DNA adduction with nicotine-derived nitrosamine, 4-(methylnitrosamino)-1-(3- pyridy1)-1 -butanone (NNK) in mice at doses equivalent to human exposure has been studied using 14C-label by accelerator mass spectrometry(A...DNA adduction with nicotine-derived nitrosamine, 4-(methylnitrosamino)-1-(3- pyridy1)-1 -butanone (NNK) in mice at doses equivalent to human exposure has been studied using 14C-label by accelerator mass spectrometry(AMS). A detection limit of DNA adducts of 1 adduct Per 1010 nucleotides has been achieved. This sensitivity of AMS measurement is 3 or more orders of magnitude improved than that of radioimmunoassay.Formation of DNA adducts is linearly dependent on dose down to an exposure of 100 ng Per kg of body weight .The ultimate cheeical form of carcinogen NNK binding to DNA is speculated.展开更多
文摘DNA adduction with nicotine-derived nitrosamine, 4-(methylnitrosamino)-1-(3- pyridy1)-1 -butanone (NNK) in mice at doses equivalent to human exposure has been studied using 14C-label by accelerator mass spectrometry(AMS). A detection limit of DNA adducts of 1 adduct Per 1010 nucleotides has been achieved. This sensitivity of AMS measurement is 3 or more orders of magnitude improved than that of radioimmunoassay.Formation of DNA adducts is linearly dependent on dose down to an exposure of 100 ng Per kg of body weight .The ultimate cheeical form of carcinogen NNK binding to DNA is speculated.
