Expert consensus on irrigation and intracanal medication in root canal therapy

Chemical cleaning and disinfection are crucial steps for eliminating infection in root canal treatment. However, irrigant selection or irrigation procedures are far from clear. The vapor lock effect in the apical region has yet to be solved, impeding irrigation efficacy and resulting in residual infections and compromised treatment outcomes.

Expert consensus on difficulty assessment of endodontic therapy

Endodontic diseases are a kind of chronic infectious oral disease. Common endodontic treatment concepts are based on the removal of inflamed or necrotic pulp tissue and the replacement by gutta-percha. However, it is very essential for endodontic treatment to debride the root canal system and prevent the root canal system from bacterial reinfection after root canal therapy(RCT). Recent research, encompassing bacterial etiology and advanced imaging techniques, contributes to our understanding of the root canal system’s anatomy intricacies and the technique sensitivity of RCT. Success in RCT hinges on factors like patients, infection severity, root canal anatomy, and treatment techniques. Therefore, improving disease management is a key issue to combat endodontic diseases and cure periapical lesions. The clinical difficulty assessment system of RCT is established based on patient conditions, tooth conditions, root canal configuration, and root canal needing retreatment, and emphasizes pre-treatment risk assessment for optimal outcomes. The findings suggest that the presence of risk factors may correlate with the challenge of achieving the high standard required for RCT. These insights contribute not only to improve education but also aid practitioners in treatment planning and referral decision-making within the field of endodontics.

Experts consensus on the procedure of dental operative microscope in endodontics and operative dentistry

The dental operative microscope has been widely employed in the field of dentistry,particularly in endodontics and operative dentistry,resulting in significant advancements in the effectiveness of root canal therapy,endodontic surgery,and dental restoration.However,the improper use of this microscope continues to be common in clinical settings,primarily due to operators’insufficient understanding and proficiency in both the features and established operating procedures of this equipment.In October 2019,Professor Jingping Liang,Vice Chairman of the Society of Cariology and Endodontology,Chinese Stomatological Association,organized a consensus meeting with Chinese experts in endodontics and operative dentistry.The objective of this meeting was to establish a standard operation procedure for the dental operative microscope.Subsequently,a consensus was reached and officially issued.Over the span of about four years,the content of this consensus has been further developed and improved through practical experience.

Numerical Simulation of Motor Vehicles’ Courtesy Behavior on Crosswalk Based on Car-Following Model

In observing driver courtesy towards pedestrians at unsignalized crosswalks, a behavioral model was adopted in a simulation based on the GM Car-Following Model. The SIMI Motion Software was used to extract the vehicle operation data from Wenyi South Road and Hanyuan Road in Xi'an City. The parameters of the GM Car-Following Model were calibrated by genetic algorithm. The road simulation environment based on the Car-Following Model was constructed by MATLAB. In the case of no stopping, uniform deceleration avoidance with advance notice, emergency brake avoidance without advance notice, changes such as the displacement of the Car-Following queue, headway, speed, acceleration, and deceleration were analyzed by numerical simulation. The results show that when there is advance notice before the crosswalk, the minimum headway distances of Car1-Car2, Car2-Car3, Car3-Car4 and Car4-Car5 are 7.09 m, 7.38 m, 7.65 m, 7.91 m, and the average rates of change of the headway during deceleration are 0.78 m/s, 0.74 m/s, 0.71 m/s, 0.68 m/s respectively;in the absence of advance notice before the crosswalk, the minimum headway distances of Car1-Car2, Car2-Car3, Car3-Car4 and Car4-Car5 are 7.28 m, 7.75 m, 8.19 m, 8.59 m, and the average rates of change of the headway during deceleration are 1.57 m/s, 1.25 m/s, 1.04 m/s, 0.96 m/s, respectively. Therefore, in order to effectively prevent the occurrence of vehicle rear-end events, it's necessary to set traffic signs and markings on the preceding section of the intersection or road exhibiting behavioral comity.

Gut microbiota-derived short-chain fatty acids ameliorate methamphetamine-induced depression-and anxiety-like behaviors in a Sigmar-1 receptor-dependent manner

Methamphetamine(Meth)abuse can cause serious mental disorders,including anxiety and depression.The gut microbiota is a crucial contributor to maintaining host mental health.Here,we aim to investigate if microbiota participate in Meth-induced mental disorders,and the potential mechanisms involved.Here,15 mg/kg Meth resulted in anxiety-and depression-like behaviors of mice successfully and suppressed the Sigma-1 receptor(SIGMAR1)/BDNF/TRKB pathway in the hippocampus.Mean-while,Meth impaired gut homeostasis by arousing the Toll-like receptor 4(TLR4)-related colonic inflammation,disturbing the gut microbiome and reducing the microbiota-derived short-chain fatty acids(SCFAs).Moreover,fecal microbiota from Meth-administrated mice mediated the colonic inflam-mation and reproduced anxiety-and depression-like behaviors in recipients.Further,SCFAs supple-mentation optimized Meth-induced microbial dysbiosis,ameliorated colonic inflammation,and repressed anxiety-and depression-like behaviors.Finally,Sigmarl knockout(Sigmar1^(-/-))repressed the BDNF/TRKB pathway and produced similar behavioral phenotypes with Meth exposure,and elim-inated the anti-anxiety and-depression effects of SCFAs.The activation of SIGMAR1 with fluvoxamine attenuated Meth-induced anxiety-and depression-like behaviors.Our findings indicated that gut microbiota-derived SCFAs could optimize gut homeostasis,and ameliorate Meth-induced mental disorders in a SIGMAR1-dependent manner.This study confirms the crucial role of microbiota in Methrelated mental disorders and provides a potential preemptive therapy.

Mechanical performance analysis and stiffness test of a new type of suspension bridge

A new type of suspension bridge is proposed based on the gravity stiffness principle.Compared with a conventional suspension bridge,the proposed bridge adds rigid webs and cross braces.The rigid webs connect the main cable and main girder to form a truss that can improve the bending stiffness of the bridge.The cross braces connect the main cables to form a closed space truss structure that can improve the torsional stiffness of the bridge.The rigid webs and cross braces are installed after the construction of a conventional suspension bridge is completed to resist different loads with different structural forms.A new type of railway suspension bridge with a span of 340 m and a highway suspension bridge with a span of 1020 m were designed and analysed using the finite element method.The stress,deflection of the girders,unbalanced forces of the main towers,and natural frequencies were compared with those of conventional suspension bridges.A stiffness test was carried out on the new type of suspension bridge with a small span,and the results were compared with those for a conventional bridge.The results showed that the new suspension bridge had a better performance than the conventional suspension bridge.

MiR-711 and miR-183-3p as potential markers for vital reaction of burned skin

In forensic practice,the identification of antemortem burns and postmortem burns is of the utmost importance.Reports from previous studies have shown that miRNAs,with lengths stretching over 18–25 nucleotides,are highly stable and resistant to degradation.However,there has been little research into the application of miRNAs in identifying antemortem and postmortem burns.This study compared the expression of miR-711 and miR-183-3p levels in mouse and postmortem human burned skins using RT-qPCR assay.RT-qPCR examination of burned mouse skins showed that increased miR-711 and miR-183-3p expression in comparison to intact skin tissues.The increased expressions of these two miRNAs were observed until 120 h after death in burned mouse skins,whereas no significant changes were found in postmortem burned skins.In human burned skins,the increased levels of these two miRNAs at 48 h following autopsy occurred in 19 of 26 subjects,which appeared to be related to the severity of the burn.These findings suggest that miR-711 and miR-183-3p may act as biomarkers for vital reaction of skin burn.