Influence of layer thickness on formation quality,microstructure,mechanical properties,and corrosion resistance of WE43 magnesium alloy fabricated by laser powder bed fusion

Laser powder bed fusion(L-PBF)of Mg alloys has provided tremendous opportunities for customized production of aeronautical and medical parts.Layer thickness(LT)is of great significance to the L-PBF process but has not been studied for Mg alloys.In this study,WE43 Mg alloy bulk cubes,porous scaffolds,and thin walls with layer thicknesses of 10,20,30,and 40μm were fabricated.The required laser energy input increased with increasing layer thickness and was different for the bulk cubes and porous scaffolds.Porosity tended to occur at the connection joints in porous scaffolds for LT40 and could be eliminated by reducing the laser energy input.For thin wall parts,a large overhang angle or a small wall thickness resulted in porosity when a large layer thicknesses was used,and the porosity disappeared by reducing the layer thickness or laser energy input.A deeper keyhole penetration was found in all occasions with porosity,explaining the influence of layer thickness,geometrical structure,and laser energy input on the porosity.All the samples achieved a high fusion quality with a relative density of over 99.5%using the optimized laser energy input.The increased layer thickness resulted to more precipitation phases,finer grain sizes and decreased grain texture.With the similar high fusion quality,the tensile strength and elongation of bulk samples were significantly improved from 257 MPa and 1.41%with the 10μm layer to 287 MPa and 15.12%with the 40μm layer,in accordance with the microstructural change.The effect of layer thickness on the compressive properties of porous scaffolds was limited.However,the corrosion rate of bulk samples accelerated with increasing the layer thickness,mainly attributed to the increased number of precipitation phases.

Improving corrosion resistance of additively manufactured WE43 magnesium alloy by high temperature oxidation for biodegradable applications

Laser powder bed fusion(L-PBF)has been employed to additively manufacture WE43 magnesium(Mg)alloy biodegradable implants,but WE43 L-PBF samples exhibit excessively rapid corrosion.In this work,dense WE43 L-PBF samples were built with the relativity density reaching 99.9%.High temperature oxidation was performed on the L-PBF samples in circulating air via various heating temperatures and holding durations.The oxidation and diffusion at the elevated temperature generated a gradient structure composed of an oxide layer at the surface,a transition layer in the middle and the matrix.The oxide layer consisted of rare earth(RE)oxides,and became dense and thick with increasing the holding duration.The matrix was composed ofα-Mg,RE oxides and Mg_(24)RE_(5) precipitates.The precipitates almost disappeared in the transition layer.Enhanced passivation effect was observed in the samples treated by a suitable high temperature oxidation.The original L-PBF samples lost 40%weight after 3-day immersion in Hank’s solution,and broke into fragments after 7-day immersion.The casted and solution treated samples lost roughly half of the weight after 28-day immersion.The high temperature oxidation samples,which were heated at 525℃ for 8 h,kept the structural integrity,and lost only 6.88%weight after 28-day immersion.The substantially improved corrosion resistance was contributed to the gradient structure at the surface.On one hand,the outmost dense layer of RE oxides isolated the corrosive medium;on the other hand,the transition layer considerably inhibited the corrosion owing to the lack of precipitates.Overall,high temperature oxidation provides an efficient,economic and safe approach to inhibit the corrosion of WE43 L-PBF samples,and has promising prospects for future clinical applications.

Application Principle of Digital Animation Technology in Experimental Teaching

With the continuous development and improvement of social science and technology,the“learning-oriented”teaching method is proposed,the traditional experimental teaching methods in colleges and universities are insufficient to meet the needs of experimental teaching.By studying the role and advantages of digital animation technology in experimental teaching,analyzing the application principle of digital animation in laboratory teaching,it is proposed to strengthen the application of digital animation technology in experimental teaching,give full play to the advantages of digital animation technology,and improve the quality of experimental teaching to promote students’learning efficiency in experimental teaching.

Research on the Principle of Optimizing Teaching with Digital Animation in Experimental Teaching

With the rapid development of computing technology,experimental teaching explores new teaching methods using virtual laboratory and digital laboratory.Based on the characteristics of visualization,continuity and simulation,digital animation technology has become an important form of application in experimental teaching reform.This paper analyzes the artistic characteristics of digital animation,and summarizes that digital animation can display the experimental information intuitively,enable students to quickly obtain the cognition of the experimental content,and obtain psychological pleasure through digital animation in experimental learning,thus improving the teaching effect of experimental teaching.

