The Principal Component Analysis of Element Contents in Chinese Herbs of the Caine Kidney Mixture

Objective: This research compares the nature of 11 kinds of Chinese herbs in the caine kidney mixture, and provides a theoretical way to concentrated prescriptions. Methods: Based on the principal component analysis of element contents in Chinese herbs, the effects of 25 chemical elements in the 11 kinds of Chinese herbs in the caine kidney mixture have been analyzed. Results: The traditional Chinese medicines of rehmannia, astragalus, mulberry, salvia miltiorrhiza, rhubarb in the caine kidney mixture play a major role in the treatment of chronic renal failure. Conclusion: The principal component analysis is very practical in the compatibility of medicines and concentrated prescriptions.

Perfluorinated iodine alkanes promote the differentiation of mouse embryonic stem cells by regulating estrogen receptor signaling

Investigating the development toxicity of perfuorinated iodine alkanes(PFIs)is critical,given their estrogenic effects through binding with estrogen receptors(ERs).In the present study,two PFIs,including dodecafuoro-1,6-diiodohexane(PFHx DI)and tridecafuorohexyl iodide(PFHx I),with binding preference to ERαand ERβ,respectively,were selected to evaluate their effects on proliferation and differentiation of the mouse embryonic stem cells(m ESCs).The results revealed that,similar to E_(2),50μmol/L PFHx DI accelerated the cell proliferation of the m ESCs.The PFI stimulation at the exposure concentrations of 2–50μmol/L promoted the differentiation of the m ESCs as characterized by the upregulation of differentiation-related biomarkers(i.e.,Otx2 and Dnmt3β)and downregulation of pluripotency genes(i.e.,Oct4,Nanog,Sox2,Prdm14 and Rex1).Comparatively,PFHx DI exhibited higher induction effect on the differentiation of the m ESCs than did PFHx I.The tests on ER signaling indicated that both PFI compounds induced exposure concentration-dependent expressions of ER signaling-related biomarkers(i.e.,ERα,ERβand Caveolin-1)in the m ESCs,and the downstream ER responsive genes(i.e.,c-fos,c-myc and c-jun)well responded to PFHx I stimulation.The role of ER in PFI-induced effects on the m ESCs was further validated by the antagonistic experiments using an ER inhibitor(ICI).The findings demonstrated that PFIs triggered ER signaling,and perturbed the differentiation program of the m ESCs,causing the potential health risk during early stage of development.

环境内分泌干扰物对雌激素受体表达与转录激活的调控效应及分析技术

环境内分泌干扰物(Endocrine disrupting chemicals, EDCs)种类繁多,来源复杂,环境污染普遍,对野生动物与人类造成了不同程度的暴露。EDCs可通过调控机体内分泌系统,干扰心血管、生殖、神经等多个系统的正常功能,从而引起机体代谢综合征、肥胖症、神经毒性、生殖发育毒性和癌症等的发生发展。污染物调控雌激素受体(Estrogen receptors, ERs)产生内分泌干扰效应是当前EDCs研究的主要方向。本文围绕ERs的基本生理特征、ERs表达与转录激活的分析检测技术,及EDCs调控不同组织来源ERs的生物学意义进行系统综述,以期从ERs激动/拮抗效应的角度,为新型化学品的内分泌干扰效应筛选及分子机制解析提供科学思路。

Theoretical and Experimental Studies on Low-Temperature Adsorption Drying of Fresh Ginger

低温度的吸附为弄干的生物材料弄干和它的申请的优点的工作原则在这份报纸被介绍。由把新鲜生姜用作弄干的材料，它的弄干的特征上的温度和相对湿度的效果被检验。结果证明弄干的率增加，温度或减少的湿度增加。平衡的弄干的时间是几乎，在不同湿度下面的一样调节，但是低平衡潮湿内容能在低湿度下面被获得。新鲜生姜的收缩特征也被学习。它的表面外观的变化被新控告的联合设备(电荷耦合器件) 和环境扫描电子显微镜学(ESEM ) 在弄干的过程期间描绘技术。弄干动力学的一个数学模型根据实验被建立。弄干的关键词低温度的吸附 - 生姜 - 弄干特征 - 收缩特征 - 数学模型 CLC 数字

Smart nanoprobe based on two-photon sensitized terbium-carbon dots for dual-mode fluorescence thermometer and antibacterial

