A new spirostanol saponin presenting strong activity of inducing morphological deformation of Pyricularia oryzae mycelia was isolated from Dioscorea futshauensis R. Kunth by bioactivity-guided fractionation. The struc...A new spirostanol saponin presenting strong activity of inducing morphological deformation of Pyricularia oryzae mycelia was isolated from Dioscorea futshauensis R. Kunth by bioactivity-guided fractionation. The structure was established as (25S)-spirost-5-en-3 beta, 27-diol-3-O- [alpha -L-rhamnopyranosyl (1 -->2)-beta -D glucopyranosyl (1 -->3)]-beta -D-glucopyranoside on the basis of chemical evidences and spectral analysis, especially by 2D-NMR techniques.展开更多
From the dried flower-buds of Panax ginseng C.A. Meyer, a new ndnor dammaranetype triterpene saponin named ginsenoside III was iso1ated. On the basis of spectral and chemical evidence, the structure of the new saponin...From the dried flower-buds of Panax ginseng C.A. Meyer, a new ndnor dammaranetype triterpene saponin named ginsenoside III was iso1ated. On the basis of spectral and chemical evidence, the structure of the new saponin was elucidated as 3 -O- [β-D -glucopyranosyl (1→2 ) - βD- glucopyranosyl] - 20-O-β-D-glucopyranosyl 3 β, 12β- 20(S) -trihydroxydammar- 25 - en- 24-one.展开更多
A new triterpenoid saponin, 21 β-angeloylprotoaescigenin-3β-O- [α-L-rhamnopyranosyl- （1-3）]β-D-glucopyranosiduronic acid 1, was isolated from the seeds of Aesculus assamica Griff. Its structure was elucidated on...A new triterpenoid saponin, 21 β-angeloylprotoaescigenin-3β-O- [α-L-rhamnopyranosyl- （1-3）]β-D-glucopyranosiduronic acid 1, was isolated from the seeds of Aesculus assamica Griff. Its structure was elucidated on the basis of spectroscopic data analysis.展开更多
A new furostanol saponin presenting moderate bioactivity of inducing morphological deformation of Pyricularia oryzae mycelia was isolated from Dioscorea futshauensis R.Kunth by bioactivity-guided fractionation. The s...A new furostanol saponin presenting moderate bioactivity of inducing morphological deformation of Pyricularia oryzae mycelia was isolated from Dioscorea futshauensis R.Kunth by bioactivity-guided fractionation. The structure was established as 26-O-b-D-glucopyranosyl-3b, 26-diol-23(S)-methoxyl-(25R)-furost-5,20 (22)-diene-3-O-[a-L-rhamnopyranosyl (12)-b-D- glucopyranosyl (13)]-b-D-glucopyranoside on the basis of chemical evidencesand spectral analysis, especially by 2D-NMR techniques.展开更多
Two new furostanol saponins,named macrostemonoside E(1)and F(2),were isolated from the bulbs of Allium macrostemon Bunge (Liliaceae),their structures were established oy spectroscopic analysis and chemical evidences.B...Two new furostanol saponins,named macrostemonoside E(1)and F(2),were isolated from the bulbs of Allium macrostemon Bunge (Liliaceae),their structures were established oy spectroscopic analysis and chemical evidences.Both possessed strong inhibitory activity on human platelet aggregation.展开更多
A new triterpenoid saponin was isolated by bioactivity-guided isolation from 揋ualou-xiebai-baijiu-tang?consisting of Fructus trichosanthisi and Bulbus allii macrostemi. Its structure was determined as 3-O-b-D-galact...A new triterpenoid saponin was isolated by bioactivity-guided isolation from 揋ualou-xiebai-baijiu-tang?consisting of Fructus trichosanthisi and Bulbus allii macrostemi. Its structure was determined as 3-O-b-D-galactopyranosylhederagenin 28-O-b-D-xylopyranosyl (16)- b-D-galactopyranosyl ester by means of chemical evidences and spectral analysis.展开更多
