Cultural heritage is seen as a cultural capital. The heritage tourism increases the local employment and income, however, brings some problems at the same time, changing the traditional living way and industrial struc...Cultural heritage is seen as a cultural capital. The heritage tourism increases the local employment and income, however, brings some problems at the same time, changing the traditional living way and industrial structure. The sustainability of economic development by tourism is also questioned. Fujian Tulou is a world heritage site in China, inscribed in 2008. The nomination has brought great changes to the sites, especially the flourishing tourism industries by the local communities as well as the tourism companies. The latter also take part in the management of the heritage site and get most of the income. The data in economic areas is analyzed in this study and explains the cultural tourism as a positive drive for the local economy, which contributes little to the life of locals. With the analysis of the changes and benefits from the tourism, we have found that tourism, as a dominant industry, might be a potential negative element for heritage conservation. According to the different situations of sites in Fujian Tulou, a proper planning of management is in need to integrate the tourism, conservation and development.展开更多
Background Generally, it is difficult to obtain accurate pose and depth for a non-rigid moving object from a single RGB camera to create augmented reality (AR). In this study, we build an augmented reality system from...Background Generally, it is difficult to obtain accurate pose and depth for a non-rigid moving object from a single RGB camera to create augmented reality (AR). In this study, we build an augmented reality system from a single RGB camera for a non-rigid moving human by accurately computing pose and depth, for which two key tasks are segmentation and monocular Simultaneous Localization and Mapping (SLAM). Most existing monocular SLAM systems are designed for static scenes, while in this AR system, the human body is always moving and non-rigid. Methods In order to make the SLAM system suitable for a moving human, we first segment the rigid part of the human in each frame. A segmented moving body part can be regarded as a static object, and the relative motions between each moving body part and the camera can be considered the motion of the camera. Typical SLAM systems designed for static scenes can then be applied. In the segmentation step of this AR system, we first employ the proposed BowtieNet, which adds the atrous spatial pyramid pooling (ASPP) of DeepLab between the encoder and decoder of SegNet to segment the human in the original frame, and then we use color information to extract the face from the segmented human area. Results Based on the human segmentation results and a monocular SLAM, this system can change the video background and add a virtual object to humans. Conclusions The experiments on the human image segmentation datasets show that BowtieNet obtains state-of-the-art human image segmentation performance and enough speed for real-time segmentation. The experiments on videos show that the proposed AR system can robustly add a virtual object to humans and can accurately change the video background.展开更多
Objective:Acute lung injury(ALI)is characterized by inflammation and currently lacks an efficacious pharmacological intervention.The medicine combination of Lonicerae Japonicae Flos(LJF)and Forsythiae Fructus(FF)demon...Objective:Acute lung injury(ALI)is characterized by inflammation and currently lacks an efficacious pharmacological intervention.The medicine combination of Lonicerae Japonicae Flos(LJF)and Forsythiae Fructus(FF)demonstrates combined properties in its anti-infective,anti-inflammatory,and therapeutic effects,particularly in alleviating respiratory symptoms.In previous studies,Chinese medicine has shown promising efficacy in lipopolysaccharides(LPS)-induced ALI.However,there have been no reports of LJF and FF pairing for lung injury.The aim of this study is to compare the efficacy of herb pair Lonicerae Japonicae Flos-Forsythiae Fructus(LF)with LJF or FF alone in the treatment of ALI,and to explore whether LJF and FF have a combined effect in the treatment of lung injury,along with the underlying mechanism involved.Methods:A total of 36 mice were divided into six groups(control,model,LJF,FF,LF,dexamethasone)based on the treatments they received after undergoing sham-operation/LPS tracheal instillation.H&E staining and pulmonary edema indexes were used to evaluate lung injury severity.Alveolar exudate cells(AECs)were counted based on cell count in bronchoalveolar lavage fluid(BALF),and neutrophil percentage in BALF was measured using