The Ni/SAPO-11 catalyst used for n-hexane hydroisomerization was prepared via a novel two-step crystallization strategy.It involves introduction of nickel salt into the pre-crystalized system of SAPO-11 by grinding fo...The Ni/SAPO-11 catalyst used for n-hexane hydroisomerization was prepared via a novel two-step crystallization strategy.It involves introduction of nickel salt into the pre-crystalized system of SAPO-11 by grinding followed by a second crystallization step.No extra solvent is introduced during the whole synthesis procedure which reduces waste liquid emissions significantly.More importantly,interaction between nickel and support is effectively regulated by the novel method to achieve a well dispersed nickel species and inhibit formation of inert nickel spinel simultaneously.Chemical environments of framework Si are tuned to enhance surface acidity of the Ni/SAPO-11 catalyst.It also shows smaller particle size which favors fast diffusion of reactants and products.Insights into the two-step crystallization indicate that accumulation of SAPO-11 precursors in the pre-crystallization stage,pH regulation by nickel salt and structural directing effect of Ni2+during the second crystallization period account for the rapid crystal growth and smaller particle size of the Ni/SAPO-11 catalyst.All the unique features endow the Ni/SAPO-11 catalyst higher activity and isomers selectivity than the Pt/SAPO-11 catalyst in n-hexane hydroisomerization.展开更多
Objective: To explore the mechanism of Radix Bupleuri Treatment of Breast Cancer through network pharmacology. Methods: TCMSP Database was used and related literature was collected to screen out the active constituent...Objective: To explore the mechanism of Radix Bupleuri Treatment of Breast Cancer through network pharmacology. Methods: TCMSP Database was used and related literature was collected to screen out the active constituents of Radix Bupleuri. Multiple databases were used to search the targets of the constituents and the disease. Next, a visual map of the compound-target-path network were constructed. The protein-protein interaction network was visually analyzed. Finally, the enrichment analysis of GO biological process and pathway enrichment analysis were carried out. Results: Active compounds of Radix Bupleuri related to disease targets have been obtained,and they play therapeutic roles for breast cancer through mainly regulating target proteins such as PTGS2, NOS2, AR and ESR. Meanwhile, the active compounds of Radix Bupleuri have an impact on biological processes such as steroid receptor activity and endocrine resistance, platinum resistance, breast cancer-related signaling pathways. Hence, it plays a role in the treatment of breast cancer. Conclusion: The results of this study preliminarily validate the target and mode of Radix Bupleuri in the treatment of Breast Cancer, and lay a foundation for further revealing its mechanism of action.展开更多
Objective:Human papillomavirus(HPV)causes condyloma acuminata,which is one of the most widespread sexually transmitted diseases.This study was aimed to evaluate the prevalence of HPV infection and HPV genotype distrib...Objective:Human papillomavirus(HPV)causes condyloma acuminata,which is one of the most widespread sexually transmitted diseases.This study was aimed to evaluate the prevalence of HPV infection and HPV genotype distribution in patients with condyloma acuminatum in Urumqi,China.Methods:A total of 835 cervical and genital samples were collected from outpatients treated for condyloma acuminatum between December 2016 and December 2018.HPV genotyping was performed with the HPV Genotyping Real-time PCR Kit for 23 different HPV types.Statistcal analysis was conducted using the chi-square test.Results:The total prevalence of HPV infection was 80.72%(674/835)in patients with condyloma acuminatum in Urumqi.The HPV genotypes were HPV-6(372/835,44.55%),HPV-11(180/835,21.56%),HPV-16(91/835,10.90%),HPV-43(65/835,7.78%),HPV-42(56/835,6.71%),and HPV-58(53/835,6.35%).Among HPV-infected individuals,49.11%(331/674)were infected by a single HPV subtype,while 50.89%(343/674)were infected by multiple HPV subtypes.The prevalence of HPV infection was higher in females(82.14%,276/336)than in males(79.76%,398/499)(χ^(2)=0.73,P=0.39),and higher in other ethnicities(86.36%,133/154)than in people of Han ethnicity(79.76%,541/681)(χ^(2)=3.866,P=0.049).The peak prevalence of HPV infection was observed in the age groups of 20-29 years and 30-39 years.Conclusion:HPV infection was detected in most patients with condyloma acuminatum in Urumqi with prevalence of 80.72%,and the most common HPV types were HPV-6,-11,-16,-43,-42,and-58.The detection of HPV genotypes is very important to monitor HPV infection so that effective treatment is administered to patients with condyloma acuminatum.展开更多
