The blob properties in I-mode and ELM-free H-mode plasmas compared to L-mode have been investigated on the EAST tokamak,including the blob detection rate Nb,sizeδb,lifetimeτb and radial velocity vr,b.The blob proper...The blob properties in I-mode and ELM-free H-mode plasmas compared to L-mode have been investigated on the EAST tokamak,including the blob detection rate Nb,sizeδb,lifetimeτb and radial velocity vr,b.The blob properties in L-mode and I-mode are similar,and those in ELM-free H-mode are different to them.The blob Nbis smaller whileτbis larger in H-mode.The experimental blob sizeδband velocity scalings vr,b-δb show a good agreement with the theoretical models.The variation in blob properties during the L-I and H-L transitions,and their relations to the scrape-off layer(SOL)density,edge and SOL turbulence,and SOL collisionality are discussed.The suppression of the edge(inside the last closed flux surface)turbulence is not reflected in the blob behavior,while the blob detection rate shows a correlation with the SOL density and its low-frequency(3–50 kH z)fluctuations.In addition,the blob detection rate is found to increase with the divertor collisionalityΛdiv,indicating a dependence of blob behavior onΛdiv.The differences in blob detection rates among the three operating regimes might be due to their different SOL densities and collisionalities.The investigation contributes to understanding the influences of edge and SOL plasma parameters on the blob behavior.展开更多
To interpret the common symmetric peaks caused by the large-scale structure in the complex S(f)spectrum from the heterodyne Doppler reflectometry(DR)measurement in EAST,a 2D circular-shaped O-mode full-wave model base...To interpret the common symmetric peaks caused by the large-scale structure in the complex S(f)spectrum from the heterodyne Doppler reflectometry(DR)measurement in EAST,a 2D circular-shaped O-mode full-wave model based on the finite-difference time-domain method is built.The scattering characteristics and the influences on the DR signal from various scales are investigated.When the structure is located around the cutoff layer,a moving radial or poloidal large-scale structure k_(θ)k_(θ),match(k_(θ),match is the theoretic wavenumber of Bragg scattering)could both generate an oscillation phase term called‘phase modulation’,and symmetrical peaks in the complex S(f)spectrum.It was found that the image-rejection ratio A_(−1)/A_(+1)(A_(±1)represents the amplitudes of±1 order modulation peaks)could be a feasible indicator for experiment comparison.In the case when the structure is near the cutoff layer with the same arrangement as the experiment for the edge DR channel,the curve of A_(−1)/A_(+1)versus kθcan be divided into three regions,weak asymmetrical range with k_(θ)/k_(0)0.15(k_(0)is the vacuum wavenumber),harmonics range with 0.15k_(θ)/k_(0)0.4,and Bragg scattering range of 0.4k_(θ)/k_(0)0.7.In the case when the structure is located away from the cutoff layer,the final complex S(f)spectrum is the simple superimposing of modulation and Bragg scattering,and the modulation peaks have an amplitude response nearly proportional to the local density fluctuation,called the‘propagationroute effect’.Under the H-mode experiment arrangement for the core DR,a critical fluctuation amplitude Amp(n_(e,Mod.@route))/Amp(n_(e,Tur.@MSA)∼1.3–4.1(Amp(n_(e,Mod.@route))refers to the pedestal large-scale structure amplitude and Amp(ne,Tur.@MSA)refers to turbulence amplitude at the main scattering area)is needed for the structure in the pedestal to be observed by the core DR measurement.The simulations are well consistent with the experimental results.These effects need to be carefully considered during the DR signal analyses as the injecting beam passes through the plasma region with large-scale structures.展开更多
