Dispase rapidly and effectively purifies Schwann cells from newborn mice and adult rats

In the present study, Schwann cells were isolated from the sciatic nerve of neonatal mice and purified using dispase and collagenase. Results showed that after the first round of purification with dispase, most of the Schwann cells appeared round in shape and floated in culture solution after 15 minutes. In addition, cell yield and cell purity were higher when compared to the collagenase group. After the second round of purification, the final cell yield for the dispase group was higher than that for the collagenase group, but no significant difference was found in cell purity. Moreover, similar results in cell quantity and purity were observed in adult Sprague-Dawley rats. These findings indicate that purification with dispase can result in the rapid isolation of Schwann cells with a high yield and purity.

中毒患者急诊护理专家共识(2024版)

中毒会危及患者生命,因其整体发病率不高、不被广大医护熟悉,给急诊医护人员带来许多挑战。有必要对中毒患者采取有条理、深思熟虑的治疗方法。了解毒物学病史和体格检查的细微差别,规范接诊中毒患者的首次接触,有助于管理这些病人。为此,湖北省中毒与职业病联盟组织国内多家医疗机构,综合相关专家的学术和临床经验起草本专家共识,并和部分中毒救治经验丰富的护理专家实战运用,最后提交本共识专家委员会讨论通过,就中毒患者急诊护理达成共识。本文旨在帮助急诊护理人员对中毒患者做出实用可行的评估与管理。