Sex pheromones,which consist of multiple components in specific ratios pro-mote intraspecific sexual communications of insects.Plutella xylostella(L.)is a world-wide pest of cruciferous vegetables,the mating behavior ...Sex pheromones,which consist of multiple components in specific ratios pro-mote intraspecific sexual communications of insects.Plutella xylostella(L.)is a world-wide pest of cruciferous vegetables,the mating behavior of which is highly dependent on its olfactory system.Long trichoid sensilla on male antennae are the main olfactory sen-silla that can sense sex pheromones.However,the underlying mechanisms remain unclear.In this study,3 sex pheromone components from sex pheromone gland secretions of P xy-lostella female adults were identified as Z11-16:Ald,Z11-16:Ac,and Z11-16:0H in a ratio of 9.4:100:17 using gas chromatography-mass spectrometry and gas chromatography with electroantennographic detection.Electrophysiological responses of 581 and 385 long trichoid sensilla of male adults and female adults,respectively,to the 3 components were measured by single sensillum recording.Hierarchical clustering analysis showed that the long trichoid sensilla were of 6 different types.In the male antennae,52.32%,5.51%,and 1.89%of the sensilla responded to Z11-16:Ald,Z11-16:Ac,and Z11-16:0H,which are named as A type,B type,and C type sensilla,respectively;2.93%named as D type sen-silla responded to both Z11-16:Ald and Z11-16:Ac,and 0.34%named as E type sensilla were sensitive to both Z11-16:Ald and Z11-16:OH.In the female antennae,only 7.53%of long trichoid sensilla responded to the sex pheromone components,A type sensilla were 3.64%,B type and C type sensilla were both 0.52%,D type sensilla were 1.30%,and 1.56%of the sensilla responded to all 3 components,which were named as F type sen-silla.The responding long trichoid sensilla were located from the base to the terminal of the male antennae and from the base to the middle of the female antennae.The pheromone mixture(Z11-16:Ald:Z11-16:Ac:Z11-16:0H=9.4:100:17)had a weakly repellent effect on female adults of P xylostella.Our results lay the foundation for further studies on sex pheromone communications in P.xylostella.展开更多
基金This study was supported by the National Natural Science Foundation of China(Grant No.32130090,32300403)the Distinguished and ExcellentYoung Scholar Cultivation Project of Shanxi Agricultural University(2023YQPYGC05)+1 种基金the Leading Industrial and Engineering Research Project of Shanxi Agricultural University(Grant No.CYYL23-25)the Cultivation and Innovation Project of Plant Protection College of Shanxi Agricultural University(Grant No.ZBXY23B-13).
文摘Sex pheromones,which consist of multiple components in specific ratios pro-mote intraspecific sexual communications of insects.Plutella xylostella(L.)is a world-wide pest of cruciferous vegetables,the mating behavior of which is highly dependent on its olfactory system.Long trichoid sensilla on male antennae are the main olfactory sen-silla that can sense sex pheromones.However,the underlying mechanisms remain unclear.In this study,3 sex pheromone components from sex pheromone gland secretions of P xy-lostella female adults were identified as Z11-16:Ald,Z11-16:Ac,and Z11-16:0H in a ratio of 9.4:100:17 using gas chromatography-mass spectrometry and gas chromatography with electroantennographic detection.Electrophysiological responses of 581 and 385 long trichoid sensilla of male adults and female adults,respectively,to the 3 components were measured by single sensillum recording.Hierarchical clustering analysis showed that the long trichoid sensilla were of 6 different types.In the male antennae,52.32%,5.51%,and 1.89%of the sensilla responded to Z11-16:Ald,Z11-16:Ac,and Z11-16:0H,which are named as A type,B type,and C type sensilla,respectively;2.93%named as D type sen-silla responded to both Z11-16:Ald and Z11-16:Ac,and 0.34%named as E type sensilla were sensitive to both Z11-16:Ald and Z11-16:OH.In the female antennae,only 7.53%of long trichoid sensilla responded to the sex pheromone components,A type sensilla were 3.64%,B type and C type sensilla were both 0.52%,D type sensilla were 1.30%,and 1.56%of the sensilla responded to all 3 components,which were named as F type sen-silla.The responding long trichoid sensilla were located from the base to the terminal of the male antennae and from the base to the middle of the female antennae.The pheromone mixture(Z11-16:Ald:Z11-16:Ac:Z11-16:0H=9.4:100:17)had a weakly repellent effect on female adults of P xylostella.Our results lay the foundation for further studies on sex pheromone communications in P.xylostella.