The Doppler reflectometer(DR),a powerful diagnostic for the plasma perpendicular velocity(u⊥)and turbulence measurement,has been widely used in various fusion devices.Many efforts have been put into extracting the Do...The Doppler reflectometer(DR),a powerful diagnostic for the plasma perpendicular velocity(u⊥)and turbulence measurement,has been widely used in various fusion devices.Many efforts have been put into extracting the Doppler shift from the DR signal.There are several commonly used methods for Doppler shift extraction,such as the phase derivative,the center of gravity,and symmetric fitting(SFIT).However,the strong zero-order reflection component around 0 kHz may interfere with the calculation of the Doppler shift.To avoid the influence of the zerofrequency peak,the asymmetric fitting(AFIT)method was designed to calculate the Doppler shift.Nevertheless,the AFIT method may lead to an unacceptable error when the Doppler shift is relatively small compared to the half width at half maximum(HWHM).Therefore,an improved method,which can remove the zero-frequency peak and fit the remaining Doppler peak with a Gaussian function,is devised to extract the Doppler shift.This method can still work reliably whether the HWHM is larger than the Doppler shift or not.展开更多
BACKGROUND: Angelica sinensis is a widely used herb in Chinese traditional medicine. It has been shown to improve hypoxia in embryonic rats and reduce nestin expression in neural stem cells, resulting in proliferatio...BACKGROUND: Angelica sinensis is a widely used herb in Chinese traditional medicine. It has been shown to improve hypoxia in embryonic rats and reduce nestin expression in neural stem cells, resulting in proliferation of neural stem cells. OBJECTIVE: To study the protective effect of Angelica on neural stem cell proliferation in neonatal rats after intrauterine hypoxia. DESIGN, TIME AND SETTING: The randomized, controlled, experiment was performed at the Department of Histology and Embryology, Luzhou Medical College, China from July 2007 to January 2008. MATERIALS: Because gestational days 14-15 are a key stage in rat nervous system development, 21 healthy, pregnant Sprague Dawley rats (14 days after conception) were used for this study. Nestin monoclonal primary antibody was obtained from Chemicon, USA. Angelica parenteral solution (250 g/L) was obtained from Pharmaceutical Preparation Section, Second Affiliated Hospital of Wuhan University, China. METHODS: Rats were randomly divided into a control group (n = 5), a hypoxia group (n = 8), and an Angelica group (n = 8). Saline (8 mL/kg) was injected into the caudal vein of rats in the hypoxia group once a day for seven consecutive days. Intrauterine hypotonic hypoxia was induced using 13% O2 for two hours per day on three consecutive days. Rats in the Angelica group received injections of Angelica parenteral solution (250 g/L); all other protocols were the same as the hypoxia group. The control group procedures were identical to the hypoxia group, but under normal, non-hypoxic conditions. After birth, brain tissues were immediately obtained from neonatal rats and prepared for nestin immunohistochemistry. MAIN OUTCOME MEASURES: Nestin-positive cells in hippocampal CA3 area of neonatal rats in each group were quantified using image analysis to detect signal absorbance. RESULTS: The number of nestin-positive cells increased in the hippocampal CA3 area of neonatal rats in the hypoxia group. The number of nestin-positive cells was less in the Angelica group than in the hypoxia group. Integral absorbance of nestin-positive ceils in the hippocampal CA3 area of neonatal rats was significantly higher in the hypoxia group, compared with the control group (P 〈 0.05). The integral absorbance of nestin positive cells was lower in the Angelica group, compared with the hypoxia group (P 〈 0.05). CONCLUSION: Intrauterine hypoxia, induced for 2 hours daily for three consecutive days, with an oxygen concentration of 13%, stimulated the proliferation of neural stem cells. Angelica injection has a protective effect on neural stem cells from neonatal rats following intrauterine hypoxia by decreasing proliferation of neural stem cells.展开更多
BACKGROUND: The present study analyzed the effect of 3 days (2 h/d) intrauterine hypoxia on learning and memory in juvenile rats, as well as the therapeutic effects of Angelica sinensis on dentate gyrus neurons, as...BACKGROUND: The present study analyzed the effect of 3 days (2 h/d) intrauterine hypoxia on learning and memory in juvenile rats, as well as the therapeutic effects of Angelica sinensis on dentate gyrus neurons, as well as learning and memory. OBJECTIVE: To explore the effects of intrauterine hypoxia on hippocampal dentate gyrus neurons, as well as learning and memory, in juvenile rats; to explore N-methyI-D-aspartate receptor-1 (NMDAR1) expression in the dentate gyrus of neonatal rats following intrauterine hypoxia, as well as prolonged hypoxia; to investigate the regulatory mechanisms of Angelica sinensis. DESIGN, TIME