Dual-atom catalysts(DACs) afford promising potential for oxygen reduction electrocatalysis due to their high atomic efficiency and high intrinsic activity.However,precise construction of dual-atom sites remains a chal...Dual-atom catalysts(DACs) afford promising potential for oxygen reduction electrocatalysis due to their high atomic efficiency and high intrinsic activity.However,precise construction of dual-atom sites remains a challenge.In this work,a post-modification strategy is proposed to precisely fabricate DACs for oxygen reduction electrocatalysis.Concretely,a secondary metal precursor is introduced to the primary single-atom sites to introduce direct metal-metal interaction,which ensures the formation of desired atom pair structure during the subsequent pyrolysis process and allows for successful construction of DACs.The as-prepared FeCo-NC DAC exhibits superior oxygen reduction electrocatalytic activity with a half-wave potential of 0,91 V vs.reversible hydrogen electrode.Zn-air batteries equipped with the FeCo-NC DAC demonstrate higher peak power density than those with the Pt/C benchmark.More importantly,this post-modification strategy is demonstrated universal to achieve a variety of dual-atom sites.This work presents an effective synthesis methodology for precise construction of catalytic materials and propels their applications in energy-related devices.展开更多
As one of the main active components of Dendrobium catenatum, alkaloids have high medicinal value. The physicochemicalproperties, conserved domains and motifs, phylogenetic analysis, and cis-acting elements of the gen...As one of the main active components of Dendrobium catenatum, alkaloids have high medicinal value. The physicochemicalproperties, conserved domains and motifs, phylogenetic analysis, and cis-acting elements of the genefamily members in the alkaloid biosynthesis pathway of D. catenatum were analyzed by bioinformatics, and theexpression of the genes in different years and tissues was analyzed by qRT-PCR. There are 16 gene families,including 25 genes, in the D. catenatum alkaloid biosynthesis pathway. The analysis of conserved domains andmotifs showed that the types, quantities, and orders of domains and motifs were similar among members ofthe same family, but there were significant differences among families. Phylogenetic analysis indicated that thegene family members showed some evolutionary conservation. Cis-acting element analysis revealed that therewere a large number of light-responsive elements and MYB (v-myb avian myeloblastosis viral oncogene homolog)-related elements in these genes. qRT-PCR showed that expressions of gene family members involved in alkaloidsynthesis were different in different years and tissues of D. catenatum. This study provides a theoretical basisfor further exploration of the regulatory mechanisms of these genes in the alkaloid biosynthesis of D. catenatum.展开更多
Peroxiredoxin 1(PRDX1)participates in tumor cell proliferation,apoptosis,migration,invasion,and the epithelial-to-mesenchymal transition(EMT).This study aimed to investigate the effect of PRDX1 on the EMT of airway ep...Peroxiredoxin 1(PRDX1)participates in tumor cell proliferation,apoptosis,migration,invasion,and the epithelial-to-mesenchymal transition(EMT).This study aimed to investigate the effect of PRDX1 on the EMT of airway epithelial cells stimulated with lipopolysaccharide(LPS)and transforming growth factor-beta 1(TGF-β1).PRDX1 overexpression significantly increased the proliferation and migration of human bronchial epithelial(BEAS-2B)cells,reduced cell apoptosis(p<0.01),and induced EMT and collagen deposition by upregulating the expression of the matrix metallopeptidase(MMP)2,MMP9,α-smooth muscle actin(α-SMA),N-cadherin,vimentin and twist proteins and inhibiting E-cadherin expression(p<0.05).PRDX1 overexpression promoted TGF-β1-mediated inhibition of cell proliferation and migration and significantly enhanced the TGF-β1-induced EMT and collagen synthesis(p<0.05).Knockdown of PRDX1 inhibited cell proliferation,migration,EMT,and collagen synthesis(p<0.01),reversed LPS-mediated inhibition of cell proliferation and migration,and significantly suppressed LPS-induced EMT and collagen synthesis(p<0.01).The result indicating that PRDX1 may be involved in LPS/TGF-1-induced EMT and collagen synthesis in human bronchial epithelial cells.展开更多
The booming wearable/portable electronic devices industry has stimulated the progress of supporting flexible energy storage devices.Excellent performance of flexible devices not only requires the component units of ea...The booming wearable/portable electronic devices industry has stimulated the progress of supporting flexible energy storage devices.Excellent performance of flexible devices not only requires the component units of each device to maintain the original performance under external forces,but also demands the overall device to be flexible in response to external fields.However,flexible energy storage devices inevitably occur mechanical damages(extrusion,impact,vibration)/electrical damages(overcharge,over-discharge,external short circuit)during longterm complex deformation conditions,causing serious performance degradation and safety risks.Inspired by the healing phenomenon of nature,endowing energy storage devices with self-healing capability has become a promising strategy to effectively improve the durability and functionality of devices.Herein,this review systematically summarizes the latest progress in intrinsic self-healing chemistry for energy storage devices.Firstly,the main intrinsic self-healing mechanism is introduced.Then,the research situation of electrodes,electrolytes,artificial interface layers and integrated devices based on intrinsic self-healing and advanced characterization technology is reviewed.Finally,the current challenges and perspective are provided.We believe this critical review will contribute to the development of intrinsic self-healing chemistry in the flexible energy storage field.展开更多
As a low cost non-staple food resource,the high-viscosity paste and poor gel-forming ability of tapioca starch limit its industrial application.Herein,molasses hydrocolloids that is a by-product of the sugar refining ...As a low cost non-staple food resource,the high-viscosity paste and poor gel-forming ability of tapioca starch limit its industrial application.Herein,molasses hydrocolloids that is a by-product of the sugar refining process was applied as a blending modifier to reduce the viscosity of tapioca starch paste.The test results of paste and rheological properties show that molasses hydrocolloids exhibited a good physical viscosity-reducing effect on tapioca starch paste.The irregular network structure and high K^(+)/Ca^(2+)ion contents of molasses hydrocolloids exerted wrapping,adhesion,barrier,and hydration effects on starch,leading to the reduction of viscosity.The scanning electron microscope images and textural analysis demonstrated that this strategy also improve the structure of tapioca starch gel and enhanced its puncture strength by 75.46%.This work shows the great potential of molasses hydrocolloids as a lowcost and desirable material for the viscosity reduction of tapioca starch.展开更多
