Application of Horizontal Well Seismic Geo-Steering Technology in XX Block Development

During the development phase horizontal wells are very efficient way to improve the production in the deep coal bed methane. The 8# coal seam in the XX block on the eastern edge of the Ordos Basin has challenges such as deep burial depth (>2000 m), thin coal sweet spot (3 m), and significant short-distance structural fluctuations. The challenges caused a high risk of missing targets and running out of the target layers, as well as difficulties in cementing and completion due to uneven well trajectories. To address these challenges, this paper focuses on solving the issues through detailed precise geological modeling, optimized trajectory design, and accurate seismic geology Steerable Drilling. 1) Based on reasonable velocity field construction and Time-Depth transformation, a precise directional model is constructed using the layer-by-layer approximation principle with reference to marker layers, improving the accuracy of the target spot and avoiding premature or delayed entry into the target;2) Based on a precise geological model, the dip angle of the strata ahead and the development of sweet spots are clearly defined, enabling optimized trajectory design for horizontal wells;3) Using “1 + N” dynamic modeling to update the geological model in real-time during the drilling process, and actively guide the drill bit through the horizontal segment smoothly by using multi-information judgment of the drill bit position. The actual drilling of 80 completed horizontal wells in this area show: That this approach effectively ensures the smooth trajectory and high-quality drilling rate of the horizontal well in the coal seam, providing a basis for subsequent hydraulic fracturing and increasing single-well production. At the same time, it has certain potential value and significance for similar coalbed methane developments under similar geological conditions.

Living environment shaped residents’willingness to pay for ecosystem services in Yangtze River Middle Reaches Megalopolis,China

Profound understanding on the diversity of local residents’willingness to pay(WTP)for ecosystem services(ESs)may aid evaluation of policy impacts by uncovering trade-offs and synergies for achieving sustainable environmental resource management.However,the reasons for the spatial preference heterogeneity of WTP are still unclear.In this study,an extensive survey with 4,580 residents in the Yangtze River Middle Reaches Megalopolis(YRMRM),central China,was carried out to investigate the public’s WTP to maintain and improve ESs.We identified key influencing factors and explored how they shaped the preference heterogeneity in WTP by random forest model and surface fitting method.Our results showed that 81.4% of residents reported WTP for ESs greater than zero.Water supply,waste treatment,food supply and raw material supply were the preferred ESs for residents.Living environment factors(i.e.,extent of farmland,water and forest near the residence)were found as the key drivers of WTP.Proximity to farmland,water and forests resulted in high WTP values for a series of specific ESs.The reasons for the heterogeneity of WTP are(1)the difference in proximity to farmland,water and forest(i.e.,the contributor to ESs),(2)the divergence of local residents’preferences for ESs.This study disclosed the heterogeneity and causes of public preferences in YRMRM,which could help policy and decision makers to develop eco-compensation initiatives towards conservative and sustainable use of the environmental resources.

西部地区十二省份数字经济发展水平测度与碳排放影响的关系研究

随着“东数西算”工程全面启动,本文采用一种合适的、可量化的方法测度当前较我国东、中部地区数字经济发展水平存在较大差异的西部地区发展现状。一方面,本文选用2014—2020年国家统计局统计年鉴、第三产业统计年鉴、信息产业统计年鉴及目标省份统计年鉴中部分数字经济可替代性关键指标进行扩展和修正,采用改进的TOPSIS方法对西部地区十二省份的数字经济发展水平进行测度和评价;另一方面,通过构建计量模型探讨上述西部地区十二省份数字经济发展对碳排放的影响关系及其作用机制。结果显示,西部地区各省数字经济发展存在“数字鸿沟”现象,同时西部地区数字经济发展对碳排放的影响并未呈现出显著的倒“U”型非线性关系,而是处于一种发展早期所呈现的正线性相关关系。因此,本文为决策机构制定和实施西部地区数字经济与环境协调发展的相关政策提供了思路和建议,仅供参考。

Ex vivo liver resection and auto-transplantation as an alternative for the treatment of liver malignancies: Progress and challenges

