Background:Early diagnosis and classification of infections increase the cure rate while decreasing complications,which is significant for severe infections,especially for war surgery.However,traditional methods rely ...Background:Early diagnosis and classification of infections increase the cure rate while decreasing complications,which is significant for severe infections,especially for war surgery.However,traditional methods rely on laborious operations and bulky devices.On the other hand,point-of-care(POC)methods suffer from limited robustness and accuracy.Therefore,it is of urgent demand to develop POC devices for rapid and accurate diagnosis of infections to fulfill on-site militarized requirements.Methods:We developed a wave-shaped microfluidic chip(WMC)assisted multiplexed detection platform(WMC-MDP).WMC-MDP reduces detection time and improves repeatability through premixing of the samples and reaction of the reagents.We further combined the detection platform with the streptavidin–biotin(SA-B)amplified system to enhance the sensitivity while using chemiluminescence(CL)intensity as signal readout.We realized simultaneous detection of C-reactive protein(CRP),procalcitonin(PCT),and interleukin-6(IL-6)on the detection platform and evaluated the sensitivity,linear range,selectivity,and repeatability.Finally,we finished detecting 15 samples from volunteers and compared the results with commercial ELISA kits.Results:Detection of CRP,PCT,and IL-6 exhibited good linear relationships between CL intensities and concentrations in the range of 1.25–40μg/ml,0.4–12.8 ng/ml,and 50–1600 pg/ml,respectively.The limit of detection of CRP,PCT,and IL-6 were 0.54μg/ml,0.11 ng/ml,and 16.25 pg/ml,respectively.WMC-MDP is capable of good adequate selectivity and repeatability.The whole detection procedure takes only 22 min that meets the requirements of a POC device.Results of 15 samples from volunteers were consistent with the results detected by commercial ELISA kits.Conclusions:WMC-MDP allows simultaneous,rapid,and sensitive detection of CRP,PCT,and IL-6 with satisfactory selectivity and repeatability,requiring minimal manipulation.However,WMC-MDP takes advantage of being a microfluidic device showing the coefficients of variation less than 10%enabling WMC-MDP to be a type of point-of-care testing(POCT).Therefore,WMC-MDP provides a promising alternative to POCT of multiple biomarkers.We believe the practical application of WMC-MDP in militarized fields will revolutionize infection diagnosis for soldiers.展开更多
The first TEMPO-mediated 'living' free radical polymerization of liquid crystalline monomer, 2, 5-bis[(4-methoxyphenyl)oxycarbonyl]styrene (MPCS), was carried out at 130 degrees C with BPO as an initiator. The...The first TEMPO-mediated 'living' free radical polymerization of liquid crystalline monomer, 2, 5-bis[(4-methoxyphenyl)oxycarbonyl]styrene (MPCS), was carried out at 130 degrees C with BPO as an initiator. The molecular weight of the polymer can be varied from rather low values to high values while maintaining narrow polydispersity. It was observed that the polymerization of MPCS proceeded much faster than that of styrene. A tentative explanation for this fast polymerization was suggested.展开更多
To allow anisotropies of optical properties in a magnetic field, nitroxide radical is introduced into the ortho-position of the phenylene ring in the side chain. A new azobenzene side-chain polymer (TEMPO-PAZ) contain...To allow anisotropies of optical properties in a magnetic field, nitroxide radical is introduced into the ortho-position of the phenylene ring in the side chain. A new azobenzene side-chain polymer (TEMPO-PAZ) containing TEMPO radical was synthesized. The polymer has a good solubility in organic solvents. The ESR spectrum of the polymer indicated three absorption lines characteristic of TEMPO radical. The optical phase conjugated responses (I-4) of the polymer films were investigated by degenerate four-wave mixing (DFWM). The experimental results showed that optical phase conjugated response of the TEMPO-PAZ could be easily controlled by choosing the appropriate direction of magnetic field presumably due to the nitroxide radical in the TEMPO-PAZ molecular structure. For the polymer investigated here, the nitroxide radical was introduced to increase optical phase conjugated response intensity in a magnetic field, aiming originally at searching for a new photo-active organic magnetic multifunctional materials.展开更多
