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二甲双胍对炎症状态下人视网膜血管内皮细胞生物学行为的保护作用及其机制 被引量:7
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作者 韩静 闫小龙 xiaoxi qiao 《中华实验眼科杂志》 CAS CSCD 北大核心 2017年第7期581-585,共5页
背景研究表明炎性过程参与糖尿病视网膜病变(DR)的发生和发展,而炎症作用的靶细胞为视网膜血管内皮细胞(RVECs)。二甲双胍是临床上常用的降血糖药物,近年来表明其可发挥保护心血管、预防肿瘤和保护血-脑屏障等多重生物学效应,... 背景研究表明炎性过程参与糖尿病视网膜病变(DR)的发生和发展,而炎症作用的靶细胞为视网膜血管内皮细胞(RVECs)。二甲双胍是临床上常用的降血糖药物,近年来表明其可发挥保护心血管、预防肿瘤和保护血-脑屏障等多重生物学效应,但其对炎症状态诱导的人视网膜血管系统异常是否具有防护作用尚不清楚。目的观察二甲双胍对肿瘤坏死因子-α(TNF-α)刺激下人视网膜血管内皮细胞(RVECs)增生、移行以及分泌单核细胞趋化蛋白-1(MCP-1)和白细胞介素-8(IL-8)的影响,探讨二甲双胍对炎症环境中人RVECs生物学行为的保护作用。方法对原代RVECs进行培养和传代并分为正常对照组、5 mmol/L二甲双胍组、TNF-α(2.5 ng/ml)组、TNF-α+不同浓度(5、10、20、40 mmol/L)二甲双胍组,分别按照分组方法在培养液中添加相应药物处理24 h。分别于处理前及处理后24 h采用Image-Pro Plus Software 7.0软件计数各组细胞数目;采用MTS法检测各组RVECs的吸光度(A490)值以评价细胞的代谢活力;采用Transwell小室法检测各组迁移细胞数;采用ELISA法检测各组细胞上清液中MCP-1和IL-8质量浓度,并对各组间检测结果进行比较。结果正常对照组、5 mmol/L二甲双胍组、TNF-α组、TNF-α+5 mmol/L二甲双胍组、TNF-α+10 mmol/L二甲双胍组、TNF-α+20 mmol/L二甲双胍组和TNF-α+40 mmol/L二甲双胍组细胞数目总体比较差异有统计学意义(F=189.31,P〈0.01);各组细胞代谢活力(A490值)分别为0.32±0.02、0.32±0.03、0.97±0.02、0.90±0.05、0.76±0.15、0.74±0.05和0.41±0.03;各组细胞移行数目分别为(1 214±49)、(1 200±45)、(1 648±43)、(1 309±48)、(1 279±73)、(961±60)和(942±106)/视野;各组细胞上清液中MCP-1质量浓度分别为(0.385±0.050)、(0.362±0.060)、(2.285±0.200)、(1.131±0.180)、(0.622±0.120)、(0.537±0.090)和(0.492±0.130)μg/ml,IL-8质量浓度分别为(0.385±0.080)、(0.390±0.120)、(1.123±0.130)、(0.899±0.180)、(0.680±0.060)、(0.417±0.090)和(0.335±0.100)μg/ml,组间A490值、移行细胞数、MCP-1质量浓度和IL-8质量浓度的总体比较差异均有统计学意义差异(F=73.31、103.89、150.92、268.32,均P〈0.01),TNF-α组细胞数目、A490值、移行细胞数、MCP-1质量浓度和IL-8质量浓度均明显高于正常对照组,TNF-α+10 mmol/L二甲双胍组、TNF-α+20 mmol/L二甲双胍组、TNF-α+40 mmol/L二甲双胍组细胞数目、A490值、移行细胞数、MCP-1质量浓度和IL-8质量浓度均明显低于TNF-α组,差异均有统计学意义(均P〈0.05)。结论二甲双胍对TNF-α诱导的人RVECs增生、移行及分泌MCP-1、IL-8的能力均有抑制作用,从而可能对炎症环境中的RVECs发挥保护作用。 展开更多
关键词 二甲双胍/药理作用 视网膜血管内皮细胞/药效 炎症 细胞增生 细胞移行 肿瘤 坏死因子-α 单核细胞趋化蛋白-1 白细胞介素培
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Numerical research on effect of overlap ratio on thermal-stress behaviors of the high-speed laser cladding coating 被引量:3
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作者 xiaoxi qiao Tongling Xia Ping Chen 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第1期555-567,共13页
High-speed laser cladding technology, a kind of surface technology to improve the wear-resistance and corrosion-resistance of mechanical parts, has the characterizations of fast scan speed, high powder utilization rat... High-speed laser cladding technology, a kind of surface technology to improve the wear-resistance and corrosion-resistance of mechanical parts, has the characterizations of fast scan speed, high powder utilization rate, and high cladding efficiency. However, its thermal-stress evolution process is very complex, which has a great influence on the residual stress and deformation. In the paper, the numerical models for the high-speed laser cladding coatings with overlap ratios of 10%,30%, and 50% are developed to investigate the influence rules of overlap ratio on the thermal-stress evolution, as well as the residual stresses and deformations. Results show that the heat accumulation can reheat and preheat the adjacent