Effective identification of major histocompatibility complex （MHC） molecules restricted peptides is a critical step in discovering immune epitopes. Although many online servers have been built to predict class Ⅱ MH...Effective identification of major histocompatibility complex （MHC） molecules restricted peptides is a critical step in discovering immune epitopes. Although many online servers have been built to predict class Ⅱ MHC-peptide binding affinity, they have been trained on different datasets, and thus fail in providing a unified comparison of various methods. In this paper, we present our implementation of seven popular predictive methods, namely SMM-align, ARB, SVR-pairwise, Gibbs sampler. ProPred, LP-top2, and MHCPred, on a single web server named BiodMHC （http：//biod.whu.edu.cn/BiodMHC/index.html, the software is available upon request）. Using a standard measure of AUC （Area Under the receiver operating characteristic Curves）, we compare these methods by means of not only cross validation but also prediction on independent test datasets. We find that SMM-align, ProPred, SVR-pairwise, ARB, and Gibbs sampler are the five best-performing methods. For the binding affinity prediction of class Ⅱ MHC-peptide, BiodMHC provides a convenient online platform for researchers to obtain binding information simultaneously using various methods.展开更多
基金supported by the National Nature Science Foundation of China (No.60773010)the Shanghai Committee of Science and Technology, China (No.08DZ2271800 and 09DZ2272800)
文摘Effective identification of major histocompatibility complex （MHC） molecules restricted peptides is a critical step in discovering immune epitopes. Although many online servers have been built to predict class Ⅱ MHC-peptide binding affinity, they have been trained on different datasets, and thus fail in providing a unified comparison of various methods. In this paper, we present our implementation of seven popular predictive methods, namely SMM-align, ARB, SVR-pairwise, Gibbs sampler. ProPred, LP-top2, and MHCPred, on a single web server named BiodMHC （http：//biod.whu.edu.cn/BiodMHC/index.html, the software is available upon request）. Using a standard measure of AUC （Area Under the receiver operating characteristic Curves）, we compare these methods by means of not only cross validation but also prediction on independent test datasets. We find that SMM-align, ProPred, SVR-pairwise, ARB, and Gibbs sampler are the five best-performing methods. For the binding affinity prediction of class Ⅱ MHC-peptide, BiodMHC provides a convenient online platform for researchers to obtain binding information simultaneously using various methods.
