The present study used a microelectronic neural bridge comprised of electrode arrays for neural signal detection, functional electrical stimulation, and a microelectronic circuit including signal amplifying, processin...The present study used a microelectronic neural bridge comprised of electrode arrays for neural signal detection, functional electrical stimulation, and a microelectronic circuit including signal amplifying, processing, and functional electrical stimulation to bridge two separate nerves, and to restore the lost function of one nerve. The left leg of one spinal toad was subjected to external mechanical stimulation and functional electrical stimulation driving. The function of the left leg of one spinal toad was regenerated to the corresponding leg of another spinal toad using a microelectronic neural bridge. Oscilloscope tracings showed that the electromyographic signals from controlled spinal toads were generated by neural signals that controlled the spinal toad, and there was a delay between signals. This study demonstrates that microelectronic neural bridging can be used to restore neural function between different injured nerves.展开更多
Functional recovery is the final goal in the treatment of spinal cord injury. However, to date, few treatment strategies have demonstrated significant locomotor improvement in animal experiments. By using tail nerve e...Functional recovery is the final goal in the treatment of spinal cord injury. However, to date, few treatment strategies have demonstrated significant locomotor improvement in animal experiments. By using tail nerve electrical stimulation (TANES) as an open-field locomotor training method combined with glial scar ablation and cell transplantation, we have successfully promoted locomotor recovery in rats with chronic spinal cord contusion injury. The purpose of the present study is to further investigate the mechanism of TANES and its effect on electrophysiology. Spinal cord segment T10 of female, adult Long-Evans rats was contused using the NYU impactor device with 25 mm height setting. After injury, rats were randomly divided into three groups. Group I was used as a control without any treatment, group II and group III were subjected to basic treatment including glial scar ablation and transplantation of olfactory lamina propria 6 weeks after injury, and group III received TANES-induced open-field locomotor training weekly after basic treatment. All animals were allowed to survive 22 weeks, except some rats which were transected. Basso, Beattie, and Bresnahan (BBB) open-field locomotor rating scale, horizontal ladder rung walking test, and electrophysiological tests were used to assess the restoration of functional behavior and conduction. Results showed that TANES significantly improves locomotor recovery and spinal cord conduction, reflex, as well as significantly reduces the occurrence of autophagia. Additionally, after transection, trained rats still maintained higher BBB score than that of control rats. This may be related to the activity-dependent plasticity promoted by TANES-induced locomotor training.展开更多
Based on the four-times-daily ERA-Interim data with the resolution of 0.75°×0.75°,the structure and evolution characteristics of a transverse shear line(TSL)over the Qinghai-Tibet Plateau in April 2017 ...Based on the four-times-daily ERA-Interim data with the resolution of 0.75°×0.75°,the structure and evolution characteristics of a transverse shear line(TSL)over the Qinghai-Tibet Plateau in April 2017 were analyzed,and the influence mechanism of the frontogenesis and frontolysis caused by the upper-level jet on its evolution was also investigated.The results show that the TSL was mainly located near the axis of the positive vorticity zone,which was a low-value area of the wind speed.It was a shallow baroclinic system with weak ascending motion.In the vertical direction,the TSL extended to the lowest height at 00:00 and the highest at 18:00.In the horizontal direction,the length of the TSL in the east-west direction was relatively shorter during 00:00-06:00 and relatively longer during 12:00-18:00.Besides,the position of the TSL was slightly northward at 06:00 and slightly southward at 18:00.The moving direction of the TSL was generally consistent with that of the upper-level jet.In addition,the vertical stretching height of the TSL and the near-surface wind speed were positively correlated with the intensity of the upper-level jet.The calculation by frontogenesis function indicates that the frontogenesis(frontolysis)was conducive to the formation(weakening)and strengthening(dissipation)of the TSL.The horizontal deformation-induced and diabatic heating-induced frontogenesis were favorable for the formation of the TSL,while the middle-level horizontal convergence-induced and diabatic heating-induced frontogenesis were beneficial to its maintenance.Besides,the moving direction and baroclinicity of the TSL over the Qinghai-Tibet Plateau were determined by the horizontal deformation-induced frontogenesis.In the frontogenesis function,the terms of horizontal deformation and horizontal convergence together determined the position of the TSL,and the diabatic heating term was conducive to the upward extension of the TSL.展开更多