Cross-sectional network analysis of plasma proteins/metabolites correlated with pathogenesis and therapeutic response in acute promyelocytic leukemia

The treatment of PML/RARA+acute promyelocytic leukemia(APL)with all-trans-retinoic acid and arsenic trioxide(ATRA/ATO)has been recognized as a model for translational medicine research.Though an altered microenvironment is a general cancer hallmark,how APL blasts shape their plasma composition is poorly understood.Here,we reported a cross-sectional correlation network to interpret multilayered datasets on clinical parameters,proteomes,and metabolomes of paired plasma samples from patients with APL before or after ATRA/ATO induction therapy.Our study revealed the two prominent features of the APL plasma,suggesting a possible involvement of APL blasts in modulating plasma composition.One was characterized by altered secretory protein and metabolite profiles correlating with heightened proliferation and energy consumption in APL blasts,and the other featured APL plasma-enriched proteins or enzymes catalyzing plasma-altered metabolites that were potential trans-regulatory targets of PML/RARA.Furthermore,results indicated heightened interferon-gamma signaling characterizing a tumor-suppressing function of the immune system at the first hematological complete remission stage,which likely resulted from therapy-induced cell death or senescence and ensuing supraphysiological levels of intracellular proteins.Overall,our work sheds new light on the pathophysiology and treatment of APL and provides an information-rich reference data cohort for the exploratory and translational study of leukemia microenvironment.

中等时间尺度下弹道明胶的线性黏弹性模型

在不同时间尺度下,明胶表现出不同的黏弹性行为。应用蠕变和应力松弛模式研究质量分数为10%的弹道明胶在中等时间尺度下的线性黏弹性行为。低剪切速率下的连续加载-卸载实验显示,在常规感知的时间尺度下(～1min)弹道明胶接近完全弹性体,其线性区应变范围为0～0.25。对蠕变实验对应的中等时间尺度(>1h),选择样品老化24h作为实验起点,这时明胶结构缓慢老化带来的影响可以忽略,从而能够构建恒定参数的本构方程来描述弹道明胶的线性黏弹性行为。对1组蠕变实验结果进行分析,可确定Burgers模型的4个粘弹性参数,然后使用该组参数的模型预测另外2组蠕变实验以及应力松弛实验。结果表明Burgers模型能较好地表征弹道明胶在中等时间尺度下的线性黏弹性行为。

The effect of pore size on the mechanical properties, biodegradation and osteogenic effects of additively manufactured magnesium scaffolds after high temperature oxidation: An in vitro and in vivo study

The effects of pore size in additively manufactured biodegradable porous magnesium on the mechanical properties and biodegradation of the scaffolds as well as new bone formation have rarely been reported. In this work, we found that high temperature oxidation improves the corrosion resistance of magnesium scaffold. And the effects of pore size on the mechanical characteristics and biodegradation of scaffolds, as well as new bone formation, were investigated using magnesium scaffolds with three different pore sizes, namely, 500, 800, and 1400 μm (P500, P800, and P1400). We discovered that the mechanical characteristics of the P500 group were much better than those of the other two groups. In vitro and in vivo investigations showed that WE43 magnesium alloy scaffolds supported the survival of mesenchymal stem cells and did not cause any local toxicity. Due to their larger specific surface area, the scaffolds in the P500 group released more magnesium ions within reasonable range and improved the osteogenic differentiation of bone mesenchymal stem cells compared with the other two scaffolds. In a rabbit femoral condyle defect model, the P500 group demonstrated unique performance in promoting new bone formation, indicating its great potential for use in bone defect regeneration therapy.

中新世气候适宜期构造运动加速全球海洋碳循环

Global warming during the Miocene Climate Optimum(MCO,~17-14 million years ago)is associated with massive carbon emissions sourced from the flood basalt volcanism and ocean crustal production.However,the perturbation of tectonic carbon degassing on the interaction between climate change and carbon cycle remains unclear.Here,through time-evolutive phase analysis of new and published high-resolution benthic foraminiferal oxygen(δ^(18)O)and carbon(δ^(13)C)isotope records from the global ocean,we find that variations in the marine carbon cycle lead the climate-cryosphere system(δ^(13)Clead-δ^(18)O)on 405,000-year eccentricity timescales during the MCO.This is in contrast to the previously reported climate-lead-carbon(δ^(18)O-lead-δ^(13)C)scenario during most of the Oligo-Miocene(~34-6 million years ago).Further sensitivity analysis and model simulations suggest that the elevated atmospheric CO_(2) concentrations and the resulting greenhouse effect strengthened the low-latitude hydrological cycle during the MCO,accelerating the response of marine carbon cycle to eccentricity forcing.Tropical climate processes played a more important role in regulating carbon-cycle variations when Earth's climate was in a warm regime,as opposed to the dominant influence of polar ice-sheet dynamics during the PlioPleistocene(after~6 million years ago).

