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FGW1, a protein containing DUF630 and DUF632 domains, regulates grain size and filling in Oryza sativa L. 被引量:2
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作者 Yangyang Li Peilong He +7 位作者 xiaowen wang Hongyan Chen Jile Ni Weijiang Tian Xiaobo Zhang Zhibo Cui Guanghua He Xianchun Sang 《The Crop Journal》 SCIE CSCD 2023年第5期1390-1400,共11页
Grain filling influences grain size and quality in cereal crops. The molecular mechanisms that regulate grain endosperm development remain elusive. In this study, we characterized a filling-defective and grain width m... Grain filling influences grain size and quality in cereal crops. The molecular mechanisms that regulate grain endosperm development remain elusive. In this study, we characterized a filling-defective and grain width mutant, fgw1, whose mutation increased rice seed width mainly via cell division and expansion in grains. Sucrose contents were higher but starch contents lower in the fgw1 mutant during the grainfilling stage, resulting in inferior endosperm of opaque, white appearance with loosely packed starch granules. Map-based cloning revealed that FGW1 encoded a protein containing DUF630/DUF632domains, localized in the plasma membrane with preferential expression in the panicle. RNA interference in FGW1 resulted in increased grain width and weight, whereas overexpression of FGW1 led to slightly narrower kernels and better grain filling. In a yeast two-hybrid assay, FGW1 interacted directly with the 14–3–3 protein GF14f, bimolecular fluorescence complementation verified that the site of interaction was the membrane, and the mutated FGW1 protein failed to interact with GF14f. The expression of GF14f was down-regulated in fgw1, and the activities of AGPase, StSase, and SuSase in the endosperm of fgw1increased similarly to those of a reported GF14f-RNAi. Transcriptome analysis indicated that FGW1 also regulates cellular processes and carbohydrate metabolism. Thus, FGW1 regulated grain formation via the GF14f pathway. 展开更多
关键词 Seed size Grain filling DUF630/DUF632 Starch synthesis Rice
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Risk Prediction Model for Surgical Treatment of Ruptured Corpus Luteum in the Ovary
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作者 Yuqi Qiu Sufei wang +2 位作者 Yong Chen Wenrong He xiaowen wang 《Yangtze Medicine》 2023年第2期63-75,共13页
Objective: To explore the related factors of surgical treatment of patients with corpus luteum rupture and establish a risk prediction model of surgical treatment of corpus luteum rupture. Methods: 222 patients with c... Objective: To explore the related factors of surgical treatment of patients with corpus luteum rupture and establish a risk prediction model of surgical treatment of corpus luteum rupture. Methods: 222 patients with corpus luteum rupture treated in Jingzhou First People’s Hospital from January 2015 to March 2022 were analyzed retrospectively, including 45 cases of surgery and 177 cases of conservative treatment. The training set and validation set were randomly assigned according to 7:3. We collected the basic information, laboratory and ultrasonic examination data of 222 patients. Logistic regression analysis was used to determine the independent risk factors and combined predictors of surgical treatment of corpus luteum rupture. The risk prediction model was established and the nomogram was drawn. The discrimination and calibration of the prediction model were verified and evaluated by receiver operating characteristic (ROC) curve, calibration curve and Hosmer-Lemeshow goodness of fit test;Decision curve analysis (DCA) was used to evaluate the clinical effectiveness of the prediction model. Results: Univariate logistic regression showed that whole abdominal pain (OR: 2.314, 95% CI: 1.090 - 4.912), abdominal muscle tension (OR: 2.379, 95% CI: 1.112 - 5.089), adnexal mass ≥ 4 cm (OR: 3.926, 95% CI: 1.771 - 8.266), hemoglobin Conclusion: The nomogram prediction model containing three predictive variables (hemoglobin, depth of pelvic effusion under ultrasound and cervical lifting pain) can be used to predict the risk of surgical treatment in patients with corpus luteum rupture. 展开更多
关键词 Corpus Luteum Rupture Surgical Treatment Prediction Model
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The Activity of AQP9 Is Mediated MAPK in Arsenic-Treated Mouse Model of Hepatocellular Carcinoma
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作者 Qianqian wang Ping wang +5 位作者 Xiaozhen wang xiaowen wang Zeyan Zhang Fuzhi Lian Rong Zhen Yifei Cao 《Advances in Bioscience and Biotechnology》 CAS 2022年第12期545-553,共9页