Accurate temperature measurement plays an important role in a variety of industrial processes and scientific researches.In our work,the dual-mode temperature response nanoprobe CDs-Tb-TMPDPA containing a two-photon ligand(4-(2,4,6-trimethoxyphenyl)-pyridine-2,6-dicarboxylic acid,TMPDPA)sensitized Tb3+as a temperature-sensitive unit and carbon dots(CDs)as photothermal reagent and a fluorescence reference unit,have been designed and synthesized.In this system,both the fluorescence intensity ratio and the fluorescence lifetime have a good response to temperature.In additio n,due to the excellent photothermal conversion capability of CDs,photothermal antibacterial ability was also tested.Based on the temperature dependence of the fluorescence and the two-photon excitation characteristics of CDs-Tb-TMPDPA,the nanoprobe can also be used in the anti-counterfeiting.Our finding opens a new prospect for the use of two-photon sensitized dual-mode fluorescence thermometers.

PIMD:An Integrative Approach for Drug Repositioning Using Multiple Characterization Fusion

The accumulation of various types of drug informatics data and computational approaches for drug repositioning can accelerate pharmaceutical research and development.However,the integration of multi-dimensional drug data for precision repositioning remains a pressing challenge.Here,we propose a systematic framework named PIMD to predict drug therapeutic properties by integrating multi-dimensional data for drug repositioning.In PIMD,drug similarity networks(DSNs)based on chemical,pharmacological,and clinical data are fused into an integrated DSN(iDSN)composed of many clusters.Rather than simple fusion,PIMD offers a systematic way to annotate clusters.Unexpected drugs within clusters and drug pairs with a high iDSN similarity score are therefore identified to predict novel therapeutic uses.PIMD provides new insights into the universality,individuality,and complementarity of different drug properties by evaluating the contribution of each property data.To test the performance of PIMD,we use chemical,pharmacological,and clinical properties to generate an iDSN.Analyses of the contributions of each drug property indicate that this iDSN was driven by all data types and performs better than other DSNs.Within the top 20 recommended drug pairs,7 drugs have been reported to be repurposed.The source code for PIMD is available at https://github.com/Sepstar/PIMD/.

Impact Increment Based Hybrid Reliability Assessment Method for Transmission Systems

This paper proposes an impact-increment-based hybrid(IIHybrid)reliability assessment approach for power transmission systems.The proposed approach integrates the advantages of the impact-increment-based state enumeration method(IISE)and impact-increment-based Monte Carlo simulation(IIMC)to improve computational efficiency and accuracy.The IISE can efficiently assess the impacts of low-order contingencies.The accuracy is,however,sacrificed as highorder contingencies are usually neglected.The IIMC is more suitable for large-scale contingency spaces compared with IISE,although the calculation process is time-consuming.In this paper,the proposed IIHybrid takes advantage of its strengths while avoiding its shortcomings.The IISE and the IIMC are applied to lower and higher contingency spaces respectively.The high-order contingencies elimination technique proposed in our previous studies is still applicable to the IIHybrid.In addition,efficiency can be controlled by modifying the preset parameters to adapt to various scenarios.Case studies are performed on the IEEE 118-bus test system and PEGASE System.The results show that the proposed approach is more efficient and practicable than traditional methods.

Influence of mixed organosilane coatings with variable RGD surface densities on the adhesion and proliferation of human osteosarcoma Saos-2 cells to magnesium alloy AZ31

In the last decade,the use of magnesium and its alloys as biodegradable implant materials has become increasingly accepted.However,surface modification of these materials to control the degradation rate in the early stages of healing and improve their biocompatibility is crucial to the successful implementation of magnesium alloy implants in medicine.Cell adhesion and proliferation at the implant surface is a vital factor for successful integration of a biomaterial within the body.Cells accomplish this task by binding to ligands such as the arginine-glycine-aspartic acid peptide sequence(RGD)commonly found on adhesive proteins present in the extracellular matrix.In this paper,we report a biomimetic surface modification strategy involving deposition of a mixed organosilane layer on Mg AZ31 followed by covalent immobilization of RGD peptides through a heterobifunctional cross-linker molecule.Our results indicate that with optimized deposition conditions uniform organosilane coatings were successfully deposited on the Mg AZ31 substrate.Furthermore,we have demonstrated that the surface density of immobilized RGD can be varied by depositing organosilane layers from solutions containing two different organosilanes in specified ratios.Increases in cell adhesion and cell proliferation were observed on the surface modified substrates.