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Prevalence and associated factors of myocardial involvement in Duchenne muscular dystrophy patients in the first decade of life
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作者 Rong Xu Huayan Xu +13 位作者 Kun Zhang Hong Xu Hui Liu Hang Fu Linjun Xie Ke Xu Chuan Fu Xuesheng Li Xiaoyue Zhou Rajiv Ananthakrishna Joseph BSelvanayagam Li Yu xiaotang cai Yingkun Guo 《Chinese Medical Journal》 SCIE CAS CSCD 2023年第9期1132-1134,共3页
To the Editor:Duchenne muscular dystrophy(DMD)is a rare X-linked inherited disorder caused by dystrophin deficiency,which results in sarcolemmal fragility and degeneration of skeletal and cardiac myocytes.[1]Cardiac i... To the Editor:Duchenne muscular dystrophy(DMD)is a rare X-linked inherited disorder caused by dystrophin deficiency,which results in sarcolemmal fragility and degeneration of skeletal and cardiac myocytes.[1]Cardiac involvement ismanifested asDMDcardiomyopathy,which is the leading cause of disease-related morbidity and mortality among DMD patients. 展开更多
关键词 INVOLVEMENT PATIENTS DYSTROPHY
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Effect of pEGFP-survivin on GBC-SD cell growth and chemotherapy sensitivity
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作者 Hanbin SHEN Bin ZHANG +3 位作者 Song ZHAO Qichang ZHENG Jianping GONG xiaotang cai 《Frontiers of Medicine》 SCIE CSCD 2009年第4期485-490,共6页
This paper is aimed to investigate the effect of survivin shRNA on chemotherapy resistance in human gallbladder carcinoma GBC-SD cells.The viability of human gallbladder carcinoma GBC-SD,GBC-SD/enhanced greenfluorescen... This paper is aimed to investigate the effect of survivin shRNA on chemotherapy resistance in human gallbladder carcinoma GBC-SD cells.The viability of human gallbladder carcinoma GBC-SD,GBC-SD/enhanced greenfluorescent protein(EGFP),and GBC-SD/survivin cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay,and mRNA and protein of survivin were tested by reverse transcription-polymerase chain reaction(RT-PCR)and Western blot.After the cells were treated with cisplatin(DDP)(3.0μg/mL)for the same time,cell survival rate and IC50 was detected with MTT,cell apoptosis was detected withfluorescence-activated cell sorting(FACS),and the nuclear alteration was observed by TdT-mediated deox-yuridine triphosphate nick end labeling(TUNEL).In addition,caspase-3 activity was detected by using colorimetric method.Cell viability was decreased sig-nificantly in GBC-SD/survivin cells,and survivin expres-sion was decreased significantly(mRNA and protein of survivin were decreased by 74.7%and 71.5%,respec-tively).After treatment with DDP,cell survival rate and IC50 was decreased significantly(2.03�0.24μg/mL)in GBC-SD/survivin cells,while apoptotic rate(84.3%)was elevated significantly as compared with the other two groups.There were brown apoptotic nuclei in all the cells.Caspase-3 activity in all the cells was increased atfirst and then decreased,but the caspase-3 activity in GBC-SD/survivin cells was significantly higher than the other two groups.The survivin shRNA could down-regulate the expression of survivin in GBC-SD cells significantly and improve the sensibility to chemotherapy. 展开更多
关键词 SURVIVIN SHRNA CHEMOTHERAPY SENSIBILITY GBC-SD
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