Background Previous studies investigated the association between gestational anemia and neonatal outcomes.However,few studies explored whether the effects of gestational anemia could be eliminated by subsequent correc...Background Previous studies investigated the association between gestational anemia and neonatal outcomes.However,few studies explored whether the effects of gestational anemia could be eliminated by subsequent correction of anemia in the later stages of pregnancy.This study aimed to investigate the relationship between anemia in different trimesters and neonatal outcomes.Methods The study was conducted in Shanghai,China,with a sample of 46,578 pregnant women who delivered between January 1,2016 and July 1,2019.A multivariable logistic regression model was adopted to analyse the associations between maternal anemia and neonatal outcomes.Results The incidence of gestational anemia was 30.2%,including 4.4%in the first trimester,9.6%in the second trimester,and 16.2%in the third trimester.Only 24.5%(507/2066)of anemia that occurred in the first trimester and 29.6%(1320/4457)that occurred in the second trimester could be corrected in the later stages of pregnancy.Anemia occurring in the first trimester was associated with small for gestational age[odds ratio(OR)1.46;95%confidence interval(CI)1.20-1.78]and with fetal distress(OR 1.23;95%CI 1.08-1.40).Anemia corrected in the first trimester also was associated with a higher risk of small for gestational age.Conclusions Gestational anemia is a public health problem in China impacting neonatal health.Anemia in pregnancy could be corrected in only about a quarter of the women.Anemia in the first trimester,whether corrected or not,still led to lower birth weight;therefore,the prevention of anemia prior to pregnancy is important.展开更多
We conducted a retrospective cohort study of 9,552 women experiencing their second delivery between 2014 and 2016 at the International Peace Maternity and Child Health Hospital to investigate the association between t...We conducted a retrospective cohort study of 9,552 women experiencing their second delivery between 2014 and 2016 at the International Peace Maternity and Child Health Hospital to investigate the association between the interpregnancy interval(IPI)and adverse perinatal outcomes. With the 12–23-mon IPI as the reference category, logistic regression analyzes were used to examine associations between different IPIs(<12, 12–23, 24–59, 60–119, and ≥120 mon) and perinatal outcomes(gestational diabetes mellitus, premature membrane rupture, gestational hypertension, preterm birth, low birth weight, and macrosomia).Compared with the 12–23-mon IPI category, women with longer IPIs had a higher risk of adverse perinatal outcomes, and those with an IPI ≥120 mon had the highest risk of gestational diabetes mellitus and premature membrane rupture(adjusted odds ratio(OR) 1.76, 95% confidence interval(CI) 1.32–2.35 and adjusted OR 2.03, 95% CI 1.53–2.67, respectively). These results indicate that a longer IPI is associated with a higher risk of adverse perinatal outcomes and an IPI of ≥120 mon appears to be independently associated with a higher risk of gestational diabetes mellitus and premature membrane rupture.展开更多
Basonuclin(BNC1)is expressed primarily in proliferative keratinocytes and gametogenic cells.However,its roles in spermatogenesis and testicular aging were not dear.Previously we discovered a heterozygous BNC1 truncati...Basonuclin(BNC1)is expressed primarily in proliferative keratinocytes and gametogenic cells.However,its roles in spermatogenesis and testicular aging were not dear.Previously we discovered a heterozygous BNC1 truncation mutation in a premature ovarian insufficiency pedigree.In this study,we found that male mice carrying the truncation mutation exhibited progressively fertility loss and testicular premature aging.Genome-wide expression profiling and direct binding studies(by chromatin immunoprecipitation sequencing)with BNC1 in mouse testis identified several spermatogenesis-specific gene promoters targeted by BNC1 including kelch-like family member 10(Klhl1O),testis expressed 14(Tex14)9 and spermatogenesis and centriole associated 1(Spatcl).Moreover,biochemical analysis showed that BNC1 was associated with TATA-box binding protein-associated factor 7 like(TAF7L),a germ cell-specific paralogue of the transcription factor IID subunit TAF7,both in vitro and in testis,suggesting that BNC1 might directly cooperate with TAF7L to regulate spermatogenesis.The truncation mutation disabled nuclear translocation of the BNC1/TAF7L complex,thus,disturbing expression of related genes and leading to testicular premature aging.Similarly,expressions of Y-box-binding protein 2(YBX2),outer dense fiber of sperm tails 1(ODfl),and glyceraldehyde-3-phosphate dehydrogenase,spermatogenic(GAPDHS)were significantly decreased in the testis of men with non-obstructive azoospermia.The present study adds to the understanding of the physiology of male reproductive aging and the mechanism of spermatogenic failure in infertile men.展开更多