Background: Arsenic metabolism is primarily undergoing in hepatocytes, but the underlying mechanisms are to be defined. It is essential to study the response of aquaporin AQP9, protein kinase p38, and JNK with the sti... Background: Arsenic metabolism is primarily undergoing in hepatocytes, but the underlying mechanisms are to be defined. It is essential to study the response of aquaporin AQP9, protein kinase p38, and JNK with the stimulation of arsenic in mouse models of hepatocellular carcinoma. Methods: Mouse model of hepatocellular carcinoma (HCC) was induced with H22 cells injected subcutaneously on the lateral side of each right axilla in C57BL/6 mice. Then, western blotting and co-immunoprecipitation were used to detect the protein expression and phosphorylation of molecules AQP9, p38 and JNK in mouse models of hepatocellular carcinoma, respectively. The hepatocellular distribution of AQP9 was examined by the immunofluorescent method. Results: In both wild mice and a mouse model of liver tumor, there was no significant difference in the expressions of AQP9, protein kinase p38, and JNK after arsenic treatment, but the phosphorylation expression levels of the three were significantly increased to varying degrees, and the tumor model Compared with the wild-type group, the expression increased. Laser confocal experiments showed that in HepG2 cells, phosphorylated AQP9 was mainly distributed on the cell membrane under the stimulation of arsenic. Conclusion: Arsenic stimulation can increase the phosphorylation of AQP9, p38, and JNK in both wild-type C57 mice and liver tumor mice models. Arsenic stimulation facilitates phosphorylated-AQP9 predominantly distributed on the cell membrane of hepatoma cells HepG2. 展开更多
关键词 AQP9 PHOSPHORYLATION P38 JNK ARSENIC
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Assess of the Value of Semen Examination Items in the Diagnosis of Male Infertility
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作者 xiaowen wang Yanan Zhang +2 位作者 Yonghui Liu Shizong Huang Zhijun Zang 《Yangtze Medicine》 2021年第1期1-11,共11页
<strong>Background:</strong> Although semen analysis is a basic method to evaluate male fertility, there was a certain deviation between the semen examination and the actual situation in clinical applicati... <strong>Background:</strong> Although semen analysis is a basic method to evaluate male fertility, there was a certain deviation between the semen examination and the actual situation in clinical application. <strong>Objective:</strong> To evaluate the application value of semen examination items in the diagnosis of male infertility. <strong>Method:</strong> Males recruited from the Third Affiliated Hospital of Sun Yat-sen University from June 2017 to December 2018 were divided into three groups: normal fertility group, physical examination group (premarital physical examination), and male infertility group. Each group received the following examination such as semen analysis (including semen volume, sperm concentration, progressive motility, total motility, round cell concentration), acrosin activity, and sperm morphology. All the semen analysis items mentioned above were operated according to the <em>WHO laboratory manual for the examination and procession of human semen</em> (<em>Fifth edition</em>) and related documents. <strong>Results:</strong> The normal fertility group and physical examination group showed significantly higher levels in sperm concentration, progressive motility, total motility, and sperm morphology than those in the male infertility group (<em>P</em> < 0.05);both the normal fertility group and the physical examination group have a higher qualified rate in sperm concentration, progressive motility, total motility and sperm morphology than the male infertility group, all <em>P</em> < 0.001. The percentage of patients having all qualified items in each group was 28.13%, 6.67%, and 6.51% separately. <strong>Conclusion:</strong> The diagnosis efficiency was higher in sperm concentration, progressive motility, total motility, and sperm morphology than in other semen examination items. A more objective and efficient method of semen examination should be explored to facilitate the diagnoses of male infertility in the future. 展开更多
关键词 Male Infertility Semen Analysis Application Value
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Prognostic Factors in COVID-19 Pneumonia with Severe Acute Respiratory Distress Syndrome: An Observational Study
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作者 xiaowen wang Liya Zhu +7 位作者 Jian Liu Keming Chen Xiangqiong Liu Zhiqiang Liu Chenqi Xin Yujie Chen Jinzhi Lu Cunjian Yi 《Yangtze Medicine》 2021年第4期249-265,共17页
