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Foliar applications of various nitrogen(N)forms to winter wheat affect grain protein accumulation and quality via N metabolism and remobilization 被引量:1
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作者 Xiaokang Lyu Yang Liu +3 位作者 Na Li Liban Ku Yuting Hou xiaoxia wen 《The Crop Journal》 SCIE CSCD 2022年第4期1165-1177,共13页
Foliar nitrogen(N)application is an effective strategy to improve protein content and quality in wheat kernels,but the specific effects of N forms remain unclear.In a two-year field study,foliar application of various... Foliar nitrogen(N)application is an effective strategy to improve protein content and quality in wheat kernels,but the specific effects of N forms remain unclear.In a two-year field study,foliar application of various N forms(NO_(3)^(-),urea,NH_(4)^(+))at anthesis was performed to measure their effects on wheat grain protein accumulation,quality formation,and the underlying mechanisms.Foliar application of three N forms showed varying effects in improving grain gluten proteins and quality traits.Under NH_(4)^(+) application,there was more post-anthesis N uptake for grain filling,with relatively strong increase in enzyme activities and gene expression associated with N metabolism in flag leaves at 8–20 days after anthesis(DAA),whereas its promotion of grain N metabolism became weaker after 20 DAA than those under NO_(3)^(-) and urea treatments.More N was remobilized from source organs to grain under treatment with foliar NO_(3)^(-) and urea.Genes controlling the synthesis of gluten protein and disulfide bonds were upregulated by NO_(3)^(-) and urea at 20–28 DAA,contributing to increased grain protein content and quality.Overall,foliar applications of NO_(3)^(-) and urea were more effective than those of NH_(4)^(+) in increasing grain N filling.These findings show that manipulating the source–sink relationship by reinforcing grain N metabolism and N remobilization is critical for optimizing grain protein accumulation and quality formation. 展开更多
关键词 Nitrogen remobilization SOURCE-SINK Gluten protein Grain filling Quality formation
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基于肿瘤教化血小板的液体活检研究进展
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作者 文霄瑕 杨桂姝 +7 位作者 祖瑞铃 张开炯 廖钰霖 李石 余思思 王东生 冷平(综述) 罗怀超(审校) 《中国肿瘤临床》 CAS CSCD 北大核心 2021年第19期1015-1018,共4页
随着人们生活水平提高以及生活方式的转变,癌症已经成为影响现代人寿命的主要原因之一。目前,临床医生通常根据临床表现、影像学检测和血液肿瘤标志物等对癌症进行初步诊断,确诊主要还是依赖于肿瘤组织的病理分析。但是病理组织取样属... 随着人们生活水平提高以及生活方式的转变,癌症已经成为影响现代人寿命的主要原因之一。目前,临床医生通常根据临床表现、影像学检测和血液肿瘤标志物等对癌症进行初步诊断,确诊主要还是依赖于肿瘤组织的病理分析。但是病理组织取样属于侵入性操作,所以多种基于血液成分的液体活检被广泛应用于癌症的诊断,包括循环肿瘤DNA(circulating tumor DNA,ctDNA)、循环肿瘤细胞(circulating tumor cell,CTC)、细胞外囊泡(extracellular vesicles,EVs)和肿瘤教化血小板(tumoreducated platelets,TEPs)。TEPs在肿瘤早期诊断、治疗、进展与转移等领域发挥重要作用,具有较大的发展前景。本文将综述TEPs在各种肿瘤无创诊断、监测中的研究进展。 展开更多
关键词 肿瘤教化血小板 液体活检 肿瘤诊断 血小板RNA
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Combining conservation tillage with nitrogen fertilization promotes maize straw decomposition by regulating soil microbial community and enzyme activities
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作者 Houping ZHANG Xueqin TANG +6 位作者 Quanming HOU Yuanpeng ZHU Zhangheng REN Huanxin XIE Yuncheng LIAO Weiyan WANG xiaoxia wen 《Pedosphere》 SCIE CAS CSCD 2024年第4期783-796,共14页
Straw return can effectively improve farmland soil microenvironment and fertility.However,excessive straw in the topsoil adversely affects seed germination and crop growth.At present,the characteristics and key drivin... Straw return can effectively improve farmland soil microenvironment and fertility.However,excessive straw in the topsoil adversely affects seed germination and crop growth.At present,the characteristics and key driving factors of straw decomposition in dry farmlands are unclear.Based on the interactions between tillage practices including zero tillage(ZT),chisel tillage(CT),and plow tillage(PT)and nitrogen(N)fertilization,i.e.,low N(N1,180 kg ha^(-1)),normal N(N2,240 kg ha^(-1)),and high N(N3,300 kg ha^(-1)),quantitative polymerase chain reaction technology and an enzymatic detection kit were used to investigate the effects of key straw C-degrading enzyme activities and microbial abundance in soil on maize straw decomposition during the growth period of winter wheat in the winter