Object: The study was aimed to detect the methylation patterns in psoriasis tissues. Methods: We collected 15 psoriasis tissues and 19 uninvolved psoriatic samples from psoriasis patients, and 3 normal skin samples fr...Object: The study was aimed to detect the methylation patterns in psoriasis tissues. Methods: We collected 15 psoriasis tissues and 19 uninvolved psoriatic samples from psoriasis patients, and 3 normal skin samples from healthy control, and investigated the methylation levels of p21 gene promoter by pyrosequencing analysis. The expression of p21 protein was detected by immunohischemistry in 38 psoriatic tissues and 16 uninvolved samples from psoriasis patients, and 3 normal samples from healthy control. The differences among the three groups were analyzed by Chi-square test. Results: We found that there was no significant difference in the frequency of p21 gene promoter methylation among the three groups (P=0.204), howerver the p21 protein expression were different among the three groups (P=0.007). Conclusion: This methylation marker maybe not an indicator for the detection and monitoring of psoriasis.展开更多
基金supported by the National Natural Science Foundation of China(22108308,21978326,21991091)the Natural Science Foundation of Shandong Province(ZR2021QB062,ZR2019MB029)+2 种基金the China Postdoctoral Science Foundation(2020M682259)the Project of Qingdao West Coast New Area District Science and Technology Innovation Center(Grant No.20202-2)the Fundamental Research Funds for the Central Universities(22CX06014A)。
文摘The Ni/SAPO-11 catalyst used for n-hexane hydroisomerization was prepared via a novel two-step crystallization strategy.It involves introduction of nickel salt into the pre-crystalized system of SAPO-11 by grinding followed by a second crystallization step.No extra solvent is introduced during the whole synthesis procedure which reduces waste liquid emissions significantly.More importantly,interaction between nickel and support is effectively regulated by the novel method to achieve a well dispersed nickel species and inhibit formation of inert nickel spinel simultaneously.Chemical environments of framework Si are tuned to enhance surface acidity of the Ni/SAPO-11 catalyst.It also shows smaller particle size which favors fast diffusion of reactants and products.Insights into the two-step crystallization indicate that accumulation of SAPO-11 precursors in the pre-crystallization stage,pH regulation by nickel salt and structural directing effect of Ni2+during the second crystallization period account for the rapid crystal growth and smaller particle size of the Ni/SAPO-11 catalyst.All the unique features endow the Ni/SAPO-11 catalyst higher activity and isomers selectivity than the Pt/SAPO-11 catalyst in n-hexane hydroisomerization.
基金National Natural Science Foundation of China(81970494)Fundamental Research Funds for the Central Universities of Central South University(502211903)
文摘Objective: To explore the mechanism of Radix Bupleuri Treatment of Breast Cancer through network pharmacology. Methods: TCMSP Database was used and related literature was collected to screen out the active constituents of Radix Bupleuri. Multiple databases were used to search the targets of the constituents and the disease. Next, a visual map of the compound-target-path network were constructed. The protein-protein interaction network was visually analyzed. Finally, the enrichment analysis of GO biological process and pathway enrichment analysis were carried out. Results: Active compounds of Radix Bupleuri related to disease targets have been obtained,and they play therapeutic roles for breast cancer through mainly regulating target proteins such as PTGS2, NOS2, AR and ESR. Meanwhile, the active compounds of Radix Bupleuri have an impact on biological processes such as steroid receptor activity and endocrine resistance, platinum resistance, breast cancer-related signaling pathways. Hence, it plays a role in the treatment of breast cancer. Conclusion: The results of this study preliminarily validate the target and mode of Radix Bupleuri in the treatment of Breast Cancer, and lay a foundation for further revealing its mechanism of action.
