This study evaluated the effects of feeding a microbial-derived antioxidant diet(MA)for 56 days on the growth performance of Eriocheir sinensis,as well as on digestive and metabolic enzyme activity and antioxidant cap...This study evaluated the effects of feeding a microbial-derived antioxidant diet(MA)for 56 days on the growth performance of Eriocheir sinensis,as well as on digestive and metabolic enzyme activity and antioxidant capacity under ammonia stress(400 mg/L ammonia nitrogen for 48 h).The weight gain at 28d and the specific growth rate at 56ds for crabs in the MA group were significantly higher than those in the control diet(C)group(p<0.05).Compared with the C group,the color of the hepatopancreas in the MA group was significantly improved(p<0.05).A significant decrease in the level of Hemolymph ammonia was found in the microbial antioxidant ammonia-N(MA_A)group(p<0.05),which may be related to the decrease of transaminase(AST,ALT,GDH)activities caused by MA diet(p<0.05).There is a significant increase in the digestive enzyme activities(Trypsin and Lipase),which reduced the accumulation of triglycerides in the hepatopancreas in the(MA_A)group(p<0.05).In addition,the antioxidant damage of crabs in hepatopancreas caused by ammonia nitrogen toxicity could also significantly be improved by MA fed in the(MA_A)group(p<0.05).microbe-derived antioxidants(supplemented with 0.2% in feeds)can protect the health of hepatopancreas,promote growth,and improve the ability to resist ammonia nitrogen stress for E.sinensis.展开更多
基金the support of the National Key R&D Program of China(2019YFD0900105)the capacity-promoting Project of Shanghai Engineering and Technology Center from Shanghai Municipal Science and Technology Commission(No.19DZ2284300)+5 种基金the National Natural Science Foundation of China(grant number No 41876190)the Fishery Science and Technology Projects in Jiangsu Province(grant number d2018_4)the Shandong Joint Fund(grant number U1706209)the Aquaculture Engineering Research Platform in Shanghai,established by the Shanghai Science and Technology Commission(grant number 16DZ2281200)supported by China Agriculture Research System of MOF and MARA,the program for Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-culture of Aquaculture animals(No.2021-KJ-02-12)industry-leading talent project of Yellow River Delta.(DYRC20190210).
文摘This study evaluated the effects of feeding a microbial-derived antioxidant diet(MA)for 56 days on the growth performance of Eriocheir sinensis,as well as on digestive and metabolic enzyme activity and antioxidant capacity under ammonia stress(400 mg/L ammonia nitrogen for 48 h).The weight gain at 28d and the specific growth rate at 56ds for crabs in the MA group were significantly higher than those in the control diet(C)group(p<0.05).Compared with the C group,the color of the hepatopancreas in the MA group was significantly improved(p<0.05).A significant decrease in the level of Hemolymph ammonia was found in the microbial antioxidant ammonia-N(MA_A)group(p<0.05),which may be related to the decrease of transaminase(AST,ALT,GDH)activities caused by MA diet(p<0.05).There is a significant increase in the digestive enzyme activities(Trypsin and Lipase),which reduced the accumulation of triglycerides in the hepatopancreas in the(MA_A)group(p<0.05).In addition,the antioxidant damage of crabs in hepatopancreas caused by ammonia nitrogen toxicity could also significantly be improved by MA fed in the(MA_A)group(p<0.05).microbe-derived antioxidants(supplemented with 0.2% in feeds)can protect the health of hepatopancreas,promote growth,and improve the ability to resist ammonia nitrogen stress for E.sinensis.