Hepatectomy is still the major curative treatment for patients with liver malignancies.However,it is still a big challenge to remove the tumors in the central posterior area,especially if their location involves the retrohepatic inferior vena cava and hepatic veins.Ex vivo liver resection and auto-transplantation(ELRA),a hybrid technique of the traditional liver resection and transplantation,has brought new hope to these patients and therefore becomes a valid alternative to liver transplantation.Due to its technical difficulty,ELRA is still concentrated in a few hepatobiliary centers that have experienced surgeons in both liver resection and liver transplantation.The efficacy and safety of this technique has already been demonstrated in the treatment of benign liver diseases,especially in the advanced alveolar echinococcosis.Recently,the application of ELRA for liver malignances has gained more attention.However,standardization of clinical practice norms and international consensus are still lacking.The prognostic impact in these oncologic patients also needs further evaluation.In this review,we summarized the principles and recent progresses on ELRA.

禽致病性大肠埃希氏菌生物被膜形成的调控机制研究进展

禽致病性大肠埃希氏菌能够引起禽类感染性细菌病,给禽类养殖业造成了重大的经济损失,并严重限制了禽类养殖业的健康发展。为了了解禽致病性大肠埃希氏菌的感染机制,论文简述了生物被膜的形成过程及其危害。细菌生物被膜的形成不但增强细菌对抗菌药物的耐药性,而且导致宿主持续和反复性感染,同时还很难被预防、控制和清除。论文还分析了普遍存在于各类细菌中的重要调控系统——群体感应、双组份系统和第二信使的信号转导途径及其调控机制,并阐述了群体感应、双组份系统和第二信使以及两系统间的互作网络对细菌生物被膜的影响。因此,了解多系统共同调控生物被膜形成的分子机制或许可以作为破坏生物被膜形成的新策略,从而为控制由生物被膜引起的感染和抗菌药物耐药性提供研究方向。

基于细胞实验及网络药理学探究人参五味子汤对肺炎的治疗作用

为探究人参五味子汤对肺炎的治疗作用,通过细胞实验考察人参五味子汤对炎症细胞因子的表达影响;利用数据库获取人参五味子汤各味中药的成分、靶点及肺炎靶点,将两类靶点取交集;通过String平台建立PPI网络,并获得核心靶点,再通过Metascape数据库分析GO和KEGG,最后利用AutoDock软件进行分子对接,并进行能量最小化处理。结果表明,人参五味子汤显著抑制炎症细胞因子的表达;筛选得到27个活性成分,38个核心靶点;分子对接表明活性成分与核心靶点结合较好。研究通过细胞实验证实人参五味子汤具有抗炎活性的基础上,通过网络药理学结合分子对接预测得到治疗肺炎的活性成分,为人参五味子汤治疗肺炎的作用机制研究奠定基础。

汽车天窗系统进排水特性试验研究

为解决汽车天窗漏水问题,通过简化汽车天窗排水总成系统,构建天窗排水管路物理模型并对管路设计进行建模推导,得到管路最大排水能力的理论计算公式,并搭建天窗试验台架,基于理论分析结果,通过试验测量各参数及管路零件对排水管路排水能力的影响程度,并对比理论结果和试验结果。针对实车天窗的进水特性进行试验研究,分析不同车辆状态下天窗进排水量分布特点,发现排水管水流量分布随车辆倾斜角度的不同而不同,水平状态下车辆前部排水量高于后部,建议管路设计时考虑车辆使用的坡度工况,增加前排水管的设计余量。