AND SETTING: A randomized and controlled experiment based on developmental neurobiology was performed at the Department of Histology and Embryology in Luzhou Medical College from October 2007 to October 2008. MATERIALS: Angelica sinensis solution (250 g/L) was obtained from Central South Hospital of Wuhan University, China. Neuron-specific enolase and NMDAR1 mRNA in situ hybridization reagents were provided by Wuhan Boster Biological Technology, China. Image-Pro Plus 6.0 analysis system was purchased from Media Cybernetics, USA. METHODS: Healthy pregnant Sprague Dawley rats (n = 30) were randomly divided into control (n = 10), hypoxia (n = 10), and Angelica (n = 10) groups. The Angelica and hypoxia pregnant rats were placed in a three-gas incubator (oxygen concentration: 13%) starting with day 14 of pregnancy for 2 hours/day for 5 consecutive days to establish a fetal rat intrauterine hypoxia model. One hour prior to modeling, the pregnant rats from the Angelica and hypoxia groups received Angelica sinensis and normal saline (8 mL/kg) injections, respectively, through the caudal vein. The control group procedures were identical to the hypoxia group, but lacked the hypoxic conditions. MAIN OUTCOME MEASURES: Brain tissues of neonatal rats were used to detect expression of NMDAR1 mRNA, and brain tissues of juvenile rats aged 30 days were used to determine neuron-specific enolase mRNA expression by in situ hybridization. Microscopic images (400x) of the hippocampal dentate gyrus were collected. The integral optical density (IOD) value of positive NMDAR1 mRNA cells in the dentate gyrus of neonatal rats, as well as the quantity and the IOD value of positive neuron-specific enolase mRNA cells in the dentate gyrus of juvenile rats, were analyzed with Image-Pro IPP6.0 software. At 30 days after birth, learning and memory parameters were measured in the juvenile rats using Morris water maze. RESULTS: The quantity and the IOD value of positive neuron-specific enolase mRNA cells in the dentate gyrus of the hypoxia group juvenile rats were significantly less than the control group (P 〈 0.05), and also less than the Angelica group (P 〈 0.05). The IOD value of positive NMDAR1 mRNA cells in the dentate gyrus of the hypoxia group neonatal rats was significantly greater than the control group, and also greater than the Angelica group (P 〈 0.05). In the Morris water maze, the searching time during the probe trial and reversal probe trial was shorter in the hypoxia group juvenile rats compared with the control group, and the Angelica group was prolonged compared with the hypoxia group (P 〈 0.05). CONCLUSION: Intrauterine hypoxia increased expression of NMDAR1 mRNA in the dentate gyrus of neonatal rats, reduced the number of dentate gyrus neurons, and negatively affected learning and memory in juvenile rats. In contrast, Angelica sinensis injection improved the intrauterine hypoxic condition, increased the number of dentate gyrus neurons, and improved the learning and memory deficits of the juvenile rats.展开更多
An electrostatic Quasi coherent mode has been observed in density fluctuations and perpendicular velocity fluctuations with the frequency range of 3–80 k Hz on the Experimental Advanced Superconducting Tokamak using ...An electrostatic Quasi coherent mode has been observed in density fluctuations and perpendicular velocity fluctuations with the frequency range of 3–80 k Hz on the Experimental Advanced Superconducting Tokamak using multi-channel Doppler reflectometry.It appears in the edge localized mode(ELM)-free period after L-H transition or in the inter-ELM period.The mode rotates almost together with the plasma with the poloidal wave number around 0.6cm-1 and its frequency chirps with plasma poloidal velocity.The mode can exist in a large radial coverage(ρ=0.75–0.98),and peaks near the top of pedestal,suggesting that it might be excited in the steep gradient pedestal region,and spread into the core area.展开更多
Traditional spectral imagers require 2-dimensional detectors. We present a new method to implement spectral imagers with linear detector imager systems based on spectrum compressed. Using 1-dimension detectors instead...Traditional spectral imagers require 2-dimensional detectors. We present a new method to implement spectral imagers with linear detector imager systems based on spectrum compressed. Using 1-dimension detectors instead of 2-dimension detectors to get 3-dimensional data cubes, the spectral imagers could get both the spectral information and the spatial information of each ground object. By the method of characteristics decoupling, we make high precision reconstruction of compressed data. Theoretical analysis and simulations show that it not only ensures the imaging quality but also reduces the dimension of the detectors and complexity of imaging system greatly.展开更多
