraspecific competition, predation, growth, development, cannibalism, habitat selection and mate choice, In this work, we used Chines...Kinship and density are believed to affect important ecological processes such as intraspecific competition, predation, growth, development, cannibalism, habitat selection and mate choice, In this work, we used Chinese tiger frog Hoplobatrachus chinensis tadpoles as an experimental model to investigate the effects of kinship and density on growth and development of this species over a 73 day period. The results showed that density can affect the growth and developmental traits (survival rate, larval period, size at the limb bud protrusion/metamorphic climax and body mass at different life stages) of H. chinensis tadpoles, while kinship does not. Tadpoles took longer to develop and potential metamorphosis was greater in high density groups of both sibling and non-siblings. The interaction of kinship and density did not significantly influenced growth traits of H. chinensis tadpoles during the experimental period. For coefficient variations of each growth trait, no differences were detected between sibling and non-sibling groups. These findings provide valuable information on the basic ecology of H. chinensis which will be helpful in future studies of other anuran species.展开更多
Lung cancer is one of the most important causes of cancer-related mortality worldwide. Human cytochrome P450 2A13 enzyme (CYP2A13) is predominantly expressed in the respiratory tract and could catalyze various carci...Lung cancer is one of the most important causes of cancer-related mortality worldwide. Human cytochrome P450 2A13 enzyme (CYP2A13) is predominantly expressed in the respiratory tract and could catalyze various carcinogens. In this study, we quantified CYP2A13 expression in non-small cell lung cancer (NSCLC) tissues and examined the relation between CYP2A13 and clinicopathologic factors. Thirty-five paired lung cancer and normal tissues were studied for the expression of the CYP2A13 gene by using real-time PCR and Western blot- ting assays. We also investigated the relationship between CYP2A13 expression and clinicopathologic factors such as age, gender, histology and lymph node status in tumor tissues. SPSS (17.0) statistical software was applied for data analysis. The real-time PCR results showed that there was no significant difference in the CYP2A13 mRNA transcript levels between tumor and paired normal tissues in the 35 samples and in 12 paired squamous cell car- cinomas. In adenocarcinoma, the expression of CYP2A13 mRNA in tumor tissues was 12.5% of that in adjacent tissues (P 〈 0.05) and it was not associated with age, gender, histology and lymph node status of the patients. The amounts of CYP2A13 proteins detected by Western blotting assays correlated well with those of the correspond- ing mRNAs. In conclusion, the expression of CYP2A13 was downregulated in lung adenocarcinoma. CYP2A13 may be involved in the development and progression of lung adenocarcinoma.展开更多
BACKGROUND: Expression of Fos in neurons of periaqueductal gray (PAG) is used to reflect the excitability. However, changes of expression of Fos in neurons of PAG are caused by injured electrostimulation after simu...BACKGROUND: Expression of Fos in neurons of periaqueductal gray (PAG) is used to reflect the excitability. However, changes of expression of Fos in neurons of PAG are caused by injured electrostimulation after simulated weightlessness, and the relationship between pretreatment and injection of succinylcholine has not been determined yet. OBJECTIVE : To investigate the changes of expression of Fos in PAG induced by injured electrostimulation pretreatment and injection of succinylcholine at 2 weeks after simulated weightlessness.DESIGN: Observational and controlled animal study.SETTING: Department of Physiology, Medical School, Xi'an Jiaotong University; Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education. MATERIALS: A total of 24 adult female SD rats, of clean grade and weighing 180-220 g, were selected in this study. METHODS: The experiment was completed in the Experimental Animal Center of Xi'an Jiaotong University.① All rats were randomly divided into 2 groups according to body mass: simulated weightlessness group and control group with 12 in each group. And then, each group was also divided into 3 subgroups: electrostimulation group, succinylcholine-pretreatment group and succinylcholine-injection group with 4 in each subgroup. ②The model of weightlessness was simulated by tail-suspended female rats, which were described and modified by Cheng Jie. Rats in normal control group were given the same interventions as simulated weightlessness group except for tail-suspended. ③ Experimental method: The rats in electrostimulation group were given nociceptive stimulus by a pair of subcutaneous electrodes inserted into 1 and 5 claw of left hindlimb. The stimulus (current: 10 mA; duration: 1 ms; interval: 1 s) lasted for 30 minutes. The rats in succinylcholine-pretreatment group received stimulus after intravenous administration of succinylcholine, rats in succinylcholine-injection group were not given stimulus, just received succinylcholine. ④ All rats were perfused and fixed after 2 hours from the end of stimulation. The brains were removed, and serial frozen sections of midbrain were stained using immunocytochemical method, observed and taken photos under light-microscope. The number and morphological characters of Fos-immunoreactive (Fos-IR) neurons in ventrolateral part of PAG were investigated. MAIN OUTCOME MEASURES: The alterations in number and morphological characters of Fos-IR neurons in ventrolateral PAG of all rats.RESULTS: A total of 24 rats were involved in the final analysis. ① The morphological changes of Fos-IR neurons: The expressions of Fos in ventrolateral part of PAG were observed in both control and simulated weightlessness groups rats after being given nociceptive stimulus. As compared with control group, Fos-IR neurons in simulated weightlessness group were dyed lightly, cellular integrity was impaired, and cellular verge was unclear. ② The numbers of Fos-IR neurons: In control group, the numbers of Fos-IR neurons in ventrolateral part of PAG in simulated weightlessness group were obviously lower than succinylcholine-pretreatment group, but obviously higher than succinylcholine-injection group (46.94±3.38, 71.06±8.96 and 35.04±4.62, respectively, P 〈 0.05). In 14-day simulated weightlessness group, the numbers of Fos-IR neurons in electrostimulation group were also obviously lower than succinylcholine-pretreatment group, and obviously higher than succinylcholine-injection group (27.77±3.27, 32.91±2.99 and 11.75±1.00, respectively, P 〈 0.05). The numbers of Fos-IR neurons in all subgroups in control group were obviously higher than those subgroups in simulated weightlessness group. Compared with electrostimulation group, the percentage of expression of Fos in ventrolateral part of PAG responsed to nociceptive stimulus after administration of succinylcholine (SCH) was increased to 51.83% in control group and 18.51% in simulated weightlessness group.CONCLUSION :① The expression of Fos in neurons in ventrolateral part of PAG were increased by the pretreatment of SCH before nociceptive stimulus.