The dot-immunobinding assay was applied to investigate the characteristics of chicken egg yolk antibodies. This method of assay was proved to be a rapid and simple method to demonstrate and characterize the egg-yolk a...The dot-immunobinding assay was applied to investigate the characteristics of chicken egg yolk antibodies. This method of assay was proved to be a rapid and simple method to demonstrate and characterize the egg-yolk antibody IgY in comparison with the traditional ELISA assay. By using the BandScan software, the gray scale value of dots and the background could be determined. According to the intensity of dots (gray scale value) compared to the standard sample of 10μg, how much IgY remained can be determined in a shorter time.展开更多
A new antifungal antibiotics, WA, was isolated from the secondary metabolites of soil fungus strain 38, and its structure was elucidated as 4-(2-hydroxy-3-butynoxy)benzoic acid by means of the spectral data (H-1-and C...A new antifungal antibiotics, WA, was isolated from the secondary metabolites of soil fungus strain 38, and its structure was elucidated as 4-(2-hydroxy-3-butynoxy)benzoic acid by means of the spectral data (H-1-and C-13-NMR, DEFT, H-1-H-1 COSY, H-1-C-13 COSY, HMBC).展开更多
WE, a new antifungal antibiotic, was isolated from the soil fungus strain 38 against Pyricularia oryzae, and its structure was elucidated as 2-[(E)-3,5-dimethyl-3,4-dihydroxy-1-eptylenyl]-4-chloro-5-oxo-6-methyl-6,7-d...WE, a new antifungal antibiotic, was isolated from the soil fungus strain 38 against Pyricularia oryzae, and its structure was elucidated as 2-[(E)-3,5-dimethyl-3,4-dihydroxy-1-eptylenyl]-4-chloro-5-oxo-6-methyl-6,7-dihydroxy on the basis of H-1-NMR, C-13-NMR, DEFT, HMQC, H-1-H-13 COSY, HMBC spectra.展开更多
A new triterpenoid saponin.3 β-O-{ β-D-glucopyranosyl-（1—4）-[ β-D-glucopyranosyl-[1-2）]- α-L-arabinopyranosyl}-16α, 28-dihydroxyolean-12-en-30-oic acid 30-O- β-D-glucopyranosyl ester（ardisicrenoside N.1）,t...A new triterpenoid saponin.3 β-O-{ β-D-glucopyranosyl-（1—4）-[ β-D-glucopyranosyl-[1-2）]- α-L-arabinopyranosyl}-16α, 28-dihydroxyolean-12-en-30-oic acid 30-O- β-D-glucopyranosyl ester（ardisicrenoside N.1）,together with two known saponins, ardisicrenoside C（2） and D（3）,were isolated from the roots of Ardisia crenata Sim.Their structures were elucidated by extensive spectral analysis and chemical evidences.Saponins 1 showed cytotoxicity against MCI-7 and NCI-H460 cancer cell lines at 11.0μmol/L and 22.1μmol/L in vitro.展开更多
文摘A new spirostanol saponin presenting strong activity of inducing morphological deformation of Pyricularia oryzae mycelia was isolated from Dioscorea futshauensis R. Kunth by bioactivity-guided fractionation. The structure was established as (25S)-spirost-5-en-3 beta, 27-diol-3-O- [alpha -L-rhamnopyranosyl (1 -->2)-beta -D glucopyranosyl (1 -->3)]-beta -D-glucopyranoside on the basis of chemical evidences and spectral analysis, especially by 2D-NMR techniques.
文摘From the dried flower-buds of Panax ginseng C.A. Meyer, a new ndnor dammaranetype triterpene saponin named ginsenoside III was iso1ated. On the basis of spectral and chemical evidence, the structure of the new saponin was elucidated as 3 -O- [β-D -glucopyranosyl (1→2 ) - βD- glucopyranosyl] - 20-O-β-D-glucopyranosyl 3 β, 12β- 20(S) -trihydroxydammar- 25 - en- 24-one.