flow cytometry.Myeloperoxidase(MPO)activity in BALF was measured using enzyme-linked immunosorbent assay(ELISA),while the production of IL-1β,TNF-α,and IL-6 in the lung and secretion level of them in BALF were detected by quantitative polymerase chain reaction(qPCR)and ELISA.The effect of LJF,FF,and LF on the expression of Caspase-1 and IL-1βproteins in bone marrow derived macrophages(BMDMs)supernatant was assessed using Western blot method under various inflammasome activation conditions.In addition,the concentration of IL-1βand changes in lactatedehydrogenase(LDH)release levels in BMDMs supernatant after LJF,FF,and LF administration,respectively,were measured using ELISA.Furthermore,the effects of LJF,FF and LF on STING and IRF3 phosphorylation in BMDMs were detected by Western blot,and the mRNA changes of IFN-β,TNF-α,IL-6 and CXCL10 in BMDMs were detected by qPCR.Results:LF significantly attenuated the damage to alveolar structures,pulmonary hemorrhage,and infiltration of inflammatory cells induced by LPS.This was evidenced by a decrease in lung index score and wet/dry weight ratio.Treatment with LF significantly reduced the total number of neutrophil infiltration by 75%as well as MPO activity by 88%.The efficacy of LF in reducing inflammatory factors IL-1β,TNF-α,and IL-6 in the lungs surpasses that of LJF or FF,approaching the effectiveness of dexamethasone.In BMDMs,the co-administration of 0.2 mg/mL of LJF and FF demonstrated superior inhibitory effects on the expression of nigericin-stimulated Caspase-1 and IL-1β,as well as the release levels of LDH,compared to individual treatments.Similarly,the combination of 0.5 mg/mL LJF and FF could better inhibit the phosphorylation levels of STING and IRF3 and the production of IFN-β,TNF-α,IL-6,and CXCL10 in response to ISD stimulation.Conclusion:The combination of LJF and FF increases the therapeutic effect on LPS-induced ALI,which may be mechanistically related to the combined effect inhibition of cyclic-GMP-AMP synthase(cGAS)-stimulator of interferon genes(STING)and NOD-like receptor family protein 3(NLRP3)inflammasomes pathways by LJF and FF.Our study provides new medicine candidates for the clinical treatment of ALI.展开更多
Introduction:Raw milk is the basic raw material of dairy products.Bacillus cereus(B.cereus)is a typical conditional pathogenic bacteria and cold-phagocytic spoilage bacteria in raw milk.Materials and Methods:In this s...Introduction:Raw milk is the basic raw material of dairy products.Bacillus cereus(B.cereus)is a typical conditional pathogenic bacteria and cold-phagocytic spoilage bacteria in raw milk.Materials and Methods:In this study,a quantitative polymerase chain reaction(qPCR)method for detecting B.cereus in raw milk was established.The specificity of the method was verified by using other Bacillus bacteria and pathogenic bacteria;the sensitivity of the method was evaluated by preparing recombinant plasmids and simulated contaminated samples;and the applicability of the method was verified using pure spore DNA.The actual sample detection was completed by using the established qPCR method.Results:The qPCR established in this study can specifically detect B.cereus in raw milk.The limit of detection of the method was as low as 200 CFU/mL,the limit of quantification ranged from 2×10^(2)to 2×10^(8)CFU/mL,and the amplification efficiency of qPCR was 96.6%.Conclusions:The method established in this study can distinguish B.cereus from other Bacillus bacteria,and spore DNA can be used as the detection object.This method has the advantages of strong specificity,high sensitivity,wide application range,and short detection time,which isexpectedtobeapplied in thedairy industry.展开更多
Precise control of alloying sites has long been a challenge,yet little has been achieved in the atomic-level manipulation of metallic nanomaterials.This study reported a three-stage metal and motif exchange mechanism ...Precise control of alloying sites has long been a challenge,yet little has been achieved in the atomic-level manipulation of metallic nanomaterials.This study reported a three-stage metal and motif exchange mechanism of alloying reaction of an atomically precise ligand-protected[Ag_(44)(p-MBA)_(30)]^(4−)(p-MBA=para-mercaptobenzoic acid)cluster with[Au_(2)(p-NTP)_(2)Cl]^(−)(p-MBA=para-mercaptobenzoic acid).During the first stage(Stage I),an exchange of ligand-shell metal atoms took place.During the second stage(Stage II),the motif exchanged on the[AuAg_(43)(p-MBA)_(30)]^(4−)cluster.During the third stage(Stage III),the Au(I)atom in the ligand-shell was swapped with a Ag(0)atom of the icosahedral Ag12-core.The density functional theory(DFT)calculation results demonstrated that the metal exchange proceeded via different mechanisms at the different reaction stages.In reaction Stages I and II,the metal exchange proceeded via formation of a dianionic[Ag_(44)(p-MBA)_(30)]^(4−)-[Au_(2)(p-NTP)_(2)Cl]^(−)intermediate and then broke and recombined with the ligand-shell.In Stage III,the diffusion of the Au(I)to icosahedral Ag12-core(Stage III)was proceeded via a motif catalyzed heterometal atom diffusion mechanism.We hope that this work will provide a new perspective for the precise control of alloy position in alloyed nanomaterials.展开更多