<strong>Purpose:</strong> To identify prognostic factors in patients with coronavirus disease 2019 (COVID-19)-associated severe acute respiratory distress syndrome (ARDS). <strong>Methods:</strong... <strong>Purpose:</strong> To identify prognostic factors in patients with coronavirus disease 2019 (COVID-19)-associated severe acute respiratory distress syndrome (ARDS). <strong>Methods:</strong> In this study, we included 45 patients with COVID-19-associated ARDS hospitalized at The First Affiliated Hospital of Yangtze University in Jingzhou, Hubei, China, between January 22, 2020, and March 6, 2020. Clinical data and outcomes were reviewed and analyzed according to the Berlin definition. <strong>Findings:</strong> Men were more likely to develop severe ARDS than women (11 [91.7%] vs. 1 [8.3%]). Factors associated with severe ARDS included sex (male) (hazard ratio [HR], 13.75;95% confidence interval [CI], 1.45 - 130.24), neutrophil count (HR, 55.00;95% CI, 5.02 - 602.15), lymphocyte count (HR, 40.00;95% CI, 4.83 - 331.00), prothrombin time (HR, 12.14;95% CI, 1.19 - 123.62), D-dimer (HR, 11.00;95% CI, 1.16 - 103.94), total bilirubin levels (HR, 5.00;95% CI, 0.93 - 26.79), albumin (HR, 17.5;95% CI, 2.67 - 114.85), blood urea nitrogen levels (HR, 28.60;95% CI, 2.89 - 283.06), lactate dehydrogenase levels (HR, 6.00;95% CI, 1.17 - 30.73), and C-reactive protein levels (HR, 15.87;95% CI, 2.40 - 111.11). <strong>Conclusion:</strong> Laboratory indicators, such as neutrophil count and lymphocyte count, could play an important role in the diagnosis of severe ARDS and guide clinical decision-making for patients with ARDS. 展开更多
关键词 Acute Respiratory Distress Syndrome Coronavirus Disease 2019 PNEUMONIA Prognostic Factors
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Derivation of Plastic Work Rate Done per Unit Volume for Mean Yield Criterion and Its Application 被引量:8
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作者 Dewen ZHAO Yingjie XIE +1 位作者 xiaowen wang Xianghua LIU 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2005年第4期433-436,共4页
In Haigh Westergaard stress space linear combination of twin shear stress and Tresca yield functions is called the mean yield (MY) criterion. The mathematical relationship of the criterion and its plastic work rate ... In Haigh Westergaard stress space linear combination of twin shear stress and Tresca yield functions is called the mean yield (MY) criterion. The mathematical relationship of the criterion and its plastic work rate done per unit volume were derived. A generalized worked example of slab forging was analyzed by the criterion and its corresponding plastic work rate done per unit volume. Then, the precision of the solution was compared with those by Mises and Twin shear stress yield criterions, respectively. It turned out that the calculated results by MY criterion were in good agreement with those by Mises criterion. 展开更多
关键词 Mean yield criterion Plastic work rate done per unit volume Functional linearization Slab forging
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Extraction and analysis of saline soil deformation in the Qarhan Salt Lake region (in Qinghai, China) by the sentinel SBAS-InSAR technique 被引量:4
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作者 Wei Xiang Rui Zhang +6 位作者 Guoxiang Liu xiaowen wang Wenfei Mao Bo Zhang Jialun Cai Jiawen Bao Yin Fu 《Geodesy and Geodynamics》 CSCD 2022年第2期127-137,共11页
The instability of saline soil foundation affects the safety of artificial construction,and may cause ground collapse,building destruction and road damage.It is fundamental to reduce the potential engineering geologic... The instability of saline soil foundation affects the safety of artificial construction,and may cause ground collapse,building destruction and road damage.It is fundamental to reduce the potential engineering geological disasters by exploring the dynamic evolution of saline soil.Most of the previous researches of saline soil were conducted by simulated experiments and traditional geodetic surveying methods.Restricted to the limited sampling points,the spatiotemporal evolution characteristics of saline soil were difficult to find out in a large scale.This paper exploited SBAS-InSAR method to extract the deformation of the Qarhan Salt Lake(in Qinghai,China)section along Qinghai-Tibet railway,based on 119 image data acquired by Sentinel-1A from 2015 to 2020.The results showed that the deformation trends varied frequently in this saline mud flat.Between the adjacent areas with different deformation trends along Qinghai-Tibet railway,discontinuities caused by uplift and subsidence were very obvious.Besides,among the areas with brine exploitation or artificial construction,the maximum subsidence rate reached50 mm/yr,and the maximum cumulative subsidence exceeded 320 mm in the latest 5 years.In the saline mud flat closed to the rivers and lakes,the deformation trend was continuous uplift.Nevertheless,there were obvious seasonal deformation characteristics in those areas far away from the water body.Further analysis found out a sharply subsidence caused by the salt collapsibility appeared in rainy seasons.While an uplift trend induced by the salt swelling or frost heave was remarkable in dry seasons.Subsequently,the relationships were analyzed between the time series deformation and external environmental fac-tors.Furthermore,the deformation mechanism of saline soilin the Qarhan Salt Lake region was inter-preted then.In general,this study provides complete spatiotemporal evolution information of saline soil,and demonstrates the deformation characteristics of saline soil in the Qarhan Salt Lake region suc-cessfully.Related results would contribute to the safety monitoring for large-scale infrastructure con-struction in the saline soil areas. 展开更多
