Mutations in mitochondrial DNA(mtDNA)are maternally inherited and have the potential to cause severe disorders.Mitochondrial replacement therapies,including spindle,polar body,and pronuclear transfers,are promising st...Mutations in mitochondrial DNA(mtDNA)are maternally inherited and have the potential to cause severe disorders.Mitochondrial replacement therapies,including spindle,polar body,and pronuclear transfers,are promising strategies for preventing the hereditary transmission of mtDNA diseases.While pronuclear transfer has been used to generate mitochondrial replacement mouse models and human embryos,its application in non-human primates has not been previously reported.In this study,we successfully generated four healthy cynomolgus monkeys(Macaca fascicularis)via female pronuclear transfer.These individuals all survived for more than two years and exhibited minimal mtDNA carryover(3.8%–6.7%),as well as relatively stable mtDNA heteroplasmy dynamics during development.The successful establishment of this nonhuman primate model highlights the considerable potential of pronuclear transfer in reducing the risk of inherited mtDNA diseases and provides a valuable preclinical research model for advancing mitochondrial replacement therapies in humans.展开更多
In pressurized water reactor,fretting corrosion has become the main reason for the failure of 690TT heat exchanger tubes.The effect of temperature on the fretting corrosion behavior between 690TT tube and 405 stainles...In pressurized water reactor,fretting corrosion has become the main reason for the failure of 690TT heat exchanger tubes.The effect of temperature on the fretting corrosion behavior between 690TT tube and 405 stainless steel(SS)bar has been studied during 106 fretting cycles.The overall average coefficient of friction(COF)values descends with an increase in test temperature,while the width of worn scar becomes wider.The severest fretting corrosion happens when the test temperature is at 100℃.The wear mechanism differs at different test temperatures,from adhesive wear at room temperature to abrasive wear and delamination at 100℃,to abrasive wear at 200℃.Deformation slips,high residual strain concentration,and micro-cracks are found which are disadvantageous for the further service performance of the tubes.展开更多
基金supported by the National Natural Science Foundation of China (82021001,31825018)National Key Research and Development Program of China (2022YFF0710901)+3 种基金Shanghai Municipal Science and Technology Major Project (2018SHZDZX05)Strategic Priority Research Program of the Chinese Academy of Sciences (XDB32060100)Biological Resources Program of Chinese Academy of Sciences (KFJ-BRP-005)National Science and Technology Innovation 2030 Major Program 2021ZD0200900。
文摘Mutations in mitochondrial DNA(mtDNA)are maternally inherited and have the potential to cause severe disorders.Mitochondrial replacement therapies,including spindle,polar body,and pronuclear transfers,are promising strategies for preventing the hereditary transmission of mtDNA diseases.While pronuclear transfer has been used to generate mitochondrial replacement mouse models and human embryos,its application in non-human primates has not been previously reported.In this study,we successfully generated four healthy cynomolgus monkeys(Macaca fascicularis)via female pronuclear transfer.These individuals all survived for more than two years and exhibited minimal mtDNA carryover(3.8%–6.7%),as well as relatively stable mtDNA heteroplasmy dynamics during development.The successful establishment of this nonhuman primate model highlights the considerable potential of pronuclear transfer in reducing the risk of inherited mtDNA diseases and provides a valuable preclinical research model for advancing mitochondrial replacement therapies in humans.
基金supported by the National Natural Science Foundation of China (No.51771211)the Large-scale advanced PWR major projects (No.2017ZX06002006)+1 种基金the Liaoning Provincial Natural Science Foundation of China (No.20180540076)the open-ended fund of the CAS Key laboratory of Nuclear Materials and Safety Assessment (Institute of Metal Research,Chinese Academy of Sciences,China) (Nos.2019NMSAKF02 and 2017NMSAKF04)
文摘In pressurized water reactor,fretting corrosion has become the main reason for the failure of 690TT heat exchanger tubes.The effect of temperature on the fretting corrosion behavior between 690TT tube and 405 stainless steel(SS)bar has been studied during 106 fretting cycles.The overall average coefficient of friction(COF)values descends with an increase in test temperature,while the width of worn scar becomes wider.The severest fretting corrosion happens when the test temperature is at 100℃.The wear mechanism differs at different test temperatures,from adhesive wear at room temperature to abrasive wear and delamination at 100℃,to abrasive wear at 200℃.Deformation slips,high residual strain concentration,and micro-cracks are found which are disadvantageous for the further service performance of the tubes.