In valley photonic crystals, topological edge states can be gained by breaking the spatial inversion symmetry without breaking time-reversal symmetry or creating pseudo-spin structures, making highly unidirectional li...In valley photonic crystals, topological edge states can be gained by breaking the spatial inversion symmetry without breaking time-reversal symmetry or creating pseudo-spin structures, making highly unidirectional light transmission easy to achieve. This paper presents a novel physical model of a hexagonal-star valley photonic crystal. Simulations based on the finite element method(FEM) are performed to investigate the propagation of TM polarized mode and its application to ring resonators. The results show that such a topologically triangular ring resonator exhibits an optimum quality factor Q of about 1.25×104, and Q has a maximum value for both frequency and the cavity length L. Our findings are expected to have significant implications for developing topological lasers and wavelength division multiplexers.展开更多
Concerns about China's energy security have escalated because of the country's high dependency on oil and gas imports, so it is necessary to calculate the availability of domestic oil and gas resources and Chi...Concerns about China's energy security have escalated because of the country's high dependency on oil and gas imports, so it is necessary to calculate the availability of domestic oil and gas resources and China's ability to obtain foreign energy through trade. In this work,the calculation was done by using the energy return on investment(EROI) method. The results showed that the EROI_(stnd)(i.e., standard EROI) of China's oil and gas extraction decreased from approximately 17.3:1 in 1986 to 8.4:1 in 2003, but it increased to 12.2:1 in 2013. From a company-level perspective, the EROI_(stnd)differed for different companies and was in the range of(8–12):1. The EROI_(2,d)(EROI considering energy outputs after processed and direct energy inputs) for different companies was in the range of(3–7):1. The EROI of imported oil(EROI_(IO))declined from 14.8:1 in 1998 to approximately 4.8:1 in 2014, and the EROI of imported natural gas(EROI_(ING))declined from 16.7:1 in 2009 to 8.6:1 in 2014. In 2015, the EROI_(IO) and EROI_(ING) showed a slight increase due to decreasing import prices. In general, this paper suggests that from a net energy perspective, it has become more difficult for China to obtain oil and gas from both domestic production and imports. China is experiencing an EROI decline, which demonstrates the risk in the use of unsustainable fossil resources.展开更多
Proton exchange membrane fuel cells(PEMFC)have attracted much attention because of their high energy conversion efficiency,high power density and zero emission of pollutants.However,the high cost of the cathode platin...Proton exchange membrane fuel cells(PEMFC)have attracted much attention because of their high energy conversion efficiency,high power density and zero emission of pollutants.However,the high cost of the cathode platinum group metal(PGM)catalysts creates a barrier for the large-scale application of PEMFC.Tremendous efforts have been devoted to the development of low-cost PGM-free catalysts,especially the Fe-N-C catalysts,to replace the expensive PGM catalysts.However,the characterization methods and evaluation standards of the catalysts varies,which is not conducive to the comparison of PGM-free catalysts.U.S.Department of energy(DOE)is the only authority that specifies the testing standards and activity targets for PGM-free catalysts.In this review,the major breakthroughs of Fe-N-C catalysts are outlined with the reference of DOE standards and targets.The preparation and characteristics of these highly active Fe-N-C catalysts are briefly introduced.Moreover,the efforts on improving the mass transfer and the durability issue of Fe-N-C fuel cell are discussed.Finally,the prospective directions concerning the comprehensive evaluation of the Fe-N-C catalysts are proposed.展开更多
AIM: To investigate the effect of gambogic acid(GA) on apoptosis in the HT-29 human colon cancer cell line. METHODS: H-29 cells were used for in vitro experiments in this study. Relative cell viability was assessed us...AIM: To investigate the effect of gambogic acid(GA) on apoptosis in the HT-29 human colon cancer cell line. METHODS: H-29 cells were used for in vitro experiments in this study. Relative cell viability was assessed using MTT assays. Cell apoptosis was detected by terminal deoxynucleotidyl transferase d UTP nick end labeling and Hoechst 33342 staining, and quantified by flow cytometry. Cellular ultrastructure was observed by transmission electron microscopy. Real-time PCR and Western blot analyses were used to evaluate gene and protein expression levels. For in vivo experiments, BALB/c nude mice received subcutaneous injections of HT-29 cells in the right armpit. When well-established xenografts were palpable with a tumor size of 75 mm3, mice were randomly assigned to a vehicle(negative) control, positive control or GA treatment group(n = 6 each). The animals in the treatment group received one of three dosages of GA(in saline; 5, 10 or 20 mg/kg) via the caudal vein twice weekly, whereas animals in the negative and positive control groups were given equal volumes of 0.9% saline or 10 mg/kg docetaxel, respectively, via the caudal vein once weekly. RESULTS: The cell viability assay showed that GA inhibited proliferation of HT-29 cells in a dose- and time-dependent manner after treatment with GA(0.00, 0.31, 0.62, 1.25, 2.50, 5.00 or 10.00 μmol/L) for 24, 48 or 72 h. After 48 h, the percentage of apoptotic cells in cells treated with 0.00, 1.25, 2.50 and 5.00 μmol/L GA was 1.4% ± 0.3%, 9.8% ± 1.2%, 25.7% ± 3.3% and 49.3% ± 5.8%, respectively. Ultrastructural analysis of HT-29 cells treated for 48 h with 2.5μmol/L GA revealed apoptotic bodies and condensed and fragmented nuclei. Levels of caspase-8,-9 and-3 m RNAs were significantly increased after treatment with GA(1.25, 2.50 or 5.00 μmol/L) for 48 h(P < 0.05 for all). Protein levels of apoptosis-related factors Fas, Fas L, FADD, cytochrome c, and Apaf-1 were increased in GA-treated cells, whereas levels of pro-caspase-8,-9 and-3 were significantly decreased(P < 0.05 for all). Furthermore, GA significantly and dose-dependently inhibited the growth of HT-29 tumors in a mouse xenograft model(P < 0.05).CONCLUSION: GA inhibits HT-29 proliferation via induction of apoptosis. The anti-cancer effects are likely mediated by death receptor(extrinsic) and mitochondrial(intrinsic) pathways.展开更多
