In recent years,various particulate materials have played important roles in medical applications.However,nano-and micron-sized particles of the same material could exhibit distinct properties due to different particl...In recent years,various particulate materials have played important roles in medical applications.However,nano-and micron-sized particles of the same material could exhibit distinct properties due to different particle sizes.This finding provided a simple and effective way to improve the biological applications of particulate materials.Therefore,as a highly promising member,the effect of the particle size change of the magnesium metal organic framework-74(Mg-MOF74)was well worth evaluating.Here we firsth assessed the in vitro and in vivo toxicity of micron/nanoscale Mg-MOF74(m-Mg-MOF74/n-Mg-MOF74)in detail.Our in vitro study revealed that compared to micron-sized subjects,n-Mg-MOF74 provided a wider range of safe concentrations.Furthermore,both micron/nanoscale Mg-MOF74 showed good biocompatibility and allowed all the rats under the treatment to survive through the expected experimental periods,with n-Mg-MOF74 still showing lower cardiotoxicity.These advantages of nanoscale Mg-MOF74might benefit from its sustainable and balanced release of Ma^2+both inside and outside the cells.Based on the biosafety evaluation,advanced bio-functional assessments of m/n-Mg-MOF74 including early osteogenesis and angiogenesis were alsoperformed.Similarly,the suitable dose groups of n-Mg-MOF74 achieved optimal early osteogenic promotion and angiogenic stimulation effects.Overall,our combined data delineated the toxicity and biological behaviors of Ma-MOF74 of different scales,and sugqested nanoscale Mg-MOF74 as a better choice for future applications.This result revealed that particle size reductior might be a viable strategy to improve and expand medical applications of MOFs or other particulate materials.展开更多
The dysregulation of transcription factors is widely associated with tumorigenesis.As the most well-defined transcription factor in multiple types of cancer,c-Myc can transform cells by transactivating various downstr...The dysregulation of transcription factors is widely associated with tumorigenesis.As the most well-defined transcription factor in multiple types of cancer,c-Myc can transform cells by transactivating various downstream genes.Given that there is no effective way to directly inhibit c-Myc,c-Myc targeting strategies hold great potential for cancer therapy.In this study,we found that WSB1,which has a highly positive correlation with c-Myc in 10 cancer cell lines and clinical samples,is a direct target gene of c-Myc,and can positively regulate c-Myc expression,which forms a feedforward circuit promoting cancer development.RNA sequencing results from Bel-7402 cells confirmed that WSB1 promoted cMyc expression through theβ-catenin pathway.Mechanistically,WSB1 affectedβ-catenin destruction complex-PPP2CA assembly and E3 ubiquitin ligase adaptorβ-TRCP recruitment,which inhibited the ubiquitination ofβ-catenin and transactivated c-Myc.Of interest,the effect of WSB1 on c-Myc was independent of its E3 ligase activity.Moreover,overexpressing WSB1 in the Bel-7402 xenograft model could further strengthen the tumor-driven effect of c-Myc overexpression.Thus,our findings revealed a novel mechanism involved in tumorigenesis in which the WSB1/c-Myc feedforward circuit played an essential role,highlighting a potential c-Myc intervention strategy in cancer treatment.展开更多
基金This study was supported by the National Natural Science Foundation of China(Nos.81601613,81771122,81970985,and 81970984)Key research program of Sichuan Science and technology Department(No.2018SZ0037).
文摘In recent years,various particulate materials have played important roles in medical applications.However,nano-and micron-sized particles of the same material could exhibit distinct properties due to different particle sizes.This finding provided a simple and effective way to improve the biological applications of particulate materials.Therefore,as a highly promising member,the effect of the particle size change of the magnesium metal organic framework-74(Mg-MOF74)was well worth evaluating.Here we firsth assessed the in vitro and in vivo toxicity of micron/nanoscale Mg-MOF74(m-Mg-MOF74/n-Mg-MOF74)in detail.Our in vitro study revealed that compared to micron-sized subjects,n-Mg-MOF74 provided a wider range of safe concentrations.Furthermore,both micron/nanoscale Mg-MOF74 showed good biocompatibility and allowed all the rats under the treatment to survive through the expected experimental periods,with n-Mg-MOF74 still showing lower cardiotoxicity.These advantages of nanoscale Mg-MOF74might benefit from its sustainable and balanced release of Ma^2+both inside and outside the cells.Based on the biosafety evaluation,advanced bio-functional assessments of m/n-Mg-MOF74 including early osteogenesis and angiogenesis were alsoperformed.Similarly,the suitable dose groups of n-Mg-MOF74 achieved optimal early osteogenic promotion and angiogenic stimulation effects.Overall,our combined data delineated the toxicity and biological behaviors of Ma-MOF74 of different scales,and sugqested nanoscale Mg-MOF74 as a better choice for future applications.This result revealed that particle size reductior might be a viable strategy to improve and expand medical applications of MOFs or other particulate materials.
基金supported by grants from Zhejiang Provincial Natural Science Foundation(No.Y18H310001 to Ji Cao,China)the National Natural Science Foundation of China(No.81872885 to Ji Cao+1 种基金No.81625024 to Bo Yang)the Talent Project of Zhejiang Association for Science and Technology(No.2018YCGC002 to Ji Cao,China)。
文摘The dysregulation of transcription factors is widely associated with tumorigenesis.As the most well-defined transcription factor in multiple types of cancer,c-Myc can transform cells by transactivating various downstream genes.Given that there is no effective way to directly inhibit c-Myc,c-Myc targeting strategies hold great potential for cancer therapy.In this study,we found that WSB1,which has a highly positive correlation with c-Myc in 10 cancer cell lines and clinical samples,is a direct target gene of c-Myc,and can positively regulate c-Myc expression,which forms a feedforward circuit promoting cancer development.RNA sequencing results from Bel-7402 cells confirmed that WSB1 promoted cMyc expression through theβ-catenin pathway.Mechanistically,WSB1 affectedβ-catenin destruction complex-PPP2CA assembly and E3 ubiquitin ligase adaptorβ-TRCP recruitment,which inhibited the ubiquitination ofβ-catenin and transactivated c-Myc.Of interest,the effect of WSB1 on c-Myc was independent of its E3 ligase activity.Moreover,overexpressing WSB1 in the Bel-7402 xenograft model could further strengthen the tumor-driven effect of c-Myc overexpression.Thus,our findings revealed a novel mechanism involved in tumorigenesis in which the WSB1/c-Myc feedforward circuit played an essential role,highlighting a potential c-Myc intervention strategy in cancer treatment.