Biodegradable magnesium alloy WE43 porous scaffolds fabricated by laser powder bed fusion for orthopedic applications:Process optimization,in vitro and in vivo investigation

Laser powder bed fusion(L-PBF)of magnesium(Mg)alloy porous scaffolds is expected to solve the dual challenges from customized structures and biodegradable functions required for repairing bone defects.However,one of the key technical difficulties lies in the poor L-PBF process performance of Mg,contributed by the high susceptibility to oxidation,vaporization,thermal expansion,and powder attachment etc.This work investigated the influence of L-PBF energy input and scanning strategy on the formation quality of porous scaffolds by using WE43 powder,and characterized the microstructure,mechanical properties,biocompatibility,biodegradation and osteogenic effect of the as-built WE43 porous scaffolds.With the customized energy input and scanning strategy,the relative density of struts reached over 99.5%,and the geometrical error between the designed and the fabricated porosity declined to below 10%.Massive secondary phases including intermetallic precipitates and oxides were observed.The compressive strength(4.37-23.49 MPa)and elastic modulus(154.40-873.02 MPa)were comparable to those of cancellous bone.Good biocompatibility was observed by in vitro cell viability and in vivo implantation.The biodegradation of as-built porous scaffolds promoted the osteogenic effect,but the structural integrity devastated after 12 h by the immersion tests in Hank’s solution and after 4 weeks by the implantation in rabbits’femur,indicating an excessively rapid degradation rate.

pH响应型抗菌纳米材料在抑制口腔菌斑生物膜领域的研究进展

pH响应型抗菌纳米材料是一种可根据机体在生理和病理条件下产生的显著pH差异,选择性地发生结构变化并触发药物释放的新型纳米材料。菌斑内酸性微环境的形成是口腔菌斑生物膜具备致龋性的关键,这也为pH响应型抗菌纳米材料的口腔应用创造了条件。pH响应型抗菌纳米材料能够响应菌斑微环境酸碱度的变化,精准控制抗菌药物的释放,为提高药物效能以及靶向抗菌提供了新方向。本文从pH响应型抗菌纳米材料的分类、作用机制、在抑制口腔菌斑生物膜领域的应用方面进行综述。

Chemical Synthesis of an Octasaccharide Derivative Related to Group B Streptococcus Cell-Wall Polysaccharide

Group B Streptococcus(GBS)is the major pathogen that causes invasive infectious diseases in neonates and infants.The develop-ment of preventive and therapeutic strategies against GBS infection has been becoming the most pressing subject worldwide.Group B carbohydrate(GBC),the group B-specific polysaccharide that distinguishes GBS with other streptococci species,has been identified as an attractive antigen for diagnosis and vaccine development because of its highly conservative tetra-antennary structure.In this paper,a highly convergent[3+5]glycosylation strategy for efficient synthesis of an octasaccharide derivative related to GBC oligo-saccharide unitⅡhas been developed.In this synthesis,each glycosylation reaction was efficiently constructed with glycosyl im-idates,especially trifluoroacetimidate,as donors,and each glycosidic bond was stereoselectively controlled via the neighboring group participation effect of acyl group on the 2-O-position of imidate donors or the solvent effect of Et_(2)O.Furthermore,the ami-noethylphosphate group was smoothly installed on the 6-O-position of D-glucitoll residue using the phosphoramidite method.After global deprotection,the target octasaccharide was successfully obtained from o-glucitol in 29 steps with an overall yield of 1.37%.The free amino group installed on the aminoethylphosphate spacer of the target molecule enables its modification with functional-ized biomolecules for further biological studies.

南海冷水竹节珊瑚的古海洋学应用潜力

Recent deep-diving expeditions in the South China Sea(SCS)discovered abundant indigenous cold-water corals[1].Cold-water corals,preferentially inhabiting the low-latitude intermediate and deep waters with hard substrates and a cool water temperature,have great potential to be applied in researches of the ocean interior by providing high-resolution records[2,3].

以色列阿拉伯人族群身份与国家认同的历史变迁及现实困境

以色列阿拉伯人,是巴勒斯坦地区的阿拉伯原住民,在以色列建国后成为以色列公民,但被犹太复国主义视为潜在安全威胁而受到长期压制与管控。由于以色列的犹太国属性,阿拉伯人遭遇多方面压迫和歧视,无法享有完整的公民权利。2018年以色列《犹太民族国家法》进一步凸显了阿拉伯人的二等公民地位。同时,以色列阿拉伯人是巴勒斯坦人民和阿拉伯民族不可分割的一部分,但不是巴勒斯坦人民的主体部分,在巴勒斯坦民族主义运动中发挥的作用与影响力有限。可见以色列阿拉伯人的集体身份包含以色列人、巴勒斯坦人、阿拉伯人与宗教身份等多重要素。这些认同要素在巴以冲突长期持续的背景下常处于相互矛盾、对立竞争的状态,造成了以色列阿拉伯人的身份焦虑及双重边缘化的认同困境。