In this work,we carried out three-dimensional(3D)atomic-scale study on the growth characteristics of{10-12}twin in magnesium(Mg).The study was performed by MD simulations on Mg single crystals with an initial twin nuc...In this work,we carried out three-dimensional(3D)atomic-scale study on the growth characteristics of{10-12}twin in magnesium(Mg).The study was performed by MD simulations on Mg single crystals with an initial twin nucleus structures.A detailed atomistic analysis reveals that a stabilized 3D{10-12}twin nucleus is bounded by basal/prismatic(BP)interfaces,prismatic/basal(PB)interfaces,and{10-11}interfaces.Later,a{10-12}twin boundary(TB)occurs at the junction of the BP and PB interface with the growth of the twin nucleus.In this process,two twinning mechanisms are involved:a pure-shuffle mechanism in which{10-11}interface migration along the[11-20]direction is mediated by atomic shuffle,and a glide-shuffle mechanism in which BP/PB and{10-12}TB movements are realized by the migration of disconnections along the relevant interfaces.In addition,we systematically investigate the stress state associated with the activation of twinning,aiming to discover the intrinsic relationship of the elastic stress field to twin growth on an atomic scale.The results suggest that the elastic stress in the matrix is an important driving force for twin growth,much similar to what stress does for a crack.In addition,it is rather remarkable that the{10-11}interface has a greater ability to migrate than other interfaces,and this is thought to be a main factor for the rapid growth of a{10-12}twin.展开更多
The studies and development of coal seam gas(CSG) have been conducted for more than 30 years in China, but few of China's CSG projects have achieved large-scale commercial success; faced with the boom of shale gas...The studies and development of coal seam gas(CSG) have been conducted for more than 30 years in China, but few of China's CSG projects have achieved large-scale commercial success; faced with the boom of shale gas, some investors are beginning to lose patience and confidence in CSG. China currently faces the following question: Should the government continue to vigorously support the development of the CSG industry? To provide a reference for policy makers and investors, this paper calculates the EROI_(stnd)[a standardized energy return on investment(EROI) method], EROI_(ide)(the maximum theoretical EROI), EROI_(3,i)(EROI considering the energy investment in transport), and EROI_(3,1+e)(EROI with environmental inputs) of a single vertical CSG well in the Fanzhuang CSG project in the Qinshui Basin. The energy payback time(EPT) and the greenhouse gas(GHG) emissions of the CSG systems are also calculated. The results show that over a 15-year lifetime, EROI_(stnd), EROI_(ide), EROI_(3,1), and EROI_(3,1+e)are expected to deliver EROIs of approximately11:1, 20:1, 7:1, and 6:1, respectively. The EPT within different boundaries is no more than 2 years, and the life-cycle GHG emissions are approximately 18.8 million kg CO_2 equivalent. The relatively high EROI and short EPT indicate that the government should take more positive measures to promote the development of the CSG industry.展开更多
The toxic dyestuff's from printing and dyeing wastewater have caused serious damages to the ecological environ-ment,thus exploring effective methods to remove them having become a key topic.Here,a series of biocha...The toxic dyestuff's from printing and dyeing wastewater have caused serious damages to the ecological environ-ment,thus exploring effective methods to remove them having become a key topic.Here,a series of biochar sam-ples were synthesized form kenaf to adsorb methylene blue(MB),which was acted as the dye representative for the test of adsorption capacity due to the presence of abundant double bond and aroma tic heterocyclic ring.By tuning the raw materials and pyrolysis temperature,a super adsorption capacity about 164.21 mg·g^(-1) was obtained over the biochar that pyrolyzed at 700℃ with the kenaf fiber as raw material Through the physical adsorption,elemental analysis,FTIR spectra and NH_(3)-TPD,it was found the high surface area and pore volume of biochar played a key role in the adsorption of MB,and the acidic sites would also assist the adsorption process.Besides,the adsorption kinetic model was ftted and calculated,implying the MB physically adsorbed on the bio-char rapidly and then occurred chemical adsorption on the acidic sites.In addition,through KBC700 recycling experiments,it was found that kenaf biochar had a good binding force to MB,which effectively avoided secondary pollution.This work provides important insights for the adsorption mechanism of MB by biochar,also offers some guidance for the further synthesis of biochar from various biomass.展开更多
The mechanism and effectiveness of welding distortion mitigation by static thermal tensioning were investigated by both finite element analysis and experiments. It shows that preset thermal tensioning can reduce longi...The mechanism and effectiveness of welding distortion mitigation by static thermal tensioning were investigated by both finite element analysis and experiments. It shows that preset thermal tensioning can reduce longitudinal plastic compression remained in the weld and its adjacent zone significantly, so decrease the longitudinal residual stress and the susceptibility to welding distortion.展开更多
Fractional quantum Hall systems are often described by model wave functions,which are the ground states of pure systems with short-range interaction.A primary example is the Laughlin wave function,which supports Abeli...Fractional quantum Hall systems are often described by model wave functions,which are the ground states of pure systems with short-range interaction.A primary example is the Laughlin wave function,which supports Abelian quasiparticles with fractionalized charge.In the presence of disorder,the wave function of the ground state is expected to deviate from the Laughlin form.We study the disorder-driven colla.pse of the quantum Hall state by analyzing the evolution of the ground state and the single-quasihole state.In particular,we demonstrate that the quasihole tunneling amplitude can signal the fractional quantum Hall phase to insulator transition.展开更多