The photopolymerization of two kinds of prepolyesters containing conjugated diacetylene (PDA-6 and PDA-12) was investigated using DSC. By measuring the endothermic enthalpy of the prepolymers after different UV-irradi...The photopolymerization of two kinds of prepolyesters containing conjugated diacetylene (PDA-6 and PDA-12) was investigated using DSC. By measuring the endothermic enthalpy of the prepolymers after different UV-irradiation times, the polymerizations were found to follow the first-order rate law which agreed with the results of other investigators using a different method. The endothermic enthalpy measurements of PDA-6 and PDA-12 before UV-irradiation precluded the possibility that the decrease of endothermic enthalpy was caused by thermal polymerization.展开更多
Novel side-chain liquid-crystalline poly(meth)acrylates were synthesized using 1-(3-hydroxyl-propyl)-3-[(4 '-cyano-p-terphenyloxycarbonyl)alkyl]-1,1,3,3-tetramethyldisiloxane as the key intermediate. The polymers ...Novel side-chain liquid-crystalline poly(meth)acrylates were synthesized using 1-(3-hydroxyl-propyl)-3-[(4 '-cyano-p-terphenyloxycarbonyl)alkyl]-1,1,3,3-tetramethyldisiloxane as the key intermediate. The polymers used a disiloxane moiety as decoupling spacer with cyano-p-terphenyl as mesogenic unit. The products were characterized by NMR, GPC, DSC and polarizing optical microscopy. All the polymers with cyano-p-terphenyl mesogens formed a stable mesophase. However, if the mesogenic unit is replaced by cyano-p-biphenyl, the liquid crystalline character will be lost. The results also showed that the decoupling is incomplete even if a complex and very flexible decoupling spacer is deliberately incorporated to obtain the highest possible decoupling effect.展开更多
Some diacetylene-containing copolyesters with different ratios of dipropargyl 1,10-decanate anddipropargyl terephthalate were synthesized. Properties of these copolymers were characterized by differentialscanning calo...Some diacetylene-containing copolyesters with different ratios of dipropargyl 1,10-decanate anddipropargyl terephthalate were synthesized. Properties of these copolymers were characterized by differentialscanning calorimetry and polarizing optical microscopy. The copolymer could be cross-linked easily by UVirradiation in a liquid crystal matrix without adding any photoinitiator. The preliminary studies showed thatthe diacetylene-containing polymer network can assist liquid crystal molecules to align with the externalelectric field, thus reducing the response time of display devices.展开更多
A variety of mesomorphic side-chain polysiloxanes were synthesized by using hydrosilylation reaction with dicyclopentadienylplatinum(II) (DPPC) as the catalyst. The mesogenic groups are derivatives of stilbene with cy...A variety of mesomorphic side-chain polysiloxanes were synthesized by using hydrosilylation reaction with dicyclopentadienylplatinum(II) (DPPC) as the catalyst. The mesogenic groups are derivatives of stilbene with cyano or nitro tail groups. The products were characterized by DSC, X-ray diffraction and polarizing optical microscopy (POM). All the polymers were found to have relatively low T-g and can form a smectic liquid crystal phase.展开更多
A novel switchable luminescent thermometer based on thermo-responsive triblock copolymer poly(ethylene glycol)-bpoly(acrylamide-co-acrylonitrile-co-dimethylaminoethylmethacrylate)(PEO113-b-P(AAm264-co-AN112-co-DMA8)) ...A novel switchable luminescent thermometer based on thermo-responsive triblock copolymer poly(ethylene glycol)-bpoly(acrylamide-co-acrylonitrile-co-dimethylaminoethylmethacrylate)(PEO113-b-P(AAm264-co-AN112-co-DMA8)) and Eu-containing polyoxometalate (Eu-POM) was successfully constructed.The copolymer synthesized by RAFT exhibited a linear response to temperature variations in aqueous media,which was quite different from the uncharged copolymer P(AAm-co-AN) having a specific upper critical solution temperature (UCST).Eu-POM was surrounded around thermo-responsive blocks through electrostatic interactions,and its luminescence could be finely tuned due to the sensitivity of copolymer to the temperature variation.In cold water,POMs were trapped in highly hydrophobic cores,exhibiting an intense emission.With the upraising of temperature,the emission intensity presented a gradual decrease and showed a linear correlation with temperature.When the complex solution cooled down,the luminescence could also be perfectly restored.This temperature-luminescence correlation could be held for numerous trials,showing a potential application in thermometer.展开更多
