Arthritis is a kind of chronic inflammatory autoimmune disease,which can destroy joint cartilage and bone,leading to joint pain,joint swelling,and limited mobility.Traditional therapies have many side effects or focus...Arthritis is a kind of chronic inflammatory autoimmune disease,which can destroy joint cartilage and bone,leading to joint pain,joint swelling,and limited mobility.Traditional therapies have many side effects or focus too much on anti-inflammation while neglecting joint repair.In this experiment,we combined Epigallocatechin gallate(EGCG)with extracellular vesicles derived from macrophages to treat rheumatoid arthritis.Sustained-release resulted in a significant decrease in chondrocyte expression of hypoxia-inducible factor 1-alpha,a decrease in apoptosis-related proteins Cytochrome C,Caspase-3,Caspase-9,and Bax.Molecular biological analysis showed that extracellular vesicles-encapsulated EGCG(EVs-EGCG)more significantly upregulated type II collagen expression by about 1.8-fold than EGCG alone,which was more beneficial for arthritis repair.Animal experiments revealed that these EGCG-coated extracellular vesicles significantly reduced swelling,decreased synovial hyperplasia,repaired cartilage,and attenuated arthritis-related pathology scores in arthritic rats.Measurement data showed that EVs-EGCG treatment reduced joint swelling by approximately 39.5%in rheumatoid rats.In vitro studies have shown that this EVs-EGCG can increase the expression of cartilage type II collagen and reduce apoptosis of chondrocytes.Moreover,it was demonstrated in vivo experiments to reduce cartilage destruction in rheumatoid arthritis rats,providing a solution for the treatment of rheumatoid arthritis.展开更多
基金supported via National Natural Science Foundation of China(No.82072076,81771987 and 82072165)Natural Science Foundation of Liaoning Province(No.201602309).
文摘Arthritis is a kind of chronic inflammatory autoimmune disease,which can destroy joint cartilage and bone,leading to joint pain,joint swelling,and limited mobility.Traditional therapies have many side effects or focus too much on anti-inflammation while neglecting joint repair.In this experiment,we combined Epigallocatechin gallate(EGCG)with extracellular vesicles derived from macrophages to treat rheumatoid arthritis.Sustained-release resulted in a significant decrease in chondrocyte expression of hypoxia-inducible factor 1-alpha,a decrease in apoptosis-related proteins Cytochrome C,Caspase-3,Caspase-9,and Bax.Molecular biological analysis showed that extracellular vesicles-encapsulated EGCG(EVs-EGCG)more significantly upregulated type II collagen expression by about 1.8-fold than EGCG alone,which was more beneficial for arthritis repair.Animal experiments revealed that these EGCG-coated extracellular vesicles significantly reduced swelling,decreased synovial hyperplasia,repaired cartilage,and attenuated arthritis-related pathology scores in arthritic rats.Measurement data showed that EVs-EGCG treatment reduced joint swelling by approximately 39.5%in rheumatoid rats.In vitro studies have shown that this EVs-EGCG can increase the expression of cartilage type II collagen and reduce apoptosis of chondrocytes.Moreover,it was demonstrated in vivo experiments to reduce cartilage destruction in rheumatoid arthritis rats,providing a solution for the treatment of rheumatoid arthritis.
