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Removal of ammonium and nitrate through Anammox and FeS-driven autotrophic denitrification
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作者 Yanfei Wang xiaona zheng +1 位作者 Guangxue Wu Yuntao Guan 《Frontiers of Environmental Science & Engineering》 SCIE EI CSCD 2023年第6期127-139,共13页
An autotrophic denitrifying bioreactor with iron sulfide(FeS)as the electron donor was operated to remove ammonium(NH_(4)^(+))and nitrate(NO_(3)^(-))synergistically from wastewater for more than 298 d.The concentratio... An autotrophic denitrifying bioreactor with iron sulfide(FeS)as the electron donor was operated to remove ammonium(NH_(4)^(+))and nitrate(NO_(3)^(-))synergistically from wastewater for more than 298 d.The concentration of FeS greatly affected the removal of NH_(4)^(+)/NO_(3)^(-).Additionally,a low hydraulic retention time worsened the removal efficiency of NH_(4)^(+)/NO_(3)^(-).When the hydraulic retention time was 12 h,the optimal removal was achieved with NH_(4)^(+)and NO_(3)^(-)removal percentages both above 88%,and the corresponding nitrogen removal loading rates of NH_(4)^(+)and NO_(3)^(-)were 49.1 and 44.0 mg/(L·d),respectively.The removal of NH_(4)^(+)mainly occurred in the bottom section of the bioreactor through sulfate/ferric reducing anaerobic ammonium oxidation(Sulfammox/Feammox),nitrification,and anaerobic ammonium oxidation(Anammox)by functional microbes such as Nitrospira,Nitrosomonas,and Candidatus Kuenenia.Meanwhile,NO_(3)^(-)was mainly removed in the middle and upper sections of the bioreactor through autotrophic denitrification by Ferritrophicum,Thiobacillus,Rhodanobacter,and Pseudomonas,which possessed complete denitrification-related genes with high relative abundances. 展开更多
关键词 ANAMMOX DENITRIFICATION FES NH_(4)^(+)/NO_(3)^(−) Sulfammox
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Embryonic lineage tracing with Procr-CreER marks balanced hematopoietic stem cell fate during entire mouse lifespan 被引量:1
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作者 xiaona zheng Guangyu Zhang +7 位作者 Yandong Gong Xiaowei Ning Zhijie Bai Jian He Fan Zhou Yanli Ni Yu Lan Bing Liu 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2019年第10期489-498,共10页
The functional heterogeneity of hematopoietic stem cells(HSCs) has been comprehensively investigated by single-cell transplantation assay.However,the heterogeneity regarding their physiological contribution remains an... The functional heterogeneity of hematopoietic stem cells(HSCs) has been comprehensively investigated by single-cell transplantation assay.However,the heterogeneity regarding their physiological contribution remains an open question,especially for those with life-long hematopoietic fate of rigorous selfrenewing and balanced differentiation capacities.In this study,we revealed that Procr expression was detected principally in phenotypical vascular endothelium co-expressing DII4 and CD44 in the midgestation mouse embryos,and could enrich all the HSCs of the embryonic day 11.5(E11.5) aortagonad-mesonephros(AGM) region.We then used a temporally restricted genetic tracing strategy to irreversibly label the Procr-exp res sing cells at E9.5.Interestingly,most labeled mature HSCs in multiple sites(such as AGM) around E11.5 were functionally categorized as lymphomyeloid-balanced HSCs assessed by direct transplantation.Furthermore,the labeled cells contributed to an average of 7.8% of immunophenotypically defined HSCs in E14.5 fetal liver(FL) and 6.9% of leukocytes in peripheral blood(PB) during one-year follow-up.Surprisingly,in aged mice of 24 months,the embryonically tagged cells displayed constant contribution to leukocytes with no bias to myeloid or lymphoid lineages.Altogether,we demonstrated,for the first time,the existence of a subtype of physiologically long-lived balanced HSCs as hypothesized,whose precise embryonic origin and molecular identity await further characterization. 展开更多
关键词 Genetic lineage tracing Hematopoietic stem cells Procr HETEROGENEITY Aorta-gonad-mesonephros region
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Single-cell transcriptomic profiling of non-hematopoietic circulating cells in mid-gestational mouse embryos
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作者 Yanyan Li Yanjuan Chen +7 位作者 xiaona zheng Yun Gao Yuxuan zheng Zongcheng Li Han He Fuchou Tang Bing Liu Yu Lan 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2021年第6期508-511,共4页
The circulatory system is indispensable for an animal’s normal physiology.In addition to well-characterized hematopoietic cells,several types of circulating non-hematopoietic cells have been identified in adult mice(... The circulatory system is indispensable for an animal’s normal physiology.In addition to well-characterized hematopoietic cells,several types of circulating non-hematopoietic cells have been identified in adult mice(Zhang and Huang,2012;Schreier and Triampo,2020). 展开更多
关键词 HEMATOPOIETIC GESTATIONAL circulating
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