A series of novel cis poly(phenylacetylene)s(PPAs)substituted at meta-position(s)by two alkoxycarbonyl pendants,Le.,sP-Me-C8/rP-Me-C8,P-Me-C12,sP-Et-C4,sP-2C4 and sP-Oct-C4,were synthesized under the catalysis of[Rh(n...A series of novel cis poly(phenylacetylene)s(PPAs)substituted at meta-position(s)by two alkoxycarbonyl pendants,Le.,sP-Me-C8/rP-Me-C8,P-Me-C12,sP-Et-C4,sP-2C4 and sP-Oct-C4,were synthesized under the catalysis of[Rh(nbd)Cl]2(nbd=norbornadiene).The dependence of elongation,screw sense,and stimuli response of helical polyene backbone on the structure of pendant,solvent,and temperature was systematically investigated in both solution and solid states.Because of n→π*interaction between vicinal carbonyl groups,sP-Me-C8/rP-Me-C8 could adopt contracted cis-cisoid helix in THF,toluene,CH2Cl2,and CHCl3.Such an intramolecular interaction was sensitive to the hydrogen bond donating ability of solvent and temperature,but insensitive to the dielectric constant and polarity of solvent.In poly(3-methoxycarbonyl-5-alkoxycarbonylphenylacetylene),the longer the chiral alkyl chain was,the easier the stable cis-cisoid helix could be achieved.However,when the methoxycarbonyl was changed to ethoxycarbonyl,sec-butyloxycarbonyl,and octyloxycarbonyl pendant groups,only cistransoid helix was obtained at room temperature due to the increased steric hindrance.Moreover,lowering temperature was found to facilitate the stabilization of n→π*interactions,and reversible temperature-dependent stereomutations were achieved in sP-Me-C8 and sP-Et-C4depending on the solvent where they were dissolved.These results suggested that the long alkyl chain,small pendant size,and lower temperature favored the stabilization of intramolecular n→π*interactions and the formation of contracted,cis-cisoid helices for poly(3,5-diestersubstituted phenylacetylene)s.展开更多
基金the National Natural Science Foundation of China(81902167,52075138)the Natural Science Foundation of Jiangsu Province(BK20190872).
文摘Background:Early diagnosis and classification of infections increase the cure rate while decreasing complications,which is significant for severe infections,especially for war surgery.However,traditional methods rely on laborious operations and bulky devices.On the other hand,point-of-care(POC)methods suffer from limited robustness and accuracy.Therefore,it is of urgent demand to develop POC devices for rapid and accurate diagnosis of infections to fulfill on-site militarized requirements.Methods:We developed a wave-shaped microfluidic chip(WMC)assisted multiplexed detection platform(WMC-MDP).WMC-MDP reduces detection time and improves repeatability through premixing of the samples and reaction of the reagents.We further combined the detection platform with the streptavidin–biotin(SA-B)amplified system to enhance the sensitivity while using chemiluminescence(CL)intensity as signal readout.We realized simultaneous detection of C-reactive protein(CRP),procalcitonin(PCT),and interleukin-6(IL-6)on the detection platform and evaluated the sensitivity,linear range,selectivity,and repeatability.Finally,we finished detecting 15 samples from volunteers and compared the results with commercial ELISA kits.Results:Detection of CRP,PCT,and IL-6 exhibited good linear relationships between CL intensities and concentrations in the range of 1.25–40μg/ml,0.4–12.8 ng/ml,and 50–1600 pg/ml,respectively.The limit of detection of CRP,PCT,and IL-6 were 0.54μg/ml,0.11 ng/ml,and 16.25 pg/ml,respectively.WMC-MDP is capable of good adequate selectivity and repeatability.The whole detection procedure takes only 22 min that meets the requirements of a POC device.Results of 15 samples from volunteers were consistent with the results detected by commercial ELISA kits.Conclusions:WMC-MDP allows simultaneous,rapid,and sensitive detection of CRP,PCT,and IL-6 with satisfactory selectivity and repeatability,requiring minimal manipulation.However,WMC-MDP takes advantage of being a microfluidic device showing the coefficients of variation less than 10%enabling WMC-MDP to be a type of point-of-care testing(POCT).Therefore,WMC-MDP provides a promising alternative to POCT of multiple biomarkers.We believe the practical application of WMC-MDP in militarized fields will revolutionize infection diagnosis for soldiers.