关键词 QarhanSalt lake Saline soil SBAS-InSAR Deformation monitoring Qinghai-Tibet railway
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Effects of Rat Cytomegalovirus on the Nervous System of the Early Rat Embryo 被引量:4
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作者 Xiuning Sun YingJun Guan +6 位作者 Fengjie Li Xutong Li xiaowen wang Zhiyu Guan Kai Sheng Li Yu Zhijun Liu 《Virologica Sinica》 CAS CSCD 2012年第4期234-240,共7页
The purpose of the study was to investigate the impact of rat cytomegalovirus (RCMV) infection on the development of the nervous system in rat embryos, and to evaluate the involvement of Wnt signaling pathway key mo... The purpose of the study was to investigate the impact of rat cytomegalovirus (RCMV) infection on the development of the nervous system in rat embryos, and to evaluate the involvement of Wnt signaling pathway key molecules and the downstream gene neurogenin 1 (Ngnl) in RCMV infected neural stem cells (NSCs). Infection and control groups were established, each containing 20 pregnant Wistar rats. Rats in the infection group were inoculated with RCMV by intraperitoneal injection on the first day of pregnancy. Rat E20 embryos were taken to evaluate the teratogenic rate. NSCs were isolated from El3 embryos, and maintained in vitro. We found: 1) Poor fetal development was found in the infection group with low survival and high malformation rates. 2) The proliferation and differentiation of NSCs were affected. In the infection group, NSCs proliferated more slowly and had a lower neurosphere formation rate than the control. The differentiation ratio from NSCs to neurons and glial cells was significantly different from that of the control, showed by immunofluorescenee staining. 3) Ngnl mRNA expression and the nuclear p-catenin protein level were significantly lower than the control on day 2 when NSCs differentiated. 4) The Morris water maze test was performed on 4-week pups, and the infected rats were found worse in learning and memory ability. In a summary, RCMV infection caused abnormalities in the rat embryonic nervous system, significantly inhibited NSC proliferation and differentiation, and inhibited the expression of key molecules in the Wnt/β-catenin signaling pathway so as to affect NSCs differentiation. This may be an important mechanism by which RCMV causes embryonic nervous system abnormalities. 展开更多
关键词 RCMV NSCS Proliferation and differentiation WNT/Β-CATENIN Ngnl
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Monitoring subsidence rates along road network by persistent scatterer SAR interferometry with high-resolution TerraSAR-X imagery 被引量:5
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作者 Bing Yu Guoxiang Liu +5 位作者 Rui Zhang Hongguo Jia Tao Li xiaowen wang Keren Dai Deying Ma 《Journal of Modern Transportation》 2013年第4期236-246,共11页
Ground subsidence is one of the key factors damaging transportation facilities, e.g., road networks consisting of highways and railways. In this paper, we propose to apply the persistent scatterer synthetic aperture r... Ground subsidence is one of the key factors damaging transportation facilities, e.g., road networks consisting of highways and railways. In this paper, we propose to apply the persistent scatterer synthetic aperture radar interferometry (PS-InSAR) approach that uses high- resolution TerraSAR-X (TSX) imagery to extract the regional scale subsidence rates (i.e., average annual sub- sidence in mm/year) along road networks. The primary procedures involve interferometric pair selection, interfer- ogram generation, persistent scatterer (PS) detection, PS networking, phase parameterization, and subsidence rate estimation. The Xiqing District in southwest Tianjin (China) is selected as the study area. This district contains one railway line and several highway lines. A total of 15 TSX images covering this area between April 2009 and June 2010 are utilized to obtain the subsidence rates by using the PS-InSAR (PSI) approach. The subsidence rates derived from PSI range from -68.7 to -1.3 mm/year. These findings show a significantly uneven subsidence pattern along the road network. Comparison between the PSI-derived subsidence rates and the leveling data obtained along the highways shows that the mean and standard deviation (SD) of the discrepancies between the two types of subsidence rates are 0.1 and 4-3.2 mm/year, respectively. The results indicate that the high-resolution TSX PSI is capable of providing comprehensive and detailed subsidence information regarding road networks with millimeter-level accuracy. Further inspections under geo- logical conditions and land-use categories in the study area indicate that the observed subsidence is highly related to aquifer compression due to groundwater pumping. Therefore, measures should be taken to mitigate groundwater extraction for the study area. 展开更多
关键词 SUBSIDENCE Road network - Persistent scatterer interferometry TERRASAR-X HIGHWAY RAILWAY
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氮掺杂石墨烯制备及其应用研究进展 被引量:1