Targeting TrkB–PSD-95 coupling to mitigate neurological disorders

Tropomyosin receptor kinase B(TrkB)signaling plays a pivotal role in dendritic growth and dendritic spine formation to promote learning and memory.The activity-dependent release of brain-derived neurotrophic factor at synapses binds to pre-or postsynaptic TrkB resulting in the strengthening of synapses,reflected by long-term potentiation.Postsynaptically,the association of postsynaptic density protein-95 with TrkB enhances phospholipase Cγ-Ca^(2+)/calmodulin-dependent protein kinaseⅡand phosphatidylinositol 3-kinase-mechanistic target of rapamycin signaling required for long-term potentiation.In this review,we discuss TrkB-postsynaptic density protein-95 coupling as a promising strategy to magnify brain-derived neurotrophic factor signaling towards the development of novel therapeutics for specific neurological disorders.A reduction of TrkB signaling has been observed in neurodegenerative disorders,such as Alzheimer's disease and Huntington's disease,and enhancement of postsynaptic density protein-95 association with TrkB signaling could mitigate the observed deficiency of neuronal connectivity in schizophrenia and depression.Treatment with brain-derived neurotrophic factor is problematic,due to poor pharmacokinetics,low brain penetration,and side effects resulting from activation of the p75 neurotrophin receptor or the truncated TrkB.T1 isoform.Although TrkB agonists and antibodies that activate TrkB are being intensively investigated,they cannot distinguish the multiple human TrkB splicing isoforms or cell type-specific functions.Targeting TrkB–postsynaptic density protein-95 coupling provides an alternative approach to specifically boost TrkB signaling at localized synaptic sites versus global stimulation that risks many adverse side effects.

Heterogeneous expression of stearoyl-acyl carrier protein desaturase genes SAD1 and SAD2 from Linum usitatissimum enhances seed oleic acid accumulation and seedling cold and drought tolerance in Brassica napus

Flax(Linum usitatissimum L.)is a versatile crop and its seeds are a major source of unsaturated fatty acids.Stearoyl-acyl carrier protein desaturase(SAD)is a dehydrogenase enzyme that plays a key role in oleic acid biosynthesis as well as responses to biotic and abiotic stresses.However,the function of SAD orthologs from L.usitatissimum has not been assessed.Here,we found that two LuSAD genes,LuSAD1 and LuSAD2,are present in the genome of L.usitatissimum cultivar‘Longya 10’.Heterogeneous expression of either LuSAD1 or LuSAD2 in Arabidopsis thaliana resulted in higher contents of total fatty acids and oleic acid in the seeds.Interestingly,ectopic expression of LuSAD2 in A.thaliana caused altered plant architecture.Similarly,the overexpression of either LuSAD1 or LuSAD2 in Brassica napus also resulted in increased contents of total fatty acids and oleic acid in the seeds.Furthermore,we demonstrated that either LuSAD1 or LuSAD2 enhances seedling resistance to cold and drought stresses by improving antioxidant enzyme activity and nonenzymatic antioxidant levels,as well as reducing membrane damage.These findings not only broaden our knowledge of the LuSAD functions in plants,but also offer promising targets for improving the quantity and quality of oil,and the abiotic stress tolerance of oil-producing crops,through molecular manipulation.

Reorganization of 3D genome architecture provides insights into pathogenesis of early fatty liver disease in laying hens

Background Fatty liver disease causes huge economic losses in the poultry industry due to its high occurrence and lethality rate.Three-dimensional(3D)chromatin architecture takes part in disease processing by regulating tran-scriptional reprogramming.The study is carried out to investigate the alterations of hepatic 3D genome and H3K27ac profiling in early fatty liver(FLS)and reveal their effect on hepatic transcriptional reprogramming in laying hens.Results Results show that FLS model is constructed with obvious phenotypes including hepatic visible lipid deposi-tion as well as higher total triglyceride and cholesterol in serum.A/B compartment switching,topologically associat-ing domain(TAD)and chromatin loop changes are identified by high-throughput/resolution chromosome conforma-tion capture(HiC)technology.Targeted genes of these alternations in hepatic 3D genome organization significantly enrich pathways related to lipid metabolism and hepatic damage.H3K27ac differential peaks and differential expres-sion genes(DEGs)identified through RNA-seq analysis are also enriched in these pathways.Notably,certain DEGs are found to correspond with changes in 3D chromatin structure and H3K27ac binding in their promoters.DNA motif analysis reveals that candidate transcription factors are implicated in regulating transcriptional reprogram-ming.Furthermore,disturbed folate metabolism is observed,as evidenced by lower folate levels and altered enzyme expression.Conclusion Our findings establish a link between transcriptional reprogramming changes and 3D chromatin struc-ture variations during early FLS formation,which provides candidate transcription factors and folate as targets for FLS prevention or treatment.