Glycolytic metabolism enzymes have been implicated in the immunometabolism field through changes in metabolic status. PGK1 is a catalytic enzyme in the glycolytic pathway. Here, we set up a high-throughput screen plat...Glycolytic metabolism enzymes have been implicated in the immunometabolism field through changes in metabolic status. PGK1 is a catalytic enzyme in the glycolytic pathway. Here, we set up a high-throughput screen platform to identify PGK1 inhibitors. DC-PGKI is an ATPcompetitive inhibitor of PGK1 with an affinity of Kd= 99.08 nmol/L. DC-PGKI stabilizes PGK1in vitro and in vivo, and suppresses both glycolytic activity and the kinase function of PGK1. In addition,DC-PGKI unveils that PGK1 regulates production of IL-1β and IL-6 in LPS-stimulated macrophages.Mechanistically, inhibition of PGK1 with DC-PGKI results in NRF2(nuclear factor-erythroid factor 2-related factor 2, NFE2L2) accumulation, then NRF2 translocates to the nucleus and binds to the proximity region of Il-1β and Il-6 genes, and inhibits LPS-induced expression of these genes. DC-PGKI ameliorates colitis in the dextran sulfate sodium(DSS)-induced colitis mouse model. These data support PGK1 as a regulator of macrophages and suggest potential utility of PGK1 inhibitors in the treatment of inflammatory bowel disease.展开更多
In animal experiments,ischemic stroke is usually induced through middle cerebral artery occlusion(MCAO),and quality assessment of this procedure is crucial.However,an accurate assessment method based on 18F-fluorodeox...In animal experiments,ischemic stroke is usually induced through middle cerebral artery occlusion(MCAO),and quality assessment of this procedure is crucial.However,an accurate assessment method based on 18F-fluorodeoxyglucose(FDG)positron emission tomography(PET)is still lacking.The difficulty lies in the inconsistent preprocessing pipeline,biased intensity normalization,or unclear spatiotemporal uptake of FDG.Here,we propose an image feature-based protocol to assess the quality of the procedure using a 3D scale-invariant feature transform and support vector machine.This feature-based protocol provides a convenient,accurate,and reliable tool to assess the quality of the MCAO procedure in FDG PET studies.Compared with existing approaches,the proposed protocol is fully quantitative,objective,automatic,and bypasses the intensity normalization step.An online interface was constructed to check images and obtain assessment results.展开更多
基金supported by the National Natural Science Foundation of China,No,90707005,61001046 and 61204018the Natural Science Foundation of Education Department of Jiangsu Province,No.11KJB510023the Special Foundation and Open Foundation of State Key Laboratory of Bioelectronics of Southeast University,No.2011E05
文摘The present study used a microelectronic neural bridge comprised of electrode arrays for neural signal detection, functional electrical stimulation, and a microelectronic circuit including signal amplifying, processing, and functional electrical stimulation to bridge two separate nerves, and to restore the lost function of one nerve. The left leg of one spinal toad was subjected to external mechanical stimulation and functional electrical stimulation driving. The function of the left leg of one spinal toad was regenerated to the corresponding leg of another spinal toad using a microelectronic neural bridge. Oscilloscope tracings showed that the electromyographic signals from controlled spinal toads were generated by neural signals that controlled the spinal toad, and there was a delay between signals. This study demonstrates that microelectronic neural bridging can be used to restore neural function between different injured nerves.
文摘Functional recovery is the final goal in the treatment of spinal cord injury. However, to date, few treatment strategies have demonstrated significant locomotor improvement in animal experiments. By using tail nerve electrical stimulation (TANES) as an open-field locomotor training method combined with glial scar ablation and cell transplantation, we have successfully promoted locomotor recovery in rats with chronic spinal cord contusion injury. The purpose of the present study is to further investigate the mechanism of TANES and its effect on electrophysiology. Spinal cord segment T10 of female, adult Long-Evans rats was contused using the NYU impactor device with 25 mm height setting. After injury, rats were randomly divided into three groups. Group I was used as a control without any treatment, group II and group III were subjected to basic treatment including glial scar ablation and transplantation of olfactory lamina propria 6 weeks after injury, and group III received TANES-induced open-field locomotor training weekly after basic treatment. All animals were allowed to survive 22 weeks, except some rats which were transected. Basso, Beattie, and Bresnahan (BBB) open-field locomotor rating scale, horizontal ladder rung walking test, and electrophysiological tests were used to assess the restoration of functional behavior and conduction. Results showed that TANES significantly improves locomotor recovery and spinal cord conduction, reflex, as well as significantly reduces the occurrence of autophagia. Additionally, after transection, trained rats still maintained higher BBB score than that of control rats. This may be related to the activity-dependent plasticity promoted by TANES-induced locomotor training.