track coating and substrate, resulting in stress release of the previous track coating and decreased longitudinal stress peak of the next track coating. With the overlap ratio increasing, the heat accumulation and the corresponding maximum residual stress position tend to locate in the center of the cladding coating, where the coating has a high crack susceptibility. For a small overlap ratio of 10%, there are abrupt stress changes from tensile stress to compressive stress at the lap joint, due to insufficient input energy in the position. Increasing the overlap ratio can alleviate the abrupt stress change and reduce the residual deformation but increase the average residual stress and enlarge the hardening depth. This study reveals the mechanism of thermal-stress evolution, and provides a theoretical basis for improving the coating quality. 展开更多
关键词 high-speed laser cladding overlap ratio thermal-stress evolution residual stress and deformation numerical simulation
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Effects of square micro-pillar array porosity on the liquid motion of near surface layer 被引量:1
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作者 xiaoxi qiao Xiangjun Zhang +2 位作者 Ping Chen Yu Tian Yonggang Meng 《Chinese Physics B》 SCIE EI CAS CSCD 2020年第2期321-326,共6页
The influence rules of square micro-pillar array porosity on the liquid motion characteristics of the near-surface layer are investigated by quartz crystal microbalance(QCM).QCM is a powerful and promising technique i... The influence rules of square micro-pillar array porosity on the liquid motion characteristics of the near-surface layer are investigated by quartz crystal microbalance(QCM).QCM is a powerful and promising technique in studying the interfacial behavior,which exhibits great advantages in investigating the effects of surface microstructure,roughness,and array.In our experiments,three different arrays with the same height of about 280 nm and center distance of 200μm,but different diameters of about 78μm,139μm,and 179μm are investigated.The results indicate that when the surface array has a large porosity,its influence on the liquid motion of the near surface layer is slight,thus resulting in a small increase of half-bandwidth variation due to the additional friction energy dissipation.When the surface array has a small porosity,the array tends to make the liquid film trapped in the array oscillating with the substrate,then there may be a layer of liquid film behaving like rigid film,and it also will make the liquid motion near the array layer more complicated.Thus for the#3 surface with a small porosity,both the absolute values of frequency shift|Δf3|and half-bandwidth variationΔΓ3 increase obviously.The experimental results show good consistence with the theoretical model of Daikhin and Urbakh.This study sheds light on understanding the influence mechanism of surface array porosity on the liquid motion of near-surface layer. 展开更多
关键词 quartz crystal MICROBALANCE SQUARE micro-pillar ARRAY WETTING state permeability
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