Optical storage of circular airy beam in atomic vapor

The realization of quantum storage of spatial light field is of great significance to the construction of high-dimensional quantum repeater.In this paper,we experimentally realize the storage and retrieval of circular Airy beams(CABs)by using theΛ-type three-level energy system based on the electromagnetically induced transparency in a hot rubidium atomic vapor cell.The weak probe beam field is modulated with phase distribution of CABs by a spatial light modulator.We store the probe circular Airy beam(CAB)into the rubidium atomic vapor cell and retrieve it after the demanded delay.We quantitatively analyze the storage results and give corresponding theoretical explanations.Moreover,we investigate the autofocusing and self-healing effect of the retrieved CAB,which indicates that the properties and beam shape of CAB maintain well after storage.Our work will have potential applications in the storage of high-dimensional quantum information,and is also useful for improving the channel capacities of quantum internet.

Spatial quantum coherent modulation with perfect hybrid vector vortex beam based on atomic medium

The perfect hybrid vector vortex beam(PHVVB)with helical phase wavefront structure has aroused significant concern in recent years,as its beam waist does not expand with the topological charge(TC).In this work,we investigate the spatial quantum coherent modulation effect with PHVVB based on the atomic medium,and we observe the absorption characteristic of the PHVVB with different TCs under variant magnetic fields.We find that the transmission spectrum linewidth of PHVVB can be effectively maintained regardless of the TC.Still,the width of transmission peaks increases slightly as the beam size expands in hot atomic vapor.This distinctive quantum coherence phenomenon,demonstrated by the interaction of an atomic medium with a hybrid vector-structured beam,might be anticipated to open up new opportunities for quantum coherence modulation and accurate magnetic field measurement.

Understanding the Resilience of Urban Rail Transit:Concepts,Reviews,and Trends

As the scale of urban rail transit(URT)networks expands,the study of URT resilience is essential for safe and efficient operations.This paper presents a comprehensive review of URT resilience and highlights potential trends and directions for future research.First,URT resilience is defined by three primary abilities:absorption,resistance,and recovery,and four properties:robustness,vulnerability,rapidity,and redundancy.Then,the metrics and assessment approaches for URT resilience were summarized.The metrics are divided into three categories:topology-based,characteristic-based,and performance-based,and the assessment methods are divided into four categories:topological,simulation,optimization,and datadriven.Comparisons of various metrics and assessment approaches revealed that the current research trend in URT resilience is increasingly favoring the integration of traditional methods,such as conventional complex network analysis and operations optimization theory,with new techniques like big data and intelligent computing technology,to accurately assess URT resilience.Finally,five potential trends and directions for future research were identified:analyzing resilience based on multisource data,optimizing train diagram in multiple scenarios,accurate response to passenger demand through new technologies,coupling and optimizing passenger and traffic flows,and optimal line design.

Luminescence regulation of Sb^(3+)in 0D hybrid metal halides by hydrogen bond network for optical anti-counterfeiting

The Sb^(3+) doping strategy has been proven to be an effective way to regulate the band gap and improve the photophysical properties of organic-inorganic hybrid metal halides(OIHMHs).However,the emission of Sb^(3+) ions in OIHMHs is primarily confined to the low energy region,resulting in yellow or red emissions.To date,there are few reports about green emission of Sb^(3+)-doped OIHMHs.Here,we present a novel approach for regulating the luminescence of Sb^(3+) ions in 0D C_(10)H_(2)_(2)N_(6)InCl_(7)·H_(2)O via hydrogen bond network,in which water molecules act as agents for hydrogen bonding.Sb^(3+)-doped C_(10)H_(2)2N_(6)InCl_(7)·H_(2)O shows a broadband green emission peaking at 540 nm and a high photoluminescence quantum yield(PLQY)of 80%.It is found that the intense green emission stems from the radiative recombination of the self-trapped excitons(STEs).Upon removal of water molecules with heat,C_(10)H_(2)_(2)N_(6)In_(1-x)Sb_(x)Cl_(7) generates yellow emis-sion,attributed to the breaking of the hydrogen bond network and large structural distortions of excited state.Once water molecules are adsorbed by C_(10)H_(2)_(2)N_(6)In_(1-x)Sb_(x)Cl_(7),it can subsequently emit green light.This water-induced reversible emission switching is successfully used for optical security and information encryption.Our findings expand the under-standing of how the local coordination structure influences the photophysical mechanism in Sb^(3+)-doped metal halides and provide a novel method to control the STEs emission.

