In the paper,we solve the problems of air quality prediction and evaluation. Firstly,the original data of air quality monitoring are classified by fuzzy C means clustering algorithm（ FCM）; then the BP neural network...In the paper,we solve the problems of air quality prediction and evaluation. Firstly,the original data of air quality monitoring are classified by fuzzy C means clustering algorithm（ FCM）; then the BP neural network model to predict the level of air quality is built through the simulation training of data. Experiments show that the model has good generalization ability and strong stability,and the prediction accuracy is higher,which has certain application value.展开更多
Mammalian Sirtuin 6(SIRT6) plays an important role in the transcriptional silencing of silent mating-type loci,telomeres,and rDNA.However,the roles of porcine SIRT6 in cell proliferation are poorly understood,and a be...Mammalian Sirtuin 6(SIRT6) plays an important role in the transcriptional silencing of silent mating-type loci,telomeres,and rDNA.However,the roles of porcine SIRT6 in cell proliferation are poorly understood,and a better knowledge of these will help improve our understanding of the biological mechanisms of cell growth and development.In this study,a novel variant of porcine SIRT6(SIRT6 V2) identified by reverse transcription-polymerase chain reaction and BLAST analysis showed a 124-bp deletion compared to wild-type SIRT6 mRNA(SIRT6 V1).Two recombinant plasmids overexpressing SIRT6 V1 and SIRT6 V2 were produced and their roles in the proliferation of porcine fetal fibroblasts(PFFs) were compared.Cells transfected with SIRT6 V1 proliferated significantly faster than those transfected with SIRT6 V2(P<0.01),though both proliferated significantly faster than cells expressing an empty vector(P<0.01).These results might be caused by altered proportions of α helices and β sheets in the SIRT6 V2 structure relative to SIRT6 V1.These results indicate that overexpression of SIRT6 V1/V2 was involved in promoting PFF proliferation.Deletion of a 124-bp sequence attenuated the effects of SIRT6 on cell proliferation,possibly as a result of changes in the proportions of α helices and β sheets in the protein secondary structure.展开更多
基金Supported by Youth Fund Research Project of Science and Technology of Hebei Colleges and Universities(QN2016243)
文摘In the paper,we solve the problems of air quality prediction and evaluation. Firstly,the original data of air quality monitoring are classified by fuzzy C means clustering algorithm（ FCM）; then the BP neural network model to predict the level of air quality is built through the simulation training of data. Experiments show that the model has good generalization ability and strong stability,and the prediction accuracy is higher,which has certain application value.
基金supported by the National Special Fundation for Transgenic Species of China (2011ZX08006-004)the Earmarked Fund for Modern Agro-industry Technology Research System (CARS-36)
文摘Mammalian Sirtuin 6(SIRT6) plays an important role in the transcriptional silencing of silent mating-type loci,telomeres,and rDNA.However,the roles of porcine SIRT6 in cell proliferation are poorly understood,and a better knowledge of these will help improve our understanding of the biological mechanisms of cell growth and development.In this study,a novel variant of porcine SIRT6(SIRT6 V2) identified by reverse transcription-polymerase chain reaction and BLAST analysis showed a 124-bp deletion compared to wild-type SIRT6 mRNA(SIRT6 V1).Two recombinant plasmids overexpressing SIRT6 V1 and SIRT6 V2 were produced and their roles in the proliferation of porcine fetal fibroblasts(PFFs) were compared.Cells transfected with SIRT6 V1 proliferated significantly faster than those transfected with SIRT6 V2(P<0.01),though both proliferated significantly faster than cells expressing an empty vector(P<0.01).These results might be caused by altered proportions of α helices and β sheets in the SIRT6 V2 structure relative to SIRT6 V1.These results indicate that overexpression of SIRT6 V1/V2 was involved in promoting PFF proliferation.Deletion of a 124-bp sequence attenuated the effects of SIRT6 on cell proliferation,possibly as a result of changes in the proportions of α helices and β sheets in the protein secondary structure.