基金Supported by Project of Qinghai Science and Technology Department (2020-ZJ-739)Project of Key Laboratory for Disaster Prevention and Mitigation of Qinghai Province (QFZ-2021-Z04)Key Project of Qinghai Provincial Meteorological Bureau (QXZ2020-03)
文摘Based on the four-times-daily ERA-Interim data with the resolution of 0.75°×0.75°,the structure and evolution characteristics of a transverse shear line(TSL)over the Qinghai-Tibet Plateau in April 2017 were analyzed,and the influence mechanism of the frontogenesis and frontolysis caused by the upper-level jet on its evolution was also investigated.The results show that the TSL was mainly located near the axis of the positive vorticity zone,which was a low-value area of the wind speed.It was a shallow baroclinic system with weak ascending motion.In the vertical direction,the TSL extended to the lowest height at 00:00 and the highest at 18:00.In the horizontal direction,the length of the TSL in the east-west direction was relatively shorter during 00:00-06:00 and relatively longer during 12:00-18:00.Besides,the position of the TSL was slightly northward at 06:00 and slightly southward at 18:00.The moving direction of the TSL was generally consistent with that of the upper-level jet.In addition,the vertical stretching height of the TSL and the near-surface wind speed were positively correlated with the intensity of the upper-level jet.The calculation by frontogenesis function indicates that the frontogenesis(frontolysis)was conducive to the formation(weakening)and strengthening(dissipation)of the TSL.The horizontal deformation-induced and diabatic heating-induced frontogenesis were favorable for the formation of the TSL,while the middle-level horizontal convergence-induced and diabatic heating-induced frontogenesis were beneficial to its maintenance.Besides,the moving direction and baroclinicity of the TSL over the Qinghai-Tibet Plateau were determined by the horizontal deformation-induced frontogenesis.In the frontogenesis function,the terms of horizontal deformation and horizontal convergence together determined the position of the TSL,and the diabatic heating term was conducive to the upward extension of the TSL.
基金the National Key Research and Development Program of China (2021ZD0203900 to Cheng Luo)the National Natural Science Foundation of China (91853205, 81821005 to Cheng Luo)+1 种基金the Science and Technology Commission of Shanghai Municipality (19XD1404700 to Cheng Luo, China)the project of National Multidisciplinary Innovation Team of Traditional Chinese Medicine supported by National Administration of Traditional Chinese Medicine to Cheng Luo, the Lingang Laboratory, Grant No. LG-QS-202206-01.
文摘Glycolytic metabolism enzymes have been implicated in the immunometabolism field through changes in metabolic status. PGK1 is a catalytic enzyme in the glycolytic pathway. Here, we set up a high-throughput screen platform to identify PGK1 inhibitors. DC-PGKI is an ATPcompetitive inhibitor of PGK1 with an affinity of Kd= 99.08 nmol/L. DC-PGKI stabilizes PGK1in vitro and in vivo, and suppresses both glycolytic activity and the kinase function of PGK1. In addition,DC-PGKI unveils that PGK1 regulates production of IL-1β and IL-6 in LPS-stimulated macrophages.Mechanistically, inhibition of PGK1 with DC-PGKI results in NRF2(nuclear factor-erythroid factor 2-related factor 2, NFE2L2) accumulation, then NRF2 translocates to the nucleus and binds to the proximity region of Il-1β and Il-6 genes, and inhibits LPS-induced expression of these genes. DC-PGKI ameliorates colitis in the dextran sulfate sodium(DSS)-induced colitis mouse model. These data support PGK1 as a regulator of macrophages and suggest potential utility of PGK1 inhibitors in the treatment of inflammatory bowel disease.
基金supported by R4012-18,C6021-19EF and GRF 16306919 from the Research Grant Council(RGC)ITS/480/18FP and MHP/033/20+2 种基金from the Innovation and Technology Commission(ITC)of the Hong Kong S.A.R.,Hetao Shenzhen-Hong Kong Science and Technology Innovation Cooperation Zone Projec(HZQB-KCZYB-2020083)the National Natural Science Foundation of China(U1809204)the Talent Program of Zhejiang Province(2021R51004)。
文摘In animal experiments,ischemic stroke is usually induced through middle cerebral artery occlusion(MCAO),and quality assessment of this procedure is crucial.However,an accurate assessment method based on 18F-fluorodeoxyglucose(FDG)positron emission tomography(PET)is still lacking.The difficulty lies in the inconsistent preprocessing pipeline,biased intensity normalization,or unclear spatiotemporal uptake of FDG.Here,we propose an image feature-based protocol to assess the quality of the procedure using a 3D scale-invariant feature transform and support vector machine.This feature-based protocol provides a convenient,accurate,and reliable tool to assess the quality of the MCAO procedure in FDG PET studies.Compared with existing approaches,the proposed protocol is fully quantitative,objective,automatic,and bypasses the intensity normalization step.An online interface was constructed to check images and obtain assessment results.
