An Assessment Guide for English Language Teachers

LA Brief Introduction of the Workshop This workshop aims at improving language teachers＇ overall understanding of assessment since ＂assessment and teaching are partners in learning process＂ （Douglas, 2001： 421）. It is of crucial importance to raise the awareness of English Language Teachers of test ad assessment, the kinds of tests, validity and reliability of a test, traditional and alternative assessments, the cautions in constructing assessments and overall, the important role of tests in teaching and learning.

Effects of microbe-derived antioxidants on growth,digestive and aminotransferase activities,and antioxidant capacities in the hepatopancreas of Eriocheir sinensis under ammonia nitrogen stress

This study evaluated the effects of feeding a microbial-derived antioxidant diet(MA)for 56 days on the growth performance of Eriocheir sinensis,as well as on digestive and metabolic enzyme activity and antioxidant capacity under ammonia stress(400 mg/L ammonia nitrogen for 48 h).The weight gain at 28d and the specific growth rate at 56ds for crabs in the MA group were significantly higher than those in the control diet(C)group(p<0.05).Compared with the C group,the color of the hepatopancreas in the MA group was significantly improved(p<0.05).A significant decrease in the level of Hemolymph ammonia was found in the microbial antioxidant ammonia-N(MA_A)group(p<0.05),which may be related to the decrease of transaminase(AST,ALT,GDH)activities caused by MA diet(p<0.05).There is a significant increase in the digestive enzyme activities(Trypsin and Lipase),which reduced the accumulation of triglycerides in the hepatopancreas in the(MA_A)group(p<0.05).In addition,the antioxidant damage of crabs in hepatopancreas caused by ammonia nitrogen toxicity could also significantly be improved by MA fed in the(MA_A)group(p<0.05).microbe-derived antioxidants(supplemented with 0.2% in feeds)can protect the health of hepatopancreas,promote growth,and improve the ability to resist ammonia nitrogen stress for E.sinensis.

GPX4 mRNA levels in the polymorphonuclear neutrophils are negatively correlated with autoantibody production,disease activity,and lupus alopecia in systemic lupus erythematosus

Background::Ferroptosis is a type of regulated necrosis and glutathione peroxidase 4(GPX4)has been recognized as a key enzyme that protects against ferroptosis.However,the significance of GPX4 in polymorphonuclear neutrophils(PMNs)of systemic lupus erythematosus(SLE)has not been explored.So we examined GPX4 mRNA in PMNs and analyzed its association with serological and clinical features.Methods::A single center research from the Department of Rheumatology and Immunology of the Third Affiliated Hospital,Southern Medical University was conducted between December 2020 and September 2022.Real-time transcription-polymerase chain reaction analysis was used to determine the expression of GPX4 mRNA in PMNs from patients suffering from different rheumatoid immune diseases and healthy controls.The associations of GPX4 RNA levels in SLE patients with serological and clinical indicators were assessed by Spearman's correlation analysis.Receiver operating characteristic curve analysis was performed to evaluate the diagnostic value of GXP4 mRNA for SLE.Results::GPX4 mRNA levels were lower in SLE patients than those in healthy individuals,patients with rheumatoid arthritis and axial spondyloarthritis.GPX4 mRNA levels were negatively correlated with the number of positive autoantibodies(r=-0.3072,p=0.0300),anti-dsDNA antibody(r=-0.3654,p=0.0336),antinucleosome antibody(r=-0.4052,p=0.0263),erythrocyte sedimentation rate(r=-0.3773,p=0.0069),C-reactive protein(r=-0.4037,p=0.0036),and SLE disease activity index(r=-0.3072,p=0.0300).GPX4 mRNA levels were downregulated in patients with alopecia compared with patients without alopecia(p<0.05).The diagnostic capacity of GPX4 mRNA achieved high diagnostic accuracy(area under the curve=0.848)with sensitivity(78.00%)and specificity(80.95%).Conclusion::Downregulated GPX4 mRNA in PMNs of SLE patients is negatively associated with the production of antinuclear antibodies,disease activity,and lupus alopecia,suggesting an important role of ferroptosis in SLE,high diagnostic value of GPX4 mRNA in PMNs,and potential therapies targeting GPX4 for SLE patients,especially lupus alopecia.