Cardiovascular stent restenosis remains a major challenge in interventional treatment of cardiovascular occlusive disease.Although the changes in arterial mechanical environment due to stent implantation are the main ...Cardiovascular stent restenosis remains a major challenge in interventional treatment of cardiovascular occlusive disease.Although the changes in arterial mechanical environment due to stent implantation are the main causes of the initiation of restenosis and thrombosis,the mechanisms that cause this initiation are still not fully understood.In this article,we reviewed the studies on the issue of stent-induced alterations in arterial mechanical environment and discussed their roles in stent restenosis and late thrombosis from three aspects:(i)the interaction of the stent with host blood vessel,involve the response of vascular wall,the mechanism of mechanical signal transmission,the process of re-endothelialization and late thrombosis;(ii)the changes of hemodynamics in the lumen of the vascular segment and(iii)the changes of mechanical microenvironment within the vascular segment wall due to stent implantation.This review has summarized and analyzed current work in order to better solve the two main problems after stent implantation,namely in stent restenosis and late thrombosis,meanwhile propose the deficiencies of current work for future reference.展开更多
Docetaxel(DTX),a paclitaxel analogue,can efficiently inhibit proliferation of vascular smooth muscle cells and has broadly been used as an antiangiogenesis drug.However,as a candidate drug of drug-eluting stent,the ef...Docetaxel(DTX),a paclitaxel analogue,can efficiently inhibit proliferation of vascular smooth muscle cells and has broadly been used as an antiangiogenesis drug.However,as a candidate drug of drug-eluting stent,the effects of DTX on human umbilical vein endothelial cells(HUVECs)are still not well understood.Herein,we investigated the effects of DTX on proliferation,apoptosis,adhesion,migration and morphology of HUVECs in vitro.We found that DTX had the cytostatic and cytotoxic effects at low and high concentrations,respectively.DTX could inhibit the proliferation and migration of HUVECs,induce HUVECs apoptosis,delay HUVECs adhesion and decrease spreading area and aspect ratio of individual cells.The signaling pathway that DTX led to the migration inhibition,adhesion delay and shape change of HUVECs is the VE-cadherin mediated integrin b1/FAK/ROCK signaling pathway.The study will provide a theoretical basis for the clinical application of DTX.展开更多
基金National Natural Science Foundation of China[11332003]National Key R&D Program[2016YFC1102305]+2 种基金Fundamental Research Funds for the Central Universities[106112016CDJXZ238802,106112017CDJZRPY0012 and 106112017CDJZRPY0021]Visiting Scholar Foundation of Key Laboratory of Biorheological Science and Technology of Ministry of Education,Chongqing University[CQKLBST-2016-004 and CQKLBST-2016-010]Chongqing Engineering Laboratory in Vascular Implants and the Public Experiment Center of the State Bioindustrial Base(Chongqing)of China.
文摘Cardiovascular stent restenosis remains a major challenge in interventional treatment of cardiovascular occlusive disease.Although the changes in arterial mechanical environment due to stent implantation are the main causes of the initiation of restenosis and thrombosis,the mechanisms that cause this initiation are still not fully understood.In this article,we reviewed the studies on the issue of stent-induced alterations in arterial mechanical environment and discussed their roles in stent restenosis and late thrombosis from three aspects:(i)the interaction of the stent with host blood vessel,involve the response of vascular wall,the mechanism of mechanical signal transmission,the process of re-endothelialization and late thrombosis;(ii)the changes of hemodynamics in the lumen of the vascular segment and(iii)the changes of mechanical microenvironment within the vascular segment wall due to stent implantation.This review has summarized and analyzed current work in order to better solve the two main problems after stent implantation,namely in stent restenosis and late thrombosis,meanwhile propose the deficiencies of current work for future reference.
基金The study was supported by grants from the National Natural Science Foundation of China(11332003,31370949 and 81400329)the National Key Technology R&D Program of China(2016YFC1102305 and 2012BAI18B02)+2 种基金the Chongqing Graduate Student Research Innovation Project(CYS14023)the Fundamental Research Funds for the Central Universities(106112016CDJXZ238802)as well as the support from the Chongqing Engineering Laboratory in Vascular Implants and the Public Experiment Center of State Bioindustrial Base(Chongqing),China.
文摘Docetaxel(DTX),a paclitaxel analogue,can efficiently inhibit proliferation of vascular smooth muscle cells and has broadly been used as an antiangiogenesis drug.However,as a candidate drug of drug-eluting stent,the effects of DTX on human umbilical vein endothelial cells(HUVECs)are still not well understood.Herein,we investigated the effects of DTX on proliferation,apoptosis,adhesion,migration and morphology of HUVECs in vitro.We found that DTX had the cytostatic and cytotoxic effects at low and high concentrations,respectively.DTX could inhibit the proliferation and migration of HUVECs,induce HUVECs apoptosis,delay HUVECs adhesion and decrease spreading area and aspect ratio of individual cells.The signaling pathway that DTX led to the migration inhibition,adhesion delay and shape change of HUVECs is the VE-cadherin mediated integrin b1/FAK/ROCK signaling pathway.The study will provide a theoretical basis for the clinical application of DTX.