基金supported by the Technology Introduction and Innovation Projects of Peoples Hospital of Xinjiang Uygur Autonomous Region(No.20180304)。
文摘Objective:Human papillomavirus(HPV)causes condyloma acuminata,which is one of the most widespread sexually transmitted diseases.This study was aimed to evaluate the prevalence of HPV infection and HPV genotype distribution in patients with condyloma acuminatum in Urumqi,China.Methods:A total of 835 cervical and genital samples were collected from outpatients treated for condyloma acuminatum between December 2016 and December 2018.HPV genotyping was performed with the HPV Genotyping Real-time PCR Kit for 23 different HPV types.Statistcal analysis was conducted using the chi-square test.Results:The total prevalence of HPV infection was 80.72%(674/835)in patients with condyloma acuminatum in Urumqi.The HPV genotypes were HPV-6(372/835,44.55%),HPV-11(180/835,21.56%),HPV-16(91/835,10.90%),HPV-43(65/835,7.78%),HPV-42(56/835,6.71%),and HPV-58(53/835,6.35%).Among HPV-infected individuals,49.11%(331/674)were infected by a single HPV subtype,while 50.89%(343/674)were infected by multiple HPV subtypes.The prevalence of HPV infection was higher in females(82.14%,276/336)than in males(79.76%,398/499)(χ^(2)=0.73,P=0.39),and higher in other ethnicities(86.36%,133/154)than in people of Han ethnicity(79.76%,541/681)(χ^(2)=3.866,P=0.049).The peak prevalence of HPV infection was observed in the age groups of 20-29 years and 30-39 years.Conclusion:HPV infection was detected in most patients with condyloma acuminatum in Urumqi with prevalence of 80.72%,and the most common HPV types were HPV-6,-11,-16,-43,-42,and-58.The detection of HPV genotypes is very important to monitor HPV infection so that effective treatment is administered to patients with condyloma acuminatum.
基金This work was supported by the Special Fund for the National Key Research and Development Plan Grant(2017YFC1001300)the International Cooperation Project of China and Canada NSFC(81661128010)+2 种基金the Major Program of National Natural Science Foundation of China(81490742)the Natural Science Foundation of China(31571556)Innovative research team of high-level local universities in Shanghai,and Shanghai Municipal Key Clinical Speciality,Shanghai,China.
文摘Background Previous studies investigated the association between gestational anemia and neonatal outcomes.However,few studies explored whether the effects of gestational anemia could be eliminated by subsequent correction of anemia in the later stages of pregnancy.This study aimed to investigate the relationship between anemia in different trimesters and neonatal outcomes.Methods The study was conducted in Shanghai,China,with a sample of 46,578 pregnant women who delivered between January 1,2016 and July 1,2019.A multivariable logistic regression model was adopted to analyse the associations between maternal anemia and neonatal outcomes.Results The incidence of gestational anemia was 30.2%,including 4.4%in the first trimester,9.6%in the second trimester,and 16.2%in the third trimester.Only 24.5%(507/2066)of anemia that occurred in the first trimester and 29.6%(1320/4457)that occurred in the second trimester could be corrected in the later stages of pregnancy.Anemia occurring in the first trimester was associated with small for gestational age[odds ratio(OR)1.46;95%confidence interval(CI)1.20-1.78]and with fetal distress(OR 1.23;95%CI 1.08-1.40).Anemia corrected in the first trimester also was associated with a higher risk of small for gestational age.Conclusions Gestational anemia is a public health problem in China impacting neonatal health.Anemia in pregnancy could be corrected in only about a quarter of the women.Anemia in the first trimester,whether corrected or not,still led to lower birth weight;therefore,the prevention of anemia prior to pregnancy is important.
