Study on the Changes of Serum Vaspin Level and its Regulatory Mechanism in Patients with Thyroid Dysfunction

Objective:To study the changes of serum vaspin levels in hyperthyroidism and hypothyroidism,and the correlation between serum vaspin and FT3,FT4,TSH and HOMA-IR.Methods:According to the diagnostic criteria of hyperthyroidism and hypothyroidism published in the 8th edition of internal medicine,the patients were divided into hyperthyroidism group(n=47),male 14,female 33,average age(35+9)years;hypothyroidism group:23 hypothyroidism patients,7 males and 16 females,with an average age of(38+10)years.The blood pressure,height and weight of all the participants were measured by a specially assigned person,and the body mass index(BMI-weight(kg)/height(M)and ankle brachial index(ABI)were calculated.Venous blood samples were drawn from all subjects after fasting for 8 hours in the moring to determine biochemical indexes.Fasting insulin(fins)was measured by chemiluminescence method,insulin resistance index(HOMA-IR,HOMA-IR==FPGx fins/22.5)was calculated by homeostasis model assessment(HOMA-IR),and HbA1c was determined by high-pressure liquid chromatography.The levels of FT3,FT4 and TSH were detected by radioimmunoassay.Serum vaspin levels were measured by ELISA Results:The level of BMI in hypothyroidism group was significantly higher than that in hyperthyroidism group and control group(P<0.01),BMI level in hyperthyroidism group was significantly lower than that in control group(P<0.05),FT3 and FT41evels in hyperthyroidism group were significantly higher than those in hypothyroidism group and control group(P<0.01),TSH level in hypothyroidism group was significantly higher than that in control group and hyperthyroidism group(P<0.01).The level of FPG in byperthyroidism group was significantly higher than that in contro1 group(P<0.01),but there was no significant difference between bhyperthyroidism group and hypothyroidism group.and fins level in hypothyroidism group was significantly higher than that in contro1 group and hyperthyroidism group(P<0.01).The leve1 of HOMA-IR in hyperthyroidism and hypothyroidism group was significantly higher than that in control group(P<0.01).Compared with the control group and the control group,the blood 1ipid indexes(TC,LDL-Q)in the hyperthyroidism group were lower than tlose in the control group and hypothyroidism group(P<0.01),and all the blood 1ipid indexes in the hypothyroidism group were significantly different from those in the control group(P<0.01).The vaspin level of hyperthyroidism group was significantly higher than that of control group and bypothyroidism group,and the latter two groups showed that the level of vaspin in hypothyroidism group was significantly lower than that of control group(P<0.05).Correlation analysis showed that serum vaspin was positively correlated with FT3 and FT4(r=0.255,P=0.005;r=0.327,P=0.001),and negatively correlated with BMI,TC and HDL(r=-0.250,P=0.006;r=-0.244,P=0.007;r=0.258,P=0.004).).Conclusion:Serum vaspin 1eve1 is related to thyroid function.The 1evel of serum vaspin increases in hyperthyroidism and decreases in hypothyroidism.Abnormal changes of fat factor vaspin are associated with thyroid dysfunction.

Cobalt protoporphyrin-induced nano-self-assembly for CT imaging,magnetic-guidance,and antioxidative protection of stem cells in pulmonary fibrosis treatment

Mesenchymal stem cells(MSCs)transplantation is a promising approach for pulmonary fibrosis(PF),however it is impeded by several persistent challenges,including the lack of long-term tracking,low retention,and poor survival of MSCs,as well as the low labeling efficiency of nanoprobes.Herein,a cobalt protoporphyrin IX(CoPP)aggregation-induced strategy is applied to develop a multifunctional nano-self-assembly(ASCP)by combining gold nanoparticle(AuNPs),superparamagnetic iron oxide nanoparticles(SPIONs),and CoPP through a facile solvent evaporation-driven approach.Since no additional carrier materials are employed during the synthesis,high loading efficiency of active ingredients and excellent biocompatibility are achieved.Additionally,facile modification of the ASCPs with bicyclo[6.1.0]nonyne(BCN)groups(named as ASCP-BCN)enables them to effectively label MSCs through bioorthogonal chemistry.The obtained ASCP-BCN could not only help to track MSCs with AuNP-based computed tomography(CT)imaging,but also achieve an SPIONs-assisted magnetic field based improvement in the MSCs retention in lungs as well as promoted the survival of MSCs via the sustained release of CoPP.The in vivo results demonstrated that the labeled MSCs improved the lung functions and alle-viated the fibrosis symptoms in a bleomycin–induced PF mouse model.Collectively,a novel ASCP-BCN multi-functional nanoagent was developed to bioorthogonally-label MSCs with a high efficiency,presenting a promising potential in the high-efficient MSC therapy for PF.

Rotavirus VP7 epitope chimeric proteins elicit cross-immunoreactivity in guinea pigs

VP7 of group A rotavirus(RVA) contains major neutralizing epitopes. Using the antigenic protein VP6 as the vector, chimeric proteins carrying foreign epitopes have been shown to possess good immunoreactivity and immunogenicity. In the present study, using modified VP6 as the vector,three chimeric proteins carrying epitopes derived from VP7 of RVA were constructed. The results showed that the chimeric proteins reacted with anti-VP6 and with SA11 and Wa virus strains.Antibodies from guinea pigs inoculated with the chimeric proteins recognized VP6 and VP7 of RVA and protected mammalian cells from SA11 and Wa infection in vitro. The neutralizing activities of the antibodies against the chimeric proteins were significantly higher than those against the vector protein VP6 F. Thus, development of chimeric vaccines carrying VP7 epitopes using VP6 as a vector could be a promising alternative to enhance immunization against RVAs.

COL4A3 mutations cause focal segmental glomerulosclerosis

In this article,there were two annotation mistakes about the precise deletion position in COL4A3 and COL4A4 mutations in ARAS patients(summarized in Figure 2E).This was caused by a bug in the old version(v3.4)of Variant Caller for Ion Torrent.The variant in family AP5 should be described as‘chr2:227942771delG’,instead of‘chr:227942770delG’,in the COL4A4 gene.The variant identified in family AP1 should be described as‘chr2:228172490delA’,instead of‘chr:228172489delA’,in the COL4A3 gene.The corrected Figure 2E is shown as below.The results and conclusions of the article are not affected,and the authors apologize for these errors.