Vocabulary researches play an important role in language teaching.In recent decades,lexical complexity has drawn great attention by researchers and scholars.A large number of empirical studies have been conducted to f...Vocabulary researches play an important role in language teaching.In recent decades,lexical complexity has drawn great attention by researchers and scholars.A large number of empirical studies have been conducted to find out whether correlations exist between writing quality and lexical complexity in written texts.In the present study,299 English learners participated in the experiment from a comprehensive university.The data were timed CE texts translated by these learners.This study intends to answer the two questions with the assistance of such tools as LCA and SPSS.After analyzing the data with 9 indices from LCA,the results of this study are as follows:1.Chinese students’translated texts gain a high lexical density,which shows that content words can account for a large percentage in narrative translations.In the lexical sophistication measures,LS1 and LS2 do not differ greatly while CVS1 gets the greatest fluctuation.Among lexical variation indices,NDW is generally high.2.All indices in lexical variation and lexical sophistication show a high positive correlation with translation quality,while lexical density bears a negative relationship with translation quality.The findings of the current study indicate students’vocabulary use in translation and provide implications for English teaching,especially translation and vocabulary teaching.展开更多
The electrochemical oxidation of biomass molecules coupling with hydrogen production is a promising strategy to obtain both green energy and value-added chemicals;however,this strategy is limited by the competing oxyg...The electrochemical oxidation of biomass molecules coupling with hydrogen production is a promising strategy to obtain both green energy and value-added chemicals;however,this strategy is limited by the competing oxygen evolution reactions and high energy consumption.Herein,we report a hierarchical CoNi layered double hydroxides(LDHs)electrocatalyst with abundant Ni vacancies for the efficient anodic oxidation of 5-hydroxymethylfurfural(HMF)and cathodic hydrogen evolution.The unique hierarchical nanosheet structure and Ni vacancies provide outstanding activity and selectivity toward several biomass molecules because of the finely regulated electronic structure and highly-exposed active sites.In particular,a high faradaic efficiency(FE)at a high current density(99%at 100 mA cm^(-2))is achieved for HMF oxidation,and a two-electrode electrolyzer is assembled based on the Ni vacancies-enriched LDH,which realized a continuous synthesis of highly-pure 2,5-furandicarboxylic acid products with high yields(95%)and FE(90%).展开更多
The ongoing coronavirus disease 2019(COVID-19)pandemic caused more than 96 million infections and over 2 million deaths worldwide so far.However,there is no approved vaccine available for severe acute respiratory synd...The ongoing coronavirus disease 2019(COVID-19)pandemic caused more than 96 million infections and over 2 million deaths worldwide so far.However,there is no approved vaccine available for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the disease causative agent.Vaccine is the most effective approach to eradicate a pathogen.The tests of safety and efficacy in animals are pivotal for developing a vaccine and before the vaccine is applied to human populations.Here we evaluated the safety,immunogenicity,and efficacy of an inactivated vaccine based on the whole viral particles in human ACE2 transgenic mouse and in non-human primates.Our data showed that the inactivated vaccine successfully induced SARS-CoV-2-specific neutralizing antibodies in mice and non-human primates,and subsequently provided partial(in low dose)or full(in high dose)protection of challenge in the tested animals.In addition,passive serum transferred from vaccine-immunized mice could also provide full protection from SARS-CoV-2 infection in mice.These results warranted positive outcomes in future clinical trials in humans.展开更多
Due to the high specific surface area,abundant nitrogen and micropores,ZIF-8 is a commonly used precursor for preparing high performance Fe-N-C catalysts.However,the Zn element is inevitably remained in the prepared F...Due to the high specific surface area,abundant nitrogen and micropores,ZIF-8 is a commonly used precursor for preparing high performance Fe-N-C catalysts.However,the Zn element is inevitably remained in the prepared Fe-N-C catalyst.Whether the residual Zn element affects the catalytic activity and active site center of the Fe-N-C catalyst caused widespread curiosity,but has not been studied yet.Herein,we built several Fe,Zn,and N co-doped graphene models to investigate the effect of Zn atoms on the electrocatalytic performance of Fe-N-C catalysts by using density functional theory method.The calculation results show that all the calculated Fe-Zn-N_(x) structures are thermodynamically stable due to the negative formation energies and relative stabilities.The active sites around Fe and Zn atoms in the structure of Fe-Zn-N_(6)(III)show the lowest oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)overpotentials of 0.38 and 0.43 V,respectively.The bridge site of Fe-Zn in Fe-Zn-N_(5) shows the lowest η^(HER) of−0.26 V.A few structures with a better activity than that of FeN_(4) or ZnN_(4) are attributed to the synergistic effects between Fe and Zn atoms.The calculated ORR reaction pathways on Fe-Zn-N6(III)show that H_(2)O is the final product and the ORR mechanism on the catalyst would be a four-electron process,and the existence of Zn element in the Fe-N-C catalysts plays a key role in reducing the ORR activation energy barrier.The results are helpful for the deep understand of high-performance Fe-N-C catalysts.展开更多
基金This work was supported by the National Natural Science Foundation of China(22279008 and 22109082)the Beijing Institute of Technology Research Fund Program for Young Scholarsthe Tsinghua University Initiative Scientific Research Program。
文摘Dual-atom catalysts(DACs) afford promising potential for oxygen reduction electrocatalysis due to their high atomic efficiency and high intrinsic activity.However,precise construction of dual-atom sites remains a challenge.In this work,a post-modification strategy is proposed to precisely fabricate DACs for oxygen reduction electrocatalysis.Concretely,a secondary metal precursor is introduced to the primary single-atom sites to introduce direct metal-metal interaction,which ensures the formation of desired atom pair structure during the subsequent pyrolysis process and allows for successful construction of DACs.The as-prepared FeCo-NC DAC exhibits superior oxygen reduction electrocatalytic activity with a half-wave potential of 0,91 V vs.reversible hydrogen electrode.Zn-air batteries equipped with the FeCo-NC DAC demonstrate higher peak power density than those with the Pt/C benchmark.More importantly,this post-modification strategy is demonstrated universal to achieve a variety of dual-atom sites.This work presents an effective synthesis methodology for precise construction of catalytic materials and propels their applications in energy-related devices.