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作者 康婉文 全海燕 +12 位作者 黄永浩 罗品 梁耀恒 钟碧琪 李政 朱武青 莫昌泳 吴基平 廖宏杰 王晓文 陈东初 张敏 户华文 《纳米技术》 2019年第1期17-31,共15页
石墨烯作为物理、化学、生物医药、材料科学等学科领域的一大研究热点,它存在以下几个方面的局限性而在一定程度上限制了它的广泛应用:1) 理想石墨烯材料具有的零带隙电子结构限制了它在光电领域的应用,2) 具有高表面能的石墨烯片层极... 石墨烯作为物理、化学、生物医药、材料科学等学科领域的一大研究热点,它存在以下几个方面的局限性而在一定程度上限制了它的广泛应用:1) 理想石墨烯材料具有的零带隙电子结构限制了它在光电领域的应用,2) 具有高表面能的石墨烯片层极易团聚而致使其散失所特有的诸多优异性能,3) 由于与其它材料的界面结合牢度不够,表面呈现惰性的石墨烯片层不易与其它材料复合。为了使石墨烯材料获得更加广泛的应用,功能化改性处理一直都被作为一个研究热点而被广泛深入研究。功能化石墨烯不仅可以利用石墨烯诸多本征优异性能,还可以赋予其新的结构及功能,从而实现更广泛的应用。调控石墨烯物理化学性质的热门方法之一是通过利用像无机非金属原子等异质原子进行掺杂处理,使石墨烯重组子晶格结构,打破其原有的对称性和规则嵌套结构。氮原子具有相比于其它无机非金属原子更接近碳原子的大小,故与石墨烯有着更好的相容性,易于掺杂进入石墨烯的晶格中而获得稳定的氮掺杂石墨烯(NG)材料。更重要的是,氮元素的掺入将会产生N-C键,其中毗邻N原子的C原子将会带有更多的正电荷,从而能有效增强石墨烯材料的电负性,这种电子吸附性的增强可为氧化还原反应创造更佳的催化条件,这些特性使得NG的研究及应用成为了各领域的重要方向。本论文综述了近几年NG的制备方法,分析比较了各制备方法的优缺点,并对其现阶段研究瓶颈问题和在催化、超级电容器、光催化、生物传感、抗菌等应用方向进行了总结性展望。本综述论文将为开发更多高性能NG基材料用于解决各科技和工业领域基础理论和实际技术问题和挑战具有一定参考价值。 展开更多
关键词 氮掺杂石墨烯 氧化石墨烯 改性 功能化 杂元素原子
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Sperm Quality and Environment: A Retrospective, Cohort Study among 21,715 Semen Samples in a Southern Province of China
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作者 Yanan Zhang Shizong Huang xiaowen wang 《Advances in Sexual Medicine》 2021年第4期73-85,共13页
<span style="font-family:Verdana;">To explore the differences of male semen parameters in different seasons of the year, so as to explore the potential climatic factors affecting spermatogenesis and ma... <span style="font-family:Verdana;">To explore the differences of male semen parameters in different seasons of the year, so as to explore the potential climatic factors affecting spermatogenesis and male reproductive ability</span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> we retrospectively analyzed 21,715 semen analysis data from January 2018 to February 2021, grouped by year and season, and finally the relationships among semen parameters and semen and meteorological parameters were compared. Environmental exposures prior to 3 months were analyzed and correlation analysis was performed.</span><span style="font-family:Verdana;">The semen concentration decreased year by year (p < 0.01). However, the Progressive motility (PR) and total PR number had been increased (p < 0.01). There were statistical differences in sperm parameters which include semen volume, sperm concentration, total sperm number, progressive motility (PR), total PR number and total motility in different seasons, winter and spring were better than summer and autumn (p < 0.01). Total sperm number and sperm concentration were positively correlated with PR (R = 0.420, R = 0.440, p < 0.01). There was no correlation between daylight duration and semen parameters. Sperm parameters were positively or negatively correlated with environmental temperature, air pressure or humidity which had an overall effect on semen quality. It is suggested that seasonal factors should be considered when evaluating male reproductive ability. Besides referring to conventional semen parameters, other factors such as season and climate should also be considered.</span></span> 展开更多
关键词 INFERTILITY Seasonal Sperm Pattern Semen Quality Climatic Factors Correlation Analysis
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Genetic resources of lotus (Nelumbo) and their improvement
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作者 Ping Zhou Qijiang Jin +8 位作者 Ping Qian Yanjie wang xiaowen wang Huiyan Jiang Dongrui Yao Xiaojing Liu Fengjun Liu Jun Li Yingchun Xu 《Ornamental Plant Research》 2022年第1期39-54,共16页
Lotus(Nelumbo)is one of the top ten flowers in China,which has high ornamental,edible and medicinal value.Lotus has a been cultivated for thousands of years.Through discovery and cultivation,more than 4,000 cultivars ... Lotus(Nelumbo)is one of the top ten flowers in China,which has high ornamental,edible and medicinal value.Lotus has a been cultivated for thousands of years.Through discovery and cultivation,more than 4,000 cultivars have been recorded.However,the information related to lotus breeding is quite scattered,and the related genetic rules and trait formation mechanisms are still poorly understood,which has caused a greater impact on lotus genetic breeding.This article systematically introduces lotus germplasm resources,including wild species and cultivated species,summarizes lotus breeding methods and breeding directions,and focuses on the latest progress in the isolation and functional identification of structural and regulatory genes related to important horticultural traits.Prospects for the protection and utilization of lotus resources,breeding and industrialization are reported. 展开更多
关键词 LOTUS BREEDING FLOWERS
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Hippo(YAP)-autophagy axis protects against hepatic ischemia-reperfusion injury through JNK signaling 被引量:1
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作者 Shuguang Zhu xiaowen wang +7 位作者 Haoqi Chen Wenfeng Zhu Xuejiao Li Ruiwen Cui Xiaomeng Yi Xiaolong Chen Hua Li Genshu wang 《Chinese Medical Journal》 SCIE CAS CSCD 2024年第6期657-668,共12页