Circular RNA (circRNA) is a novel type of noncoding RNA that originates from eukaryotic precursor messenger RNA (pre-mRNA).1 These circRNAs regulate tumor processes through diverse mechanisms, including serving as a m...Circular RNA (circRNA) is a novel type of noncoding RNA that originates from eukaryotic precursor messenger RNA (pre-mRNA).1 These circRNAs regulate tumor processes through diverse mechanisms, including serving as a miRNA sponge to modulate expression of miRNA-target genes.2 Eukaryotic translation initiation factor 3 subunit C (eIF3c) is a core subunit of the IF3 complex, involving in the initiation of the translation process of tumor cells.3 Currently, little is known about the underlying molecular mechanisms of circEIF3C in the pathogenesis of intrahepatic cholangiocarcinoma (ICC). A better understanding of the biological functions of circEIF3C in response to tumor cellular growth and how this affects the tumor signaling pathways would provide important clinical value related to the pathogenesis of ICC.展开更多
The p53 tumor suppressor gene is the most frequently mutated gene in cancer. Significant progress has been made to discern the im- portance of p53 in coordinating cellular responses to DNA damage, oncogene activation,...The p53 tumor suppressor gene is the most frequently mutated gene in cancer. Significant progress has been made to discern the im- portance of p53 in coordinating cellular responses to DNA damage, oncogene activation, and other stresses. Noncoding RNAs are RNA molecules functioning without being translated into proteins. In this work, we discuss the dichotomy of p53 regulation by noncoding RNAs with four unconventional questions. First, is overexpression of microRNAs responsible for p53 inactivation in the absence of p53 mutation? Second, are there somatic mutations in the noncoding regions of the p53 gene? Third, is there a germline mutant in the non- coding regions of the p53 gene that predisposes carriers to cancer? Fourth, can p53 activation mediated by a noncoding RNA mutation cause cancer? This work highUghts the prominence of noncoding RNAs in p53 dysregutation and tumorigenesis.展开更多
基金supported by the National Key R&D Program of China(Nos.2022YFE03020004,2017YFE0301300,2018YFE0303104 and 2019YFE03030000)the Major Science and Technology Infrastructure Maintenance and Reconstruction Projects of the Chinese Academy of Sciences+3 种基金National Natural Science Foundation of China(Nos.12275313,11922513,12005004 and U19A20113)the Institute of Energy,Hefei Comprehensive National Science Center(No.GXXT-2020-004)the Anhui Provincial Natural Science Foundation(No.2008085QA38)the Users with Excellence Program of Hefei Science Center,Chinese Academy of Sciences(No.2020HSC-UE009)。
文摘The blob properties in I-mode and ELM-free H-mode plasmas compared to L-mode have been investigated on the EAST tokamak,including the blob detection rate Nb,sizeδb,lifetimeτb and radial velocity vr,b.The blob properties in L-mode and I-mode are similar,and those in ELM-free H-mode are different to them.The blob Nbis smaller whileτbis larger in H-mode.The experimental blob sizeδband velocity scalings vr,b-δb show a good agreement with the theoretical models.The variation in blob properties during the L-I and H-L transitions,and their relations to the scrape-off layer(SOL)density,edge and SOL turbulence,and SOL collisionality are discussed.The suppression of the edge(inside the last closed flux surface)turbulence is not reflected in the blob behavior,while the blob detection rate shows a correlation with the SOL density and its low-frequency(3–50 kH z)fluctuations.In addition,the blob detection rate is found to increase with the divertor collisionalityΛdiv,indicating a dependence of blob behavior onΛdiv.The differences in blob detection rates among the three operating regimes might be due to their different SOL densities and collisionalities.The investigation contributes to understanding the influences of edge and SOL plasma parameters on the blob behavior.