② Nociceptive stimulus could increase the expression of Fos in neurons in ventrolateral part of PAG. ③ The numbers of Fos-IR neurons in ventrolateral part of PAG were decreased obviously after 2-week simulated weightlessness.展开更多
BACKGROUND: Prenatal stress has been shown to inhibit cell proliferation in the dentate gyrus and hippocampus, reduce hippocampal volume, and cause neuronal loss and oxidative damage in the hippocampus of offspring r...BACKGROUND: Prenatal stress has been shown to inhibit cell proliferation in the dentate gyrus and hippocampus, reduce hippocampal volume, and cause neuronal loss and oxidative damage in the hippocampus of offspring rats, but the sexual difference of the effects on offsprings is seldom referred to. OBJECTIVE: To observe the effect of prenatal stress to adult pregnant rats on expression of extracellular signal-regulated kinases (ERK) in hippocampus of the offspring rats of different genders. DESIGN : A randomized and control animal experiment.SETTING: Department of Physiology and Pathophysiology, School of Medicine, Xi'an Jiaotong University. MATERIALS : The experiments were carried out in the Key Laboratory of Environment and Gene Related Diseases (Xi'an Jiaotong University), Ministry of Education between October 2005 and March 2006. Fifteen female and five male adult Sprague-Dawley rats were adopted. Female rats weighing 230-250 g and male rats weighing 280-350 g were used. METHODS: The virgin female rats were placed overnight with adult male rats (3:1) for mating. A total of twelve pregnant rats were randomly assigned to prenatal stress group (PNS group, n=6) and control group (n=6). The pregnant rats of the PNS group were exposed to restraint stress on days 14-20 of pregnancy three times a day, 45 minutes for each time . The restraint device was a transparent plastic tube (6.8 cm in diameter) with air holes for breathing and closed end. The length could be adjusted to accommodate the size of the animals. To prevent habituation of animals to the daily procedure, restraint periods were randomly shifted within certain time periods (8:00-11:00, 11:00-14:00, and 16:00-19:00). After birth, offsprings of all groups were culled to 8-10 litters in each group and housed in the same animal room, and kept together with their biologic mothers. The pregnant rats of the control group were left undisturbed. On day 21, after all the offspring were weaned, male and female pups were separated and housed four in each cage respectively until test at 30 days of age. At the end of postnatal day 30, one male and female offspring rats from the same dam were selected with a random choice and a total of 24 animals from 12 different dams were used. The experimental rats were sacrificed by decapitation under anesthesia. Bilateral hippocampal tissues were isolated and homogenized in cold condition. Alkaline carbonate buffer (BCA) method was used to detect the concentration of extracellular signal-regulated kinases (ERK), then mixed with loading buffer, the constant voltage was 100 V. Finally, BCIP/NBT staining and electrDphoresis were performed, the absorbance (A) value for the bands was detected with the Bandscan analytical software, and the expression of ERK in hippocampus of offspring rats of different genders in each group was quantitatively analyzed. MAIN OUTCOME MEASURES: The level of ERK expression in hippocampus of offspring rats of different genders in each group was observed.RESULTS: All the 24 offspring rats were involved in the analysis of results. ① The staining results of ERP activity in the extract of brain tissue detected with Western blotting technique and specific antibody analysis showed that the ERP in hippocampus of offspring rats had two subtypes of ERK-1 and ERK-2, and the latter was the main type.② Standardized by the average A value in the control group, the quantitative data of the general A value of total ERK showed that the expression of ERK-2 in hippocampus of female offspring rats was obviously higher in the PNS group than in the control group (A value: 126±6.76,100±4.89,P〈 0.01). ③The expression of ERK-2 had no obvious difference between the female and male offspring rats in the control group.④ The expression of ERK-2 in hippocampus of male offspring rats was a little higher in the PNS group than in the control group (A value: 104±6.27,102±5.48,P 〉 0.05). CONCLUSION : PNS significantly affects the increase of ERK expression in hippocampus of female offspring rats, but has no obvious influence on that of male ones.展开更多
Bisphenol A dicyanate ester resins modified by fluorine-containing liquid crystal compound(LCFE)are applied as polymer matrix(LCFE-BADCy),poly(p-phenylene-2,6-benzobisoxazole)(PBO)fibers as rein-forcements,and fluorin...Bisphenol A dicyanate ester resins modified by fluorine-containing liquid crystal compound(LCFE)are applied as polymer matrix(LCFE-BADCy),poly(p-phenylene-2,6-benzobisoxazole)(PBO)fibers as rein-forcements,and fluorine/adamantane PBO precursor(pre FABPBO)as interfacial compatibilizer to prepare the corresponding PBO fibers/FABPBO/LCFE-BADCy wave-transparent laminated composites.LCFE could improve the order degree of BADCy cured network,in favor of enhancing the wave-transparent perfor-mance,mechanical properties,and intrinsic thermal conductivity.The dielectric constant and dielectric loss of PBO fibers/FABPBO/LCFE-BADCy composites are highly temperature(25–200℃)and frequency(10^(4)–10^(7) Hz and 8.2–12.4 GHz)stable with the value of 2.49 and 0.003 under 10^(6) Hz at 25℃,and the corresponding wave transmission efficiency is 95.0%,higher than that of 92.5%for PBO fibers/BADCy com-posites.The interlamellar shear strength and flexural strength are respectively 50.7 MPa and 682.5 MPa,38.1%and 16.2%higher than those of PBO fibers/BADCy composites.Besides,the volume resistivity,breakdown voltage,heat resistance index,glass transition temperature,flame retardant grade,and ul-timate oxygen index of PBO fibers/FABPBO/LCFE-BADCy composites are respectively 5.3×10^(15)Ωcm,29.75 kV/mm,217.2℃,245.7℃,V-1 grade,and 33.6%,expected to be performed as a new generation of“lightweight/loading/wave-transparent”electromagnetic window materials in advanced military weapons and civil communication base station.展开更多