Background:Hepatic ischemia-reperfusion injury(HIRI)remains a common complication during liver transplantation(LT)in patients.As a key downstream effector of the Hippo pathway,Yes-associated protein(YAP)has been repor... Background:Hepatic ischemia-reperfusion injury(HIRI)remains a common complication during liver transplantation(LT)in patients.As a key downstream effector of the Hippo pathway,Yes-associated protein(YAP)has been reported to be involved in various physiological and pathological processes.However,it remains elusive whether and how YAP may control autophagy activation during ischemia-reperfusion.Methods:Human liver tissues from patients who had undergone LT were obtained to evaluate the correlation between YAP and autophagy activation.Both an in vitro hepatocyte cell line and in vivo liver-specific YAP knockdown mice were used to establish the hepatic ischemia-reperfusion models to determine the role of YAP in the activation of autophagy and the mechanism of regulation.Results:Autophagy was activated in the post-perfusion liver grafts during LT in patients,and the expression of YAP positively correlated with the autophagic level of hepatocytes.Liver-specific knockdown of YAP inhibited hepatocytes autophagy upon hypoxia-reoxygenation and HIRI(P<0.05).YAP deficiency aggravated HIRI by promoting the apoptosis of hepatocytes both in the in vitro and in vivo models(P<0.05).Attenuated HIRI by overexpression of YAP was diminished after the inhibition of autophagy with 3-methyladenine.In addition,inhibiting autophagy activation by YAP knockdown exacerbated mitochondrial damage through increasing reactive oxygen species(P<0.05).Moreover,the regulation of autophagy by YAP during HIRI was mediated by AP1(c-Jun)N-terminal kinase(JNK)signaling through binding to the transcriptional enhanced associate domain(TEAD).Conclusions:YAP protects against HIRI by inducing autophagy via JNK signaling that suppresses the apoptosis of hepatocytes.Targeting Hippo(YAP)-JNK-autophagy axis may provide a novel strategy for the prevention and treatment of HIRI. 展开更多
关键词 YAP-signaling proteins AUTOPHAGY c-Jun N-terminal kinase Ischemia-reperfusion injury Liver transplantation
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A genomic association study revealing subphenotypes of childhood steroid-sensitive nephrotic syndrome in a larger genomic sequencing cohort
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作者 Han Chan Fenfen Ni +20 位作者 Bo Zhao Huimin Jiang Juanjuan Ding Li wang xiaowen wang Jingjing Cui Shipin Feng Xiaojie Gao Xueying Yang Huan Chi Hao Lee Xuelan Chen Xiaoqin Li Jia Jiao Daoqi Wu Gaofu Zhang Mo wang Yupeng Cun Xiongzhong Ruan Haiping Yang Qiu Li 《Genes & Diseases》 SCIE CSCD 2024年第4期400-410,共11页
Dissecting the genetic components that contribute to the two main subphenotypes of steroid-sensitive nephrotic syndrome(SSNS)using genome-wide association studies(GWAS)strategy is important for understanding the disea... Dissecting the genetic components that contribute to the two main subphenotypes of steroid-sensitive nephrotic syndrome(SSNS)using genome-wide association studies(GWAS)strategy is important for understanding the disease.We conducted a multicenter cohort study(360 patients and 1835 controls)combined with a GWAS strategy to identify susceptibility var-iants associated with the following two subphenotypes of ssNS:steroid-sensitive nephrotic syn-drome without relapse(SSNswR,181 patients)and steroid-dependent/frequent relapse nephrotic syndrome(SDNS/FRNS,179 patients).The distribution of two single-nucleotide poly-morphisms(SNPs)in ANKRD36 and ALPG was significant between SSNSWR and healthy controls,and that of two SNPs in GAD1 and HLA-DQA1 was significant between SDNS/FRNS and healthy controls.Interestingly,rs1047989 in HLA-DQA1 was a candidate locus for SDNS/FRNS but not for SSNSWR.No significant SNPs were observed between SSNSWR and SDNS/FRNS.Meanwhile,chromosome 2:171713702 in GAD1 was associated with a greater steroid dose(>0.75 mg/kg/d)upon relapse to first remission in patients with SDNS/FRNS(odds ratio=3.14;95%confidence interval,0.97-9.87;P=0.034).rs117014418 in APOL4 was significantly associated with a decrease in eGFR of greater than 20%compared with the baseline in SDNS/FRNS patients(P=0.0001).Protein-protein intersection network construction suggested that HLA-DQA1 and HLA-DQB1 function together through GSDMA.Thus,SSNSWR belongs to non-HLA region-dependent nephropathy,and the HLA-DQA/DQB region is likely strongly associated with dis-ease relapse,especially in SDNS/FRNS.The study provides a novel approach for the GWAS strategy of SsNS and contributes to our understanding of the pathological mechanisms of SSNSWRandSDNS/FRNS. 展开更多
关键词 Frequentrelapse Genome-wide association study Human leukocyte antigen region Steroid-sensitive nephrotic syndrome
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Granulomatosis with polyangiitis presented as multiple cutaneous abscesses after hysterectomy