基金the Forestry Science and Technology Innovation and promotion Project of Jiangsu Province‘Long-Term Research Base of Forest and Wetland Positioning Monitoring in Jiangsu Province’(Grant No.LYKJ[2020]21)Natural Science Foundation of Jiangsu Province,China(Grant No.BK20210800)+1 种基金the National Natural Science Foundation of China(Grant Nos.32001341 and 32202523)Jiangsu Agriculture Science and Technology Innovation Fund(Grant No.CX(21)3047).
文摘As one of the main active components of Dendrobium catenatum, alkaloids have high medicinal value. The physicochemicalproperties, conserved domains and motifs, phylogenetic analysis, and cis-acting elements of the genefamily members in the alkaloid biosynthesis pathway of D. catenatum were analyzed by bioinformatics, and theexpression of the genes in different years and tissues was analyzed by qRT-PCR. There are 16 gene families,including 25 genes, in the D. catenatum alkaloid biosynthesis pathway. The analysis of conserved domains andmotifs showed that the types, quantities, and orders of domains and motifs were similar among members ofthe same family, but there were significant differences among families. Phylogenetic analysis indicated that thegene family members showed some evolutionary conservation. Cis-acting element analysis revealed that therewere a large number of light-responsive elements and MYB (v-myb avian myeloblastosis viral oncogene homolog)-related elements in these genes. qRT-PCR showed that expressions of gene family members involved in alkaloidsynthesis were different in different years and tissues of D. catenatum. This study provides a theoretical basisfor further exploration of the regulatory mechanisms of these genes in the alkaloid biosynthesis of D. catenatum.
基金The study was supported by the Fujian Provincial Science and Technology Department of the Project Fund under Grant(Grant No.2015J01573)the Medical Innovation Projects of Fujian Province’s Health and Scientific Research Talent Training Project under Grant(Grant No.2019-CXB-25).
文摘Peroxiredoxin 1(PRDX1)participates in tumor cell proliferation,apoptosis,migration,invasion,and the epithelial-to-mesenchymal transition(EMT).This study aimed to investigate the effect of PRDX1 on the EMT of airway epithelial cells stimulated with lipopolysaccharide(LPS)and transforming growth factor-beta 1(TGF-β1).PRDX1 overexpression significantly increased the proliferation and migration of human bronchial epithelial(BEAS-2B)cells,reduced cell apoptosis(p<0.01),and induced EMT and collagen deposition by upregulating the expression of the matrix metallopeptidase(MMP)2,MMP9,α-smooth muscle actin(α-SMA),N-cadherin,vimentin and twist proteins and inhibiting E-cadherin expression(p<0.05).PRDX1 overexpression promoted TGF-β1-mediated inhibition of cell proliferation and migration and significantly enhanced the TGF-β1-induced EMT and collagen synthesis(p<0.05).Knockdown of PRDX1 inhibited cell proliferation,migration,EMT,and collagen synthesis(p<0.01),reversed LPS-mediated inhibition of cell proliferation and migration,and significantly suppressed LPS-induced EMT and collagen synthesis(p<0.01).The result indicating that PRDX1 may be involved in LPS/TGF-1-induced EMT and collagen synthesis in human bronchial epithelial cells.
基金supported by China Postdoctoral Science Foundation(2022M710951,2022TQ0087)Postdoctoral Science Foundation of Heilongjiang Province(LBH-Z22111)。
文摘The booming wearable/portable electronic devices industry has stimulated the progress of supporting flexible energy storage devices.Excellent performance of flexible devices not only requires the component units of each device to maintain the original performance under external forces,but also demands the overall device to be flexible in response to external fields.However,flexible energy storage devices inevitably occur mechanical damages(extrusion,impact,vibration)/electrical damages(overcharge,over-discharge,external short circuit)during longterm complex deformation conditions,causing serious performance degradation and safety risks.Inspired by the healing phenomenon of nature,endowing energy storage devices with self-healing capability has become a promising strategy to effectively improve the durability and functionality of devices.Herein,this review systematically summarizes the latest progress in intrinsic self-healing chemistry for energy storage devices.Firstly,the main intrinsic self-healing mechanism is introduced.Then,the research situation of electrodes,electrolytes,artificial interface layers and integrated devices based on intrinsic self-healing and advanced characterization technology is reviewed.Finally,the current challenges and perspective are provided.We believe this critical review will contribute to the development of intrinsic self-healing chemistry in the flexible energy storage field.