基金This work was supported by the Foundation of Peking University for Young Scientist and the National Natural Science Foundation of China(Grant No.29874003).
文摘The first TEMPO-mediated 'living' free radical polymerization of liquid crystalline monomer, 2, 5-bis[(4-methoxyphenyl)oxycarbonyl]styrene (MPCS), was carried out at 130 degrees C with BPO as an initiator. The molecular weight of the polymer can be varied from rather low values to high values while maintaining narrow polydispersity. It was observed that the polymerization of MPCS proceeded much faster than that of styrene. A tentative explanation for this fast polymerization was suggested.
基金This work was supported by National Natural Science Foundation of China (No. 20134010) and Scientific Foundation for Returned Overseas Chinese Scholars. Ministry of Education.
文摘To allow anisotropies of optical properties in a magnetic field, nitroxide radical is introduced into the ortho-position of the phenylene ring in the side chain. A new azobenzene side-chain polymer (TEMPO-PAZ) containing TEMPO radical was synthesized. The polymer has a good solubility in organic solvents. The ESR spectrum of the polymer indicated three absorption lines characteristic of TEMPO radical. The optical phase conjugated responses (I-4) of the polymer films were investigated by degenerate four-wave mixing (DFWM). The experimental results showed that optical phase conjugated response of the TEMPO-PAZ could be easily controlled by choosing the appropriate direction of magnetic field presumably due to the nitroxide radical in the TEMPO-PAZ molecular structure. For the polymer investigated here, the nitroxide radical was introduced to increase optical phase conjugated response intensity in a magnetic field, aiming originally at searching for a new photo-active organic magnetic multifunctional materials.
文摘The photopolymerization of two kinds of prepolyesters containing conjugated diacetylene (PDA-6 and PDA-12) was investigated using DSC. By measuring the endothermic enthalpy of the prepolymers after different UV-irradiation times, the polymerizations were found to follow the first-order rate law which agreed with the results of other investigators using a different method. The endothermic enthalpy measurements of PDA-6 and PDA-12 before UV-irradiation precluded the possibility that the decrease of endothermic enthalpy was caused by thermal polymerization.
基金This work was supported by NNSFC (No. 59873001).
文摘Novel side-chain liquid-crystalline poly(meth)acrylates were synthesized using 1-(3-hydroxyl-propyl)-3-[(4 '-cyano-p-terphenyloxycarbonyl)alkyl]-1,1,3,3-tetramethyldisiloxane as the key intermediate. The polymers used a disiloxane moiety as decoupling spacer with cyano-p-terphenyl as mesogenic unit. The products were characterized by NMR, GPC, DSC and polarizing optical microscopy. All the polymers with cyano-p-terphenyl mesogens formed a stable mesophase. However, if the mesogenic unit is replaced by cyano-p-biphenyl, the liquid crystalline character will be lost. The results also showed that the decoupling is incomplete even if a complex and very flexible decoupling spacer is deliberately incorporated to obtain the highest possible decoupling effect.