Rural heritage is a living heritage of significant importance;it requires community-oriented management for cultural continuity and local development.Different social relations exist at rural heritage sites,whic...Rural heritage is a living heritage of significant importance;it requires community-oriented management for cultural continuity and local development.Different social relations exist at rural heritage sites,which lead to external contestation among stakeholders and internal contestation within the community.It is essential to identify the impact caused by such contestations and determine an appropriate way to negotiate solutions.This paper examines the case of Loushang in Guizhou Province,identifying the contestations by examining the social relations through questionnaires and semi-structured interviews.Negotiation requires an appropriate person or social group to act as intermediary:they engage the stakeholders and induce them to collaborate;they also empower the community in heritage management.The case of Loushang indicates the tools that are necessary to facilitate community-oriented management for rural heritage;it bridges theoretical research and heritage practice.展开更多
基金supported by the Natural Science Foundation of Beijing (7232321)the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine (ZYYCXTD-C-202005)the State Key Program of National Natural Science of China (81930110,82230118)。
文摘Cultural heritage is seen as a cultural capital. The heritage tourism increases the local employment and income, however, brings some problems at the same time, changing the traditional living way and industrial structure. The sustainability of economic development by tourism is also questioned. Fujian Tulou is a world heritage site in China, inscribed in 2008. The nomination has brought great changes to the sites, especially the flourishing tourism industries by the local communities as well as the tourism companies. The latter also take part in the management of the heritage site and get most of the income. The data in economic areas is analyzed in this study and explains the cultural tourism as a positive drive for the local economy, which contributes little to the life of locals. With the analysis of the changes and benefits from the tourism, we have found that tourism, as a dominant industry, might be a potential negative element for heritage conservation. According to the different situations of sites in Fujian Tulou, a proper planning of management is in need to integrate the tourism, conservation and development.
文摘Background Generally, it is difficult to obtain accurate pose and depth for a non-rigid moving object from a single RGB camera to create augmented reality (AR). In this study, we build an augmented reality system from a single RGB camera for a non-rigid moving human by accurately computing pose and depth, for which two key tasks are segmentation and monocular Simultaneous Localization and Mapping (SLAM). Most existing monocular SLAM systems are designed for static scenes, while in this AR system, the human body is always moving and non-rigid. Methods In order to make the SLAM system suitable for a moving human, we first segment the rigid part of the human in each frame. A segmented moving body part can be regarded as a static object, and the relative motions between each moving body part and the camera can be considered the motion of the camera. Typical SLAM systems designed for static scenes can then be applied. In the segmentation step of this AR system, we first employ the proposed BowtieNet, which adds the atrous spatial pyramid pooling (ASPP) of DeepLab between the encoder and decoder of SegNet to segment the human in the original frame, and then we use color information to extract the face from the segmented human area. Results Based on the human segmentation results and a monocular SLAM, this system can change the video background and add a virtual object to humans. Conclusions The experiments on the human image segmentation datasets show that BowtieNet obtains state-of-the-art human image segmentation performance and enough speed for real-time segmentation. The experiments on videos show that the proposed AR system can robustly add a virtual object to humans and can accurately change the video background.
