为了获得鸡热休克蛋白70(heat shock protein 70,HSP70)基因启动子区CpG岛的甲基化状态,初步探索HSP70基因在肌肉组织生长发育中的作用,试验以文昌鸡和北京油鸡为试验材料,采用MassArray质谱法检测鸡胸肌中HSP70基因启动子区CpG岛的甲...为了获得鸡热休克蛋白70(heat shock protein 70,HSP70)基因启动子区CpG岛的甲基化状态,初步探索HSP70基因在肌肉组织生长发育中的作用,试验以文昌鸡和北京油鸡为试验材料,采用MassArray质谱法检测鸡胸肌中HSP70基因启动子区CpG岛的甲基化水平,比较分析文昌鸡和北京油鸡HSP70基因的甲基化差异。结果表明:在HSP70基因启动子中共检测到65个CpG位点的甲基化水平;文昌鸡胸肌生长发育过程中有8个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈下降趋势;北京油鸡胸肌生长发育过程中只有3个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈升高趋势,第120天时甲基化水平达到最高。说明文昌鸡与北京油鸡HSP70基因启动子区CpG岛的甲基化模式和水平不同,这可能导致两种鸡对应激反应产生不同的耐受程度。展开更多
[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with ...[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat.展开更多
[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of ...[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of （46.34±3.06） kg were randomly divided into three groups： con- trol group （protein level 15%, lysine level 0.83%）, experimental grnup 1 （protein level 13%, 0.83%）, and experimental group II （protein level 13%, lysine level 1.03）, each group had three replicates of four pigs. The trial lasted 35 d. [Result] The growth performance of Hainan black pig in grnup II had no significan! difference with that in contrail group （P〉0.05）. The apparent digestibility of chide protein and phosphorus of Hainan black pigs in Group II increased significantly （P〈0.05）, which were increased by 10.08% and 11.85% respectively compared to the control group; the apparent digestibility of calcium was increased, but the difference was not signifieant （P〉0.05）. [Conclusion] It will not reduce the growth performance of Hainan blaek pig when the protein level of diet is reduced by 2% and Ihe lysine level is increased to 1.03%. It is recommended that the diet can be used in the production of llainan black pig.展开更多
文摘为了获得鸡热休克蛋白70(heat shock protein 70,HSP70)基因启动子区CpG岛的甲基化状态,初步探索HSP70基因在肌肉组织生长发育中的作用,试验以文昌鸡和北京油鸡为试验材料,采用MassArray质谱法检测鸡胸肌中HSP70基因启动子区CpG岛的甲基化水平,比较分析文昌鸡和北京油鸡HSP70基因的甲基化差异。结果表明:在HSP70基因启动子中共检测到65个CpG位点的甲基化水平;文昌鸡胸肌生长发育过程中有8个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈下降趋势;北京油鸡胸肌生长发育过程中只有3个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈升高趋势,第120天时甲基化水平达到最高。说明文昌鸡与北京油鸡HSP70基因启动子区CpG岛的甲基化模式和水平不同,这可能导致两种鸡对应激反应产生不同的耐受程度。
基金Supported by Major Science and Technology Program of Hainan Province(ZDKJ2016017-01,ZDKJ2016017-03)
文摘[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat.
基金Supported by Technical Development Project of Provincial Scientific Research Institutes in Hainan(KYYS-2016-12)
文摘[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of （46.34±3.06） kg were randomly divided into three groups： con- trol group （protein level 15%, lysine level 0.83%）, experimental grnup 1 （protein level 13%, 0.83%）, and experimental group II （protein level 13%, lysine level 1.03）, each group had three replicates of four pigs. The trial lasted 35 d. [Result] The growth performance of Hainan black pig in grnup II had no significan! difference with that in contrail group （P〉0.05）. The apparent digestibility of chide protein and phosphorus of Hainan black pigs in Group II increased significantly （P〈0.05）, which were increased by 10.08% and 11.85% respectively compared to the control group; the apparent digestibility of calcium was increased, but the difference was not signifieant （P〉0.05）. [Conclusion] It will not reduce the growth performance of Hainan blaek pig when the protein level of diet is reduced by 2% and Ihe lysine level is increased to 1.03%. It is recommended that the diet can be used in the production of llainan black pig.