基金supported by Shenzhen Clean Energy Research Institute,the National MCF Energy R&D Program of China(Nos.2018YFE0311200 and 2022YFE03070004)National Natural Science Foundation of China(Nos.U1967206 and 12075155)+1 种基金the China Postdoctoral Science Foundation(No.2021M702270)the Fundamental Research Funds for the Central Universities(No.WK3420000018)。
文摘To interpret the common symmetric peaks caused by the large-scale structure in the complex S(f)spectrum from the heterodyne Doppler reflectometry(DR)measurement in EAST,a 2D circular-shaped O-mode full-wave model based on the finite-difference time-domain method is built.The scattering characteristics and the influences on the DR signal from various scales are investigated.When the structure is located around the cutoff layer,a moving radial or poloidal large-scale structure k_(θ)k_(θ),match(k_(θ),match is the theoretic wavenumber of Bragg scattering)could both generate an oscillation phase term called‘phase modulation’,and symmetrical peaks in the complex S(f)spectrum.It was found that the image-rejection ratio A_(−1)/A_(+1)(A_(±1)represents the amplitudes of±1 order modulation peaks)could be a feasible indicator for experiment comparison.In the case when the structure is near the cutoff layer with the same arrangement as the experiment for the edge DR channel,the curve of A_(−1)/A_(+1)versus kθcan be divided into three regions,weak asymmetrical range with k_(θ)/k_(0)0.15(k_(0)is the vacuum wavenumber),harmonics range with 0.15k_(θ)/k_(0)0.4,and Bragg scattering range of 0.4k_(θ)/k_(0)0.7.In the case when the structure is located away from the cutoff layer,the final complex S(f)spectrum is the simple superimposing of modulation and Bragg scattering,and the modulation peaks have an amplitude response nearly proportional to the local density fluctuation,called the‘propagationroute effect’.Under the H-mode experiment arrangement for the core DR,a critical fluctuation amplitude Amp(n_(e,Mod.@route))/Amp(n_(e,Tur.@MSA)∼1.3–4.1(Amp(n_(e,Mod.@route))refers to the pedestal large-scale structure amplitude and Amp(ne,Tur.@MSA)refers to turbulence amplitude at the main scattering area)is needed for the structure in the pedestal to be observed by the core DR measurement.The simulations are well consistent with the experimental results.These effects need to be carefully considered during the DR signal analyses as the injecting beam passes through the plasma region with large-scale structures.
基金supported in part by the National MCF Energy R&D Program(Nos.2018YFE0311200 and 2017YFE0301204)National Natural Science Foundation of China(Nos.U1967206,11975231 and 11922513)supported by the Users with Excellence Program of Hefei Science Center CAS(No.2020HSC-UE009).
文摘The Doppler reflectometer(DR),a powerful diagnostic for the plasma perpendicular velocity(u⊥)and turbulence measurement,has been widely used in various fusion devices.Many efforts have been put into extracting the Doppler shift from the DR signal.There are several commonly used methods for Doppler shift extraction,such as the phase derivative,the center of gravity,and symmetric fitting(SFIT).However,the strong zero-order reflection component around 0 kHz may interfere with the calculation of the Doppler shift.To avoid the influence of the zerofrequency peak,the asymmetric fitting(AFIT)method was designed to calculate the Doppler shift.Nevertheless,the AFIT method may lead to an unacceptable error when the Doppler shift is relatively small compared to the half width at half maximum(HWHM).Therefore,an improved method,which can remove the zero-frequency peak and fit the remaining Doppler peak with a Gaussian function,is devised to extract the Doppler shift.This method can still work reliably whether the HWHM is larger than the Doppler shift or not.