基金granted by the Natural Science Foundation of Beijing,China(Grant No.7232321)Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(No:ZYYCXTD-C-202005)National Natural Science Foundation of China(No.81721002).
文摘Objective:Acute lung injury(ALI)is characterized by inflammation and currently lacks an efficacious pharmacological intervention.The medicine combination of Lonicerae Japonicae Flos(LJF)and Forsythiae Fructus(FF)demonstrates combined properties in its anti-infective,anti-inflammatory,and therapeutic effects,particularly in alleviating respiratory symptoms.In previous studies,Chinese medicine has shown promising efficacy in lipopolysaccharides(LPS)-induced ALI.However,there have been no reports of LJF and FF pairing for lung injury.The aim of this study is to compare the efficacy of herb pair Lonicerae Japonicae Flos-Forsythiae Fructus(LF)with LJF or FF alone in the treatment of ALI,and to explore whether LJF and FF have a combined effect in the treatment of lung injury,along with the underlying mechanism involved.Methods:A total of 36 mice were divided into six groups(control,model,LJF,FF,LF,dexamethasone)based on the treatments they received after undergoing sham-operation/LPS tracheal instillation.H&E staining and pulmonary edema indexes were used to evaluate lung injury severity.Alveolar exudate cells(AECs)were counted based on cell count in bronchoalveolar lavage fluid(BALF),and neutrophil percentage in BALF was measured using flow cytometry.Myeloperoxidase(MPO)activity in BALF was measured using enzyme-linked immunosorbent assay(ELISA),while the production of IL-1β,TNF-α,and IL-6 in the lung and secretion level of them in BALF were detected by quantitative polymerase chain reaction(qPCR)and ELISA.The effect of LJF,FF,and LF on the expression of Caspase-1 and IL-1βproteins in bone marrow derived macrophages(BMDMs)supernatant was assessed using Western blot method under various inflammasome activation conditions.In addition,the concentration of IL-1βand changes in lactatedehydrogenase(LDH)release levels in BMDMs supernatant after LJF,FF,and LF administration,respectively,were measured using ELISA.Furthermore,the effects of LJF,FF and LF on STING and IRF3 phosphorylation in BMDMs were detected by Western blot,and the mRNA changes of IFN-β,TNF-α,IL-6 and CXCL10 in BMDMs were detected by qPCR.Results:LF significantly attenuated the damage to alveolar structures,pulmonary hemorrhage,and infiltration of inflammatory cells induced by LPS.This was evidenced by a decrease in lung index score and wet/dry weight ratio.Treatment with LF significantly reduced the total number of neutrophil infiltration by 75%as well as MPO activity by 88%.The efficacy of LF in reducing inflammatory factors IL-1β,TNF-α,and IL-6 in the lungs surpasses that of LJF or FF,approaching the effectiveness of dexamethasone.In BMDMs,the co-administration of 0.2 mg/mL of LJF and FF demonstrated superior inhibitory effects on the expression of nigericin-stimulated Caspase-1 and IL-1β,as well as the release levels of LDH,compared to individual treatments.Similarly,the combination of 0.5 mg/mL LJF and FF could better inhibit the phosphorylation levels of STING and IRF3 and the production of IFN-β,TNF-α,IL-6,and CXCL10 in response to ISD stimulation.Conclusion:The combination of LJF and FF increases the therapeutic effect on LPS-induced ALI,which may be mechanistically related to the combined effect inhibition of cyclic-GMP-AMP synthase(cGAS)-stimulator of interferon genes(STING)and NOD-like receptor family protein 3(NLRP3)inflammasomes pathways by LJF and FF.Our study provides new medicine candidates for the clinical treatment of ALI.