Two different mutational types of familial gastrointestinal stromal tumors:Two case reports

BACKGROUND Gastrointestinal stromal tumors(GISTs)are the most common mesenchymal tumors of the gastrointestinal(GI)tract,and cases of GISTs tend to be of the disseminated type,with a global incidence of 10 to 15 cases/million each year.The rarer familial GISTs,which often represent a population,differ in screening,diagnosis,and treatment.Familial GISTs include primary familial GISTs with predominantly KIT/PDGFRA mutations and wild-type GISTs.However,whether the same genetic family has different phenotypes has not been reported.CASE SUMMARY We report two cases of rare GISTs in the same family:A male patient with the V561D mutation in exon 12 of the PDGFRA gene,who has been taking the targeted drug imatinib since undergoing surgery,and a female patient diagnosed with wild-type GIST,who has been taking imatinib for 3 years since undergoing surgery.The favorable prognosis of these patients during the 7-year follow-up period validates the accuracy of our treatment strategy,and we have refined the entire process of diagnosis and treatment of familial GISTs in order to better manage this rare familial disease.CONCLUSION Different mutation types of familial GISTs in the same family are very rare,thus it is very important to make the correct diagnosis and treatment strategies according to the results of molecular detection for the management of familial GISTs.

MULTIFRACTAL ANALYSIS OF CONVERGENCE EXPONENTS FOR PRODUCTS OF CONSECUTIVE PARTIAL QUOTIENTS IN CONTINUED FRACTIONS

For each real number x∈(0,1),let[a_(1)(x),a_(2)(x),…,a_n(x),…]denote its continued fraction expansion.We study the convergence exponent defined byτ(x)=inf{s≥0:∞∑n=1(a_(n)(x)a_(n+1)(x))^(-s)<∞},which reflects the growth rate of the product of two consecutive partial quotients.As a main result,the Hausdorff dimensions of the level sets ofτ(x)are determined.

A Fusion Localization Method Based on Target Measurement Error Feature Complementarity and Its Application

In the multi-radar networking system,aiming at the problem of locating long-distance targets synergistically with difficulty and low accuracy,a dual-station joint positioning method based on the target measurement error feature complementarity is proposed.For dual-station joint positioning,by constructing the target positioning error distribution model and using the complementarity of spatial measurement errors of the same long-distance target,the area with high probability of target existence can be obtained.Then,based on the target distance information,the midpoint of the intersection between the target positioning sphere and the positioning tangent plane can be solved to acquire the target's optimal positioning result.The simulation demonstrates that this method greatly improves the positioning accuracy of target in azimuth direction.Compared with the traditional the dynamic weighted fusion(DWF)algorithm and the filter-based dynamic weighted fusion(FBDWF)algorithm,it not only effectively eliminates the influence of systematic error in the azimuth direction,but also has low computational complexity.Furthermore,for the application scenarios of multi-radar collaborative positioning and multi-sensor data compression filtering in centralized information fusion,it is recommended that using radar with higher ranging accuracy and the lengths of baseline between radars are 20–100 km.