Comprehensive Summary Branched fluorine/adamantane PBO precursor(preFABPBO),synthesized via random co-condensation between 2,2-bis(3-amino-4-hydroxyphenyl)hexafluoro propane,1,3-adamantanedicarbonyl dichloride,and tri...Comprehensive Summary Branched fluorine/adamantane PBO precursor(preFABPBO),synthesized via random co-condensation between 2,2-bis(3-amino-4-hydroxyphenyl)hexafluoro propane,1,3-adamantanedicarbonyl dichloride,and trimesoyl chloride,is performed as interfacial compatibilizer,bisphenol A dicyanate ester(BADCy)resin as polymer matrix,and poly(p-phenylene-2,6-benzobisoxazole)(PBO)fibers as reinforcements to prepare PBO fibers/FABPBO-BADCy wave-transparent laminated composites by high temperature molding.The mechanical properties,wave-transparent performances,and heat resistances of PBO fibers/BADCy composites are simultaneously improved by the addition of preFABPBO.The interlaminar shear strength(ILSS)and flexural strength of PBO fibers/FABPBO-BADCy composites are 48.9 and 665.3 MPa,respectively,increased by 33.2%and 13.3%compared to those of PBO fibers/BADCy composites(36.7 and 587.4 MPa).The dielectric constant and dielectric loss values at 106 Hz are 2.53 and 0.003,respectively,lower than those of PBO fibers/BADCy composites(3.06 and 0.006),and the corresponding wave transmission efficiency is 94.8%,which also presents excellent stability over the wide temperature(25-200℃)and frequency range(103-107 Hz and 8.2-12.4 GHz).Meanwhile,the heat resistance index and glass transition temperature are 229.9℃and 247.5℃,also better than those of PBO fibers/BADCy composites(229.6℃and 247.1℃).展开更多
To the Editor:Autoimmune liver disease is a chronic immune-mediated liver disease.The term“overlap syndrome”(OS)is used to describe the conditions in which patients simultaneously or consecutively present with featu...To the Editor:Autoimmune liver disease is a chronic immune-mediated liver disease.The term“overlap syndrome”(OS)is used to describe the conditions in which patients simultaneously or consecutively present with features of primary biliary cholangitis(PBC)or primary sclerosing cholangitis and autoimmune hepatitis(AIH).[1]PBC-AIH OS is described most frequently,[2]with a prevalence of approximately 8%to 20%in adult patients with either pure PBC or AIH.展开更多
Metal-organic frameworks(MOFs)and covalent organic frameworks(COFs)as the novel porous materials have the merits of diverse,adjustable functionality,high porosity and surface area,which have great application prospect...Metal-organic frameworks(MOFs)and covalent organic frameworks(COFs)as the novel porous materials have the merits of diverse,adjustable functionality,high porosity and surface area,which have great application prospects in the gas storage,separation and catalysis.In addition,their derivates make up for the insufficient of electronic conductivity and chemical stability of MOFs and COFs,and provide a new ideal for accurate control of material structure.Up to now,many efficient electrocatalysts have been designed based on MOFs,COFs and their derivates for O_(2)reduction/evolution reactions(ORR/OER)and CO_(2)reduction/evolution reactions(CO_(2)RR/CO_(2)ER)in the metal-air batteries.In this review,the latest development of MOFs,COFs and their derivates in the metal-air batteries is summarized,and we discuss the structural characteristics of these materials and their corresponding mechanisms of action.By comprehensively reviewing the advantages,challenges and prospects of MOFs and COFs,we hope that the organic framework materials will shed more profound insights into the development of electrocatalysis and energy storage in the future.展开更多
Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for th...Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait.展开更多
Metal-organic framework materials(MOFs)have been widely stu-died because of their adjustable composition and controllable structure in the field of microwave absorption(MA).Therein,Prussian blue analogs(PBA)have attra...Metal-organic framework materials(MOFs)have been widely stu-died because of their adjustable composition and controllable structure in the field of microwave absorption(MA).Therein,Prussian blue analogs(PBA)have attracted the attention of researchers with ultra-high metal content.However,the attenua-tion ability of microwave for PBA-based composites is still unsatis-factory up to now.Therefore,the NiFe/CoFe@C composites were prepared by carbonizing polymetallic PBA(NiCoFe PBA)materials in this work,and the influence of different metal alloy components on MA was explored by adjusting the ratio of metal ions(Ni^(2+)/Co^(2+)).Moreover,the NiFe/CoFe@C composites have rich interfaces and enhance the polarization loss due to the introduction of Ni and it has an optimal performance at 2.7 mm that is the reflection loss(RL)is−41.49 dB and an effective absorption bandwidth(EAB)is 7.12 GHz with 1/1(Ni^(2+)/Co^(2+)).The above data provides a research idea for obtaining light and efficient absorbers.展开更多