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作者 Xiaoting Song Mengrui Li +12 位作者 Yinggai Song Ping Tu Hang Li Yang wang Rong Xu Min Chen Xiaojuan Yu Suxia wang Fude Zhou Minghui Zhao Ruoyu Li Xiaoyu Jia xiaowen wang 《Chinese Medical Journal》 SCIE CAS CSCD 2024年第6期755-756,共2页
To the Editor:A 62-year-old woman presented to the dermatology department with a 5-month history of multiple painless cutaneous abscesses and ulcers[Figure 1A],occurring 1 month after a transvaginal hysterectomy due t... To the Editor:A 62-year-old woman presented to the dermatology department with a 5-month history of multiple painless cutaneous abscesses and ulcers[Figure 1A],occurring 1 month after a transvaginal hysterectomy due to uterine prolapse.Her general condition was good before and after the surgery,with no abnormal physical signs apart from metroptosis.She reported no fever,night sweat,cough,or abdominal pain,but 10 kg weight-loss.She has no history of diabetes,autoimmune diseases,or chronic infectious diseases.There was no similar illness in her family. 展开更多
关键词 HYSTERECTOMY PAIN SURGERY
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Oxygen vacancy stabilized Bi_(2)O_(2)CO_(3)nanosheet for CO_(2)electroreduction at low overpotential enables energy efficient CO-production of formate 被引量:1
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作者 Yangyang Zhang Yanxu Chen +7 位作者 Rong Liu xiaowen wang Huanhuan Liu Yin Zhu Qizhu Qian Yafei Feng Mingyu Cheng Genqiang Zhang 《InfoMat》 SCIE CSCD 2023年第3期21-32,共12页
Bismuth-based electrocatalysts are promising candidates for electrochemical CO_(2)reduction to formate attributing to the accelerated formation of*OCHO intermediate,while the high-energy consumption remains a major ch... Bismuth-based electrocatalysts are promising candidates for electrochemical CO_(2)reduction to formate attributing to the accelerated formation of*OCHO intermediate,while the high-energy consumption remains a major challenge for practicability.Herein,we present the ultrathin Bi_(2)O_(2)CO_(3)nanosheets with abundant oxygen vacancy(Vo-BOC-NS)reconstructed from S,N-co-doped bismuth oxides that can act as durable electrocatalyst for CO_(2)-to-formate conversion with faradic efficiency(FEformate)of>95%,partial current density of 286 mA cm^(-2) with energy efficiency of 73.8%at-0.62 V(vs.RHE)and low overpotential of 200 mV in a flow electrolyzer.The theoretical calculations decipher that the oxygen vacancy can optimize*OCOH adsorption/desorption for the accelerated conversion kinetics.The pair-electrosynthesis tactic of formate co-production can enable a superior FE_(formate) of>90%at wide cell voltage of 2–3.3 V and total yield rate of 3742μmol cm^(-2)h^(-1)at 3.3 V,suggesting great potential for future industrialization. 展开更多
关键词 Bi_(2)O_(2)CO_(3) CO_(2)reduction electrocatalysis oxygen vacancy pair-electrosynthesis
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Targeting cancer stem cell pathways for cancer therapy 被引量:56
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作者 Liqun Yang Pengfei Shi +8 位作者 Gaichao Zhao Jie Xu Wen Peng Jiayi Zhang Guanghui Zhang xiaowen wang Zhen Dong Fei Chen Hongjuan Cui 《Signal Transduction and Targeted Therapy》 SCIE CSCD 2020年第1期2395-2429,共35页
Since cancer stem cells(CSCs)were first identified in leukemia in 1994,they have been considered promising therapeutic targets for cancer therapy.These cells have self-renewal capacity and differentiation potential an... Since cancer stem cells(CSCs)were first identified in leukemia in 1994,they have been considered promising therapeutic targets for cancer therapy.These cells have self-renewal capacity and differentiation potential and contribute to multiple tumor malignancies,such as recurrence,metastasis,heterogeneity,multidrug resistance,and radiation resistance.The biological activities of CSCs are regulated by several pluripotent transcription factors,such as OCT4,Sox2,Nanog,KLF4,and MYC.In addition,many intracellular signaling pathways,such as Wnt,NF-κB(nuclear factor-κB),Notch,Hedgehog,JAK-STAT(Janus kinase/signal transducers and activators of transcription),PI3K/AKT/mTOR(phosphoinositide 3-kinase/AKT/mammalian target of rapamycin),TGF(transforming growth factor)/SMAD,and PPAR(peroxisome proliferator-activated receptor),as well as extracellular factors,such as vascular niches,hypoxia,tumor-associated macrophages,cancer-associated fibroblasts,cancer-associated mesenchymal stem cells,extracellular matrix,and exosomes,have been shown to be very important regulators of CSCs.Molecules,vaccines,antibodies,and CAR-T(chimeric antigen receptor T cell)cells have been developed to specifically target CSCs,and some of these factors are already undergoing clinical trials.This review summarizes the characterization and identification of CSCs,depicts major factors and pathways that regulate CSC development,and discusses potential targeted therapy for CSCs. 展开更多
关键词 CANCER STEM PATHWAYS
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Multi-omics analyses of 398 foxtail millet accessions reveal genomic regions associated with domestication,metabolite traits,and antiinflammatory effects 被引量:10