文摘A new triterpenoid saponin, 21 β-angeloylprotoaescigenin-3β-O- [α-L-rhamnopyranosyl- （1-3）]β-D-glucopyranosiduronic acid 1, was isolated from the seeds of Aesculus assamica Griff. Its structure was elucidated on the basis of spectroscopic data analysis.
基金We appreciate the kind help of Prof. Qishi Sun in Shenyang Pharmaceutical University for his identification of the plant material. Thanks are also extended to Prof. Naili Wang and Doctor Feng Qiu for their advices and assistance.References
文摘A new furostanol saponin presenting moderate bioactivity of inducing morphological deformation of Pyricularia oryzae mycelia was isolated from Dioscorea futshauensis R.Kunth by bioactivity-guided fractionation. The structure was established as 26-O-b-D-glucopyranosyl-3b, 26-diol-23(S)-methoxyl-(25R)-furost-5,20 (22)-diene-3-O-[a-L-rhamnopyranosyl (12)-b-D- glucopyranosyl (13)]-b-D-glucopyranoside on the basis of chemical evidencesand spectral analysis, especially by 2D-NMR techniques.
基金The project supported by the National Natural Science Foundation of China
文摘Two new furostanol saponins,named macrostemonoside E(1)and F(2),were isolated from the bulbs of Allium macrostemon Bunge (Liliaceae),their structures were established oy spectroscopic analysis and chemical evidences.Both possessed strong inhibitory activity on human platelet aggregation.
文摘A new triterpenoid saponin was isolated by bioactivity-guided isolation from 揋ualou-xiebai-baijiu-tang?consisting of Fructus trichosanthisi and Bulbus allii macrostemi. Its structure was determined as 3-O-b-D-galactopyranosylhederagenin 28-O-b-D-xylopyranosyl (16)- b-D-galactopyranosyl ester by means of chemical evidences and spectral analysis.
文摘The dot-immunobinding assay was applied to investigate the characteristics of chicken egg yolk antibodies. This method of assay was proved to be a rapid and simple method to demonstrate and characterize the egg-yolk antibody IgY in comparison with the traditional ELISA assay. By using the BandScan software, the gray scale value of dots and the background could be determined. According to the intensity of dots (gray scale value) compared to the standard sample of 10μg, how much IgY remained can be determined in a shorter time.
文摘A new antifungal antibiotics, WA, was isolated from the secondary metabolites of soil fungus strain 38, and its structure was elucidated as 4-(2-hydroxy-3-butynoxy)benzoic acid by means of the spectral data (H-1-and C-13-NMR, DEFT, H-1-H-1 COSY, H-1-C-13 COSY, HMBC).
文摘WE, a new antifungal antibiotic, was isolated from the soil fungus strain 38 against Pyricularia oryzae, and its structure was elucidated as 2-[(E)-3,5-dimethyl-3,4-dihydroxy-1-eptylenyl]-4-chloro-5-oxo-6-methyl-6,7-dihydroxy on the basis of H-1-NMR, C-13-NMR, DEFT, HMQC, H-1-H-13 COSY, HMBC spectra.
文摘A new triterpenoid saponin.3 β-O-{ β-D-glucopyranosyl-（1—4）-[ β-D-glucopyranosyl-[1-2）]- α-L-arabinopyranosyl}-16α, 28-dihydroxyolean-12-en-30-oic acid 30-O- β-D-glucopyranosyl ester（ardisicrenoside N.1）,together with two known saponins, ardisicrenoside C（2） and D（3）,were isolated from the roots of Ardisia crenata Sim.Their structures were elucidated by extensive spectral analysis and chemical evidences.Saponins 1 showed cytotoxicity against MCI-7 and NCI-H460 cancer cell lines at 11.0μmol/L and 22.1μmol/L in vitro.