基金supported by the National Key Research and Development Program of China (2018YFC1604201 and 2018YFC1603800).
文摘Introduction:Raw milk is the basic raw material of dairy products.Bacillus cereus(B.cereus)is a typical conditional pathogenic bacteria and cold-phagocytic spoilage bacteria in raw milk.Materials and Methods:In this study,a quantitative polymerase chain reaction(qPCR)method for detecting B.cereus in raw milk was established.The specificity of the method was verified by using other Bacillus bacteria and pathogenic bacteria;the sensitivity of the method was evaluated by preparing recombinant plasmids and simulated contaminated samples;and the applicability of the method was verified using pure spore DNA.The actual sample detection was completed by using the established qPCR method.Results:The qPCR established in this study can specifically detect B.cereus in raw milk.The limit of detection of the method was as low as 200 CFU/mL,the limit of quantification ranged from 2×10^(2)to 2×10^(8)CFU/mL,and the amplification efficiency of qPCR was 96.6%.Conclusions:The method established in this study can distinguish B.cereus from other Bacillus bacteria,and spore DNA can be used as the detection object.This method has the advantages of strong specificity,high sensitivity,wide application range,and short detection time,which isexpectedtobeapplied in thedairy industry.
基金the National Natural Science Foundation of China(Nos.91961121,21773201,and 21422305)the Project of Innovation Team of the Ministry of Education(No.IRT_17R90).
文摘Precise control of alloying sites has long been a challenge,yet little has been achieved in the atomic-level manipulation of metallic nanomaterials.This study reported a three-stage metal and motif exchange mechanism of alloying reaction of an atomically precise ligand-protected[Ag_(44)(p-MBA)_(30)]^(4−)(p-MBA=para-mercaptobenzoic acid)cluster with[Au_(2)(p-NTP)_(2)Cl]^(−)(p-MBA=para-mercaptobenzoic acid).During the first stage(Stage I),an exchange of ligand-shell metal atoms took place.During the second stage(Stage II),the motif exchanged on the[AuAg_(43)(p-MBA)_(30)]^(4−)cluster.During the third stage(Stage III),the Au(I)atom in the ligand-shell was swapped with a Ag(0)atom of the icosahedral Ag12-core.The density functional theory(DFT)calculation results demonstrated that the metal exchange proceeded via different mechanisms at the different reaction stages.In reaction Stages I and II,the metal exchange proceeded via formation of a dianionic[Ag_(44)(p-MBA)_(30)]^(4−)-[Au_(2)(p-NTP)_(2)Cl]^(−)intermediate and then broke and recombined with the ligand-shell.In Stage III,the diffusion of the Au(I)to icosahedral Ag12-core(Stage III)was proceeded via a motif catalyzed heterometal atom diffusion mechanism.We hope that this work will provide a new perspective for the precise control of alloy position in alloyed nanomaterials.
文摘Rural heritage is a living heritage of significant importance;it requires community-oriented management for cultural continuity and local development.Different social relations exist at rural heritage sites,which lead to external contestation among stakeholders and internal contestation within the community.It is essential to identify the impact caused by such contestations and determine an appropriate way to negotiate solutions.This paper examines the case of Loushang in Guizhou Province,identifying the contestations by examining the social relations through questionnaires and semi-structured interviews.Negotiation requires an appropriate person or social group to act as intermediary:they engage the stakeholders and induce them to collaborate;they also empower the community in heritage management.The case of Loushang indicates the tools that are necessary to facilitate community-oriented management for rural heritage;it bridges theoretical research and heritage practice.