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作者 Xukai Li Jianhua Gao +20 位作者 Jingyi Song Kai Guo Siyu Hou Xingchun wang Qiang He Yanyan Zhang Yakun Zhang Yulu Yang Jiaoyan Tang Hailang wang Staffan Persson Mingquan Huang Lishuai Xu Linlin Zhong Dongqin Li Yongming Liu Hua Wu Xianmin Diao Peng Chen xiaowen wang Yuanhuai Han 《Molecular Plant》 SCIE CAS CSCD 2022年第8期1367-1383,共17页
Foxtail millet(Setaria italica),which was domesticatedfromthewild speciesgreenfoxtail(Setaria viridis),isa richsource of phytonutrientsfor humans.To evaluate how breeding changed themetabolome offoxtail millet grains,... Foxtail millet(Setaria italica),which was domesticatedfromthewild speciesgreenfoxtail(Setaria viridis),isa richsource of phytonutrientsfor humans.To evaluate how breeding changed themetabolome offoxtail millet grains,we generated and analyzed the datasets encompassing the genomes,transcriptomes,metabolomes,and anti-inflammatory indices from 398 foxtail millet accessions.We identified hundreds of common variants that influence numerous secondary metabolites.We observed tremendous differences in natural variations of the metabolites and their underlying genetic architectures between distinct sub-groups of foxtail millet.Furthermore,we found that the selection of the gene alleles associated with yellow grains led to altered profiles of metabolites such as carotenoids and endogenous phytohormones.Using CRiSPR-mediated genome editing wevalidated the function of PHYTOENE SYNTHASE1(PSY1)gene in affecting milletgrain colorand quality.Interestingly,our in vitro cell inflammation assays showed that 83 metabolites in millet grains have anti-inflammatory effects.Taken together,ourmulti-omics study illustrates how the breeding history of foxtail millet has shaped its metabolite profile.The datasets we generated in this study also provide important resources for further understanding how millet grain quality is affected by different metabolites,laying the foundations for future millet genetic research and metabolome-assisted improvement. 展开更多
关键词 foxtail millet multi-omics genetic association METABOLOME transcriptome anti-inflammatory effects
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碱性果胶酶高产菌株的构建和高密度发酵 被引量:7
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作者 望潇文 向腊 +2 位作者 徐婷 卢争辉 张桂敏 《生物工程学报》 CAS CSCD 北大核心 2017年第12期2017-2027,共11页
碱性果胶酶可用于苎麻脱胶和棉织物前处理的精练工艺,与传统的高温碱煮相比,具有保护纤维、降低能耗和化学污染的优势,因此获得高表达的碱性果胶酶基因工程菌,低成本生产碱性果胶酶对于纺织工业节能减排具有重要的意义。前期研究工作已... 碱性果胶酶可用于苎麻脱胶和棉织物前处理的精练工艺,与传统的高温碱煮相比,具有保护纤维、降低能耗和化学污染的优势,因此获得高表达的碱性果胶酶基因工程菌,低成本生产碱性果胶酶对于纺织工业节能减排具有重要的意义。前期研究工作已经将来源于枯草芽孢杆菌Bacillus subtilis 168的碱性果胶酶基因pel经过密码子优化后在毕赤酵母Pichia pastoris GS115中成功表达。本研究为了提高其表达量,首先利用启动子和信号肽都优化的载体pHBM905BDM进行表达,摇瓶酶活从68 U/mL增加到100 U/mL,qPCR检测转录水平提高了27%。再利用果胶底物平板筛选水解圈大的转化子进行摇瓶发酵获得菌株GS115-pHBM905BDM-pels4,摇瓶酶活为536 U/mL。随后构建重组质粒pPIC9K-pels,电转化菌株GS115-pHBM905BDM-pels4,利用抗生素G418平板进行筛选,在含4 mg/mL的G418抗性平板上得到菌株GS115-pHBM905BDM-pPIC9K-pels1,摇瓶酶活为770 U/mL,qPCR测定含7个拷贝目的基因。最后将该菌株在5 L的发酵罐中进行高密度发酵,果胶酶酶活提高至2 271 U/mL。该碱性果胶酶酶活已达到目前酵母表达的最高水平,说明其具有很好的应用于纺织工业的潜力。 展开更多
关键词 碱性果胶酶 毕赤酵母 G418抗性标记 组氨醇脱氢酶基因(HIS4)选择标记 高密度发酵
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Diverse Asgard archaea including the novel phylum Gerdarchaeota participate in organic matter degradation 被引量:5
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作者 Mingwei Cai Yang Liu +11 位作者 Xiuran Yin Zhichao Zhou Michael WFriedrich Tim Richter Heitmann Rolf Nimzyk Ajinkya Kulkarni xiaowen wang Wenjin Li Jie Pan Yuchun Yang Ji-Dong Gu Meng Li 《Science China(Life Sciences)》 SCIE CAS CSCD 2020年第6期886-897,共12页
Asgard is an archaeal superphylum that might hold the key to understand the origin of eukaryotes, but its diversity and ecological roles remain poorly understood. Here, we reconstructed 15 metagenomic-assembled genome... Asgard is an archaeal superphylum that might hold the key to understand the origin of eukaryotes, but its diversity and ecological roles remain poorly understood. Here, we reconstructed 15 metagenomic-assembled genomes from coastal sediments covering most known Asgard archaea and a novel group, which is proposed as a new Asgard phylum named as the "Gerdarchaeota".Genomic analyses predict that Gerdarchaeota are facultative anaerobes in utilizing both organic and inorganic carbon. Unlike their closest relatives Heimdallarchaeota, Gerdarchaeota have genes encoding for cellulase and enzymes involved in the tetrahydromethanopterin-based Wood–Ljungdahl pathway. Transcriptomics showed that most of our identified Asgard archaea are capable of degrading organic matter, including peptides, amino acids and fatty acids, occupying ecological niches in different depths of layers of the sediments. Overall, this study broadens the diversity of the mysterious Asgard archaea and provides evidence for their ecological roles in coastal sediments. 展开更多
关键词 Asgard archaea Gerdarchaeota coastal sediment METAGENOME metatranscriptome
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