四聚体技术检测人外周血巨细胞病毒抗原特异性CTL

为深入了解抗病毒免疫机制,探索人在健康状态及巨细胞病毒(CMV)急性感染时抗原特异性细胞毒T淋巴细胞(CTL)数量的动态变化。以CMV抗原肽、HLA-A*0201重链和轻链制备CMV四聚体;分离并检测12名健康被检者外周血单个核细胞(PBMC)中CMV抗原特异CTL数量;PBMC体外培养建立CTL细胞系,于末次刺激的不同时相进行四聚体染色,流式细胞仪(FACS)检测抗原特异CTL数量。结果发现9名被检者PBMC中均检出抗原特异CTL;细胞系中CMV特异性CTL数量急剧增多,细胞系与PBMC相比,差异有显著的统计学意义(P<0.001);研究结果提示,9名被检者感染过CMV,血液中存在少量免疫记忆性T细胞,当再次遭遇同一抗原后发生克隆扩增。

HIV-Associated Pathogenesis: The Role of HIV-1 Nef Gene

Since the discovery of HIV more than two decades ago,scientific progress has been impressive and has dramatically advanced our understanding of HIV infection,from molecular through cellular and then on to systemic pathogenesis,such as the functions of HIV-1 nef gene.However,there is still much to be learned before we fully understand how the host interacts with the virus at molecular level and how immune responses correlate with protection from disease.This review describes our current knowledge of HIV/AIDS with the reference of HIV nef functions and its role in HIV-1 mediated pathogenesis.

On the Infinite-Dimensional Modular Lie Superalgebras of O-Type

The natural filtrations of infinite-dimensional Lie superalgebras of O-type over a field of positive characteristic are proved to be invariant under automorphisms by studying ad-nilpotent elements.We observe that two Lie superalgebras of O-type are isomorphic if and only if the integers in the definition of these Lie superalgebras are intrinsic.Thereby,all the Lie superalgebras of O-type are classified in the sense of isomorphism.

Autocatalytic strategy for tuning drug release from peptide-drug supramolecular hydrogel

Peptide-drug conjugates(PDCs) composed of peptide, spacer and drug have gained extensive attention in the field of drug delivery owing to its precise control over the drug payload and architecture. However,the achievement of controllable and rapid drug release at targeted site by PDCs is still a great challenge for pharmaceutist. Herein, we introduced the histidine residue into PDCs to generate a supramolecular hydrogel via a p H-trigger strategy, which exhibited an autocatalytic effect to precisely tune drug release from PDCs hydrogel. Using indomethacin(Idm) as model drug, various PDCs(Y(Idm)EEH, Y(Idm)EEK and Y(Idm)EER) were synthesized and their self-assembling properties were investigated in terms of critical aggregation concentration(CAC), transmission electron microscopy(TEM) and rheometer. Introduction of histidine residue into PDCs presented a robust catalytic activity on the ester hydrolysis of p-nitrophenyl acetate in aqueous solution, as well conferred the autocatalytic capacity to hydrolyze the PDCs into active parent drug(Idm). Overall, we reported an autocatalytic activity of histidine residue to precisely tune drug release from PDCs hydrogels.

Viral and Antibody Kinetics of COVID-19 Patients with Different Disease Severities in Acute and Convalescent Phases:A 6-Month Follow-Up Study

Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has spread rapidly around the world,posing a major threat to human health and the economy.Currently,long-term data on viral shedding and the serum antibody responses in COVID-19 patients are still limited.Herein,we report the clinical features,viral RNA loads,and serum antibody levels in a cohort of 112 COVID-19 patients admitted to the Honghu People’s Hospital,Hubei Province,China.Overall,5.36%(6/112)of patients showed persistent viral RNA shedding(>45 days).The peak viral load was higher in the severe disease group than in the mild group(median cycle threshold value,36.4 versus 31.5;P=0.002).For most patients the disappearance of IgM antibodies occurred approximately 4–6 weeks after symptoms onset,while IgG persisted for over 194 days after the onset of symptoms,although patients showed a 46%reduction in antibodies titres against SARS-CoV-2 nucleocapsid protein compared with the acute phase.We also studied18 asymptomatic individuals with RT-qPCR confirmed SARS-CoV-2 infection together with 17 symptomatic patients,and the asymptomatic individuals were the close contacts of these symptomatic cases.Delayed IgG seroconversion and lower IgM seropositive rates were observed in asymptomatic individuals.These data indicate that higher viral loads and stronger antibody responses are related to more severe disease status in patients with SARS-CoV-2 infection,and the antibodies persisted in the recovered patient for more than 6 months so that the vaccine may provide protection against SARS-CoV-2 infection.

Some Subsystems of a Lie Triple System Closely Related to Its Frattini Subsystem

The main purpose of the present paper is to give some properties of the Jacobson radical, the Frattini subsystem and c-ideals of a Lie triple system. Some further results concerning the Frattini subsystems of nilpotent and solvable Lie triple systems are obtained. Moreover, we develop inititally c-ideals for a Lie triple system and make use of them to give some characterizations of a solvable Lie triple system.

Superderivation Algebras of Modular Lie Superalgebras of ■-Type

The authors consider a family of finite-dimensional Lie superalgebras of C-type over an algebraically closed field of characteristic p 〉 3. It is proved that the Lie superalgebras of C-type are simple and the spanning sets are determined. Then the spanning sets are employed to characterize the superderivation algebras of these Lie superalgebras. Finally, the associative forms are discussed and a comparison is made between these Lie superalgebras and other simple Lie superalgebras of Cartan type.

The Even Part of Finite-Dimensional Modular Lie Superalgebra Г

Let F be the underlying base field of characteristic p>3 and denote by Гo the even part of the finite-dimensional Lie superalgebra T.We give the generator sets of the Lie algebra Гo.Using certain properties of the canonical tori of Гo,we describe explicitly the derivation algebra of Гo.