Decoding exercise-induced atomic components and prognostic shifts in endometrial carcinoma through differentially expressed genes

Background:This study aimed to portray the atomic intelligence and prognostic implications of differentially expressed genes and their involvement in biological pathways in endometrial carcinoma,with a specific focus on the impacts of exercise on cancer.Methods:We utilized a multi-faceted approach,including volcano plots,Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses,Venn diagrams,protein-protein interaction networks,Kaplan-Meier survival analysis,Gene Set Variety Analysis,and single-cell transcriptomic analysis.Furthermore,we profiled tumor mutational scenes,assessed the prognostic value of immune-related features,and conducted a comprehensive examination of genetic variations and their impact on tumor mutational burden across different cancer types.Multidimensional genomic interactions and methylation elements were also investigated.Using real-time quantitative PCR and immunofluorescence staining,the effects of B-cell lymphoma 2(BCL2)silencing on TNF-αand caspase-3 gene expression were evaluated.Results:Our study identified a noteworthy number of differentially expressed genes in endometrial carcinoma with potential links to athletic performance traits.BCL2 expression levels were found to be associated with survival outcomes,and its changeability across cancers was related to immune cell infiltration and immune checkpoint gene expression.Single-cell investigations uncovered cellular complexity within tumor microenvironments and critical biological pathways in BCL2-overexpressing cells.The expression flow and mutational effect of BCL2 in endometrial carcinoma were characterized,and the prognostic implications of immune-related features were assessed.Hereditary variations,including copy number variations and their relationship with gene expression and tumor mutational burden,were investigated.Multidimensional genomic transaction highlighted the essential role of regulatory genes in cancer pathogenesis.Silencing of the BCL2 gene significantly inhibited the proliferation of HEC-108 cells and promoted apoptosis,as evidenced by decreased TNF-αgene expression and increased caspase-3 gene expression.Immunofluorescence staining further confirmed these results.Conclusion:This study gives a point-by-point understanding of the atomic intelligence and prognostic implications in endometrial carcinoma and across various other cancers.BCL2’s role as a modulatory factor within the tumor-resistant environment and its potential impact on disease prognosis and response to immunotherapy were underscored.The multidimensional genomic analysis provides insights into the complex interaction between genetic and epigenetic variables in cancer,which may shed light on future therapeutic strategies.This study indicates that silencing the BCL2 gene can significantly inhibit tumor cell proliferation and promote apoptosis through the regulation of the TNF-αand caspase-3 pathways.

Effects of comprehensive nursing with cognitive behavioral therapy in orthodontic osteodilated arch treatment

BACKGROUND This work explored the effects of cognitive behavior therapy(CBT)-based comprehensive nursing intervention(CNI)mode in arch expansion to treat patients with orthodontic osteodilated arch(OOA).AIM To explore the application effect of CBT-based CNI model in orthodontic expansion arch treatment.METHODS Using convenient sampling method,81 patients with OOA were selected and rolled into a control group(Ctrl group,40 cases)and an observation group(Obs group,41 cases).During the treatment,patients in the Ctrl group received routine nursing intervention mode,and the those in the Obs group received CBT mode on the basis of this.Before and after intervention,the incidence of oral mucositis,the mastery rate of correct arch expansion method,self-rating anxiety scale score,soft scale index,and plaque index were compared for patients in different groups.In addition,satisfaction and complications were comparatively analyzed.RESULTS Incidence of oral mucositis in the Obs group was lower(14.6%vs 38.5%),and the mastery rate of correct arch expansion method was obviously higher(90.2%vs 55.0%)was obviously higher(all P<0.05).Meanwhile,the soft scale index and plaque index in the Obs group were much lower(P<0.05).The compliance(90.24%)and satisfaction(95.12%)in the Obs group were greatly higher(P<0.05).CONCLUSION The CBT-based CNI mode greatly improved the mastery rate of correct arch expansion method during arch expansion in treating patients with OOA and enhanced the therapeutic effect of arch expansion and the oral health of patients,improving the patient compliance.

Methods to increase the diagnostic efficiency of endoscopic ultrasound-guided fine-needle aspiration for solid pancreatic lesions:An updated review

Endoscopic ultrasound-guided fine-needle aspiration(EUS-FNA)is a means to procure adequate specimens for histological and cytologic analysis.The ideal EUS-FNA should be safe,accurate,and have a high sample adequacy rate and low adverse events rate.In recent years,many guidelines and trials on EUS-FNA have been published.The purpose of this article is to provide an update on the influence of some of the main factors on the diagnostic efficiency of EUS-FNA as well as a rare but serious complication known as needle tract seeding.