AIM:To investigate the intestinal barrier changes in ratswith CCl_4-induced portal hypertension.METHODS:The permeability of intestinal barrier detectedby Lanthanum as a tracer was evaluated in rats,Bacterialtranslocat...AIM:To investigate the intestinal barrier changes in ratswith CCl_4-induced portal hypertension.METHODS:The permeability of intestinal barrier detectedby Lanthanum as a tracer was evaluated in rats,Bacterialtranslocation and plasma endotoxin were also determined.RESULTS:The incidence of bacterial translocation was85% in rats with CCl_4-induced portal hypertension,whichwas significantly higher than that in control rats(20%,P<0.01).Plasma endotoxin level was significantly higherin experimental group than in control group.Permeabilityof the epithelial mucosa and pathological alteration wereincreased in the ileum and the microvilli became shorterand thinner in rats with portal hypertension.CONCLUSION:Bacterial translocation occurs in ratswith CCl_4-induced portal hypertension and increasedpermeability between epithelial cells contributes to thetranslocation.展开更多
AIM: To investigate the effect of oncolytic adenovirus SG600-IL24 and replication-incompetent adenovirus Ad.IL-24 on hepatocellular carcinoma (HCC) cell lines and normal liver cell line. METHODS: HCC cell lines (HepG2...AIM: To investigate the effect of oncolytic adenovirus SG600-IL24 and replication-incompetent adenovirus Ad.IL-24 on hepatocellular carcinoma (HCC) cell lines and normal liver cell line. METHODS: HCC cell lines (HepG2, Hep3B and MHCC97L) and normal liver cell line (L02) with a different p53 status were infected with SG600-IL24 and Ad.IL-24, respectively. Melanoma differentiation-associated (MDA)-7/interleukin (IL)-24 mRNA and protein expressions in infected cells were detected by reverse transcription-polymerase chain reaction (RT-PCR), enzymelinked immunosorbent assay (ELISA), and Western blotting, respectively. Apoptosis of HCC cells and normal liver cells was detected by cytometric assay with Hoechst33258 staining. 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay was used to investigate proliferation of HCC cells and normal liver cells, and cell cycle was assayed by flow cytometry. RESULTS: RT-PCR, ELISA and Western blotting showed that the exogenous MDA-7/IL-24 gene was highly expressed in cells infected with SG600-IL24. MTT indicated that SG600-IL24 could suppress the growth of HepG2, Hep3B, MHCC97L, with an inhibition rate of 75% ± 2.5%, 85% ± 2.0%, 72% ± 1.8%, respectively (P < 0.01), promote the apoptosis of HepG2, Hep3B, MHCC97L, with an apoptosis rate of 56.59% ± 4.0%, 78.36% ± 3.5%, 43.39% ± 2.5%, respectively (P < 0.01), and block the HCC cell lines in the G2/M phase with a blocking rate of 35.4% ± 4.2%, 47.3% ± 6.2%, 42% ± 5.0%, respectively (P < 0.01) but not the normal liver cell line in a p53-independent manner. CONCLUSION: SG600-IL24 can selectively suppress the proliferation and apoptosis of HCC cell lines in vitro but not normal liver cell line L02 in a p53-independent manner. Compared with Ad.IL-24, SG600-IL24 can significantly enhance the antitumor activity in HCC cell lines.展开更多
文摘AIM:To investigate the intestinal barrier changes in ratswith CCl_4-induced portal hypertension.METHODS:The permeability of intestinal barrier detectedby Lanthanum as a tracer was evaluated in rats,Bacterialtranslocation and plasma endotoxin were also determined.RESULTS:The incidence of bacterial translocation was85% in rats with CCl_4-induced portal hypertension,whichwas significantly higher than that in control rats(20%,P<0.01).Plasma endotoxin level was significantly higherin experimental group than in control group.Permeabilityof the epithelial mucosa and pathological alteration wereincreased in the ileum and the microvilli became shorterand thinner in rats with portal hypertension.CONCLUSION:Bacterial translocation occurs in ratswith CCl_4-induced portal hypertension and increasedpermeability between epithelial cells contributes to thetranslocation.
基金Supported by National Natural Science Foundation of China,No. 30872510Natural Science Foundation of Hubei Province,No. 2008CDB127
文摘AIM: To investigate the effect of oncolytic adenovirus SG600-IL24 and replication-incompetent adenovirus Ad.IL-24 on hepatocellular carcinoma (HCC) cell lines and normal liver cell line. METHODS: HCC cell lines (HepG2, Hep3B and MHCC97L) and normal liver cell line (L02) with a different p53 status were infected with SG600-IL24 and Ad.IL-24, respectively. Melanoma differentiation-associated (MDA)-7/interleukin (IL)-24 mRNA and protein expressions in infected cells were detected by reverse transcription-polymerase chain reaction (RT-PCR), enzymelinked immunosorbent assay (ELISA), and Western blotting, respectively. Apoptosis of HCC cells and normal liver cells was detected by cytometric assay with Hoechst33258 staining. 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay was used to investigate proliferation of HCC cells and normal liver cells, and cell cycle was assayed by flow cytometry. RESULTS: RT-PCR, ELISA and Western blotting showed that the exogenous MDA-7/IL-24 gene was highly expressed in cells infected with SG600-IL24. MTT indicated that SG600-IL24 could suppress the growth of HepG2, Hep3B, MHCC97L, with an inhibition rate of 75% ± 2.5%, 85% ± 2.0%, 72% ± 1.8%, respectively (P < 0.01), promote the apoptosis of HepG2, Hep3B, MHCC97L, with an apoptosis rate of 56.59% ± 4.0%, 78.36% ± 3.5%, 43.39% ± 2.5%, respectively (P < 0.01), and block the HCC cell lines in the G2/M phase with a blocking rate of 35.4% ± 4.2%, 47.3% ± 6.2%, 42% ± 5.0%, respectively (P < 0.01) but not the normal liver cell line in a p53-independent manner. CONCLUSION: SG600-IL24 can selectively suppress the proliferation and apoptosis of HCC cell lines in vitro but not normal liver cell line L02 in a p53-independent manner. Compared with Ad.IL-24, SG600-IL24 can significantly enhance the antitumor activity in HCC cell lines.