基金the founda-tion of Sichuan Provincial Department of Science and Technology, No. 05JY029-103-1the foundation of Sichuan Provincial Health Department, No. 060052
文摘BACKGROUND: Angelica sinensis is a widely used herb in Chinese traditional medicine. It has been shown to improve hypoxia in embryonic rats and reduce nestin expression in neural stem cells, resulting in proliferation of neural stem cells. OBJECTIVE: To study the protective effect of Angelica on neural stem cell proliferation in neonatal rats after intrauterine hypoxia. DESIGN, TIME AND SETTING: The randomized, controlled, experiment was performed at the Department of Histology and Embryology, Luzhou Medical College, China from July 2007 to January 2008. MATERIALS: Because gestational days 14-15 are a key stage in rat nervous system development, 21 healthy, pregnant Sprague Dawley rats (14 days after conception) were used for this study. Nestin monoclonal primary antibody was obtained from Chemicon, USA. Angelica parenteral solution (250 g/L) was obtained from Pharmaceutical Preparation Section, Second Affiliated Hospital of Wuhan University, China. METHODS: Rats were randomly divided into a control group (n = 5), a hypoxia group (n = 8), and an Angelica group (n = 8). Saline (8 mL/kg) was injected into the caudal vein of rats in the hypoxia group once a day for seven consecutive days. Intrauterine hypotonic hypoxia was induced using 13% O2 for two hours per day on three consecutive days. Rats in the Angelica group received injections of Angelica parenteral solution (250 g/L); all other protocols were the same as the hypoxia group. The control group procedures were identical to the hypoxia group, but under normal, non-hypoxic conditions. After birth, brain tissues were immediately obtained from neonatal rats and prepared for nestin immunohistochemistry. MAIN OUTCOME MEASURES: Nestin-positive cells in hippocampal CA3 area of neonatal rats in each group were quantified using image analysis to detect signal absorbance. RESULTS: The number of nestin-positive cells increased in the hippocampal CA3 area of neonatal rats in the hypoxia group. The number of nestin-positive cells was less in the Angelica group than in the hypoxia group. Integral absorbance of nestin-positive ceils in the hippocampal CA3 area of neonatal rats was significantly higher in the hypoxia group, compared with the control group (P 〈 0.05). The integral absorbance of nestin positive cells was lower in the Angelica group, compared with the hypoxia group (P 〈 0.05). CONCLUSION: Intrauterine hypoxia, induced for 2 hours daily for three consecutive days, with an oxygen concentration of 13%, stimulated the proliferation of neural stem cells. Angelica injection has a protective effect on neural stem cells from neonatal rats following intrauterine hypoxia by decreasing proliferation of neural stem cells.
基金Supported by:a grant by Sichuan Provincial Science and Technology Bureau,No. 05JY029-103:a grant by Sichuan Provincial Education Bureau.No.2006A152
文摘BACKGROUND: The present study analyzed the effect of 3 days (2 h/d) intrauterine hypoxia on learning and memory in juvenile rats, as well as the therapeutic effects of Angelica sinensis on dentate gyrus neurons, as well as learning and memory. OBJECTIVE: To explore the effects of intrauterine hypoxia on hippocampal dentate gyrus neurons, as well as learning and memory, in juvenile rats; to explore N-methyI-D-aspartate receptor-1 (NMDAR1) expression in the dentate gyrus of neonatal rats following intrauterine hypoxia, as well as prolonged hypoxia; to investigate the regulatory mechanisms of Angelica sinensis. DESIGN, TIME AND SETTING: A randomized and controlled experiment based on developmental neurobiology was performed at the Department of Histology and Embryology in Luzhou Medical College from October 2007 to October 2008. MATERIALS: Angelica sinensis solution (250 g/L) was obtained from Central South Hospital of Wuhan University, China. Neuron-specific enolase and NMDAR1 mRNA in situ hybridization reagents were provided by Wuhan Boster Biological Technology, China. Image-Pro Plus 6.0 analysis system was purchased from Media Cybernetics, USA. METHODS: Healthy pregnant Sprague Dawley rats (n = 30) were randomly divided into control (n = 10), hypoxia (n = 10), and Angelica (n = 10) groups. The Angelica and hypoxia pregnant rats were placed in a three-gas incubator (oxygen concentration: 13%) starting with day 14 of pregnancy for 2 hours/day for 5 consecutive days to establish a fetal rat intrauterine hypoxia model. One hour prior to modeling, the pregnant rats from the Angelica and hypoxia groups received Angelica sinensis and normal saline (8 mL/kg) injections, respectively, through the caudal vein. The control group procedures were identical to the hypoxia group, but lacked the hypoxic conditions. MAIN OUTCOME MEASURES: Brain tissues of neonatal rats were used to detect expression of NMDAR1 mRNA, and brain tissues of juvenile rats