Achieving anti-corrosion and anti-biofouling dual-function self-healing coating by natural carrier attapulgite loading with 2-Undecylimidazoline

In the marine environment,the protective coatings face serious corrosion and biofouling problem,lim-itations,and challenges that made self-healing coatings unable to perform both anti-corrosion and anti-biofouling dual-function at the same time.Here we constructed the corrosion resistance and anti-biofouling self-healing coating by integrating nano-containers into the coating matrix,the 2-Undecylimidazoline(ULM)acted as a corrosion inhibitor and anti-biofouling dual-functional agent which was loaded on the natural container attapulgite(ATP).To obtain high healing efficiency,a fluidity-driven self-healing silicone oil coating was thickened by fibrous ATP to enhance its stability,which played a key role in the self-healing and long-term corrosion resistance.The self-healing time of ULM@ATP rein-forced oil coating was 4 s at least in the air and up to 30 s in the 1 M HCl solution.Meanwhile,the ULM@ATP can significantly enhance the corrosion resistance of the self-healing coating,with the best effect achieved when the content of ULM@ATP was 5 wt.%.The impedance modulus of ULM@ATP-2 still reached 1.62×10^(8)Ωcm^(2) after 480 immersion in 3.5 wt.%NaCl solution,which is 3 orders of magnitude high than pure Oil coating(2.17×10^(5)Ωcm^(2)).The restructure of the ATP network and the release of ULM could largely inhibit the corrosion of metal.The continuous open circuit potential of the compos-ite coating performed the potentially infinite self-healing capacity.The self-healing performances of the composite coating in strong acid and base solutions exhibited high environmental suitability.This anti-corrosion and anti-biofouling(the surface coverage of adhered chlorella decreased 96.88%)dual-function strategy of self-healing coatings could be also realized by many other porous nano-materials or function modifications.The self-healing performances of the composite coating in strong acid and base solutions exhibited high environmental suitability.This anti-corrosion and anti-biofouling dual-function strategy of self-healing coatings could be also realized by many other porous nano-materials or function modifica-tions.The rapid response self-healing coating possessed anti-biofouling and active self-healing functions and showed wide application under more service environments.

Investigation into the epidemiology,genetic characteristics,and clinical manifestations of the first monkeypox outbreak in Shenzhen,China

This paper comprehensively analyses the first-ever monkeypox outbreak in Shenzhen,China,encompassing clinical symptomatology,therapeutic approaches,epidemiological research,and comprehensive laboratory tests,aiming to establish a robust reference for future monkeypox mitigation and management strategies.The investigation involved a thorough investigation of all identified positive cases,including extensive molecular analysis by nucleic acid detection and whole-genome sequencing of the monkeypox virus.Contact tracing and containment of the infected individuals were also undertaken.Three distinct monkeypox cases were identified in this unique outbreak,exhibiting mild and atypical clinical manifestations,primarily fever and rash.All cases were associated with a single transmission chain,primarily facilitated through close contact and homosexual behavior,indicative of a high-risk factor for monkeypox transmission.

MYH7B variants cause hypertrophic cardiomyopathy by activating the Ca MK-signaling pathway

Hypertrophic cardiomyopathy(HCM) is a common genetic disease, predominantly caused by mutations in cardiac sarcomere genes;however, whether MYH7 B causes HCM is not known. In this study, 549 unrelated patients with HCM and 500 healthycontrols were screened using targeted sequencing and whole exome sequencing together. We observed seven variants in MYH7 B causing HCM in 8/549 patients, which accounted for 1.46% of HCM cases. Of these seven variants, three likely pathogenic variants in MYH7 B co-segregating with 5 HCM patients were identified in three HCM pedigrees without other HCM-associated variants. Myh7 b knockout rats were generated and cardiac functions were detected by Millar pressure-volume catheterization and echocardiography. Spontaneous HCM phenotypes, cellular disarray and cardiac fibrosis were observed in both Myh7 b^+/–/Myh7 b^–/–rats. Transcriptome sequencing showed that calcium is the key mediator of cardiac hypertrophy in Myh7 b knockout. Subsequent analysis confirmed over-activation of Ca MK-signaling pathway in cardiomyocytes of Myh7 b^–/–rats.Furthermore, MYH7 B expression in human and rat hearts was identified and micro RNA-208 a and micro RNA-499 levels are unchanged in HCM patients and Myh7 b^+/–/Myh7 b^–/–rats. This study is the first to identify MYH7 B variants as cause of HCM,which account for 1.46% of pathogenesisin HCM patients. Activation of Ca MK-signaling pathway may be involved in its pathophysiology.