Hybrid polymer membrane(TA-APTES),synthesized by tannic acid(TA)and aminopropyl trethoxysilane(APTES)based on the Schif’s base and Michael addition reaction,is deposited on the surface of poly(p-phenylene-2,6-benzobi...Hybrid polymer membrane(TA-APTES),synthesized by tannic acid(TA)and aminopropyl trethoxysilane(APTES)based on the Schif’s base and Michael addition reaction,is deposited on the surface of poly(p-phenylene-2,6-benzobisoxazole)(PBO)fbers,and then grafted with epoxy-terminated polysesquisiloxane(POSS)to obtain POSS-g-PBO@TA-APTES fbers.The POSS-g-PBO@TA-APTES fbers reinforced bisphenol A dicyanate ester(BADCy)resins(POSS-g-PBO@TAAPTES fbers/BADCy)wave-transparent laminated composites are prepared.The interlaminar shear strength and fexural strength of POSS-g-PBO@TA-APTES fbers/BADCy composites are respectively enhanced from 36.7 and 587.4 MPa to 42.8 and 645.8 MPa,increased by 16.6%and 9.9%compared with those of PBO fbers/BADCy composites.At 1 MHz,the corresponding dielectric constant and dielectric loss are reduced to 2.85 and 0.0047,respectively,lower than those of PBO fbers/BADCy(3.06 and 0.006)composites.Meanwhile,the simulated wave transmittance rate of POSS-g-PBO@TA-APTES fbers/BADCy composites with the thicknesses of 1.5–3.5 mm is higher than 86.2%at 0.3–40 GHz.The volume resistivity and breakdown strength of POSS-g-PBO@TA-APTES fbers/BADCy composites are 2.8×10^(15)Ω·cm and 19.80 kV/mm,higher than PBO fbers/BADCy composites(2.2×10^(15)Ω·cm and 17.69 kV/mm),respectively.And the corresponding heat resistant index is 221.5℃,lower than PBO fbers/BADCy composites(229.6℃).展开更多
基金supported by the Major Program of National Agricultural Science and Technology of China(NK20220607)the West Light Foundation of the Chinese Academy of Sciences(2022XBZG_XBQNXZ_A_001)the Sichuan Science and Technology Program,China(2022ZDZX0014)。
文摘Grain weight is one of the key components of wheat(Triticum aestivum L.)yield.Genetic manipulation of grain weight is an efficient approach for improving yield potential in breeding programs.A recombinant inbred line(RIL)population derived from a cross between W7268 and Chuanyu 12(CY12)was employed to detect quantitative trait loci(QTLs)for thousand-grain weight(TGW),grain length(GL),grain width(GW),and the ratio of grain length to width(GLW)in six environments.Seven major QTLs,QGl.cib-2D,QGw.cib-2D,QGw.cib-3B,QGw.cib-4B.1,QGlw.cib-2D.1,QTgw.cib-2D.1 and QTgw.cib-3B.1,were consistently identified in at least four environments and the best linear unbiased estimation(BLUE)datasets,and they explained 2.61 to 34.85%of the phenotypic variance.Significant interactions were detected between the two major TGW QTLs and three major GW loci.In addition,QTgw.cib-3B.1 and QGw.cib-3B were co-located,and the improved TGW at this locus was contributed by GW.Unlike other loci,QTgw.cib-3B.1/QGw.cib-3B had no effect on grain number per spike(GNS).They were further validated in advanced lines using Kompetitive Allele Specific PCR(KASP)markers,and a comparison analysis indicated that QTgw.cib-3B.1/QGw.cib-3B is likely a novel locus.Six haplotypes were identified in the region of this QTL and their distribution frequencies varied between the landraces and cultivars.According to gene annotation,spatial expression patterns,ortholog analysis and sequence variation,the candidate gene of QTgw.cib-3B.1/QGw.cib-3B was predicted.Collectively,the major QTLs and KASP markers reported here provide valuable information for elucidating the genetic architecture of grain weight and for molecular marker-assisted breeding in grain yield improvement.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24030402)Sichuan Science and Technology Program.
文摘Grain size and weight are key components of wheat yield.Exploitation of major underlying quantitative trait loci(QTL)can improve yield potential in wheat breeding.A recombinant inbred line(RIL)population was constructed to detect QTL for thousand-grain weight(TGW),grain length(GL)and grain width(GW)across eight environments.Genomic regions associated with grain size and grain weight were identified on chromosomes 4A and 6A using bulked segregant exome sequencing(BSE-Seq)analysis.After constructing genetic maps,six major QTL detected in at least four individual environments and in best linear unbiased estimator(BLUE)datasets,explained 7.50%-23.45%of the phenotypic variation.Except for QGl.cib-4A,the other five QTL were co-located in two regions,namely QTgw/Gw.cib-4A and QTgw/Gw/Gl.cib-6A.Interactions of these QTL were analyzed.Unlike QTgw/Gw/Gl.cib-6A,QTgw/Gw.cib-4A and QGl.cib-4A had no effect on grain number per spike(GNS).The QTL were validated in a second cross using Kompetitive Allele Specific PCR(KASP)markers.Since QTgw/Gw.cib-4A was probably a novel locus,it and the KASP markers reported here can be used in wheat breeding.TraesCS4A03G0191200 was predicted to be potential candidate gene for QTgw/Gw.cib-4A based on the sequence differences,spatiotemporal expression patterns,gene annotation and haplotype analysis.Our findings will be useful for fine mapping and for marker-assisted selection in wheat grain yield improvement.
基金supported by the grants from the National Natural Science Foundation of China(30770310,30970435)the Natural Science Foundation of Zhejiang,China(Y307453)
文摘Advertisement calls play an important role in influencing female mate choice and are regarded as one of the key characters responsible for reproductive isolation and speciation events in frogs and toads. The black-spined toad, Bufo melanostictus, is one of the most common amphibians in Southeast Asia and is frequently observed in human habitations throughout its distributional range. The advertisement calls of the toad have not been well described. Here, we report the advertisement calls of this species from Lishui, Zhejiang, China during its breeding season. The advertisement calls of B. melanostictus are composed of single notes with a single harmonic frequency emitted sporadically. The call parameters recorded in this study were highly variable across recording times and with different body sizes. Dominant frequency was negatively correlated with body size. Comparing the calls of the B. melanostictus population from Lishui with the populations from five other localities(Bangkok, Thailand; Coorg, India; Burma, Myanmar; Bali, Indonesia; Halimun-Salak National Park, Indonesia), we found that call structure was similar among five different populations with no harmonics, while the Halimun-Salak National Park population contained two clear harmonics. Dominant frequency was similar between Bali(1.56 kHz) and Coorg(1.6 kHz) populations, while it was highly variable among other populations. Besides call duration, call interval and pulse/call rate may also be significantly different among the comparing populations. Pooling the available data, we observed some similarities and differences in call parameters among the six populations.
