An improved tum-on aptasensor for thrombin detection using split aptamer fragments and graphene oxide （GO） was reported. The thrombin-binding aptamer （Aptl5） was split into two parts for target recognition, an 8-b...An improved tum-on aptasensor for thrombin detection using split aptamer fragments and graphene oxide （GO） was reported. The thrombin-binding aptamer （Aptl5） was split into two parts for target recognition, an 8-base se- quence labeled with fluorescein （FAM-Apt-A） and a 7-base oligonucleotide sequence （Apt-B）. In the absence of target protein, the fluorescence of FAM-Apt-A/Apt-B was quenched by GO through n-n stacking between GO and single-stranded DNA. However, when thrombin was introduced into the system, a target-induced G-quadruplex forms with two split aptamer fragments and thrombin. The fluorescence recovered due to weak interaction between G-quadruplex and GO. Compared to the strategy using intact aptamer, probe concentration was lowered, and an improved sensitivity was obtained. Moreover, heating process to avoid unfavorable secondary structure was avoided due to the use of shorter split aptamer fragments.展开更多
文摘An improved tum-on aptasensor for thrombin detection using split aptamer fragments and graphene oxide （GO） was reported. The thrombin-binding aptamer （Aptl5） was split into two parts for target recognition, an 8-base se- quence labeled with fluorescein （FAM-Apt-A） and a 7-base oligonucleotide sequence （Apt-B）. In the absence of target protein, the fluorescence of FAM-Apt-A/Apt-B was quenched by GO through n-n stacking between GO and single-stranded DNA. However, when thrombin was introduced into the system, a target-induced G-quadruplex forms with two split aptamer fragments and thrombin. The fluorescence recovered due to weak interaction between G-quadruplex and GO. Compared to the strategy using intact aptamer, probe concentration was lowered, and an improved sensitivity was obtained. Moreover, heating process to avoid unfavorable secondary structure was avoided due to the use of shorter split aptamer fragments.