基金This work was supported by the National Natural Science Foundation of China
文摘Some diacetylene-containing copolyesters with different ratios of dipropargyl 1,10-decanate anddipropargyl terephthalate were synthesized. Properties of these copolymers were characterized by differentialscanning calorimetry and polarizing optical microscopy. The copolymer could be cross-linked easily by UVirradiation in a liquid crystal matrix without adding any photoinitiator. The preliminary studies showed thatthe diacetylene-containing polymer network can assist liquid crystal molecules to align with the externalelectric field, thus reducing the response time of display devices.
基金This work was supported by the National Natural Science Foundation of China.
文摘A variety of mesomorphic side-chain polysiloxanes were synthesized by using hydrosilylation reaction with dicyclopentadienylplatinum(II) (DPPC) as the catalyst. The mesogenic groups are derivatives of stilbene with cyano or nitro tail groups. The products were characterized by DSC, X-ray diffraction and polarizing optical microscopy (POM). All the polymers were found to have relatively low T-g and can form a smectic liquid crystal phase.
基金financially supported by the National Natural Science Foundation of China (Nos. 51673002, 51873001, and 51833001)
文摘A novel switchable luminescent thermometer based on thermo-responsive triblock copolymer poly(ethylene glycol)-bpoly(acrylamide-co-acrylonitrile-co-dimethylaminoethylmethacrylate)(PEO113-b-P(AAm264-co-AN112-co-DMA8)) and Eu-containing polyoxometalate (Eu-POM) was successfully constructed.The copolymer synthesized by RAFT exhibited a linear response to temperature variations in aqueous media,which was quite different from the uncharged copolymer P(AAm-co-AN) having a specific upper critical solution temperature (UCST).Eu-POM was surrounded around thermo-responsive blocks through electrostatic interactions,and its luminescence could be finely tuned due to the sensitivity of copolymer to the temperature variation.In cold water,POMs were trapped in highly hydrophobic cores,exhibiting an intense emission.With the upraising of temperature,the emission intensity presented a gradual decrease and showed a linear correlation with temperature.When the complex solution cooled down,the luminescence could also be perfectly restored.This temperature-luminescence correlation could be held for numerous trials,showing a potential application in thermometer.
基金This work was financially supported by the National Natural Science Foundation of China(Nos.51833001 and 21674002).
文摘A series of novel cis poly(phenylacetylene)s(PPAs)substituted at meta-position(s)by two alkoxycarbonyl pendants,Le.,sP-Me-C8/rP-Me-C8,P-Me-C12,sP-Et-C4,sP-2C4 and sP-Oct-C4,were synthesized under the catalysis of[Rh(nbd)Cl]2(nbd=norbornadiene).The dependence of elongation,screw sense,and stimuli response of helical polyene backbone on the structure of pendant,solvent,and temperature was systematically investigated in both solution and solid states.Because of n→π*interaction between vicinal carbonyl groups,sP-Me-C8/rP-Me-C8 could adopt contracted cis-cisoid helix in THF,toluene,CH2Cl2,and CHCl3.Such an intramolecular interaction was sensitive to the hydrogen bond donating ability of solvent and temperature,but insensitive to the dielectric constant and polarity of solvent.In poly(3-methoxycarbonyl-5-alkoxycarbonylphenylacetylene),the longer the chiral alkyl chain was,the easier the stable cis-cisoid helix could be achieved.However,when the methoxycarbonyl was changed to ethoxycarbonyl,sec-butyloxycarbonyl,and octyloxycarbonyl pendant groups,only cistransoid helix was obtained at room temperature due to the increased steric hindrance.Moreover,lowering temperature was found to facilitate the stabilization of n→π*interactions,and reversible temperature-dependent stereomutations were achieved in sP-Me-C8 and sP-Et-C4depending on the solvent where they were dissolved.These results suggested that the long alkyl chain,small pendant size,and lower temperature favored the stabilization of intramolecular n→π*interactions and the formation of contracted,cis-cisoid helices for poly(3,5-diestersubstituted phenylacetylene)s.