基金This work was financially supported by the National Key Research and Development Program of China(2018YFB0703801,2018YFB0703802,2016YFB0701303,and 2016YFB0701304)CRRC Tangshan Co.,Ltd.(201750463031).Special thanks to Professor Hong Wang at Shanghai Jiao Tong University for the fruitful discussions and the constructive suggestions/comments.
文摘A data identifier(DID)is an essential tag or label in all kinds of databases—particularly those related to integrated computational materials engineering(ICME),inheritable integrated intelligent manufacturing(I3M),and the Industrial Internet ofThings.With the guidance and quick acceleration of the developme nt of advanced materials,as envisioned by official documents worldwide,more investigations are required to construct relative numerical standards for material informatics.This work proposes a universal DID format consisting of a set of build chains,which aligns with the classical form of identifier in both international and national standards,such as ISO/IEC 29168-1:2000,GB/T 27766-2011,GA/T 543.2-2011,GM/T 0006-2012,GJB 7365-2011,SL 325-2014,SL 607-201&WS 363.2-2011,and QX/T 39-2005.Each build chain is made up of capital letters and numbers,with no symbols.Moreover,the total length of each build chain is not restricted,which follows the formation of the Universal Coded Character Set in the international standard of ISO/IEC 10646.Based on these rules,the proposed DID is flexible and convenient for extendi ng and sharing in and between various cloud-based platforms.Accordingly,classical two-dimensional(2D)codes,including the Hanxin Code,Lots Perception Matrix(LP)Code,Quick Response(Q.R)code,Grid Matrix(GM)code,and Data Matrix(DM)Code,can be constructed and precisely recognized and/or decoded by either smart phones or specific machines.By utilizing these 2D codes as the fingerprints of a set of data linked with cloud-based platforms,progress and updates in the composition-processing-structure-property-performance workflow process can be tracked spontaneously,paving a path to accelerate the discovery and manufacture of advanced materials and enhance research productivity,performance,and collaboration.
基金funded by grants from the National Natural Science Foundation of China(31270443,31500329)the Science Foundation of Zhejiang Provincial Committee of Education(Y201534237)the Scientific Research Foundation of Ph.D.,Lishui University(QD1423)
文摘Alien species are one of the most serious threats to the decline and extinction of native amphibian populations. In this study, we examined the predation of invasive Western Mosquitofish Gambusia affinis on the eggs, embryos, and tadpoles of Duttaphrynus melanostictus and Pelophylax nigromaculatus in south China. Our results suggested that the survival of eggs and embryos remaining in the egg capsules of P. nigromaculatus and D. melanostictus was significantly higher than those removed from the egg capsule at 12-h intervals within 72 h in the presence of G. affinis. The survival of P. nigromaculatus eggs and embryos without egg capsules was significantly lower than those of D. melanostictus without egg capsules. The survival of P. nigromaculatus eggs and embryos with egg capsules was significantly higher than those of D. melanostictus with egg capsules from 24 h to 72 h except for 12 h. The survival of D. melanostictus tadpoles was significantly higher than that of P. nigromaculatus tadpoles in the presence of G. affinis. The survival of Gosner stage 26 tadpoles of P. nigromaculatus was significantly higher than that of Gosner stage 30 tadpoles from 12 h to 60 h, but there were no significant differences at 72 h. In contrast, the survival of Gosner stage 26 tadpoles of D. melanostictus was significantly lower than that of Gosner stage 30 tadpoles within 72 h, recording every 12 h. The increasing temperature caused a significant increase in predation by G. affinis on P. nigromaculatus eggs and embryos. The outer jelly capsule surrounding anurans eggs might serve as a mechanical defense against predation by G. affinis due to its large diameter, relatively stationary state and unpalatability. The differences in the vulnerability of P. nigromaculatus and D. melanostictus embryos and tadpoles to G. affinis probably due to differences in the unpalatability, black skin and activity. Based on the magnitude of predation by G. affinis on the eggs, embryos and tadpoles of these two species and the combined impact of temperature, we might speculate that invasive G. affinis and global warming would have more detrimental impacts on population viability of P. nigromaculatus than D. melanostictus in China.
基金supported by the grants from the National Natural Science Foundation of China (30970435,31270443)the Natural Science Foundation of Zhejiang, China (LY13C030004)
文摘Kinship and density are believed to affect important ecological processes such as intraspecific competition, predation, growth, development, cannibalism, habitat selection and mate choice, In this work, we used Chinese tiger frog Hoplobatrachus chinensis tadpoles as an experimental model to investigate the effects of kinship and density on growth and development of this species over a 73 day period. The results showed that density can affect the growth and developmental traits (survival rate, larval period, size at the limb bud protrusion/metamorphic climax and body mass at different life stages) of H. chinensis tadpoles, while kinship does not. Tadpoles took longer to develop and potential metamorphosis was greater in high density groups of both sibling and non-siblings. The interaction of kinship and density did not significantly influenced growth traits of H. chinensis tadpoles during the experimental period. For coefficient variations of each growth trait, no differences were detected between sibling and non-sibling groups. These findings provide valuable information on the basic ecology of H. chinensis which will be helpful in future studies of other anuran species.
