Bibliometric analysis of subject trends and knowledge structures of gut microbiota

BACKGROUND Gut microbiota is an emerging field of research,with related research having breakthrough development in the past 15 years.Bibliometric analysis can be applied to analyze the evolutionary trends and emerging hotspots in this field.AIM To study the subject trends and knowledge structures of gut microbiota related research fields from 2004 to 2018.METHODS The literature data on gut microbiota were identified and downloaded from the PubMed database.Through biclustering analysis,strategic diagrams,and social network analysis diagrams,the main trend and knowledge structure of research fields concerning gut microbiota were analyzed to obtain and compare the research hotspots in each period.RESULTS According to the strategic coordinates and social relationship network map,Clostridium Infections/microbiology,Clostridium Infections/therapy,RNA,Ribosomal,16S/genetics,Microbiota/genetics,Microbiota/immunology,Dysbiosis/immunology,Infla-mmation/immunology,Fecal Microbiota Transplantation/methods,Fecal Microbiota Transplantation can be used as an emerging research hotspot in the past 5 years(2014-2018).CONCLUSION Some subjects were not yet fully studied according to the strategic coordinates;and the emerging hotspots in the social network map can be considered as directions of future research.

Brusatol Inhibits Proliferation,Migration,and Invasion of Nonsmall Cell Lung Cancer PC-9 Cells

Objective:The purpose of this study was to investigate the inhibitory effect of brusatol,a nigakilactone extracted from Brucea javanica,on lung cancer for development of therapeutic drugs.We explored the effects of brusatol on the proliferation,migration,and invasion of lung cancer PC-9 cells in vitro and analyzed the mechanisms involved.Materials and Methods:MTT assay was used to determine the effect of brusatol on the proliferative capacity of PC-9 and H1975 cells in vitro.The half-maximal inhibitory concentrations(IC_(50))were calculated and used as a reference for subsequent experiments.Variations in the number and size of tumor cell clusters were monitored by the colony formation assay as evidence for the effect of brusatol on cell proliferation.The effect of brusatol on the migration and invasion of PC-9 cells was evaluated using wound healing and transwell assays,respectively.Apoptosis in lung cancer cells was detected using the Annexin V-FITC/propidium iodide assay.The correlated anticancer mechanism was detected using Western blotting.Results:The IC_(50)values of brusatol acting on PC-9 and H1975 cells were 1.58±0.30μM and 31.34±2.72μM,respectively,according to the MTT experiment.In addition,brusatol suppressed PC-9 cell proliferation,migration,and invasion,as well as induced apoptosis,which may be related to the downregulation of epidermal growth factor receptor(EGFR),β-catenin,Akt,and STAT3 expression.Conclusions:Brusatol showed potent anticancer activity against lung cancer PC-9 cells,inhibiting the proliferative capacity and metastatic potential of PC-9 cells.Its anticancer effect may be related to the downregulation of EGFR,β-catenin,Akt,and STAT3.