With the development of the urban pet industry,suppurative diseases of companion animals are increasing day by day.To explore its associated pathogens,this study was conducted on etilolgical identification from a case...With the development of the urban pet industry,suppurative diseases of companion animals are increasing day by day.To explore its associated pathogens,this study was conducted on etilolgical identification from a case of a canine uterus with pus.Bacteriological detection methods were used to isolate and purify the collected disease materials,such as smear staining,microscopic examination and biochemical identification.Then,16S rRNA molecular sequence analysis was used to identify the isolate.It was found that the morphological features of the isolated strain and the biochemical results were consistent with Enterococcus faecalis.The 16S rRNA of the isolate was also as high as 99% homologous with the reference stains.Drug susceptibility test showed that the isolate was resistant to tetracycline,penicillin G,minocycline,erythromycin,ciprofloxacin,norfloxacin,vancomycin,levofloxacin,clindamycin,polymyxin B,chloramphenicol,compound sulfamethoxazole,and clarithromycin,and only susceptible to oxacillin and nitrofurantoin.These results provided reference for the treatment of this type of multidrug-resistant enterococcal infection.展开更多
基金Supported by College Students’ Innovation and Entrepreneurship Project of Tianjin Agricultural University(201710061193)Natural Science Foundation of Tianjin(07JCYBJC16000)Project of Animal Hospital Affiliated to Tianjin Agricultural University(ZH004903)
文摘With the development of the urban pet industry,suppurative diseases of companion animals are increasing day by day.To explore its associated pathogens,this study was conducted on etilolgical identification from a case of a canine uterus with pus.Bacteriological detection methods were used to isolate and purify the collected disease materials,such as smear staining,microscopic examination and biochemical identification.Then,16S rRNA molecular sequence analysis was used to identify the isolate.It was found that the morphological features of the isolated strain and the biochemical results were consistent with Enterococcus faecalis.The 16S rRNA of the isolate was also as high as 99% homologous with the reference stains.Drug susceptibility test showed that the isolate was resistant to tetracycline,penicillin G,minocycline,erythromycin,ciprofloxacin,norfloxacin,vancomycin,levofloxacin,clindamycin,polymyxin B,chloramphenicol,compound sulfamethoxazole,and clarithromycin,and only susceptible to oxacillin and nitrofurantoin.These results provided reference for the treatment of this type of multidrug-resistant enterococcal infection.