基金supported by the Project sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry (No.Z2005-2-45006)
文摘Lung cancer is one of the most important causes of cancer-related mortality worldwide. Human cytochrome P450 2A13 enzyme (CYP2A13) is predominantly expressed in the respiratory tract and could catalyze various carcinogens. In this study, we quantified CYP2A13 expression in non-small cell lung cancer (NSCLC) tissues and examined the relation between CYP2A13 and clinicopathologic factors. Thirty-five paired lung cancer and normal tissues were studied for the expression of the CYP2A13 gene by using real-time PCR and Western blot- ting assays. We also investigated the relationship between CYP2A13 expression and clinicopathologic factors such as age, gender, histology and lymph node status in tumor tissues. SPSS (17.0) statistical software was applied for data analysis. The real-time PCR results showed that there was no significant difference in the CYP2A13 mRNA transcript levels between tumor and paired normal tissues in the 35 samples and in 12 paired squamous cell car- cinomas. In adenocarcinoma, the expression of CYP2A13 mRNA in tumor tissues was 12.5% of that in adjacent tissues (P 〈 0.05) and it was not associated with age, gender, histology and lymph node status of the patients. The amounts of CYP2A13 proteins detected by Western blotting assays correlated well with those of the correspond- ing mRNAs. In conclusion, the expression of CYP2A13 was downregulated in lung adenocarcinoma. CYP2A13 may be involved in the development and progression of lung adenocarcinoma.
基金the National Natural Science Foundation of China, No. 30300106
文摘BACKGROUND: Expression of Fos in neurons of periaqueductal gray (PAG) is used to reflect the excitability. However, changes of expression of Fos in neurons of PAG are caused by injured electrostimulation after simulated weightlessness, and the relationship between pretreatment and injection of succinylcholine has not been determined yet. OBJECTIVE : To investigate the changes of expression of Fos in PAG induced by injured electrostimulation pretreatment and injection of succinylcholine at 2 weeks after simulated weightlessness.DESIGN: Observational and controlled animal study.SETTING: Department of Physiology, Medical School, Xi'an Jiaotong University; Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education. MATERIALS: A total of 24 adult female SD rats, of clean grade and weighing 180-220 g, were selected in this study. METHODS: The experiment was completed in the Experimental Animal Center of Xi'an Jiaotong University.① All rats were randomly divided into 2 groups according to body mass: simulated weightlessness group and control group with 12 in each group. And then, each group was also divided into 3 subgroups: electrostimulation group, succinylcholine-pretreatment group and succinylcholine-injection group with 4 in each subgroup. ②The model of weightlessness was simulated by tail-suspended female rats, which were described and modified by Cheng Jie. Rats in normal control group were given the same interventions as simulated weightlessness group except for tail-suspended. ③ Experimental method: The rats in electrostimulation group were given nociceptive stimulus by a pair of subcutaneous electrodes inserted into 1 and 5 claw of left hindlimb. The stimulus (current: 10 mA; duration: 1 ms; interval: 1 s) lasted for 30 minutes. The rats in succinylcholine-pretreatment group received stimulus after intravenous administration of succinylcholine, rats in succinylcholine-injection group were not given stimulus, just received succinylcholine. ④ All rats were perfused and fixed after 2 hours from the end of stimulation. The brains were removed, and serial frozen sections of midbrain were stained using immunocytochemical method, observed and taken photos under light-microscope. The number and morphological characters of Fos-immunoreactive (Fos-IR) neurons in ventrolateral part of PAG were investigated. MAIN OUTCOME MEASURES: The alterations in number and morphological characters of Fos-IR neurons in ventrolateral PAG of all rats.RESULTS: A total of 24 rats were involved in the final analysis. ① The morphological changes of Fos-IR neurons: The expressions of Fos in ventrolateral part of PAG were observed in both control and simulated weightlessness groups rats after being given nociceptive stimulus. As compared with control group, Fos-IR neurons in simulated weightlessness group were dyed lightly, cellular integrity was impaired, and cellular verge was unclear. ② The numbers of Fos-IR neurons: In control group, the numbers of Fos-IR neurons in ventrolateral part of PAG in simulated weightlessness group were obviously lower than succinylcholine-pretreatment group, but obviously higher than succinylcholine-injection group (46.94±3.38, 71.06±8.96 and 35.04±4.62, respectively, P 〈 0.05). In 14-day simulated weightlessness group, the numbers of Fos-IR neurons in electrostimulation group were also obviously lower than succinylcholine-pretreatment group, and obviously higher than succinylcholine-injection group (27.77±3.27, 32.91±2.99 and 11.75±1.00, respectively, P 〈 0.05). The numbers of Fos-IR neurons in all subgroups in control group were obviously higher than those subgroups in simulated weightlessness group. Compared with electrostimulation group, the percentage of expression of Fos in ventrolateral part of PAG responsed to nociceptive stimulus after administration of succinylcholine (SCH) was increased to 51.83% in control group and 18.51% in simulated weightlessness group.CONCLUSION :① The expression of Fos in neurons in ventrolateral part of PAG were increased by the pretreatment of SCH before nociceptive stimulus.② Nociceptive stimulus could increase the expression of Fos in neurons in ventrolateral part of PAG. ③ The numbers of Fos-IR neurons in ventrolateral part of PAG were decreased obviously after 2-week simulated weightlessness.