基金the Major Program of National Natural Science Foundation of China (81490742, 31471405, 81771593 and 81671456)the National Key Research and Development Program of China (2017YFC1001303 and 2018YFC1003200)+2 种基金the International Cooperation Project of China and Canada NSFC (81661128010)the Interdisciplinary Key Program of Shanghai Jiao Tong University (YG2014ZD08)the Shen Kang Three-Year Action Plan (16CR3003A)。
文摘We conducted a retrospective cohort study of 9,552 women experiencing their second delivery between 2014 and 2016 at the International Peace Maternity and Child Health Hospital to investigate the association between the interpregnancy interval(IPI)and adverse perinatal outcomes. With the 12–23-mon IPI as the reference category, logistic regression analyzes were used to examine associations between different IPIs(<12, 12–23, 24–59, 60–119, and ≥120 mon) and perinatal outcomes(gestational diabetes mellitus, premature membrane rupture, gestational hypertension, preterm birth, low birth weight, and macrosomia).Compared with the 12–23-mon IPI category, women with longer IPIs had a higher risk of adverse perinatal outcomes, and those with an IPI ≥120 mon had the highest risk of gestational diabetes mellitus and premature membrane rupture(adjusted odds ratio(OR) 1.76, 95% confidence interval(CI) 1.32–2.35 and adjusted OR 2.03, 95% CI 1.53–2.67, respectively). These results indicate that a longer IPI is associated with a higher risk of adverse perinatal outcomes and an IPI of ≥120 mon appears to be independently associated with a higher risk of gestational diabetes mellitus and premature membrane rupture.
基金This work was supported by the National Key Research and Development Program of China(2018YFC1005003,2017YFC1001003,and 2017YFC1001303)the National Natural Science Foundation of China(81471421,81401219,and 81701461)+3 种基金The Fundamental Research Funds for the Central Universities,Natural Science Foundation of Zhejiang Province(Q19H040040)Key Research Program of Zhejiang Provincial Natural Science Foundation(LZ18H040001)Municipal Human Resources Development Program for Outstanding Young Talents in Medical and Health Sciences in Shanghai(2018YQ39)Zhejiang University Education Foundation Global Partnership Fund.
文摘Basonuclin(BNC1)is expressed primarily in proliferative keratinocytes and gametogenic cells.However,its roles in spermatogenesis and testicular aging were not dear.Previously we discovered a heterozygous BNC1 truncation mutation in a premature ovarian insufficiency pedigree.In this study,we found that male mice carrying the truncation mutation exhibited progressively fertility loss and testicular premature aging.Genome-wide expression profiling and direct binding studies(by chromatin immunoprecipitation sequencing)with BNC1 in mouse testis identified several spermatogenesis-specific gene promoters targeted by BNC1 including kelch-like family member 10(Klhl1O),testis expressed 14(Tex14)9 and spermatogenesis and centriole associated 1(Spatcl).Moreover,biochemical analysis showed that BNC1 was associated with TATA-box binding protein-associated factor 7 like(TAF7L),a germ cell-specific paralogue of the transcription factor IID subunit TAF7,both in vitro and in testis,suggesting that BNC1 might directly cooperate with TAF7L to regulate spermatogenesis.The truncation mutation disabled nuclear translocation of the BNC1/TAF7L complex,thus,disturbing expression of related genes and leading to testicular premature aging.Similarly,expressions of Y-box-binding protein 2(YBX2),outer dense fiber of sperm tails 1(ODfl),and glyceraldehyde-3-phosphate dehydrogenase,spermatogenic(GAPDHS)were significantly decreased in the testis of men with non-obstructive azoospermia.The present study adds to the understanding of the physiology of male reproductive aging and the mechanism of spermatogenic failure in infertile men.
基金supported by the Hospital Project of People's Hospital of Xinjiang Uygur Autonomous Region(20190106).
文摘Object: The study was aimed to detect the methylation patterns in psoriasis tissues. Methods: We collected 15 psoriasis tissues and 19 uninvolved psoriatic samples from psoriasis patients, and 3 normal skin samples from healthy control, and investigated the methylation levels of p21 gene promoter by pyrosequencing analysis. The expression of p21 protein was detected by immunohischemistry in 38 psoriatic tissues and 16 uninvolved samples from psoriasis patients, and 3 normal samples from healthy control. The differences among the three groups were analyzed by Chi-square test. Results: We found that there was no significant difference in the frequency of p21 gene promoter methylation among the three groups (P=0.204), howerver the p21 protein expression were different among the three groups (P=0.007). Conclusion: This methylation marker maybe not an indicator for the detection and monitoring of psoriasis.