wheat/summer maize double cropping system in a dry farmland of the Loess Plateau,China.Between 2018 and 2020,ZT and CT significantly increased winter wheat yield(by 10.94%and 12.79%,respectively)and straw decomposition velocity(by 20%and 26.67%,respectively),compared with PT.Compared to N1 and N3,N2 significantly increased wheat yield(by 4.65%and 5.31%,respectively)and straw decomposition velocity(by 26.33%and 13.21%,respectively).The partial least squares pathway modelling showed significant positive direct effects of soil moisture,NO3-,NH4+,total N,bacteria,and cellulase,laccase,and xylanase activities on straw decomposition,while soil pH,fungi,and Actinomycetes had significant negative direct effects.Overall,conservation tillage(ZT and CT)combined with N2 was beneficial for straw decomposition in the drylands of the Loess Plateau and improved straw resource utilization and basic soil fertility.The results of the study clarified the key drivers of straw decomposition in dry farmlands and provided new ideas for developing updated soil management practices and adaptive N application strategies to promote the resource utilization of straw and achieve the goals of carbon peaking and carbon neutrality. 展开更多
关键词 hydrolytic enzyme microbial biomass oxidative enzyme straw decomposition velocity tillage practice
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糖尿病视网膜病变玻璃体切割术前康柏西普玻璃体腔注射时机对VEGF表达水平和视力恢复的影响 被引量:9
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作者 文晓霞 李红军 +3 位作者 谭德文 方腾 刘有娅 蒋小均 《中华眼视光学与视觉科学杂志》 CAS CSCD 2019年第3期181-186,共6页
目的:探讨糖尿病视网膜病变(DR)玻璃体切割术前康柏西普玻璃体腔注射时机对新生血管膜血管内皮生长因子(VEGF)表达水平及患者视力恢复的影响。方法:前瞻性临床研究。将2015年5月至2017年5月重庆市开州区人民医院眼科收治的109例DR患者... 目的:探讨糖尿病视网膜病变(DR)玻璃体切割术前康柏西普玻璃体腔注射时机对新生血管膜血管内皮生长因子(VEGF)表达水平及患者视力恢复的影响。方法:前瞻性临床研究。将2015年5月至2017年5月重庆市开州区人民医院眼科收治的109例DR患者按随机数余数法分为A组(32例)、B组(43例)、C组(34例),3组均行玻璃体切割术,A组术前不予玻璃体腔内注射康柏西普,B组术前3d术眼玻璃体腔注射康柏西普,C组术前5d术眼玻璃体腔注射康柏西普,观察3组手术情况,记录手术时间、术中出血量及医源性裂孔发生情况,所有患者术中留取视网膜下新生血管膜标本,测定VEGF表达水平;观察各组并发症发生率,比较手术前后不同时间患者最佳矫正视力(BVCA)的变化情况。数据采用单因素方差分析进行比较。结果:B、C组手术时间及术中出血量均少于A组(P<0.05),术中电凝止血率、医源性视网膜裂孔率均低于A组,术后玻璃体内出血发生率较A组低(P<0.05),术后角膜水肿、高眼压、前房炎性反应发生率均低于A组(P<0.05),但B、C组组间比较差异无统计学意义(P>0.05)。B、C组新生血管膜VEGF阳性率低于A组,但B、C组组间比较差异无统计学意义(P>0.05)。术后3、6个月,3组BCVA均上升(P<0.05),B、C组术后3、6个月BCVA高于A组(P<0.05),但组间比较差异无统计学意义(P>0.05)。结论:DR患者术前玻璃体腔内注射康柏西普均可缩短手术时间,降低术中、术后并发症发生率,降低新生血管膜VEGF表达水平,促进患者术后视力恢复,但术前3d或5d注射效果并无明显差别。 展开更多
关键词 糖尿病视网膜病变 康柏西普 玻璃体切割术 时机 血管内皮生长因子
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Evidence for glaciation predating MIS-6 in the eastern Nyainqêntanglha Range, southeastern Tibet
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作者 Shangzhe ZHOU Jinming XIE +9 位作者 Xianjiao OU Liubing XU Yong SUN Xuezhen ZENG xiaoxia wen Renrong CHEN Hong YANG Xianmei HUANG Yazhong ZHOU Jinjin SUN 《Science China Earth Sciences》 SCIE EI CSCD 2021年第4期559-570,共12页
Southeastern Tibet is one of the most glaciated regions on the Tibetan Plateau both at present and during the Quaternary. Numerical dating of glacial deposits has allowed the establishment of a provisional chronology ... Southeastern Tibet is one of the most glaciated regions on the Tibetan Plateau both at present and during the Quaternary. Numerical dating of glacial deposits has allowed the establishment of a provisional chronology of Quaternary glacial fluctuations in this region, with the oldest glaciation(Guxiang Glaciation) occurring in marine oxygen isotope stage 6(MIS-6).During our recent field investigations, a morphostratigraphically older lateral moraine than that of the Guxiang Glaciation has been first identified, which is ~500–600 m above the Guxiang Glaciation moraine and discontinuously preserved on valley shoulders in the Bodui Zangbo River valley, eastern Nyainqêntanglha Range in southeastern Tibet. Considering the moraine is best preserved at Nitong Village, here we name the glacier advance which deposited the moraine as "Nitong Glaciation". Using electron spin resonance(ESR) technique, we dated the Nitong Glaciation moraine to 506.3±60.4 ka. Taking into account the age error and climatic conditions, we consider it most likely that the Nitong Glaciation occurred during MIS-12, although it might had happened sometime earlier. 展开更多
关键词 Southeastern Tibet MIS-12 Glaciation ESR dating Quaternary Glaciation
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