Meta-analysis and Trial Sequential Analysis of Compound Qingdai Capsule in the Treatment of Psoriasis

[Objectives]To analyze the efficacy of Compound Qingdai Capsule in the treatment of psoriasis and conduct a systematic evaluation.[Methods]The clinical total effective rate,PASI index score,IL-17 level,IL-23 level,TNF-level,and adverse reactions were analyzed.TSA 0.9 software was used to conduct sequential analysis of the total effective rate,and subgroup analysis was performed according to the average age of the experimental group.[Results]Single application of Compound Qingdai Capsule or in combination with other methods in the treatment of psoriasis was superior to non-Compound Qingdai Capsule group,and the side effects were less than non-Compound Qingdai Capsule group;the n≥40 year-old group had certain heterogeneity,suggesting that the difference was statistically significant,and the effective rate was higher than that of the control group.The funnel plot showed that the graph was asymmetrical,and there may be publication bias or the possibility of low-quality literature.The TSA results indicated that the actual sample size was far lower than the expected sample size,and the cumulative Z value did not reach the TSA cut-off value and more trials need to be included to confirm the efficacy.[Conclusions]Compound Qingdai Capsule has a clear curative effect on psoriasis,and its safety is high.This study can provide relevant evidence for the effectiveness of Chinese patent drugs(CPD)in treating psoriasis.

The expression of P63 protein in some keratinocyte original tissues and cells

Objective： To examine the expression patterns of p63 in tissues of particular keratinocyte original hyperproliferate diseases and variety cell types for determining if P63 is the marker of proliferative potential keratinocytes. Methods： P63 protein was detected and analyzed by immunoreactivity method and Western blot in biopsy specimens of keratinocyte original disorders including squamous cell carcinomas SCC, basal cell carcinomas BCC, Bowen＇ s disease and other tissues or cells, such as psoriasis vulgaris, normal skin tissues, primary cultured keratinocytes, immortal HaCaT cells, and epidermoid carcinoma cells A431. Results： P63 protein was expressed in the nuclei of basal and suprabasal layer of the epidermis, germinative cells of sebaceous glands in normal epidermal. P63 was strongly and diffusely detected in the majority of tumor cells in BCC and poorly-differentiated SCC. In Bowen＇ s disease, p63 expresses are remarkable in all cell layers. In the psoriasis plaque epidermal, p63 expressed mainly in basal cells and part of spinous cells. P63 expressed more strongly in primary cultured keratinocytes than in A431 cells or HaCaT cells. Conclusion： P63 is a nuclei marker of undifferentiated keratinocytes with the proliferative potential and may disrupt the terminal differentiation. The overexpression of p63 reflects immaturity of the tumor cells. The immunohistochemical staining of p63 may be useful for investigating the origin and differentiation of tumor cells.

Evaluating the Impact of Trichoderma brevicompactum 31636 on Root Rot of Atractylodes macrocephala and the Fungal Community in the Rhizosphere Soil

Background:Trichoderma brevicompactum can control of plant diseases,but there are few studies about the control of root rot by T.brevicompactum 31636 and its biocontrol mechanism.Objective:To explore the impact of T.brevicompactum 31636 on root rot and the structure of fungal communities of rhizosphere soil.Methods:The effect of T.brevicompactum 31636 on controlling root rot was assessed,the growth of plants was observed by pot experiments,and the fungal structure was detected by high-throughput sequencing analysis.Results:Compared with the CK(healthy plants)group,the plant height,fresh weight,and dry weight increased by 55.3%,48.55%and 57.8%,respectively.While the concentration of atractylenolideⅠ,Ⅱ,Ⅲ,and atractylone increased by 19.35%,16.96%,89.42%and 89.32%,respectively.The results of pot experiments indicated that T.brevicompactum 31636 could significantly improve the survival rate of Atractylodes macrocephala.The results of principal coordinates analysis(PCoA)showed that the sample of CK and Fo+Th(adding Fusdrium oxysporum before T.brevicompactum 31636)group on one side,and samples of Th and Fo group on the other.Furthermore,among the top 20 dominant fungi detected,the fungal genus with 1%increase of relative abundance included Trichoderma(1%),Sordariomycetes(1.5%),Mortierella(1.3%),Penicillium(1%),meanwhile the fungal genus with 1%decrease of relative abundance included Fusarium(30%)and Pleosporales(1%).Conclusion:In summary,T.brevicompactum 31636 can control the root rot of A.macrocephala by regulating of the soil fungal community and inhibiting pathogen.