This paper summarizes and analyses the status quo of the sericultural standards system in China,and points out the characteristics and existed problems of sericultural standards in China. In addition,the future of the...This paper summarizes and analyses the status quo of the sericultural standards system in China,and points out the characteristics and existed problems of sericultural standards in China. In addition,the future of the sericultural standards system is discussed.展开更多
microRNAs(miRNAs)are 20–24 nucleotide(nt)RNAs that regulate eukaryotic gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs(mRNAs).To identify miRNA tar...microRNAs(miRNAs)are 20–24 nucleotide(nt)RNAs that regulate eukaryotic gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs(mRNAs).To identify miRNA target genes will help a lot by understanding their biological functions.Sophisticated computational approaches for miRNA target prediction,and effective biological techniques for validating these targets now play a central role in elucidating their functions.Owing to the imperfect complementarity of animal miRNAs with their targets,it is difficult to judge the accuracy of the prediction.Complexity of regulation by miRNA-mediated targets at protein and mRNAs levels has made it more challenging to identify the targets.To date,only a few miRNAs targets are confirmed.In this article,we review the methods of miRNA target prediction and the experimental validation for their corresponding mRNA targets in animals.展开更多
基金Supported by Modern Agricultural Industry Technology System(CARS-22)
文摘This paper summarizes and analyses the status quo of the sericultural standards system in China,and points out the characteristics and existed problems of sericultural standards in China. In addition,the future of the sericultural standards system is discussed.
基金supported by research grants from the National Basic Research Program of China(973 Program)(No.2005CB121004)the National Programs for High Technology Research and Development Program of China(863 Program)(No.2006AA10A119)Innovation Foundation for Graduate Students of Jiangsu Province and the National Natural Science Foundation of China(No.61001013).
文摘microRNAs(miRNAs)are 20–24 nucleotide(nt)RNAs that regulate eukaryotic gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs(mRNAs).To identify miRNA target genes will help a lot by understanding their biological functions.Sophisticated computational approaches for miRNA target prediction,and effective biological techniques for validating these targets now play a central role in elucidating their functions.Owing to the imperfect complementarity of animal miRNAs with their targets,it is difficult to judge the accuracy of the prediction.Complexity of regulation by miRNA-mediated targets at protein and mRNAs levels has made it more challenging to identify the targets.To date,only a few miRNAs targets are confirmed.In this article,we review the methods of miRNA target prediction and the experimental validation for their corresponding mRNA targets in animals.