基金the National Natural Science Foundation of China, No. 30270445
文摘BACKGROUND: Prenatal stress has been shown to inhibit cell proliferation in the dentate gyrus and hippocampus, reduce hippocampal volume, and cause neuronal loss and oxidative damage in the hippocampus of offspring rats, but the sexual difference of the effects on offsprings is seldom referred to. OBJECTIVE: To observe the effect of prenatal stress to adult pregnant rats on expression of extracellular signal-regulated kinases (ERK) in hippocampus of the offspring rats of different genders. DESIGN : A randomized and control animal experiment.SETTING: Department of Physiology and Pathophysiology, School of Medicine, Xi'an Jiaotong University. MATERIALS : The experiments were carried out in the Key Laboratory of Environment and Gene Related Diseases (Xi'an Jiaotong University), Ministry of Education between October 2005 and March 2006. Fifteen female and five male adult Sprague-Dawley rats were adopted. Female rats weighing 230-250 g and male rats weighing 280-350 g were used. METHODS: The virgin female rats were placed overnight with adult male rats (3:1) for mating. A total of twelve pregnant rats were randomly assigned to prenatal stress group (PNS group, n=6) and control group (n=6). The pregnant rats of the PNS group were exposed to restraint stress on days 14-20 of pregnancy three times a day, 45 minutes for each time . The restraint device was a transparent plastic tube (6.8 cm in diameter) with air holes for breathing and closed end. The length could be adjusted to accommodate the size of the animals. To prevent habituation of animals to the daily procedure, restraint periods were randomly shifted within certain time periods (8:00-11:00, 11:00-14:00, and 16:00-19:00). After birth, offsprings of all groups were culled to 8-10 litters in each group and housed in the same animal room, and kept together with their biologic mothers. The pregnant rats of the control group were left undisturbed. On day 21, after all the offspring were weaned, male and female pups were separated and housed four in each cage respectively until test at 30 days of age. At the end of postnatal day 30, one male and female offspring rats from the same dam were selected with a random choice and a total of 24 animals from 12 different dams were used. The experimental rats were sacrificed by decapitation under anesthesia. Bilateral hippocampal tissues were isolated and homogenized in cold condition. Alkaline carbonate buffer (BCA) method was used to detect the concentration of extracellular signal-regulated kinases (ERK), then mixed with loading buffer, the constant voltage was 100 V. Finally, BCIP/NBT staining and electrDphoresis were performed, the absorbance (A) value for the bands was detected with the Bandscan analytical software, and the expression of ERK in hippocampus of offspring rats of different genders in each group was quantitatively analyzed. MAIN OUTCOME MEASURES: The level of ERK expression in hippocampus of offspring rats of different genders in each group was observed.RESULTS: All the 24 offspring rats were involved in the analysis of results. ① The staining results of ERP activity in the extract of brain tissue detected with Western blotting technique and specific antibody analysis showed that the ERP in hippocampus of offspring rats had two subtypes of ERK-1 and ERK-2, and the latter was the main type.② Standardized by the average A value in the control group, the quantitative data of the general A value of total ERK showed that the expression of ERK-2 in hippocampus of female offspring rats was obviously higher in the PNS group than in the control group (A value: 126±6.76,100±4.89,P〈 0.01). ③The expression of ERK-2 had no obvious difference between the female and male offspring rats in the control group.④ The expression of ERK-2 in hippocampus of male offspring rats was a little higher in the PNS group than in the control group (A value: 104±6.27,102±5.48,P 〉 0.05). CONCLUSION : PNS significantly affects the increase of ERK expression in hippocampus of female offspring rats, but has no obvious influence on that of male ones.
基金The authors are grateful for the support and funding from National Scientific Research Project(Basis Strengthening Plan)State Key Laboratory of Solidification Processing in NWPU(No.SKLSP202103).
文摘Bisphenol A dicyanate ester resins modified by fluorine-containing liquid crystal compound(LCFE)are applied as polymer matrix(LCFE-BADCy),poly(p-phenylene-2,6-benzobisoxazole)(PBO)fibers as rein-forcements,and fluorine/adamantane PBO precursor(pre FABPBO)as interfacial compatibilizer to prepare the corresponding PBO fibers/FABPBO/LCFE-BADCy wave-transparent laminated composites.LCFE could improve the order degree of BADCy cured network,in favor of enhancing the wave-transparent perfor-mance,mechanical properties,and intrinsic thermal conductivity.The dielectric constant and dielectric loss of PBO fibers/FABPBO/LCFE-BADCy composites are highly temperature(25–200℃)and frequency(10^(4)–10^(7) Hz and 8.2–12.4 GHz)stable with the value of 2.49 and 0.003 under 10^(6) Hz at 25℃,and the corresponding wave transmission efficiency is 95.0%,higher than that of 92.5%for PBO fibers/BADCy com-posites.The interlamellar shear strength and flexural strength are respectively 50.7 MPa and 682.5 MPa,38.1%and 16.2%higher than those of PBO fibers/BADCy composites.Besides,the volume resistivity,breakdown voltage,heat resistance index,glass transition temperature,flame retardant grade,and ul-timate oxygen index of PBO fibers/FABPBO/LCFE-BADCy composites are respectively 5.3×10^(15)Ωcm,29.75 kV/mm,217.2℃,245.7℃,V-1 grade,and 33.6%,expected to be performed as a new generation of“lightweight/loading/wave-transparent”electromagnetic window materials in advanced military weapons and civil communication base station.
基金the support and funding from the National Scientific Research Project(Basis Strengthening Plan)and State Key Laboratory of Solidification Processing in NWPU(SKLSP202103).
文摘Comprehensive Summary Branched fluorine/adamantane PBO precursor(preFABPBO),synthesized via random co-condensation between 2,2-bis(3-amino-4-hydroxyphenyl)hexafluoro propane,1,3-adamantanedicarbonyl dichloride,and trimesoyl chloride,is performed as interfacial compatibilizer,bisphenol A dicyanate ester(BADCy)resin as polymer matrix,and poly(p-phenylene-2,6-benzobisoxazole)(PBO)fibers as reinforcements to prepare PBO fibers/FABPBO-BADCy wave-transparent laminated composites by high temperature molding.The mechanical properties,wave-transparent performances,and heat resistances of PBO fibers/BADCy composites are simultaneously improved by the addition of preFABPBO.The interlaminar shear strength(ILSS)and flexural strength of PBO fibers/FABPBO-BADCy composites are 48.9 and 665.3 MPa,respectively,increased by 33.2%and 13.3%compared to those of PBO fibers/BADCy composites(36.7 and 587.4 MPa).The dielectric constant and dielectric loss values at 106 Hz are 2.53 and 0.003,respectively,lower than those of PBO fibers/BADCy composites(3.06 and 0.006),and the corresponding wave transmission efficiency is 94.8%,which also presents excellent stability over the wide temperature(25-200℃)and frequency range(103-107 Hz and 8.2-12.4 GHz).Meanwhile,the heat resistance index and glass transition temperature are 229.9℃and 247.5℃,also better than those of PBO fibers/BADCy composites(229.6℃and 247.1℃).
