Small RNAs(sRNAs)are key players in the regulation of bacterial gene expression.However,the distribution and regulatory functions of sRNA in pig farm wastewater treatment plants(WWTPs)remains unknown.In this study,the...Small RNAs(sRNAs)are key players in the regulation of bacterial gene expression.However,the distribution and regulatory functions of sRNA in pig farm wastewater treatment plants(WWTPs)remains unknown.In this study,the wastewaters in anoxic and oxic tanks of the WWTPs were collected.The profiles of the community structure,mRNA expression,and sRNA expression of bacteria in pig farm wastewater were investigated using transcriptome sequencing and qPCR.This study demonstrated that there was a higher abundance of sRNA in the pig farm WWTPs and 52 sRNAs were detected.The sRNAs were mainly present in Proteobacteria and Firmicutes,including the potential human pathogenic bacteria(HPB)(Escherichia,Shigella,Bordetella and Morganella),crop pathogen(Pectobacterium)and denitrifying bacteria(Zobellella).And the sRNAs were involved in the bacterial functional activities such as translation,transcription,drug resistance,membrane transport and amino acid metabolism.In addition,most sRNAs had a higher abundance in anoxic tanks which contained a higher abundance of the genes associated with infectious diseases and drug resistance than that in oxic tanks.The results presented here show that in pig farm WWTPs,sRNA played an important role in bacterial function activities,especially the infectious diseases,drug resistance and denitrification,which can provide a new point of penetration for improving the pig farm WWTPs.展开更多
Effects of antibiotic residues on methane production in anaerobic digestion are commonly studied using the following two antibiotic addition methods:(1) adding manure from animals that consume a diet containing ant...Effects of antibiotic residues on methane production in anaerobic digestion are commonly studied using the following two antibiotic addition methods:(1) adding manure from animals that consume a diet containing antibiotics, and(2) adding antibiotic-free animal manure spiked with antibiotics. This study used chlortetracycline(CTC) as a model antibiotic to examine the effects of the antibiotic addition method on methane production in anaerobic digestion under two different swine wastewater concentrations(0.55 and 0.22 mg CTC/g dry manure). The results showed that CTC degradation rate in which manure was directly added at 0.55 mg CTC/g(HSPIKE treatment) was lower than the control values and the rest of the treatment groups. Methane production from the HSPIKEtreatment was reduced(p 〈 0.05) by 12% during the whole experimental period and 15% during the first 7 days. The treatments had no significant effect on the pH and chemical oxygen demand value of the digesters, and the total nitrogen of the0.55 mg CTC/kg manure collected from mediated swine was significantly higher than the other values. Therefore, different methane production under different antibiotic addition methods might be explained by the microbial activity and the concentrations of antibiotic intermediate products and metabolites. Because the primary entry route of veterinary antibiotics into an anaerobic digester is by contaminated animal manure, the most appropriate method for studying antibiotic residue effects on methane production may be using manure from animals that are given a particular antibiotic, rather than adding the antibiotic directly to the anaerobic digester.展开更多
基金financially supported by the Guangdong Technological Innovation Strategy of Special Funds(Key Areas of Research and Development Program,No.2018B020205003)
文摘Small RNAs(sRNAs)are key players in the regulation of bacterial gene expression.However,the distribution and regulatory functions of sRNA in pig farm wastewater treatment plants(WWTPs)remains unknown.In this study,the wastewaters in anoxic and oxic tanks of the WWTPs were collected.The profiles of the community structure,mRNA expression,and sRNA expression of bacteria in pig farm wastewater were investigated using transcriptome sequencing and qPCR.This study demonstrated that there was a higher abundance of sRNA in the pig farm WWTPs and 52 sRNAs were detected.The sRNAs were mainly present in Proteobacteria and Firmicutes,including the potential human pathogenic bacteria(HPB)(Escherichia,Shigella,Bordetella and Morganella),crop pathogen(Pectobacterium)and denitrifying bacteria(Zobellella).And the sRNAs were involved in the bacterial functional activities such as translation,transcription,drug resistance,membrane transport and amino acid metabolism.In addition,most sRNAs had a higher abundance in anoxic tanks which contained a higher abundance of the genes associated with infectious diseases and drug resistance than that in oxic tanks.The results presented here show that in pig farm WWTPs,sRNA played an important role in bacterial function activities,especially the infectious diseases,drug resistance and denitrification,which can provide a new point of penetration for improving the pig farm WWTPs.
基金supported by the National Natural Science Foundation of China (Nos. 30700586, 31072067, 31272481)the National Spark Program Project (No. 2012GA780001)the China Scholarship Council (NO. 201308440210)
文摘Effects of antibiotic residues on methane production in anaerobic digestion are commonly studied using the following two antibiotic addition methods:(1) adding manure from animals that consume a diet containing antibiotics, and(2) adding antibiotic-free animal manure spiked with antibiotics. This study used chlortetracycline(CTC) as a model antibiotic to examine the effects of the antibiotic addition method on methane production in anaerobic digestion under two different swine wastewater concentrations(0.55 and 0.22 mg CTC/g dry manure). The results showed that CTC degradation rate in which manure was directly added at 0.55 mg CTC/g(HSPIKE treatment) was lower than the control values and the rest of the treatment groups. Methane production from the HSPIKEtreatment was reduced(p 〈 0.05) by 12% during the whole experimental period and 15% during the first 7 days. The treatments had no significant effect on the pH and chemical oxygen demand value of the digesters, and the total nitrogen of the0.55 mg CTC/kg manure collected from mediated swine was significantly higher than the other values. Therefore, different methane production under different antibiotic addition methods might be explained by the microbial activity and the concentrations of antibiotic intermediate products and metabolites. Because the primary entry route of veterinary antibiotics into an anaerobic digester is by contaminated animal manure, the most appropriate method for studying antibiotic residue effects on methane production may be using manure from animals that are given a particular antibiotic, rather than adding the antibiotic directly to the anaerobic digester.