Genome-Wide Identification, Evolution and Expression Analyses of GA2ox Gene Family in Brassica napus L.

Gibberellin 2-oxidases(GA2ox)are important enzymes that maintain the balance of bioactive GAs in plants.GA2ox genes have been identified and characterized in many plants,but these genes were not investigated in Brassica napus.Here,we identified 31 GA2ox genes in B.napus and 15 of these BnaGA2ox genes were distributed in the A and C subgenomes.Subcellular localization predictions suggested that all BnaGA2ox proteins were localized in the cytoplasm,and gene structure analysis showed that the BnaGA2ox genes contained 2–4 exons.Phylogenetic analysis indicated that BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups,including two C_(19)-GA2ox and two C_(20)-GA2ox clades.Group 4 is a C_(20)-GA2ox Class discovered recently.Most BnaGA2ox genes had a syntenic relationship with AtGA2ox genes.BnaGA2ox genes in the C subgenome had experienced stronger selection pressure than genes in the A subgenome.BnaGA2ox genes were highly expressed in specific tissues such as those involved in growth and development,and most of them were mainly involved in abiotic responses,regulation of phytohormones and growth and development.Our study provided a valuable evolutionary analysis of GA2ox genes in monocotyledons and dicotyledons,as well as an insight into the biological functions of GA2ox family genes in B.napus.

Ni-Co/Mg-Al catalyst derived from hydrotalcite-like compound prepared by plasma for dry reforming of methane

Ni-Co bimetallic catalysts with different Ni/Co content were derived from cold plasma jet decomposition and reduction of hydrotalcite-like compounds containing Ni,Co,Mg and Al,and their catalytic performance was investigated with dry reforming of methane.Experimental results showed that the hydrotalcite-like precursors could be completely decomposed and partly reduced by cold plasma jet,and the Nicontained catalysts exhibited much higher activity than the catalyst without Ni.Especially,the catalyst with Ni/Co ratio of 8/2 achieved not only the highest conversions of 80.3%and 69.3%for CH4 and CO2,respectively,but also the best stability in 100 h testing.The catalysts were characterized by XRD,XPS,TEM and N2 adsorption techniques,and the results showed that the better performance of the 8Ni2Co bimetallic catalyst was attributed to its higher metal dispersion,smaller metal particle size,as well as the interaction effect between Ni and Co,which were brought by the special catalyst preparation method.

Ni/MgO catalyst prepared using atmospheric high-frequency discharge plasma for CO_2 reforming of methane

A new type of Ni/MgO catalyst was prepared using atmospheric high-frequency discharge cold plasma. The influences of conventional method, plasma method, and plasma plus calcination method on the catalytic activity were studied and the CO2 reforming of methane was chosen as the probe reaction. The catalysts were characterized by X-ray diffraction （XRD）, transmission electron microscope （TEM）, X-ray photoelectron spectroscopy, and CO2 temperature-programmed surface reaction techniques. The results suggested that the nickel-based catalyst prepared by plasma plus calcination method possessed a smaller particle size and a higher dispersion of active component, better low-temperature activity and enhanced anti-coking ability. The conversion of CO2 and CH4 was 90.70% and 89.37%, respectively, and the reaction lasted for 36 h without obvious deactivation under 101.325 kPa and 750°C with CO2/CH4 = 1/1.

CBP-mediated Slug acetylation stabilizes Slug and promotes EMT and migration of breast cancer cells

Slug,a member of the Snail family of transcriptional repressors,plays a key role in cancer progression,cellular plasticity,and epithelial to mesenchymal transition(EMT).Slug is a fast-turnover protein and its stability is controlled by post-translational modifications.Here,we identified that Slug is acetylated by acetyltransferase CREB-binding protein(CBP)in breast cancer cells.CBP directly interacts with the C-terminal domain of Slug through its catalytic histone acetyltransferase(HAT)domain,leading to acetylation of Slug at lysines 166 and 211.Analysis with acetylation-specific antibodies revealed that Slug is highly acetylated in metastatic breast cancer cells.Notably,Slug acetylation,mediated by CBP at lysines 166 and 211,doubles its halflife and increases its stability.Further,acetylated Slug downregulates the expression of E-cadherin,the epithelial marker,and upregulates the expression of N-cadherin and vimentin,thereby promoting breast cancer cell migration.In conclusion,the present study demonstrates that CBP-mediated Slug acetylation increases its stability,promoting EMT and migration of breast cancer cells.

Research on the Key Technology of LTCC Pressure Sensor

这篇文章基于低温度介绍电容的压力传感器的制造技术过程共同开火陶器(LTCC ) 材料。作为牺牲的层用不同材料填满洞主要被讨论，并且二不同材料在制造被选择。充满 polyimide 的洞主要在 sintering 期间膨胀，这被发现，当充满的碳 ESL49000 材料更更好使洞扁平时。最后，在一条空气撤退隧道外面，离开的结构被设计并且测试在一布满建筑物压力环境，测量的频率线性地近似减少，压力适用，它证明不离开空气撤退隧道的设计明智。

Air temperature field distribution estimations over a Chinese mega-city using MODIS land surface temperature data： the case of Shanghai

The capability of obtaining spatially distrib- uted air temperature data from remote sensing measure- ments is an improvement for many environmental applications focused on urban heat island, carbon emis-sions, climate change, etc. This paper is based on the MODIS/Terra and Aqua data utilized to study the effect of the urban atmospheric heat island in Shanghai, China. The correlation between retrieved MODIS land surface tem- perature （LST） and air temperature measured at local weather stations was initially studied at different temporal and spatial scales. Secondly, the air temperature data with spatial resolutions of 250 m and 1 km were estimated from MODIS LST data and in-situ measured air temperature. The results showed that there is a slightly higher correlation between air temperature and MODIS LST at a 250 m resolution in spring and autumn on an annual scale than observed at a I km resolution. Although the distribution pattern of the air temperature thermal field varies in different seasons, the urban heat island （UHI） in Shanghai is characterized by a distribution pattern of multiple centers, with the central urban area as the primary center and the built-up regions in each district as the sub- centers. This study demonstrates the potential not only for estimating the distribution of the air temperature thermal field from MODIS LST with 250 m resolution in spring and autumn in Shanghai, but also for providing scientific and effective methods for monitoring and studying UHI effect in a Chinese mega-city such as Shanghai.