Vitamin D deficiency is a common health issue around the world. We therefore evaluated the associations of semen quality with both serum and seminal plasma vitamin D levels and studied the mechanisms underlying these ...Vitamin D deficiency is a common health issue around the world. We therefore evaluated the associations of semen quality with both serum and seminal plasma vitamin D levels and studied the mechanisms underlying these by incubating spermatozoa with 1,25(OH)2D in vitro. Two hun dred and twenty-two men were in eluded in our study. Vitamin D was detected using an electrochemilumi nesce nee method. Spermatozoa used for in vitro experiments were isolated by density gradient centrifugation. Positive relationships of serum 25(OH)D with semen volume and seminal plasma fructose were identified. Seminal plasma 25(OH)D level showed no relationship with serum 25(OH)D level, while it was inversely associated with sperm concentration and positively correlated with semen volume and sperm kinetic values. In vitro, sperm kinetic parameters in creased after in cubation with 1,25(OH)2D, especially upon in cubation for 30 min with it at a concen tration of 0.1 nmol l-1. Under these in cubation conditions, the upward migratio n of spermatozoa in creased remarkably with increasing ade nosine triphosphate (ATP) con centratio n. The concentrati on of cyclic ade nosine mono phosphate (cAMP) and the activity of protei n kinase A (PKA) were both elevated, and the PKA inhibitor, N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89) reversed the in crease of ATP producti on. The conce ntrations of cytoplasmic calcium ions and n icotinamide adenine dinucleotide (NADH) were both enhanced, while mitochondrial calcium uniporter (MCU) inhibitor, Ruthenium 360 (Ru360) did not reverse the increase of ATP production. Therefore, seminal plasma vitamin D may be invoIved in regulating sperm motility, and 1,25(OH)2D may enhance sperm motility by promoting the synthesis of ATP both through the cAMP/PKA pathway and the in crease in in tracellular calcium ions.展开更多
Multiple responsive photonic prints that can be revealed by a thermal or light stimulus are prepared by locally producing interpenetrating polymer network(IPN)within photonic hydrogels.Thermo-responsive poly-(N-isopro...Multiple responsive photonic prints that can be revealed by a thermal or light stimulus are prepared by locally producing interpenetrating polymer network(IPN)within photonic hydrogels.Thermo-responsive poly-(N-isopropylacrylamide)(PNIPAM)photonic hydrogels with self-assembled monodisperse carbon-encapsulated Fe_(3)O_(4) nanoparticles(Fe_(3)O_(4)@C NPs)are first prepared by a photopolymerization process.Then photopolymerization of acrylamide(AM)is carried out within the photonic hydrogel with the help of a mask to generate an IPN structure locally.Because of the regionally different network structure and the resulting varied responsiveness,responsive photonic prints are obtained.Due to the thermo-responsive capability of the PNIPAM and the photothermal conversion capability of the incorporated Fe_(3)O_(4)@C NPs,the as-prepared photonic prints with a localized IPN structure can shift the structural color in a broad spectrum range under thermal or light stimuli.The current multiple responsive photonic prints with a localized IPN structure can realize full-color pattern display under thermal and light stimulations,providing a new path for anti-counterfeiting,display,and information encoding.展开更多
基金the Research Funds for Jiangsu Province Key Research and Development Plan (Grant No. BE2016750)Research Funds for Military Family Planning (Grant No. 16JS012)+5 种基金Six Talent Peaks Project in Jiangsu Province (Grant No. 2017-WSW-033)Chinese Medical Association Special Fund for Clinical Medical Research (Grant No. 17020350704)Foundation for Key Medical Talents in Jiangsu Province (Grant No. ZDRCA2016096)Natural Science Foundation of Jiangsu Province (Grant No. BK20170620)China Postdoctoral Science Foundation (Grant No. 2017M613434)National Natural Science Foundation of China (Grant No. 81701431 and 81701440).
文摘Vitamin D deficiency is a common health issue around the world. We therefore evaluated the associations of semen quality with both serum and seminal plasma vitamin D levels and studied the mechanisms underlying these by incubating spermatozoa with 1,25(OH)2D in vitro. Two hun dred and twenty-two men were in eluded in our study. Vitamin D was detected using an electrochemilumi nesce nee method. Spermatozoa used for in vitro experiments were isolated by density gradient centrifugation. Positive relationships of serum 25(OH)D with semen volume and seminal plasma fructose were identified. Seminal plasma 25(OH)D level showed no relationship with serum 25(OH)D level, while it was inversely associated with sperm concentration and positively correlated with semen volume and sperm kinetic values. In vitro, sperm kinetic parameters in creased after in cubation with 1,25(OH)2D, especially upon in cubation for 30 min with it at a concen tration of 0.1 nmol l-1. Under these in cubation conditions, the upward migratio n of spermatozoa in creased remarkably with increasing ade nosine triphosphate (ATP) con centratio n. The concentrati on of cyclic ade nosine mono phosphate (cAMP) and the activity of protei n kinase A (PKA) were both elevated, and the PKA inhibitor, N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89) reversed the in crease of ATP producti on. The conce ntrations of cytoplasmic calcium ions and n icotinamide adenine dinucleotide (NADH) were both enhanced, while mitochondrial calcium uniporter (MCU) inhibitor, Ruthenium 360 (Ru360) did not reverse the increase of ATP production. Therefore, seminal plasma vitamin D may be invoIved in regulating sperm motility, and 1,25(OH)2D may enhance sperm motility by promoting the synthesis of ATP both through the cAMP/PKA pathway and the in crease in in tracellular calcium ions.
基金This work was supported by the National Natural Science Foundation of China(No.52022032)the Natural Science Foundation of Hubei Province,China(No.2022CFA048)the Key Research Project of Henan Higher Education Institute,China(No.21A150066)。
文摘Multiple responsive photonic prints that can be revealed by a thermal or light stimulus are prepared by locally producing interpenetrating polymer network(IPN)within photonic hydrogels.Thermo-responsive poly-(N-isopropylacrylamide)(PNIPAM)photonic hydrogels with self-assembled monodisperse carbon-encapsulated Fe_(3)O_(4) nanoparticles(Fe_(3)O_(4)@C NPs)are first prepared by a photopolymerization process.Then photopolymerization of acrylamide(AM)is carried out within the photonic hydrogel with the help of a mask to generate an IPN structure locally.Because of the regionally different network structure and the resulting varied responsiveness,responsive photonic prints are obtained.Due to the thermo-responsive capability of the PNIPAM and the photothermal conversion capability of the incorporated Fe_(3)O_(4)@C NPs,the as-prepared photonic prints with a localized IPN structure can shift the structural color in a broad spectrum range under thermal or light stimuli.The current multiple responsive photonic prints with a localized IPN structure can realize full-color pattern display under thermal and light stimulations,providing a new path for anti-counterfeiting,display,and information encoding.