Erratum to:MYH7B variants cause hypertrophic cardiomyopathy by activating the CaMK-signaling pathway

In the original manuscript,the value of DBP in WT group was recorded as 128.0±7.6 mm Hg by mistake,which should be the value of SBP in Myh7b;group.And the correct DBP value in WT group should be 88.8±5.3 mm Hg.The corrected Table 3should be as follows.

A novel method for identifying SARS-CoV-2 infection mutants via an epitope-specific CD8^(+)T cell test

Since the outbreak of the coronavirus disease 2019(COVID-19)epidemic in 2019,the public health system has faced enormous challenges.Tracking the individuals who test positive for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a key step for interrupting chains of transmission of SARS-CoV-2 and reducing COVID-19-associated mortality.With the increasing of asymptomatic infections,it is difficult to track asymptomatic infections through epidemiological surveys and virus whole-genome sequencing.However,due to the cross-reactivity of neutralizing antibodies produced by multiple virus subtypes,neutralizing antibody detection cannot be used to determine whether an individual has a history of infection with a specific subtype of SARS-CoV-2.We recruited 4 human leukocyte antigen A2(HLA-A2)infections,15 individuals who received three doses of inactivated vaccines,and 30 breakthrough infections after vaccination and discussed a case-tracking approach to detect epitope-specific CD8^(+)T cells in the peripheral blood of close contacts,including accurate HLA typing based on ribonucleic acid(RNA)-sequencing and flow cytometry data and the comparison and characterization of SARS-CoV-2 HLA-A2 and HLA-A24 epitope-specific CD8^(+)T cells.From individuals who received three doses of inactivated vaccine,we observed that the CD8^(+)T cell specificity for ancestral epitopes was significantly higher than for mutated epitopes,and the fold change of CD8^(+)T cells corresponding to mutated epitopes relative to ancestral epitopes was less than 1.The enzyme-linked immunospot(ELISpot)results further validate this result.This study forms a“method for understanding the infection history of SARS-CoV-2 subtypes based on the proportion of epitope-specific CD8^(+)T cells in the peripheral blood of subjects”,covering up to 46%of the population,including HLA-A2+and HLA-A24+donors,providing a novel method for SARS-CoV-2 infected case tracing.

Early Detection of the Emerging SARS-CoV-2 BA.2.86 Lineage Through Wastewater Surveillance Using a Mediator Probe PCR Assay—Shenzhen City,Guangdong Province,China,2023

Introduction:The emergence of the new severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron sublineage,BA.2.86,has sparked global public health concerns for its potential heightened transmissibility and immune evasion.Utilizing data from Shenzhen’s city-wide wastewater surveillance system,we highlight the presence of the BA.2.86 lineage in Shenzhen.Methods:A mediator probe polymerase chain reaction(PCR)assay was developed to detect the BA.2.86 lineage in wastewater by targeting a specific mutation(Spike:A264D).Between September 19 and December 10,2023,781 wastewater samples from 38 wastewater treatment plants(WWTPs)and 9 pump stations in ten districts of Shenzhen were examined.Through multiple short-amplicon sequencing,three positive samples were identified.Results:The BA.2.86 lineage was identified in the wastewater of Futian and Nanshan districts in Shenzhen on December 2,2023.From December 2 to 10,a total of 21 BA.2.86-positive wastewater samples were found across 6 districts(Futian,Nanshan,Longhua,Baoan,Longgang,and Luohu)in Shenzhen.The weighted average viral load of the BA.2.86 lineage in Shenzhen’s wastewater was 43.5 copies/L on December 2,increased to 219.8 copies/L on December 4,and then decreased to approximately 100 copies/L on December 6,8,and 10.Conclusions:The mediator probe PCR assay,designed for swift detection of low viral concentrations of the BA.2.86 lineage in wastewater samples,shows promise for detecting different SARS-CoV-2 variants.Wastewater surveillance could serve as an early detection system for promptly identifying specific SARS-CoV-2 variants as they emerge.