基金supported by the National Natural Science Foundation of China(U21B2097)the National Key Research and Development Program of China(2018YFA0901500)the Jiangsu Postdoctoral Research Foundation(2019K242)。
文摘As a low cost non-staple food resource,the high-viscosity paste and poor gel-forming ability of tapioca starch limit its industrial application.Herein,molasses hydrocolloids that is a by-product of the sugar refining process was applied as a blending modifier to reduce the viscosity of tapioca starch paste.The test results of paste and rheological properties show that molasses hydrocolloids exhibited a good physical viscosity-reducing effect on tapioca starch paste.The irregular network structure and high K^(+)/Ca^(2+)ion contents of molasses hydrocolloids exerted wrapping,adhesion,barrier,and hydration effects on starch,leading to the reduction of viscosity.The scanning electron microscope images and textural analysis demonstrated that this strategy also improve the structure of tapioca starch gel and enhanced its puncture strength by 75.46%.This work shows the great potential of molasses hydrocolloids as a lowcost and desirable material for the viscosity reduction of tapioca starch.
基金Project supported by the National Natural Science Foundation of China (Grant No. 1217040857)。
文摘In valley photonic crystals, topological edge states can be gained by breaking the spatial inversion symmetry without breaking time-reversal symmetry or creating pseudo-spin structures, making highly unidirectional light transmission easy to achieve. This paper presents a novel physical model of a hexagonal-star valley photonic crystal. Simulations based on the finite element method(FEM) are performed to investigate the propagation of TM polarized mode and its application to ring resonators. The results show that such a topologically triangular ring resonator exhibits an optimum quality factor Q of about 1.25×104, and Q has a maximum value for both frequency and the cavity length L. Our findings are expected to have significant implications for developing topological lasers and wavelength division multiplexers.
基金supported by the National Natural Science Foundation of China(No.71273277)the Philosophy and Social Sciences Major Research Project of the Ministry of Education(No.11JZD048)
文摘Concerns about China's energy security have escalated because of the country's high dependency on oil and gas imports, so it is necessary to calculate the availability of domestic oil and gas resources and China's ability to obtain foreign energy through trade. In this work,the calculation was done by using the energy return on investment(EROI) method. The results showed that the EROI_(stnd)(i.e., standard EROI) of China's oil and gas extraction decreased from approximately 17.3:1 in 1986 to 8.4:1 in 2003, but it increased to 12.2:1 in 2013. From a company-level perspective, the EROI_(stnd)differed for different companies and was in the range of(8–12):1. The EROI_(2,d)(EROI considering energy outputs after processed and direct energy inputs) for different companies was in the range of(3–7):1. The EROI of imported oil(EROI_(IO))declined from 14.8:1 in 1998 to approximately 4.8:1 in 2014, and the EROI of imported natural gas(EROI_(ING))declined from 16.7:1 in 2009 to 8.6:1 in 2014. In 2015, the EROI_(IO) and EROI_(ING) showed a slight increase due to decreasing import prices. In general, this paper suggests that from a net energy perspective, it has become more difficult for China to obtain oil and gas from both domestic production and imports. China is experiencing an EROI decline, which demonstrates the risk in the use of unsustainable fossil resources.
基金supported by the National Thousand Talents Plan of Chinathe National Natural Science Foundation of China(Grant Nos.21673014 and U1766216)+1 种基金the 111 project(B17002)funded by the Ministry of Education of Chinathe Fundamental Research Funds for the Central Universities of China
文摘Proton exchange membrane fuel cells(PEMFC)have attracted much attention because of their high energy conversion efficiency,high power density and zero emission of pollutants.However,the high cost of the cathode platinum group metal(PGM)catalysts creates a barrier for the large-scale application of PEMFC.Tremendous efforts have been devoted to the development of low-cost PGM-free catalysts,especially the Fe-N-C catalysts,to replace the expensive PGM catalysts.However,the characterization methods and evaluation standards of the catalysts varies,which is not conducive to the comparison of PGM-free catalysts.U.S.Department of energy(DOE)is the only authority that specifies the testing standards and activity targets for PGM-free catalysts.In this review,the major breakthroughs of Fe-N-C catalysts are outlined with the reference of DOE standards and targets.The preparation and characteristics of these highly active Fe-N-C catalysts are briefly introduced.Moreover,the efforts on improving the mass transfer and the durability issue of Fe-N-C fuel cell are discussed.Finally,the prospective directions concerning the comprehensive evaluation of the Fe-N-C catalysts are proposed.
文摘AIM: To investigate the effect of gambogic acid(GA) on apoptosis in the HT-29 human colon cancer cell line. METHODS: H-29 cells were used for in vitro experiments in this study. Relative cell viability was assessed using MTT assays. Cell apoptosis was detected by terminal deoxynucleotidyl transferase d UTP nick end labeling and Hoechst 33342 staining, and quantified by flow cytometry. Cellular ultrastructure was observed by transmission electron microscopy. Real-time PCR and Western blot analyses were used to evaluate gene and protein expression levels. For in vivo experiments, BALB/c nude mice received subcutaneous injections of HT-29 cells in the right armpit. When well-established xenografts were palpable with a tumor size of 75 mm3, mice were randomly assigned to a vehicle(negative) control, positive control or GA treatment group(n = 6 each). The animals in the treatment group received one of three dosages of GA(in saline; 5, 10 or 20 mg/kg) via the caudal vein twice weekly, whereas animals in the negative and positive control groups were given equal volumes of 0.9% saline or 10 mg/kg docetaxel, respectively, via the caudal vein once weekly. RESULTS: The cell viability assay showed that GA inhibited proliferation of HT-29 cells in a dose- and time-dependent manner after treatment with GA(0.00, 0.31, 0.62, 1.25, 2.50, 5.00 or 10.00 μmol/L) for 24, 48 or 72 h. After 48 h, the percentage of apoptotic cells in cells treated with 0.00, 1.25, 2.50 and 5.00 μmol/L GA was 1.4% ± 0.3%, 9.8% ± 1.2%, 25.7% ± 3.3% and 49.3% ± 5.8%, respectively. Ultrastructural analysis of HT-29 cells treated for 48 h with 2.5μmol/L GA revealed apoptotic bodies and condensed and fragmented nuclei. Levels of caspase-8,-9 and-3 m RNAs were significantly increased after treatment with GA(1.25, 2.50 or 5.00 μmol/L) for 48 h(P < 0.05 for all). Protein levels of apoptosis-related factors Fas, Fas L, FADD, cytochrome c, and Apaf-1 were increased in GA-treated cells, whereas levels of pro-caspase-8,-9 and-3 were significantly decreased(P < 0.05 for all). Furthermore, GA significantly and dose-dependently inhibited the growth of HT-29 tumors in a mouse xenograft model(P < 0.05).CONCLUSION: GA inhibits HT-29 proliferation via induction of apoptosis. The anti-cancer effects are likely mediated by death receptor(extrinsic) and mitochondrial(intrinsic) pathways.
