Root production, mortality and turnover in soil profiles as affected by clipping in a temperate grassland on the Loess Plateau

Aims Clipping or mowing for hay,as a prevalent land-use practice,is considered to be an important component of global change.Root production and turnover in response to clipping have great implications for the plant survival strategy and grassland ecosystem carbon processes.However,our knowledge about the clipping effect on root dynamics is mainly based on root living biomass,and limited by the lack of spatial and temporal observations.The study aim was to investigate the effect of clipping on seasonal variations in root length production and mortality and their distribution patterns in different soil layers in semiarid grassland on the Loess Plateau.Methods Clipping was performed once a year in June to mimic the local spring livestock grazing beginning from 2014.The minirhizotron technique was used to monitor the root production,mortality and turnover rate at various soil depths(0–10,10–20,20–30 and 30–50 cm)in 2014(from 30 May to 29 October)and 2015(from 22 April to 25 October).Soil temperature and moisture in different soil layers were also measured during the study period.Important Findings Our results showed that:(i)Clipping significantly decreased the cumulative root production(P<0.05)and increased the cumulative root mortality and turnover rates of the 0–50 cm soil profile for both years.(ii)Clipping induced an immediate and sharp decrease in root length production and an increase in root length mortality in all soil layers.However,with plant regrowth,root production increased and root mortality decreased gradually,with the root production at a depth of 30–50 cm even exceeding the control in September–October 2014 and April–May 2015.(iii)Clipping mainly reduced root length production and increased root length mortality in the upper 0–20 cm soil profile with rapid root turnover.However,roots at deeper soil layers were either little influenced by clipping or exhibited an opposite trend with slower turnover rate compared with the upper soil profile,leading to the downward transport of root production and living root biomass.These findings indicate that roots in deeper soil layers tend to favour higher root biomass and longer fine root life spans to maximize the water absorption efficiency under environmental stress,and also suggest that short-term clipping would reduce the amount of carbon through fine root litter into the soil,especially in the shallow soil profile.

Nutrient uptake,physiological responses and growth of tobacco(Nicotiana tabacum L.)in soil under composite salt stress

High soil salinity imposes osmotic stress and ion toxicity in plants,leading to substantial crop yield loss worldwide.Understanding of the quantitative and dynamic physiological responses to composite soil salt stress is limited and needs to be expanded.In this study,physiological,nutritional,and biomass yield parameters of tobacco(Nicotiana tabacum L.)grown in soil with five levels of composite soil salinity(CSS),basal CSS level(control,CK)and 3(T_(1)),6(T_(2)),9(T_(3)),and 12(T_(4))times the basal CSS level,under greenhouse were determined at days 30,60,and 90 after transplanting.Leaf dry biomass significantly(P<0.05)increased at the low salinity levels applied(T_(1) and T_(2))at all three time points,whereas it progressively declined as the CSS level further increased.The leaf physiological and photosynthetic responses were more adversely affected by CSS at the early growth stage(day 30).A path coefficient analysis demonstrated that leaf proline content had the largest direct effect(-0.66),and leaf Cu content had the most significant indirect effect(0.49)on leaf dry biomass of plants.The results suggest that lower CSS levels(T_(1) and T_(2))could stimulate tobacco growth(leaf biomass yield,in particular),and higher leaf proline and Cu levels at the early growth stage may potentially increase the ability of tobacco plants to withstand the adverse effects of salinity,which could be considered for future research and development of salinity management strategies.