aged 30 days were used to determine neuron-specific enolase mRNA expression by in situ hybridization. Microscopic images (400x) of the hippocampal dentate gyrus were collected. The integral optical density (IOD) value of positive NMDAR1 mRNA cells in the dentate gyrus of neonatal rats, as well as the quantity and the IOD value of positive neuron-specific enolase mRNA cells in the dentate gyrus of juvenile rats, were analyzed with Image-Pro IPP6.0 software. At 30 days after birth, learning and memory parameters were measured in the juvenile rats using Morris water maze. RESULTS: The quantity and the IOD value of positive neuron-specific enolase mRNA cells in the dentate gyrus of the hypoxia group juvenile rats were significantly less than the control group (P 〈 0.05), and also less than the Angelica group (P 〈 0.05). The IOD value of positive NMDAR1 mRNA cells in the dentate gyrus of the hypoxia group neonatal rats was significantly greater than the control group, and also greater than the Angelica group (P 〈 0.05). In the Morris water maze, the searching time during the probe trial and reversal probe trial was shorter in the hypoxia group juvenile rats compared with the control group, and the Angelica group was prolonged compared with the hypoxia group (P 〈 0.05). CONCLUSION: Intrauterine hypoxia increased expression of NMDAR1 mRNA in the dentate gyrus of neonatal rats, reduced the number of dentate gyrus neurons, and negatively affected learning and memory in juvenile rats. In contrast, Angelica sinensis injection improved the intrauterine hypoxic condition, increased the number of dentate gyrus neurons, and improved the learning and memory deficits of the juvenile rats.
基金supported in part by the National MCF Energy R&D Program of China(Nos.2018YFE0311200,2017YFE0301204 and 2017YFE0301700)National Natural Science Foundation of China(Nos.11635008,U1967206 and 11975231)。
文摘An electrostatic Quasi coherent mode has been observed in density fluctuations and perpendicular velocity fluctuations with the frequency range of 3–80 k Hz on the Experimental Advanced Superconducting Tokamak using multi-channel Doppler reflectometry.It appears in the edge localized mode(ELM)-free period after L-H transition or in the inter-ELM period.The mode rotates almost together with the plasma with the poloidal wave number around 0.6cm-1 and its frequency chirps with plasma poloidal velocity.The mode can exist in a large radial coverage(ρ=0.75–0.98),and peaks near the top of pedestal,suggesting that it might be excited in the steep gradient pedestal region,and spread into the core area.
文摘Traditional spectral imagers require 2-dimensional detectors. We present a new method to implement spectral imagers with linear detector imager systems based on spectrum compressed. Using 1-dimension detectors instead of 2-dimension detectors to get 3-dimensional data cubes, the spectral imagers could get both the spectral information and the spatial information of each ground object. By the method of characteristics decoupling, we make high precision reconstruction of compressed data. Theoretical analysis and simulations show that it not only ensures the imaging quality but also reduces the dimension of the detectors and complexity of imaging system greatly.
文摘Circular RNA (circRNA) is a novel type of noncoding RNA that originates from eukaryotic precursor messenger RNA (pre-mRNA).1 These circRNAs regulate tumor processes through diverse mechanisms, including serving as a miRNA sponge to modulate expression of miRNA-target genes.2 Eukaryotic translation initiation factor 3 subunit C (eIF3c) is a core subunit of the IF3 complex, involving in the initiation of the translation process of tumor cells.3 Currently, little is known about the underlying molecular mechanisms of circEIF3C in the pathogenesis of intrahepatic cholangiocarcinoma (ICC). A better understanding of the biological functions of circEIF3C in response to tumor cellular growth and how this affects the tumor signaling pathways would provide important clinical value related to the pathogenesis of ICC.
文摘The p53 tumor suppressor gene is the most frequently mutated gene in cancer. Significant progress has been made to discern the im- portance of p53 in coordinating cellular responses to DNA damage, oncogene activation, and other stresses. Noncoding RNAs are RNA molecules functioning without being translated into proteins. In this work, we discuss the dichotomy of p53 regulation by noncoding RNAs with four unconventional questions. First, is overexpression of microRNAs responsible for p53 inactivation in the absence of p53 mutation? Second, are there somatic mutations in the noncoding regions of the p53 gene? Third, is there a germline mutant in the non- coding regions of the p53 gene that predisposes carriers to cancer? Fourth, can p53 activation mediated by a noncoding RNA mutation cause cancer? This work highUghts the prominence of noncoding RNAs in p53 dysregutation and tumorigenesis.