基金The authors are grateful for the support from the National Natural Science Foundation of China(No.51471038)the Fundamental Research Funds for the Central Universities(No.2018CDPTCG0001/42)the National special support program for high-level personnel recruitment.
文摘In this work,we carried out three-dimensional(3D)atomic-scale study on the growth characteristics of{10-12}twin in magnesium(Mg).The study was performed by MD simulations on Mg single crystals with an initial twin nucleus structures.A detailed atomistic analysis reveals that a stabilized 3D{10-12}twin nucleus is bounded by basal/prismatic(BP)interfaces,prismatic/basal(PB)interfaces,and{10-11}interfaces.Later,a{10-12}twin boundary(TB)occurs at the junction of the BP and PB interface with the growth of the twin nucleus.In this process,two twinning mechanisms are involved:a pure-shuffle mechanism in which{10-11}interface migration along the[11-20]direction is mediated by atomic shuffle,and a glide-shuffle mechanism in which BP/PB and{10-12}TB movements are realized by the migration of disconnections along the relevant interfaces.In addition,we systematically investigate the stress state associated with the activation of twinning,aiming to discover the intrinsic relationship of the elastic stress field to twin growth on an atomic scale.The results suggest that the elastic stress in the matrix is an important driving force for twin growth,much similar to what stress does for a crack.In addition,it is rather remarkable that the{10-11}interface has a greater ability to migrate than other interfaces,and this is thought to be a main factor for the rapid growth of a{10-12}twin.
基金supported by the National Natural Science Foundation of China (No. 71273277, 71722003, 71690244)the Philosophy and Social Sciences Major Research Project of the Ministry of Education (No. 11JZD048)the National Key R&D Program (2016YFC0208901)
文摘The studies and development of coal seam gas(CSG) have been conducted for more than 30 years in China, but few of China's CSG projects have achieved large-scale commercial success; faced with the boom of shale gas, some investors are beginning to lose patience and confidence in CSG. China currently faces the following question: Should the government continue to vigorously support the development of the CSG industry? To provide a reference for policy makers and investors, this paper calculates the EROI_(stnd)[a standardized energy return on investment(EROI) method], EROI_(ide)(the maximum theoretical EROI), EROI_(3,i)(EROI considering the energy investment in transport), and EROI_(3,1+e)(EROI with environmental inputs) of a single vertical CSG well in the Fanzhuang CSG project in the Qinshui Basin. The energy payback time(EPT) and the greenhouse gas(GHG) emissions of the CSG systems are also calculated. The results show that over a 15-year lifetime, EROI_(stnd), EROI_(ide), EROI_(3,1), and EROI_(3,1+e)are expected to deliver EROIs of approximately11:1, 20:1, 7:1, and 6:1, respectively. The EPT within different boundaries is no more than 2 years, and the life-cycle GHG emissions are approximately 18.8 million kg CO_2 equivalent. The relatively high EROI and short EPT indicate that the government should take more positive measures to promote the development of the CSG industry.
基金This work was supported by National Natural Science Foundation of China(51903131)Natural Science Foundation of Shandong Province(ZR2019QEM007)+2 种基金Key Research and Development Program of Shandong Province(2020CXGC011101)State Key Laboratory of Bio-Fibers and Eco-Textiles(Qingdao University)(ZKT16 and ZKT21)Special Foundation of“Taishan Scholar”Construction Program(ts20190932).
文摘The toxic dyestuff's from printing and dyeing wastewater have caused serious damages to the ecological environ-ment,thus exploring effective methods to remove them having become a key topic.Here,a series of biochar sam-ples were synthesized form kenaf to adsorb methylene blue(MB),which was acted as the dye representative for the test of adsorption capacity due to the presence of abundant double bond and aroma tic heterocyclic ring.By tuning the raw materials and pyrolysis temperature,a super adsorption capacity about 164.21 mg·g^(-1) was obtained over the biochar that pyrolyzed at 700℃ with the kenaf fiber as raw material Through the physical adsorption,elemental analysis,FTIR spectra and NH_(3)-TPD,it was found the high surface area and pore volume of biochar played a key role in the adsorption of MB,and the acidic sites would also assist the adsorption process.Besides,the adsorption kinetic model was ftted and calculated,implying the MB physically adsorbed on the bio-char rapidly and then occurred chemical adsorption on the acidic sites.In addition,through KBC700 recycling experiments,it was found that kenaf biochar had a good binding force to MB,which effectively avoided secondary pollution.This work provides important insights for the adsorption mechanism of MB by biochar,also offers some guidance for the further synthesis of biochar from various biomass.
文摘The mechanism and effectiveness of welding distortion mitigation by static thermal tensioning were investigated by both finite element analysis and experiments. It shows that preset thermal tensioning can reduce longitudinal plastic compression remained in the weld and its adjacent zone significantly, so decrease the longitudinal residual stress and the susceptibility to welding distortion.