基金supported by grants from the National Natural Science Foundation of China(No.82100552)the Science and Technology Department of Sichuan Province of China(No.2021YJ0468)the PostDoctor Research Project,West China Hospital,Sichuan University(No.2020HXBH085)
文摘To the Editor:Autoimmune liver disease is a chronic immune-mediated liver disease.The term“overlap syndrome”(OS)is used to describe the conditions in which patients simultaneously or consecutively present with features of primary biliary cholangitis(PBC)or primary sclerosing cholangitis and autoimmune hepatitis(AIH).[1]PBC-AIH OS is described most frequently,[2]with a prevalence of approximately 8%to 20%in adult patients with either pure PBC or AIH.
基金The authors thank for the financial support from the National Defense Technology Innovation Special Zone Spark Project(No.2016300TS00911901)the Natural Science Foundation of Jiangsu Province(No.BK20210616),and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Metal-organic frameworks(MOFs)and covalent organic frameworks(COFs)as the novel porous materials have the merits of diverse,adjustable functionality,high porosity and surface area,which have great application prospects in the gas storage,separation and catalysis.In addition,their derivates make up for the insufficient of electronic conductivity and chemical stability of MOFs and COFs,and provide a new ideal for accurate control of material structure.Up to now,many efficient electrocatalysts have been designed based on MOFs,COFs and their derivates for O_(2)reduction/evolution reactions(ORR/OER)and CO_(2)reduction/evolution reactions(CO_(2)RR/CO_(2)ER)in the metal-air batteries.In this review,the latest development of MOFs,COFs and their derivates in the metal-air batteries is summarized,and we discuss the structural characteristics of these materials and their corresponding mechanisms of action.By comprehensively reviewing the advantages,challenges and prospects of MOFs and COFs,we hope that the organic framework materials will shed more profound insights into the development of electrocatalysis and energy storage in the future.
基金supported by the National Natural Science Foundation of China(grant nos.31921005 and 31991211)the Strategic Priority Research Program of the Chinese Academy of Sciences(grant nos.XDA24010104,XDA24010204,and XDA24030102)+1 种基金the Special Information Program of the Chinese Academy of Sciences(grant no.XXH13506-408)the China Agricultural Research System(grant no.CARS-03).
文摘Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait.
基金This work was supported by the National Defense Technology Innovation Special Zone Spark Project[2016300TS00911901].
文摘Metal-organic framework materials(MOFs)have been widely stu-died because of their adjustable composition and controllable structure in the field of microwave absorption(MA).Therein,Prussian blue analogs(PBA)have attracted the attention of researchers with ultra-high metal content.However,the attenua-tion ability of microwave for PBA-based composites is still unsatis-factory up to now.Therefore,the NiFe/CoFe@C composites were prepared by carbonizing polymetallic PBA(NiCoFe PBA)materials in this work,and the influence of different metal alloy components on MA was explored by adjusting the ratio of metal ions(Ni^(2+)/Co^(2+)).Moreover,the NiFe/CoFe@C composites have rich interfaces and enhance the polarization loss due to the introduction of Ni and it has an optimal performance at 2.7 mm that is the reflection loss(RL)is−41.49 dB and an effective absorption bandwidth(EAB)is 7.12 GHz with 1/1(Ni^(2+)/Co^(2+)).The above data provides a research idea for obtaining light and efficient absorbers.
基金The authors are grateful for the support and funding from National Scientifc Research Project(Basis Strengthening Plan)China Postdoctoral Science Foundation(2019M653735)+3 种基金State Key Laboratory for Modifcation of Chemical Fibers and Polymer Materials from Donghua University(KF2001)State Key Laboratory of Solidifcation Processing in NWPU(SKLSP202103)L.Tang thanks for the Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University(CX2021036)This work is also fnancially supported by Polymer Electromagnetic Functional Materials Innovation Team of Shaanxi Sanqin Scholars.
文摘Hybrid polymer membrane(TA-APTES),synthesized by tannic acid(TA)and aminopropyl trethoxysilane(APTES)based on the Schif’s base and Michael addition reaction,is deposited on the surface of poly(p-phenylene-2,6-benzobisoxazole)(PBO)fbers,and then grafted with epoxy-terminated polysesquisiloxane(POSS)to obtain POSS-g-PBO@TA-APTES fbers.The POSS-g-PBO@TA-APTES fbers reinforced bisphenol A dicyanate ester(BADCy)resins(POSS-g-PBO@TAAPTES fbers/BADCy)wave-transparent laminated composites are prepared.The interlaminar shear strength and fexural strength of POSS-g-PBO@TA-APTES fbers/BADCy composites are respectively enhanced from 36.7 and 587.4 MPa to 42.8 and 645.8 MPa,increased by 16.6%and 9.9%compared with those of PBO fbers/BADCy composites.At 1 MHz,the corresponding dielectric constant and dielectric loss are reduced to 2.85 and 0.0047,respectively,lower than those of PBO fbers/BADCy(3.06 and 0.006)composites.Meanwhile,the simulated wave transmittance rate of POSS-g-PBO@TA-APTES fbers/BADCy composites with the thicknesses of 1.5–3.5 mm is higher than 86.2%at 0.3–40 GHz.The volume resistivity and breakdown strength of POSS-g-PBO@TA-APTES fbers/BADCy composites are 2.8×10^(15)Ω·cm and 19.80 kV/mm,higher than PBO fbers/BADCy composites(2.2×10^(15)Ω·cm and 17.69 kV/mm),respectively.And the corresponding heat resistant index is 221.5℃,lower than PBO fbers/BADCy composites(229.6℃).