Anthropogenic activity-induced sinkholes pose a serious threat to building safety and human life nowadays.Real-time detection and early warning of sinkhole formation are a key and urgent problem in urban areas.This pa...Anthropogenic activity-induced sinkholes pose a serious threat to building safety and human life nowadays.Real-time detection and early warning of sinkhole formation are a key and urgent problem in urban areas.This paper presents an experimental study to evaluate the feasibility of fiber optic strain sensing nerves in sinkhole monitoring.Combining the artificial neural network(ANN)and particle image velocimetry(PIV)techniques,a series of model tests have been performed to explore the relationship between strain measurements and sinkhole development and to establish a conversion model from strain data to ground settlements.It is demonstrated that the failure mechanism of the soil above the sinkhole developed from a triangle failure plane to a vertical failure plane with increasing collapse volume.Meanwhile,the soil-embedded fiber optic strain sensing nerves allowed deformation monitoring of the ground soil in real time.Furthermore,the characteristics of the measured strain profiles indicate the locations of sinkholes and the associated shear bands.Based on the strain data,the ANN model predicts the ground settlement well.Additionally,micro-anchored fiber optic cables have been proven to increase the soil-to-fiber strain transfer efficiency for large deformation monitoring of ground collapse.展开更多
Three-dimensional (3D) printing technology is driving forward the progresses of various engineering fields, including tissue engineering. However, the pristine 3D-printed scaffolds usually lack robust functions in sti...Three-dimensional (3D) printing technology is driving forward the progresses of various engineering fields, including tissue engineering. However, the pristine 3D-printed scaffolds usually lack robust functions in stimulating desired activity for varied regeneration applications. In this study, we combined the two-dimensional (2D) hetero-nanostructures and immuno-regulative interleukin-4 (IL-4) cytokines for the functionalization of 3D-printed scaffolds to achieve a pro-healing immuno-microenvironment for optimized bone injury repair. The 2D hetero-nanostructure consists of graphene oxide (GO) layers, for improved cell adhesion, and black phosphorous (BP) nanosheets, for the continuous release of phosphate ions to stimulate cell growth and osteogenesis. In addition, the 2D hetero-nanolayers facilitated the adsorption of large content of immuno-regulative IL-4 cytokines, which modulated the polarization of macrophages into M2 phenotype. After in vivo implantation in rat, the immuno-functioned 3D-scaffolds achieved in vivo osteo-immunomodulation by building a pro-healing immunological microenvironment for better angiogenesis and osteogenesis in the defect area and thus facilitated bone regeneration. These results demonstrated that the immuno-functionalization of 3D-scaffolds with 2D hetero-nanostructures with secondary loading of immuno-regulative cytokines is an encouraging strategy for improving bone regeneration.展开更多
基金support provided by the National Natural Science Foundation of China(Grant Nos.42225702,and 42077232)the Open Research Project Program of the State Key Laboratory of Internet of Things for Smart City(University of Macao)(Grant No.SKL-IoTSC(UM)-2021-2023/ORP/GA10/2022).
文摘Anthropogenic activity-induced sinkholes pose a serious threat to building safety and human life nowadays.Real-time detection and early warning of sinkhole formation are a key and urgent problem in urban areas.This paper presents an experimental study to evaluate the feasibility of fiber optic strain sensing nerves in sinkhole monitoring.Combining the artificial neural network(ANN)and particle image velocimetry(PIV)techniques,a series of model tests have been performed to explore the relationship between strain measurements and sinkhole development and to establish a conversion model from strain data to ground settlements.It is demonstrated that the failure mechanism of the soil above the sinkhole developed from a triangle failure plane to a vertical failure plane with increasing collapse volume.Meanwhile,the soil-embedded fiber optic strain sensing nerves allowed deformation monitoring of the ground soil in real time.Furthermore,the characteristics of the measured strain profiles indicate the locations of sinkholes and the associated shear bands.Based on the strain data,the ANN model predicts the ground settlement well.Additionally,micro-anchored fiber optic cables have been proven to increase the soil-to-fiber strain transfer efficiency for large deformation monitoring of ground collapse.
基金supported by the National Institutes of Health grant R01 AR075037 and R01 AR56212.
文摘Three-dimensional (3D) printing technology is driving forward the progresses of various engineering fields, including tissue engineering. However, the pristine 3D-printed scaffolds usually lack robust functions in stimulating desired activity for varied regeneration applications. In this study, we combined the two-dimensional (2D) hetero-nanostructures and immuno-regulative interleukin-4 (IL-4) cytokines for the functionalization of 3D-printed scaffolds to achieve a pro-healing immuno-microenvironment for optimized bone injury repair. The 2D hetero-nanostructure consists of graphene oxide (GO) layers, for improved cell adhesion, and black phosphorous (BP) nanosheets, for the continuous release of phosphate ions to stimulate cell growth and osteogenesis. In addition, the 2D hetero-nanolayers facilitated the adsorption of large content of immuno-regulative IL-4 cytokines, which modulated the polarization of macrophages into M2 phenotype. After in vivo implantation in rat, the immuno-functioned 3D-scaffolds achieved in vivo osteo-immunomodulation by building a pro-healing immunological microenvironment for better angiogenesis and osteogenesis in the defect area and thus facilitated bone regeneration. These results demonstrated that the immuno-functionalization of 3D-scaffolds with 2D hetero-nanostructures with secondary loading of immuno-regulative cytokines is an encouraging strategy for improving bone regeneration.