基金Supported by the National Natural Science Foundation of China under Grant No 11674282the Strategic Priority Research Program of Chinese Academy of Sciences under Grant No XDB28000000the National Basic Research Program of China under Grant No 2015CB921101
文摘Fractional quantum Hall systems are often described by model wave functions,which are the ground states of pure systems with short-range interaction.A primary example is the Laughlin wave function,which supports Abelian quasiparticles with fractionalized charge.In the presence of disorder,the wave function of the ground state is expected to deviate from the Laughlin form.We study the disorder-driven colla.pse of the quantum Hall state by analyzing the evolution of the ground state and the single-quasihole state.In particular,we demonstrate that the quasihole tunneling amplitude can signal the fractional quantum Hall phase to insulator transition.
文摘Vocabulary researches play an important role in language teaching.In recent decades,lexical complexity has drawn great attention by researchers and scholars.A large number of empirical studies have been conducted to find out whether correlations exist between writing quality and lexical complexity in written texts.In the present study,299 English learners participated in the experiment from a comprehensive university.The data were timed CE texts translated by these learners.This study intends to answer the two questions with the assistance of such tools as LCA and SPSS.After analyzing the data with 9 indices from LCA,the results of this study are as follows:1.Chinese students’translated texts gain a high lexical density,which shows that content words can account for a large percentage in narrative translations.In the lexical sophistication measures,LS1 and LS2 do not differ greatly while CVS1 gets the greatest fluctuation.Among lexical variation indices,NDW is generally high.2.All indices in lexical variation and lexical sophistication show a high positive correlation with translation quality,while lexical density bears a negative relationship with translation quality.The findings of the current study indicate students’vocabulary use in translation and provide implications for English teaching,especially translation and vocabulary teaching.
基金This work was supported by the National Natural Science Foundation of China(22090031,22090030,21922501 and 21871021)Project funded by China Postdoctoral Science Foundation(2021M690319).
文摘The electrochemical oxidation of biomass molecules coupling with hydrogen production is a promising strategy to obtain both green energy and value-added chemicals;however,this strategy is limited by the competing oxygen evolution reactions and high energy consumption.Herein,we report a hierarchical CoNi layered double hydroxides(LDHs)electrocatalyst with abundant Ni vacancies for the efficient anodic oxidation of 5-hydroxymethylfurfural(HMF)and cathodic hydrogen evolution.The unique hierarchical nanosheet structure and Ni vacancies provide outstanding activity and selectivity toward several biomass molecules because of the finely regulated electronic structure and highly-exposed active sites.In particular,a high faradaic efficiency(FE)at a high current density(99%at 100 mA cm^(-2))is achieved for HMF oxidation,and a two-electrode electrolyzer is assembled based on the Ni vacancies-enriched LDH,which realized a continuous synthesis of highly-pure 2,5-furandicarboxylic acid products with high yields(95%)and FE(90%).
基金supported by the National Key R&D Program of China(2020YFC0842000 to Z.M.Yuan and 2020YFC0842100 to C.Shan)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB29010101 to Z.L.Shi)+1 种基金the China Natural Science Foundation(82041013 to P.Zhou)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(CAS)(2019328 to X.L.Yang)。
文摘The ongoing coronavirus disease 2019(COVID-19)pandemic caused more than 96 million infections and over 2 million deaths worldwide so far.However,there is no approved vaccine available for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the disease causative agent.Vaccine is the most effective approach to eradicate a pathogen.The tests of safety and efficacy in animals are pivotal for developing a vaccine and before the vaccine is applied to human populations.Here we evaluated the safety,immunogenicity,and efficacy of an inactivated vaccine based on the whole viral particles in human ACE2 transgenic mouse and in non-human primates.Our data showed that the inactivated vaccine successfully induced SARS-CoV-2-specific neutralizing antibodies in mice and non-human primates,and subsequently provided partial(in low dose)or full(in high dose)protection of challenge in the tested animals.In addition,passive serum transferred from vaccine-immunized mice could also provide full protection from SARS-CoV-2 infection in mice.These results warranted positive outcomes in future clinical trials in humans.
基金The authors acknowledge the financial support from the National Natural Science Foundation of China under Grant Nos.21673014 and 21975010This research is supported by the high-performance computing(HPC)resources at Beihang University.The work is carried out at LvLiang Cloud Computing Center of China,and the calculations are performed on TianHe-2.
文摘Due to the high specific surface area,abundant nitrogen and micropores,ZIF-8 is a commonly used precursor for preparing high performance Fe-N-C catalysts.However,the Zn element is inevitably remained in the prepared Fe-N-C catalyst.Whether the residual Zn element affects the catalytic activity and active site center of the Fe-N-C catalyst caused widespread curiosity,but has not been studied yet.Herein,we built several Fe,Zn,and N co-doped graphene models to investigate the effect of Zn atoms on the electrocatalytic performance of Fe-N-C catalysts by using density functional theory method.The calculation results show that all the calculated Fe-Zn-N_(x) structures are thermodynamically stable due to the negative formation energies and relative stabilities.The active sites around Fe and Zn atoms in the structure of Fe-Zn-N_(6)(III)show the lowest oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)overpotentials of 0.38 and 0.43 V,respectively.The bridge site of Fe-Zn in Fe-Zn-N_(5) shows the lowest η^(HER) of−0.26 V.A few structures with a better activity than that of FeN_(4) or ZnN_(4) are attributed to the synergistic effects between Fe and Zn atoms.The calculated ORR reaction pathways on Fe-Zn-N6(III)show that H_(2)O is the final product and the ORR mechanism on the catalyst would be a four-electron process,and the existence of Zn element in the Fe-N-C catalysts plays a key role in reducing the ORR activation energy barrier.The results are helpful for the deep understand of high-performance Fe-N-C catalysts.