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Expression, purification and bioactivity of human augmenter of liver regeneration 被引量:2
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作者 Yang-De Zhang Jian Zhou +4 位作者 Jin-Feng Zhao Jian Peng Xiao-Dong liu xin-sheng liu Ze-Ming Jia 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第27期4401-4405,共5页
瞄准:为肝新生(hALR ) 的原核生物、真核细胞的人的 augmenter 构造表示向量并且学习他们的生物活动。方法:hALRcDNA 克隆从原生质标志 pGEM-T-hALR 被获得,并且 cDNA 是克隆进 prokatyotic 表示向量 pGEX-4T-2 的潜水艇。recombina... 瞄准:为肝新生(hALR ) 的原核生物、真核细胞的人的 augmenter 构造表示向量并且学习他们的生物活动。方法:hALRcDNA 克隆从原生质标志 pGEM-T-hALR 被获得,并且 cDNA 是克隆进 prokatyotic 表示向量 pGEX-4T-2 的潜水艇。recombinant 向量和 pGEX-4T-2hALR 被酶消化并且 DNA 定序识别并且转变了成 E 关口 i JM109。断然选择的克隆被 GST-hALR 熔化蛋白质的表示与 IPTG 导致,然后,熔化蛋白质被 glutathine s-transferase (GST ) 净化 sepharose 4B 亲密关系层析,由测量 H 胸腺嘧啶核甙加入由凝血酵素劈开, hALR 单体被获得并且检测。结果:从原生质标志 pGEM-T-hALR 的 PCR 的产品被 1.5% sepharose 电气泳动检验。特定的带与理论的是重合的。顺序是精确的,酶消化的 pGEX-4T-hALP 与理论的是重合的。顺序是精确的,碎片在积极方向被插入。recombinant 向量被转变成 E 关口 i JM109。SDS 页证明导致的富有表达力的熔化蛋白质与 41 kDa 的分子量给一个单身的乐队看了。产品被净化并且劈开。GST 和 hALR 的分子量是 26 kDa, 15 kDa 分别地。recombinant 熔化蛋白质说明了细菌的溶解产物的 31% 全部的可溶的蛋白质。在主要文化和 HepG2 房间线加到成年老鼠 hepatocytes 的培养基的 HALR 能显著地与相关控制组相比提高 DNA 合成的率(P 【 0.01 ) 。结论:净化的 hALR 有能力刺激 DNA 在主要文化和 HepG2 房间在试管内的成年老鼠 hepatocytes 的合成,和罐头为它的临床的申请提供证据。 展开更多
关键词 净化方法 基因表达 生物活性 肝再生
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An Upper Bound for the Adjacent Vertex Distinguishing Acyclic Edge Chromatic Number of a Graph 被引量:15
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作者 xin-sheng liu Ming-qiang An Yang Gao 《Acta Mathematicae Applicatae Sinica》 SCIE CSCD 2009年第1期137-140,共4页
A proper k-edge coloring of a graph G is called adjacent vertex distinguishing acyclic edge coloring if there is no 2-colored cycle in G and the color set of edges incident to u is not equal to the color set of edges ... A proper k-edge coloring of a graph G is called adjacent vertex distinguishing acyclic edge coloring if there is no 2-colored cycle in G and the color set of edges incident to u is not equal to the color set of edges incident to v, where uv ∈E(G). The adjacent vertex distinguishing acyclic edge chromatic number of G, denoted by χ'αα(G), is the minimal number of colors in an adjacent vertex distinguishing acyclic edge coloring of G. In this paper we prove that if G(V, E) is a graph with no isolated edges, then χ'αα(G)≤32△. 展开更多
关键词 Adjacent strong edge coloring adjacent vertex distinguishing acyclic edge coloring adjacent vertexdistinguishing acyclic edge chromatic number the LovNsz local lemma
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Mortality Trend and Predictors of Mortality in Dysphagic Stroke Patients Postpercutaneous Endoscopic Gastrostomy
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作者 Yue-Long Jiang Nyoka Ruberu +3 位作者 xin-sheng liu Ying-Hua Xu Shu-Tian Zhang Daniel KY Chan 《Chinese Medical Journal》 SCIE CAS CSCD 2015年第10期1331-1335,共5页
Background:Percutaneous endoscopic gastrostomy (PEG) feeding is widely used in stroke patients suffering from persistent dysphagia;however,predicting the risks and benefits of PEG insertion in the individual patien... Background:Percutaneous endoscopic gastrostomy (PEG) feeding is widely used in stroke patients suffering from persistent dysphagia;however,predicting the risks and benefits of PEG insertion in the individual patient is difficult.The aim of our study was to investigate if candidate risk factors could predict short-term mortality risk in poststroke patients who had PEG tube insertion for persistent dysphagia.Methods:This was a retrospective study of 3504 consecutive stroke patients admitted to two metropolitan hospitals during the period January 2005 to December 2013 and who also underwent PEG insertion for feeding due to persistent dysphagia.Results:A total of 102 patients were included in the study.There were 22 deaths in 6 months after insertion of PEG tubes and 20 deaths of those occurred within 3 months post PEG.Those who survived beyond 6 months showed significantly lower mean age (75.9 ± 9.0 years vs.83.0 ± 4.9 years,P 〈 0.001),a lower mean American Society of Anesthesia (ASA) score (3.04 ± 0.63 vs.3.64 ± 0.58,P 〈 0.001) compared to nonsurvivors.In multiple Logistic,age (P =0.004,odds ratio [OR] =1.144;95% confidence interval [CI]:1.044-1.255);ASA (P =0.002,OR =5.065;95% CI:1.815-14.133) and albumin level pre-PEG insertion (P =0.033,OR =0.869;95% CI:0.764-0.988)were the independent determinants of mortality respectively.Conclusions:We propose that age,ASA score and albumin level pre-PEG insertion to be included as factors to assist in the selection of patients who are likely to survive more than 3 months post PEG insertion. 展开更多
关键词 Age ALBUMIN American Society of Anesthesia MORTALITY Percutaneous Endoscopic Gastrostomy STROKE
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