Flower type is the most valuable ornamental trait in floricultural plants,for which the ABCDE model was proposed to explain the identity of each floral organ in flowering plants.The C-class gene AGAMOUS(AG)is responsi...Flower type is the most valuable ornamental trait in floricultural plants,for which the ABCDE model was proposed to explain the identity of each floral organ in flowering plants.The C-class gene AGAMOUS(AG)is responsible for stamen formation and plays an essential role in the double flower phenotype.A previous study in carnation revealed that the mutation in the miR172 binding site of the A-class gene APETALA2(AP2)leads to petal accumulation.And the expression level of AG was reduced significantly in the double flowers compared with that in the single flowers.However,there was no sequence polymorphism detected between AGs isolated from the double flowers and single flowers.Here,we performed AG analysis using single and semi-double flower carnations,and detected several mutations located in the crucial position like the MADS-box domain in the AGs of semi-double flower carnations while no changes were found at the miR172 binding site of AP2.As a result,the expression levels of AGs are reduced in the semi-double flower carnation,which could be caught by the loss function of AGs.Our data proves that AGs mutations are also associated with the semi-double flower formation in carnation,complementing the lack of research about AG-mutationassociated double flower formation in carnation.展开更多
Using sucrose, citric acid and vitamin C as the basic liquid, different concentrations of 6-BA and B9 preservatives were added, and the carnation variety ＇ Master＇ was treated. The results showed that the formula of...Using sucrose, citric acid and vitamin C as the basic liquid, different concentrations of 6-BA and B9 preservatives were added, and the carnation variety ＇ Master＇ was treated. The results showed that the formula of 3% sucrose ＋ 50 mg/L citric acid ＋ 600 mg/L vitamin C ＋ 20 mg/L 6-BA could effectively maintain the water balance of the cut flower, prolong the vase life of the cut flower to 16.4 d, and maximize the flower diameter and the crown height.展开更多
基金funded by the Major Science and Technology Project of Yunnan Provincial Department of Science and Technology(202102AE090052)High-level Talent Introduction Program of Yunnan Province-Industrial Talent Special Project(YNQR-CYRC-2020-004)the Green Food Brand-Build a Special Project(Floriculture)supported by Science and Technology(530000210000000013742)。
文摘Flower type is the most valuable ornamental trait in floricultural plants,for which the ABCDE model was proposed to explain the identity of each floral organ in flowering plants.The C-class gene AGAMOUS(AG)is responsible for stamen formation and plays an essential role in the double flower phenotype.A previous study in carnation revealed that the mutation in the miR172 binding site of the A-class gene APETALA2(AP2)leads to petal accumulation.And the expression level of AG was reduced significantly in the double flowers compared with that in the single flowers.However,there was no sequence polymorphism detected between AGs isolated from the double flowers and single flowers.Here,we performed AG analysis using single and semi-double flower carnations,and detected several mutations located in the crucial position like the MADS-box domain in the AGs of semi-double flower carnations while no changes were found at the miR172 binding site of AP2.As a result,the expression levels of AGs are reduced in the semi-double flower carnation,which could be caught by the loss function of AGs.Our data proves that AGs mutations are also associated with the semi-double flower formation in carnation,complementing the lack of research about AG-mutationassociated double flower formation in carnation.
文摘Using sucrose, citric acid and vitamin C as the basic liquid, different concentrations of 6-BA and B9 preservatives were added, and the carnation variety ＇ Master＇ was treated. The results showed that the formula of 3% sucrose ＋ 50 mg/L citric acid ＋ 600 mg/L vitamin C ＋ 20 mg/L 6-BA could effectively maintain the water balance of the cut flower, prolong the vase life of the cut flower to 16.4 d, and maximize the flower diameter and the crown height.
